This study investigated the substitution effect of phosphate and isolated soybean powder (ISP) by Allomyrina dichotoma larvae powder (AP) in emulsion sausage. The sausages were prepared for five treatments: 1) positive control (PC): sausages manufactured with sodium pyrophosphate (0.3%) and ISP (1%); 2) negative control (NC): sausages manufactured without sodium pyrophosphate, and ISP; 3) phosphate replacement (PR): sausages manufactured with ISP and AP (1%); 4) ISP replacement (IR): sausages manufactured with sodium pyrophosphate and AP; 5) phosphate and ISP replacement (PIR): sausages manufactured with AP. The 1% AP extract showed DPPH radical scavenging (45.65±2.468%) and metal chelating (22.46±3.559%) activity. The high pH value of AP (8.03) increased the pH of the meat batter of PR, IR, and PIR (p<0.05), but there was no inhibitory effect on cooking loss. Cooking loss of IR was not significantly different with PC due to phosphate. The addition of AP decreased the L* and a* values and increased the b* values, and decreased hardness, chewiness, and springiness of PR, IR, and PIR (p<0.05). AP did not inhibit lipid oxidation in sausages, in contrast to phosphate. In sensory evaluation, the addition of AP resulted in low scores for all sensory parameters of the sausages, although most panels had normal or positive awareness (p<0.05). Aroma was more influential on samples than color. Overall, AP did not replace phosphate and ISP in the sausages. Therefore, processes such as eliminating unique aroma and color and increasing solubility through particle size control are necessary to use AP as a protein source.
Imago"s flight activities of Protaetia brevitarsis were from mid June to early September, and those of Allomyrina dichotoma were from mid June to late August. These activities were mainly influenced by amount and duration of rain. They were more active when there was small amount and short period of rain in a year. Distribution depth of the 3<SUP>rd</SUP> larvae of P. brevitarsis were not affected to the temperature. But A. dichotoma were sensitively react to the temperature, almost larvae were near the surface when high temperature. The distribution of P. brevitarsis was not affected by humidity, but most of A. dichotoma were near the surface when high humidity. Larvae of P. brevitarsis usually ate their dead individuals. When we supplied as food the pork, chicken, fresh mackerel, bread, apple, and pear. They took all of these food but larvae of A. dichotoma took only bread. Two species were lived in similar environment but those feeding habit was very different.