캐비어에 대한 수요가 증가하면서 캐비어 종류의 진위 여부를 확인 할 수 있는 분석법 마련이 필요해졌다. 본 연 구에서는 캐비어 종류을 나누는 철갑상어 종 특이 KASP 마커를 개발하였고 모니터링을 통해 국내 유통중인 불법 캐비어 제품을 확인하고자 하였다. 본 연구에서는 16S ribosomal RNA gene, cytochrome b gene, cytochrome c oxidase subunit I gene, cytochrome c oxidase subunit II gene, NADH dehydrogenase subunit 5 gene에서 철갑상어 종 특이적인 SNP를 선정하고 이를 표적하는 KASP 마커 11종을 개발하였다. 개발한 KASP 마커를 이용하여 한국 의 온라인 마켓에서 유통중인 캐비어 10종을 모니터링 분 석한 결과 2개의 제품에서 제품에 표기된 철갑상어 종과 다른 것으로 확인하였으며 두 제품 모두 실제보다 더 비 싼 캐비어로 둔갑하여 판매한 것으로 확인되었다. 본 연 구를 통해 제작한 KASP 분석방법으로 유통되는 캐비어 종류 분류가 가능하였고 모니터링을 통해 국내 유통되고 있는 불법 캐비어 제품도 확인하였다. 이에 따라 본 연구 에서 개발한 SNP기반 KASP 분석법으로 불법 캐비어 제 품 유통 근절에 기여 할 수 있을 것으로 기대한다.

The objective of this study was to investigate the suitable method for rapid establishment of grassland according to forage species, mixed pastures and installation of mesh at 35 degree angle of cutting area in the middle region of Korea. In agronomic characteristics after wintering, vegetation coverage of Tall fescue(TF) was 74% in monoculture, which was the highest among 3 forage crops. Meanwhile average vegetation coverages of monoculture and mixed pasture were 67 and 92% in treated of mesh, which were 4 and 18% higher than untreated of mesh, respectively. In botanical composition, TF of monoculture and Orchardgrass(OG) and TF oriented mixed were indicated over 94% forage coverage. Meanwhile forage coverages Kentucky bluegrass(KBG) and Red top(RT) of monoculture and Perennial ryegrass(PRG) oriented mixture were 89, 81 and 92% in treated of mesh, which were 7, 6 and 5% higher than untreated of mesh, respectively. In forage productivity, dry matter yield(DMY) of TF(12,537kg/ha) and KBG(11,897kg/ha) of monoculture were significant(p<0.05) higher than RT(9,604kg/ha). Meanwhile DMY of OG(12,227kg/ha), TF(12,823kg/ha) and PRG(11,871kg/ha) oriented mixed were not significant difference(p>0.05). In forage quality of monoculture, in the first year, crude protein of KBG was 13.6%, which was the highest among 3 forage species. Also neutral detergent fiber(NDF) of TF was 56.5%, which was the lowest among 3 forage species. In mixed pasture, in the second year, NDF and acid detergent fiber of PRG oriented mixed were 56.5 and 34.3%, respectively, which was the lowest among 3 mixed pasture. In conclusion, forage species TF was more suitable on initial rootage and continuous forage coverage, OG and TF oriented mixed were more suitable on continuous forage coverage. Also the installation of mesh showed positive effects on initial rootage and maintain forage ratio.