고추역병균(Phytophthora capsici)은 고추 생육 전반에 걸쳐 병을 발생시켜 농가 소득에 큰 손실을 일으키고 있다. 고추 역병균 저항성은 양적 형질 유전자좌(Quantitative Trait Loci, QTL)에 의해 조절되며 주동 유전자는 고추의 5번 염색체에 존재한다고 보고 되었지만, 후보 유전자의 선발 및 저항성 유전자 규명 연구는 아직 초기 단계이다. 특히, 고추는 형질전환이 어려운 작물로써 병원균과의 상호작용 연구를 통한 저항성 유전자 동정에 제한이 많다. 반면 고추와 같은 가지과 작물인 담배(Nicotiana benthamiana)는 병원균 상호작용 모델로 알려져 형질전환을 통해 저항성 유전자 규명에 활용된다. 본 연구에서는 고추 역병 저항성 기작 규명을 위한 기초 연구로써, 식물 저항성 유사 유전자(Resistance Gene Analog, RGA)를 선발하고, 이들 유전자들에 대한 담배 형질전환 기법 최적화 연구를 수행하였다. 고추 5번 염색체에 존재하는 고추 역병 저항성 분자표지들을 분석하여 RGA 후보 유전자인 CaNBARC105, CaNBARC112 유전자를 동정하였다. 이들 유전자들에 대해 Agrobacterium tumefaciens를 매개체로 하여 고추 RGA가 삽입된 담배 형질전환체를 개발하였다. 형질전환 여부는 유전자 특이적인 서열을 이용한 genomic PCR과 RT-PCR 검증을 통해 이들 형질전환 된 담배들의 생육 및 발달에 영향이 없다는 것을 확인하였다. 본 연구는 향후 고추 병 저항성 후보 유전자들이 삽입된 담배 형질전환체는 고추 역병 저항성 유전자 규명 및 기작 연구에 기반이 될 것이다.

Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar (IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

전산유체역학을 사용하는 일반적인 선박의 저항성능 평가는 많은 시간과 비용이 필요하며, 이를 줄이기 위한 다양한 방법이 연구되고 있다. 선박의 주요 치수나 단면을 이용하는 기존의 방법들은 선형에 크게 좌우되는 저항성능을 추정하는데 한계가 있다. 본 논 문에서는 선형 격자의 기하학적 정보를 입력으로 선체 표면의 저항성능을 빠르게 추정할 수 있는 심층신경망 모델을 제안한다. Perceiver IO 기반의 제안하는 심층신경망 모델은 시간 단계별로 계산이 필요한 전산유체역학 기법과 달리 바로 저항성능 추정이 가능하며, 저속비 대선의 일종인 50K 탱커 선박을 대상으로 한 데이터집합에서 평균 1% 미만의 오차로 저항성능을 추정하는 결과를 보인다.

본 연구의 목적은 12주간 수중걷기운동이 비만 노인 여성의 신체조성, hs-CRP, HOMAIR 및 혈압에 미치는 영향을 알아보기 위하여 실시하였다. 체지방률 30% 이상인 비만 노인 여성 중 운 동군(10명), 대조군(10명)으로 총 20명으로 실시하였으며, 모든 검사항목은 동일한 방법과 조건으로 신 체조성, hs-CRP, HOMA-IR 및 혈압을 사전, 사후 총 2회 측정하였다. 수중걷기운동은 12주간, 주 3 회, 1회 50분, RPE 11-14 강도로 실시하였다. 측정변인에 대해 평균값과 표준편차(M±SD)를 산출한 후 사전 운동군과 대조군의 신체적 특성과 각 측정변인에 대한 동질성 검정을 위하여 독립 t-test를, 집 단 내의 사전·사후 평균치 변화에 대한 차이 검증은 종속 t-test를, 집단 간 차이에 대한 주효과 검정 및 집단 간 시기 간 상호작용 효과는 반복측정 분산분석을 이용하였다. 본 연구결과 운동군에서 신체조성, 체지방률(p<.05), 인슐린(p<.01), HOMA-IR(p<.01) 및 수축기 혈압(p<.05)이 유의하게 감소하였다. 따 라서 수중걷기운동이 비만 노인 여성의 비만 및 심혈관질환의 개선에 도움을 줄 수 있을 것으로 사료 된다.

