본 연구는 12주간 운동 강도 차이에 따른 복합운동 수행이 55-64세의 폐경 후 5년 이내 비만 중년 여성의 혈중지질, 알부민 및 FFA 수준에 미치는 영향을 분석하였다. 대상자는 중강도 운동군(MIG, n=10), 고강도 운동군(HIG, n=10)으로 구성하여 탄력밴드를 이용한 저항운동 및 트레드밀을 이용한 유산 소 걷기 운동을 주 3회, 1회 운동시간 60분간 실시하였다. 자료처리는 측정항목에 대한 평균값(M)과 표준 편차(SD)를 산출하였고, 그룹 및 시기 간 상호작용 효과 검증은 two-way repeated measures ANOVA를 실시하였다. 상호작용 효과를 포함하여 필요에 따라 그룹 내 시기 간 차이 검증은 paired t-test를 실시하였 고, 그룹 간 차이 검증은 independent t-test, 각 항목별 통계적 유의수준은 .05로 설정하였다. 그 결과, 혈 중지질 중 TG는 상호작용 효과(p<.05)가 나타났고, 알부민은 통계적으로 유의한 차이가 없었으며, FFA는 상호작용 효과(p<.05) 및 그룹 내 MIG, HIG 모두 감소하였지만, MIG에서 더 많은 감소가 나타났다. 이러 한 결과는 규칙적인 운동의 수행이 폐경 후 비만 중년 여성의 비만의 개선 및 예방에 효과적이라고 사료되 며, 특히 중강도 운동은 고강도 운동보다 TG 및 FFA에 더 많은 효과를 나타낸다는 것을 입증하였다. 따라서, 노년기로 넘어가기 전 꾸준한 운동 수행을 통해 비만을 개선하여 건강한 삶을 영위할 수 있도록 지속적 인 운동 수행을 권장한다.

소음 스트레스로 인한 뱀장어(Anguilla japonica)의 영향을 파악하기 위하여 스트레스 지표로 사용되는 코티졸, 포도당, 알부민 및 glucocorticoid receptor(GCR) 유전자의 발현 양을 측정, 분석하여 노출되지 않은 대조구와 비교하였다. 그 결과, 알부민은 노출 1시간 후에 낮은 값을 보인 반면 코티졸과 포도당은 대조구에 비해 매우 큰 차이를 보이며 높게 나타났다. GCR 유전자의 조직 발현 결과 간, 아가미, 근육 및 소장에서 많이 발현하였다. 소음 노출에 따른 시간의 변화에서 간과 아가미 근육과 소장에서 발현이 감소하는 양상을 나타내었다. 실험결과 뱀장어의 glucocorticoid receptor 유전자의 발현변화가 소음 스트레스로 인한 영향을 파악하는데 유용한 지표가 될 수 있음을 확인하였다.

This study was undertaken to find out the effect of cholesterol and serum albumin on sperm ability and lipid peroxidation levels period to the liquid storage of miniature pig sperm. Ejaculated semen from miniature pigs was collected by gloved-hand method into a pre-warmed () thermos bottle, and extended with Modena solution {with and without BSA, methyl-beta-cyclodextrin (-cholesterol) and cholesterol loaded cyclodextrin (+cholesterol)}. Each semen was assessed for viability (SYBR-14/PI staining) and acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining at 1, 3, 5, 7 and 10 days of storage. At for the effects of cholesterol and serum albumin on lipid peroxidation, semen were incubated with (), and lipid peroxidation level were measured by flow cytometry using the lipid peroxidation reporter probe . The result, lipid peroxidation level in sperm added with cholesterol were lower in compared to the added sperm with serum albumin. Also, added cholesterol to sperm had significant (p<0.05) higher viability when storage for 7 and 10 days and lower when 10 days of storage percentage of acrosome-reacted sperm (AR pattern) in acrosome state as say result compared to other treated groups. In conclusion, role of cholesterol during lipid storage in miniature pig spermatozoa was protected boar spermatozoa from lipid peroxidation prior to lipid storage. Addition serum albumin during lipid storage in sperm may be induce sperm membrane damage by lipid peroxidation. Therefore, addition of cholesterol to miniature pig sperm will be lead to extension of liquid storage periods.

The aim of the study were to investigate the influences of krill (Euphausia superba) meal on the body weight, lipid metabolism functional improvement, blood glucose level, protein component in the sera of rats which fed experimental diets for 5 weeks. Serum concentrations of total cholesterol, Low-Density Lipoprotein (LDL)-cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL) and blood glucose were higher in the control diet group (G1 group) than the control diet plus 10% krill meal group (G2 group), the control diet plus 20% krill meal group (G3 group), the control diet plus 30% krill meal group (G4 group), and a general dose and time independent one-way analysis of variance was performed to assess efficacy. Conversely depending on the content of krill meal for the High-Density Lipoprotein (HDL)-cholesterol level, it showed higher results. The concentrations of total protein, albumin and globulin in sera, there were not significant difference among the groups (p<0.05). The results indicate that a krill meal diet effectively inhibited increases in lipid elevation, blood glucose level in the sera of rats.

Background : Nowadays obesity has increased dramatically in developed countries which lead various metabolic disorders such as type 2 diabetes, hypertension, cancer, and the risk of stroke. Thus, looking forward a new chemical entity from natural sources which have a strong potential of anti-lipid regulation activity. Recently, protein based nanomedicine offers a new approach to treat a number of diseases including metabolic disorder such as obesity. In this study we evaluated the anti-lipid regulation effect of nano-carrier Bovine serum albumin and Mesoporous silica (MSNPs) conjugated ginsenoside F1 in 3T3-L1adipocytes and HepG2 hepatocytes. Methods and Results : BSA incorporated drugs has been shown to protect the drug degredation as well as improvement bioavalilability. To assess the anti- adipogenic effect of BSA-F1 and MSNPs-F1 cell viability was investigated in different time point of cell cycle growth and the intracellular lipid accumulation was evaluated in mature adipocytes and hepatocytes by Oil red staining assay. In addition, transcriptional gene regulation was quantified by the real time PCR for targeting adipogenic genes such as PPARγ, STAT3, CEBPα, ap2 and aP2 as well as hepatogenic genes such as ACC, AMPK, FAS and PPARα. Moreover, protein expression was evaluated by immunoblotting assay. Conclusion : Altogether, the above results were confirmed that BSA-F1 at dose 25 μM exhibited the anti-adipogenesis effect by downregulating the major transcriptional factors PPAR γ/STAT3 in signalling in 3T3-L1 mature adipocytes and upregulated the fatty acid oxidative AMPK signaling in fatty acid induced HepG2 hepatocytes.