반추 동물의 장내에서 발생하는 메탄은 주요 온실가스로, 배출을 완화하기 위한 다양한 연구가 활발히 진행되고 있다. 본 연구는 2,2’-Dithiodiethanesulfonic acid (DIA)가 in vitro 반추위 발효 특성과 가스 발생에 미치는 영향 평가를 통해 가공 방법 및 첨가 수준을 결정하고자 실시되었다. 실험 처리구는 대조구(무첨가), 양성 대조구(3-nitrooxypropanol, 120ppm), 그리고 원형-DIA와 가공형-DIA를 각각 60ppm, 120ppm, 240ppm 농도로 설정하여 진행하였다. 실험 결과, 원형-DIA의 첨가 수준이 증가함에 따라 총 가스와 메탄 발생량이 Linear하게 감소하였으며, 120ppm과 240ppm 농도에서 대조구 대비 유의적으로 감소하였다. 또한, 원형-DIA와 가공형-DIA의 첨가에 따라 총 휘발성 지방산 생성량은 대조구와 유의적인 차이가 없었다. 원형-DIA 240ppm 농도가 반추위 메탄 저감제로서 유망한 효과를 보였음을 확인할 수 있었다. 그러나, DIA의 메탄 저감을 규명하기 위한 미생물 분석 및 DIA의 급여 실험을 위한 효과적인 부형제 개발을 위한 연구가 필요하다.

The concentrations of odor and volatile organic compound (VOC)-inducing substances were measured using selected ion flow tube mass spectrometers (SIFT-MS). SIFT-MS can continuously measure the concentration of odor-causing substances and VOCs in real time without pre-treatment steps. Measurements were conducted during the day and at night at 10 spots in the chemical block of the Sihwa industrial complex. Similar measurement results were observed in the daytime and nighttime for materials except methyl ethyl ketone with high concentrations. A high concentration of hydrogen sulfide was also measured at night. It is expected that an amount of emissions of VOCs and odor-causing substances under the absence of inspection can be traced if measured at other industrial complexes in vulnerable times.

세포간층 lignin은 2차벽 lignin(end-wise-polymer)에 비해 축합도가 높으며 guaiacyl type의 phenol성 수산기등 말단기를 많이 갖는 2차벽 lignin보다 저분자량의 bulk polymer형 lignin이다.

Potentilla kleiniana is a perennial herb beloning to Rosaceae family. Herein we investigated the effect of light intensity, light quality and chemical elicitor on plant growth and the accumulation of bioactive compounds in P. kleiniana. After 60 days of cultivation under different shading level [0% (200 μmol·m -2 ·s -1 ), 35% (95 μmol·m -2 ·s -1 ), 55% (65 μmol·m -2 ·s -1 ), 75% (40 μmol·m -2 ·s -1 )] in the greenhouse, chlorophyll and carotenoid content were the highest under 35% treatment, however, plant height, leaf number and biomass were the highest under non-shading. As a result of cultivation among strong light condition as a control, florescence and three mixture light sources [red:white:blue (RWB) = 8:1:1, red:blue (RB) = 8:2, red:green:blue (RGB) = 8:1:1] as treatments in plant growth chamber (25 ± 2℃, 185 ± 3 μmol·m -2 ·s -1 ), growth, biomass, chlorophyll content low difference between total phenolic compouds and flavonoid content were higher under RWB treatment. DPPH radical elimination ability was the highest under all treatments especially florescence and RGB treatment except control. As a result of treating chemical elicitor [salicylic acid (SA), methyl jasmonate (MeJA)] concentration (0, 50, 100, 200 μM) respectively, plant height, petiole diameter and biomass were higher under non-treatment, MeJA 50 μM. It was investigated that fresh weight and dry weight under MeJA 50 μM treatment were especially a little high. Total phenolic compounds and flavonoid content of SA 50 μM treatment was the highest but DPPH radical elimination ability was significantly the highest under MeJA 200 μM (88.65%) and MeJA 50 μM (87.84%) treatment. Thus, this study suggested that we determined optimal shading and light quality in the greenhouse and plant growth chamber also confirmed bioactive compound content, antioxidant ratio increase according to different chemical elicitation concentration.

In this study, the chemical properties and phenolic compound of blueberry, bokbunja and mulberry and their pomace were determined to develop them as functional food materials. Water content of individual whole berry was ranged from 84.25-86.20%, and water content was significantly high in whole berries rather than their pomace (p<0.01). Additionally, each berry and its pomace’s pH was 3.32-5.18. Among them, whole mulberry showed the highest pH which is 5.18 (p<0.01). Total polyphenol and flavonoid contents were the greatest in blueberry pomace and they were 24.81 mg/g and 2.13 mg/g, respectively (p<0.01). However, mulberry pomace generated the greatest anthocyanin content compared to others (p <0.01). In phenolic compound profiles, cyanin chloride was detected in mulberry and bokbunja. Epigallocatechin, gallocatechin and isorhamnetin were found only in blueberry. Catechin (hydrate) and epicatechin were greater in pomaces than whole berries except blueberry (p<0.01), otherwise, significantly great rutin (trihydrate) and quercetin contents were found in whole berries as compared to their pomace except blueberry (p<0.01). Gallic acid was significantly greatest in mulberry (p< 0.01) and quercetin 3-D-galactoside was significantly greatest in blueberry (p< 0.01). Apigenin and luteolin were traced in mulberry, and mulberry pomace showed greater apigenin and luteolin contents than whole mulberry (p<0.01). Naringenin was greater in pomaces than whole berries (p <0.01). As a result, it was found that all berry extracts used in this study were able to be applied as