The thermal shock resistance of cement composites with hollow glass microspheres (HGM) is investigated. Cement composites containing various concentrations of HGM are prepared and their properties studied. The density, thermal conductivity, and coefficient of thermal expansion of the composites decrease with increasing HGM concentration. A thermal shock test is performed by cycling between -60 and 50oC. After the thermal shock test, the compressive strength of the cement composite without HGM decreases by 28.4%, whereas the compressive strength of the cement composite with 30 wt% HGM decreases by 5.7%. This confirms that the thermal shock resistance of cement is improved by the incorporation of HGM. This effect is attributed to the reduction of the thermal conductivity and coefficient of thermal expansion of the cement composite because of the incorporation of HGM, thereby reducing the occurrence of defects due to external temperature changes.

PURPOSES : The aim of this study is to evaluate the stripping resistance of a bead coating via the Hamburg wheel tracking test and image analysis. METHODS : First, the stripping resistance of the bead coating was evaluated via the Hamburg wheel tracking test. A pneumatic wheel with a load of 175±2 N was used to simulate repeated skid cycles. Several bead coating mixtures with different numbers of coating layers, i.e., zero, one, two, three, and four layers, i.e., zero, one, two, three, and four layers,were conducted. Finally, an image analysis program was developed to analyze surface images captured from the Hamburg wheel tracking test. RESULTS : The results show that the samples with more coating layers exhibit higher stripping resistance. After 500 stripping cycles, the percentage of bead loss is 4% to 28%. At 80% bead loss, the mixture with one coating layer presents more skid cycles than the control sample without a coating layer. CONCLUSIONS : Incorporating a coating layer can improve the stripping resistance of glass beads under repeated skid cycles. Additionally, an image analysis program is established in this study to determine the percentage of bead loss caused by the stripping test.

Salinity is a major abiotic stress for crop plants that cause reduced food production. The application of plant growth-promoting microorganisms can improve the adaptability of plants to biotic and abiotic stresses. Therefore, in the current study, we aimed to enhance the mitigation effects of artificially applied rhizobacterium Klebsiella variicola AY13 on salinity stress in Trifolium repens L. The results suggested that AY13 innately produced indole-3-acetic acid and accelerated the morphological adaptation of T. repens to salt stress conditions. Furthermore, AY13 promotes a Trp-dependent pathway for indole-3-acetic acid (IAA) production, as adding L-tryptophan (L-Trp) increased IAA production in pure culture. AY13 improved plant growth by maintaining relative water content and minimizing water loss in excised leaves. Moreover, AY13 inoculation significantly reduced the endogenous levels of abscisic acid and antioxidants such as glutathione and catalase. These results suggest that AY13 plays a crucial role in reprogramming stress-related metabolism and enhancing plant stress tolerance.

최근 국제해사기구의 해양환경오염규제가 강화되어 오고 있다. 선박의 에너지 효율지수는 선박의 설계관점에서 매우 중요 한 지표이다. 더욱이 새롭게 건조되는 선박은 물론 기존 운항 선박에도 에너지 효율지수를 만족하도록 강화하고 있다. 이에 따라 운항 되고 있는 기존선박의 에너지 효율지수를 높이기 위해 선수 벌브개조, 운항 중 트림 최적화, 에너지 절감장치등 다양한 방법이 적용되 고 있다. 본 연구에서는 전산 유체역학을 이용하여 다양한 선수/선미 트림조건에서 선박의 저항성능을 계산하고 분석하였다. 이를 바 탕으로 최적화 된 트림조건에서 선박의 저항성능을 더욱 개선하기 위해 선수 벌브의 형상을 재설계하였다. 그 결과 정수 중에서 개선 된 벌브 형상을 적용한 경우, 유효마력이 약 5% 향상되는 것을 확인하였으며, 향후 파도 중에서 재설계된 벌브형상이 저항성능에 미치 는 영향을 조사할 예정이다.

