본 논문은 히스토그램과 그레이레벨 프로파일의 VS(valley size)를 이용한 문턱치 기반의 대두 선별알고리즘 을 제안한다. 대두의 변형은 크게 표면 변색 및 외형 변형으로 나눌 수 있다. 본 연구에서는 대두 개체를 건전립, 부 분변색립, 전면변색립, 표면변형립으로 구분하고 히스토그램 기반 문턱치와 VS기반 선별알고리즘을 이용하여 표면의 변색 및 변형에 따른 불량 대두와 건전립을 선별하였다. 제안한 방법은 대두와 같이 개체수가 많은 농작물의 실시간 선별을 목적으로 하여, 정수덧셈 연산기반의 선별 알고리즘으로 구현 되었다. 제안한 방법을 실제 선별에 적용한 결 과 선별의 정확도는 94.5%이며 기존의 문턱치 기반 대두 선별 알고리즘에 비교할 때 상대적으로 적은 연산량에서 동 등한 수준의 정확도를 나타내는 것을 확인하였다.

As the development environment is changing with the development of information communication technology, the systems that were used by each service became used with integration. In the process of integrating from existing legacy systems to new system, it should be smoothly integrated or shared, however, it cannot help holding existing technology or component due to significant cost burden for conversion. In this paper, it was not only classified by types with analyzing the various elements that make up legacy system but an approach and monitoring system were developed to each type. After System application results, data's information generated in each process is provided to other system in real time, so that it has not only secured the work efficiency and reliability but also it is made possible by integrating data in various formats for efficient data management, rapid search and tracking to history. With real-time monitoring system developed in this study, It can be very useful in a variety of industries which require real-time monitoring of distributed legacy system data.

This paper is concerned with an experimental research to control of random vibration caused by external loads specially in cable-stayed bridges which tend to be structurally flexible. For the vibration control, we produced a model structure modelled on Seohae Grand Bridge, and we designed a shear type MR damper. On the center of its middle span, we placed a shear type MR damper which was to control its vibration and also acquire its structural responses such as displacement and acceleration at the same site. The experiments concerning controlling vibration were performed according to a variety of theories including un-control, passive on/off control, and clipped-optimal control. Its control performance was evaluated in terms of the absolute maximum displacements, RMS displacements, the absolute maximum accelerations, RMS accelerations, and the total power required to control the bridge which differ from each different experiment method. Among all the methods applied in this paper, clipped-optimal control method turned out to be the most effective to reduces of displacements, accelerations, and external power. Finally, It is proven that the clipped-optimal control method was effective and useful in the vibration control employing a semi-active devices such MR damper.

A number of plating companies have been exposed to the risk of fire due to unexpected temperature increasing of water in a plating bath. Since the companies are not able to forecast the unexpected temperature increasing of water and most of raw materials in the plating process have low ignition temperature, it is easy to be exposed to the risk of fire. Thus, the companies have to notice the changes immediately to prevent the risk of fire from plating process. Due to this reason, an agile and systematic temperature monitoring system is required for the plating companies. Unfortunately, in case of small size companies, it is hard to purchase a systematic solution and be offered consulting from one of the risk management consulting companies due to an expensive cost. In addition, most of the companies have insufficient research and development (R&D) experts to autonomously develop the risk management solution. In this article, we developed a real time remote temperature monitoring system which is easy to operate with a lower cost. The system is constructed by using Raspberry Pi single board computer and Android application to release an economic issue for the small sized plating manufacturing companies. The derived system is able to monitor the temperature continuously with tracking the temperature in the batch in a short time and transmit a push-alarm to a target-device located in a remoted area when the temperature exceeds a certain hazardous-temperature level. Therefore, the target small plating company achieves a risk management system with a small cost.

