This study was conducted to evaluate the effects of ImmunoSEB as an immune-booster additive on the performance of broiler chicks. A total of 1150 broiler chickens were randomly allotted to three treatments on the basis of body weight (10 pens per treatment with 40 broilers in each pen): the control (CON), CON + ImmunoSEB 0.025% in feed (SEB25), and CON + ImmunoSEB 0.050% in feed (SEB50). The experiment was conducted for d 42 in three phases (phase 1, d 0–14; phase 2, d 15–28; and phase 3, d 29–42). There were significant differences in the average daily gain (ADG) and feed intake. The ADG at d 14 in the SEB50 treatment was greater than that in the CON treatment. The overall ADG in the SEB50 treatment was greater than that in the CON treatment. During d 0–14, the feed intake of chickens in the SEB50 treatment increased compared to that in the CON treatment. The crude protein and lysine digestibility improved in the SEB25 and SEB50 treatments compared to those in the CON treatment at d 28. Superoxide dismutase concentration significantly increased in the SEB50 treatment compared to that in the CON treatment. The interleukin-1β and tumor necrosis factor-α concentrations were higher in the cecum of chickens in the CON treatment than in the SEB25 and SEB50 treatments. A lower population of E. coli was detected in the ileum and cecum of broilers fed the SEB50 diet compared to those of broilers fed the CON diet. The overall result showed the beneficial effects of using ImmunoSEB at a dose of 0.050% in broiler chickens.
본 연구는 폭염 스트레스(extreme heat stress, EHS, 33±2℃)에 노출된 브로일러에서 대두유, 당밀, 아미노산, 비타민 C를 적절하게 함유하는 폭염사료 (extreme heat stress diet, EHSD)급여가 짧은 사슬지방산 및 성장능력에 미치는 영향을 조사하였다. 부화 당일 Ross 308 병아리 500 마리를 5처리구로 완전임의배치 하였다. 각 처리구는 4반복을 가졌으며 각 반복은 25마리씩 배치하였다. 처리구는 역전점등과 함께 T1 (일반환경+기초사료 basal diet, BD), T2 (EHS+BD), T3 (EHS+BD 내 우지를 대두유로 대체 및 당밀 2%를 함유하는 EHSD), T4 (EHS+BD 내 우지를 대두유로 대체, 당밀 2%, 메치오닌과 라이신을 각각 일반사료의 1.5배씩 함유하는 EHSD), T5 (EHS+BD 내 우지를 대두유로 대체, 당밀 2%, 메치오닌과 라이신을 각각 일반사료의 1.5배씩 추가 및 비타민 C 200 ppm을 함유하는 EHSD)로 구분하였다. 브로일러의 증체량은 T1, T2와 비교할 때 T4, T5에서 유의하게 증가하였다. 그리고 면역기관, F낭, 흉선, 비장의 무게는 서로 비슷하였다. 또한 혈청 IgG, IgA, IgM 농도는 T4, T5가 T1, T2에 비교할 때 높았으나 코르티코스테론 농도는 유의하게 낮아졌다. 특히 맹장의 Lactobacillus는 T4, T5가 T1, T2에 비해서 증가하였으나 Escherichia, Coliform, Total aerobic bacteria는 오히려 유의하게 감소하였다. 초산, 프로피온산 및 총 짧은 사슬지방산 함량은 T4, T5가 T1, T2와 비교할 때 유의하게 높았다.
The objective of this study was to determine the feeding effect of a diet containing Cordyceps with fly pupa on the changes in blood lipid profile from broiler chickens, fatty acids and thiobarbituric acid reactive substances (TBARS) in chicken meat. A total of 360 chicks (Ross strain 308) were divided on hatch day into four treated groups with respect to a 35-day feeding regimen: T1 (control group), T2 (2.0% Cordyceps with fly pupa), T3 (3.5% Corceps with fly pupa) and T3 (5.0% Cordyceps with fly pupa). Blood triacylglyceride and total cholesterol level was significantly decreased by 5.32-10.63% and 9.23-12.62%, respectively, in groups T2, T3 and T4 when compared to T1 (p<0.05). Water holding capacity was significantly highest in T2 (p<0.05), while there were no significant differences among groups T2, T3 and T. In chicken meat, the ratio of saturated fatty acid to unsaturated fatty acid was high in the T3 and T4 groups, the ratio of n-6 to n-3 fatty acid was low in the T2, T3 and T4 groups and oleic acid (18:1n-9) was high in the T2, T3 and T4 groups, when compared to T1 (p<0.05). TBARS tended to increase according to the storage time (in days), and was significantly lower in the chicken thigh muscles with skin in groups T2, T3 and T4 as compared to T1 (p<0.05). These results suggest that a diet containing 2.0-3.5% of Cordyceps with fly pupa may decrease blood lipid and improve both the shelf-life and quality of broiler chicken meat.