Sealing treatment is a post-surface treatment of the plasma spray coating process to improve the corrosion resistance of the coating material. In this study, the effect of the sealing on the corrosion resistance and adhesive strength of the plasma spray-coated alumina coatings was analyzed. For sealing, an epoxy resin was applied to the surface of the coated specimen using a brush. The coated specimen was subjected to a salt spray test for up to 48 hours and microstructural analysis revealed that corrosion in the coating layer/base material interface was suppressed due to the resin sealing. Measurement of the adhesive strength of the specimens subjected to the salt spray test indicated that the adhesive strength of the sealed specimens remained higher than that of the unsealed specimens. In conclusion, the resin sealing treatment for the plasma spray-coated alumina coatings is an effective method for suppressing corrosion in the coating layer/base material interface and maintaining high adhesive strength.

콘크리트 충전강관은 국부좌굴을 방지하고 내화성이 향상되기 때문에 건설현장에서 많이 적용되며 휨성능을 향상시 키기 위해 강관 내부에 철근을 보강하여 사용한다. 그러나 철근은 부식되며 내구성이 저하되기 때문에 이를 대신할 소재에 대 한 연구가 진행되고 있다. 탄소섬유보강근은 철근에 비해 경량이며 고강도와 내부식성이 우수하다는 이점이 있다. 그러나 임계 온도가 250℃로 철근의 임계온도인 538℃에 비해 현저히 낮기 때문에 내화피복이 필요하다. 따라서 열전달해석을 통해 탄소섬 유보강근을 사용하였을 때 온도분포를 확인하고 P-M상관도를 도출하여 적용 가능여부를 확인하고자 한다. 해석결과 내화성능을 확보하기 위해 콘크리트 피복두께 40mm, 뿜칠내화피복재 30mm를 적용하거나 콘크리트 피복두께 60mm, 뿜칠내화피복재 20mm 를 적용하면 3시간 내화성능을 만족하는 것으로 평가되었다.

In case a spent nuclear fuel transport cask is lost in the sea due to an accident during maritime transport, it is necessary to evaluate the critical depth by which the pressure resistance of the cask is maintained. A licensed type B package should maintain the integrity of containment boundary under water up to 200 m of depth. However, if the cask is damaged during accidents of severity excessing those of design basis accidents, or it is submerged in a sea deeper than 200 m, detailed analyses should be performed to evaluated the condition of the cask and possible scenarios for the release of radioactive contents contained in the cask. In this work, models to evaluate pressure resistance of an undamaged cask in the deep sea are developed and coded into a computer module. To ensure the reliability of the models and to maintain enough flexibility to account for a variety of input conditions, models in three different fidelities are utilized. A very sophisticated finite element analysis model is constructed to provide accurate response of containment boundary against external pressure. A simplified finite element model which can be easily generated with parameters derived from the dimensions and material properties of the cask. Lastly, mathematical formulas based on the shell theory are utilized to evaluate the stress and strain of cask body, lid and the bolts. The models in mathematical formula will be coded into computer model once they show good agreement with the other two model with much higher fidelity. The evaluation of the cask was largely divided into the lid, body, and bottom, bolts of the cask. It was confirmed that the internal stress of the cask was increased in accordance with the hydrostatic pressure. In particular, the lid and bottom have a circular plate shape and showed a similar deformation pattern with deflection at the center. The maximum stress occurred where the lid was in the center and the bottom was in contact with the body. Because the body was simplified and evaluated as a cylinder, only simple compression without torsion and bending was observed. The maximum stress occurred in the tangential direction from the inner side of the cylinder. The bolt connecting the lid and the body was subjected to both bending and tension at the same time, and the maximum stress was evaluated considering both tension and bending loads. In general, the results calculated by the formulas were evaluated to have higher maximum stresses than the analysis results of the simplified model. The results of the maximum stress evaluation in this study confirms that the mathematical models provide conservative results than the finite element models and can be used in the computer module.