Prevotella intermedia-specific quantitative real-time PCR (qPCR) primers were previously designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). However, the several clinical strains isolated from Korean populations are not detectable by the qPCR primers. The purpose of this study was to develop new P. intermedia-specific qPCR primers based on the rpoB. The specificity of the primers was determined by conventional PCR with 12 strains of P. intermedia and 52 strains (52 species) of non-P. intermedia bacteria. The sensitivity of primers was determined by qPCR with serial dilutions of the purified genomic DNAs (40 ng to 4 fg) of P. intermedia ATCC 25611T. The data indicated that only P. intermedia strains were detected by the P intermedia-specific qPCR primers (RTPiF2/RTPiR2); in addition, as little as 40 fg of P. intermedia genomic DNA could be detected. These results suggest that these qPCR primers are useful in detecting P. intermedia from the bacterial infectious lesions including dental plaque and oral tissue lesions.

The incidents related to transporting hazardous materials may cause serious impacts on neighborhood and surrounding areas. It is essential to have a real-time safe management system for incidents prevention of transporting hazardous materials. Currently, the system is not integrated into one channel, which makes it difficult to control an incidents response. Another problem is that event status is not appropriately shared among authorities having responsibilities taking down the incidents. This paper investigates previous studies covering the real-time safety management system for hazard material transports and suggests an integrated management system that helps communicate effectively and promptly.

본 논문에서는 대두(soybean)의 외관 검사를 위해 명암도, 지역분산 및 지역 명암도 차이에 기초한 대두 선 별 알고리즘과 이를 적용한 대두 선별 장치의 구현 방법을 제안한다. 제안한 선별 장치는 4개의 채널로 구분된 벨트 컨베이어를 이용하여 대두 시료를 정렬된 형태로 이송하고, 종단에서 낙하하는 대두들에 대해 배후조명(back-light)과 흑백의 라인스캔(line-scan) 카메라를 이용하여 영상을 획득한 후 실시간으로 대두의 외관을 양품과 불량으로 선별하 였다. 실시간 선별을 위해서 각 채널별로 전용의 DSP를 사용하였으며, 각각의 DSP에서는 채널별로 얻은 대두 영상 으로부터 명암도, 명암도의 지역분산, 지역명암도 차이에 기초하여 양품으로부터 얻은 10개의 판별 문턱치와 대두 클 래스별 3개의 특징벡터를 찾아 4개의 클래스로 구분된 대두 영상에 대한 양품과 불량 선별을 실시하였다. 선별 결과 처리량은 약 520kg/hour였고, 양품 선별 정확도 즉, 양품에 대한 양품 인식률은 97%이며 불량 선별 오류, 즉 불량을 양품으로 인식하는 비율은 4.3%를 나타냈다.

This study explains an agricultural mobile commerce case using Oauth Based Real-Time Information Sharing Technique according to the growth of the agricultural e-commerce market. Considering the characteristics of the agricultural market, it can confirm differential values with the existing agricultural mobile commerce market according to the functions and characteristics of its technique. We compare variables for introducing agricultural mobile commerce with the existing mobile commerce. Trust through information sharing with real-timefarming diaries, suitability and perceived ease of use variables are positively influenced, but influence on completeness and authority of information variables are relatively insufficient. Through this, we explained differential values of agricultural mobile commerce and suggested an applicable business model.

본 연구에서는 김치로부터 W. cibaria 0D17와 W. confuse 0D23를 생화학적 특성 분석과 16s rRNA 염기서열 분석을 통해 분리, 동정하였으며, W. cibaria 0D17와 W. confuse 0D23 배양 상등액이 L. monocytogenes에 대한 항균 효과가 있는 것으로 나타났다. 또한, 실시간 정량 PCR을 통해 W. cibaria 0D17 및 W. confusa 0D23가 L. monocytogenes 를 공동 배양했을 때의 생육억제 효과를 분석한 결과, 37℃에서는 생육 억제 효과가 있는 것으로 확인되었다. 따라서, 김치 유래 Weissella spp. 가 갖는 L. monocytogenes에 대한 항균 활성에 대한 기초 자료로 활용가능 할 것으로 판단되며, W. cibaria 0D17 및 W. confusa 0D23가 생산하는 bacteriocin 등의 항균 물질 특성에 대한 연구가 추가 적으로 진행될 필요가 있을 것이다.

본 연구에서는 식육원료 4종(소, 돼지, 말 및 닭)을 각 각 판별하기 위하여 real-time PCR을 이용한 판별법을 개발하였다. 종판별을 위한 유전자로는 미토콘드리아 유전자 중 12S rRNA와 16S rRNA 부분을 대상으로 하였으며, 특이 프로브의 Reporter dye는 FAM, Quencher dye는 TAMRA로 설계하였다. 개발된 프라이머 및 프로브 세트 로 유사종에 대한 비 특이적 signal의 생성 유무를 관찰하기 위하여 총 10종을 대상으로 특이성을 확인한 결과, 비 특이적 signal은 확인되지 않았다. 식육원료에 대하여 realtime PCR을 통한 식육 판별 시 식육 혼합 방법에 따른 CT값의 유의적인 차이는 없었다. 의도적 혼합 및 비의도적 혼입을 판별하기 위한 정량법 개발에서는 의도적 혼합의 경우 100% 식육과의 CT 값 차이가 4 cycle 이내이고, 비의도적 혼입일 경우 100% 식육과의 CT 값 차이가 6 cycle 이상이었다. 따라서 본 연구를 통하여 개발한 식육 원료의 의도적, 비의도적 혼입 판별법은 불량식품 유통 근절 및 소비자 인권보호에 크게 기여할 것으로 기대된다.

해상에서 발생하는 위기의 확률적인 평가결과는 확률(P=0.0~1.0)로 나타난다. 이러한 확률적인 위기평가결과는 위험한 정도를 쉽게 알기 위하여 일반적으로 평가의 기준이 되는 위기허용수준(Risk Acceptance Criteria, RAC)을 이용하여 지수(index)로 나타낸다. 현재 RAC은 3단계, 5단계, 7단계 등으로 구분하는데, 구분한 단계 수가 위기평가에 적합한지를 평가할 필요가 있음에도 불구하고 관련 연구가 전무한 실정이다. 본 연구에서는 RAC의 단계 구분 수에 따른 민감도(Sensitivity)와 민감도의 분포특성을 이용하여 최적의 RAC 구분 단계 수를 정할 수 있는 평가기법을 제안하였다. 사전 연구결과로부터 획득한 확률적인 위기평가 데이터에 대해서 제안한 기법을 적용한 결과, 민감도가 10배 이내로 형성되는 최적의 RAC 구분 단계 수를 결정할 수 있었고, 이를 통해서 제안한 방법의 유효성을 확인하였다.

The goal of this research was to develop a portable system that could be used to evaluate the quality of milk in real time at a raw milk production site. A real-time portable quality evaluation system for raw milk was developed to enable non-destructive quality evaluation of somatic cell count (SCC), fat, protein, lactose, and total solid (TS) in milk samples. A prediction model of SCC, fat, protein, lactose, and TS was constructed using partial least squares (PLS) and 200 milk samples were used to evaluate the prediction performance of the portable quality evaluation system and high performance spectroscopy. Through prediction model development and verification, it was found that the accuracy of high performance spectroscopy was 90% for SSC, 96% for fat, 96% for protein, 91% for lactose, and 97% for TS. In comparison, the accuracy of the portable quality evaluation system was relatively low, at 90% for SSC, 95% for fat, 92% for protein, 89% for lactose, 92% for TS. However, the measurement time for high performance spectroscopy was 10 minutes for 1 sample, while for the portable quality evaluation system it was 6 minutes. This means that the high performance spectroscopy system can measure 48 samples per day (8 hours), while the portable quality evaluation system can measure 80 (8 hours). Therefore, it was found that the portable qual ity evaluation system enables quick on-site quality evaluation of milk samples.

The bumblebee, Bombus terrestris, plays an important role as one of alternative pollinators since the outbreak of honeybee colony collapse disorder. Recently, pathogens and parasites such as viruses, bacteria and mites affecting the life span and fecundity of their host have been discovered in B. terrestris. In this study, in order to detect viral infection in B. terrestris, we collected B. terrestris adults and isolated total RNA for diagnostic PCR. The PCR primers specific for pathogenic viruses were newly designed and applied to gene amplification for cloning and detection. Capsid protein gene of black queen cell virus (BQCV) among examined viral genes was only successfully amplified from collected bumble bee adults and sequenced. To optimize the detection of capsid protein gene of BQCV, 4 regions in the capsid protein gene were selected and further analyzed in quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis revealed that capsid protein gene was directly detected with not more than 200 ng total RNA. This result suggests that an optimized detection via qRT-PCR can be applied for the rapid and sensitive diagnosis of BQCV infection in the field population as well as risk assessment of B. terrestris.

본 연구에서는 FCV 현탁액에 물리, 화학적 위생처리 후 복합효소처리라는 전처리과정을 적용한 뒤 real-time RTPCR법을 이용하여 살균효능을 분석하였다. RT-PCR 이전에 37oC에서 30분 동안 PK와 RNase A를 처리함으로써 UV, 열, 염소, 에탄올, 과초산계열 제품에 의해 불활성화 된 바이러스들은 음성 결과를 나타내었고, real-time RTPCR법을 통해 살균 효능을 정량분석한 결과, 복합효소처리를 했을 경우 무처리구보다 더 높은 살균 효능을 보이는 것을 확인할 수 있었다. 이로써 Nuanualsuwan S. 등11,18,29)의 선행연구에서와 같이 PK와 RNase A로 전처리하는 단계를 통하여 물리, 화학적 위생처리에 의해 손상되지 않은 바이러스가 RT-PCR 법에 의해 증폭되는 것을 방지함으로써 Real-time PCR법 에 대한 검출 감도를 높일 수 있음을 확인하였다. 또한, FCV를 검출하기 위해 사용된 RT-PCR과 real-time RT-PCR 두 방법 중에서도 real-time RT-PCR법이 가장 신속하면서도 민감도 높은 결과로 도출되었다. 따라서, 유전자 분석 이전에 복합효소처리는 물리, 화학적 위생처리에 의해 불활성화 된 바이러스의 RNA가 transcription 또는 증폭되는 것을 방지하기 위한 수단으로 real-time RT-PCR법과 결합 됨으로써 노로바이러스를 비롯한 식중독 바이러스를 검출 하는데 효과적으로 적용될 것으로 판단된다. 또한 식품현 장에서 전기영동 과정없이 신속하게 살아있는 바이러스만을 수치적으로 정량화함으로써 식품안전에도 기여할 것으 로 사료된다.

This paper suggests a specific model that could efficiently improve the interaction and the interface between WMS(Warehouse Management System) terminal and PDA terminal through real time processing in manufacturing shop. The proposed model shows that the new method can more efficiently perform to reduce processing time for shipping and receiving, compared with the current approach. As a result of the certain test among the main server, WMS system, and PDA terminal, it is noted in case of the new proposed system that the effects of proposed model are as follows: (a) While the receiving lead time for carrying by the current method was 2 hours, the receiving lead time by the new method was 20 minutes. (b) While the shipping lead time for carrying by the current method was 1 hours, the shipping lead time by the new method was 15 minutes. (c) While the inventory rate of accuracy by the current method was 85%, the inventory rate of accuracy by the new method was 98%.

Norovirus causes acute gastroenteritis in all age groups and its food poisoning outbreaks are rapidly increasing in Korea. Reverse transcription-polymerase chain reaction (RT-PCR) is most widely used for the rapid detection of foodborne viruses due to high sensitivity. However, the false positive results of RT-PCR obtained against already inactivated viruses could be a serious drawbacks in food safety area. In this study, we investigated a method to yield true positive RT-PCR results only with alive viruses. To decompose the RNA genes from dead viruses, the enzymatic treatments composed of proteinse K and Ribonuclease A were applied to the sanitized and inactivated virus particles. Another aim of this study was to quantify the efficiencies of several major sanitizing treatments using realtime RT-PCR. Feline calicivirus (FCV) that belongs to the same Caliciviridae family with norovirus was used as a surrogate model for norovirus. The initial level of virus in control suspension was approximately 104 PFU/mL. Most of inactivated viruses treated with the enzymatic treatment for 30 min at 37oC were not detected in RT-PCR, Quantification results to verify the inactivation efficiencies of sanitizing treatments using real-time RT-PCR showed no false positive in most cases. We could successfully develope a numerical quantification process for the inactivated viruses after major sanitizing treatments using real-time RT-PCR. The results obtained in this study could provide a novel basis of rapid virus quantification in food safety area.