This study examined the growth performance and field evaluation of the dual herbicide-resistant transgenic creeping bentgrass plants. The effect of glyphosate treatment on the herbicide resistance of the transgenic creeping bentgrass plants was determined, and the non-transgenic control plant withered at the concentration 11 μg/mL or higher whereas the transgenic creeping bentgrass plants survived the treatment at the concentration of 3,000 μg/mL, and the increase of the plant length was repressed as the glyphosate treatment concentration was increased. At field evaluation, glufosinate-ammonium and glyphosate were simultaneously treated to investigate the weed control effect. The results showed that more than 90% of the weeds withered four week after herbicide treatment, while the transgenic creeping bentgrass plants continued to grow normally. Therefore, the dual herbicideresistant creeping bentgrass plants may be able to greatly contribute to the efficiency of weed control and to the economic feasibility of mowing in places such as golf courses.
월동기간 동안 zoysiauass [Zoysia matrella (L.) Merr]와 creeping bentgrass(Agrostis palustris Hunds)의 뿌리에서 저온 스트레스에 대한 생리학적 반응을 규명하기 위해 지질과산화 정도와 효소적 항산화 반응 및 탄수화물의 변화를 조사하였다. 1월 말 Creeping bentgrass의 뿌리 고사율은 zoysiagrass 보다 약 20% 현저히 높게 나타났다. Creeping bentgrass의
본 시험은 제주지역에서 지렁이분 시비량 차이(0, 150, 300, 450, 600kg/10a)에 따른 한지형 잔디(Creeping bentgrass)의 식생의 변화를 검토하고, 지렁이분 적정시비량을 구명하기 위하여 2004년 3월 21일부터 7월 10일까지 시험하였다. 초장은 무비구에서 지렁이분 600kg/10a까지 16.7cm에서 20.2cm로 커졌으나, 지렁이분 450kg과 600kg/10a 시비구에서는 유의한 차이가 없었다. 근장, 엽록소는 초장
본 시험은 모래입경의 차이(0.3-0.5, 0.5-0.8, 0.8-1.0, 1.0-1.5 및 1.5-2.0mm)에 따른 한지형 잔디의 식생의 변화를 구명하기 위하여 2004년 3월 21일부터 7월 9일까지 시험하였다. 초장은 모래입경 0.3-0.5mm에서 22.5cm로 커졌으나, 모래입경이 굵어짐에 따라 초장은 점차적으로 작아져서 모래입경 1.5-2.0mm 처리구에서 초장은 19.7cm였다. 근장 및 엽록소 함량은 초장 반응과 비슷한 경향이었다. 엽중과
본 시험은 제주지역에서 파종량 차이(4, 6, 8, 10 및 12kg/10a)에 따른 Creeping bentgrass의 식생변화를 구명하기 위하여 2004년 3월 21일 부터 7월 7일까지 수행한 결과를 요약하면 다음과 같다. 초장은 4kg/10a 파종에서 19.6cm로 파종량이 12kg/10a로 증가됨에 따라 30.4cm로 커졌으나, 10kg/10a과 12kg/10a 파종구간에는 차이가 없었다. 근장 및 엽록소 측정치는 파종량 차이에 따라 초장반응과
제주지역에서 파종기 이동(3월 16일26일, 4 월 5일15일25일)에 따른 Creeping bentgrass 의 식생변화를 구명하기 위하여 2004년 3월 16 일부터 7월 6일까지 시험하였다. 초장은 3월 16 일 파종에서 22.7cm 이였으나, 만파할수록 초장은 짧아져서 4월 25일 파종에서 초장은 16.6cm 였고, 근장 및 엽록소 측정치는 초장반응과 비슷한 경향이었다. 파종기가 3월 16일에서 4월 25일로 지연됨에 따라 엽중은 1,373kg에
본 시험은 제주지역에서 한지형 잔디의 적정 질소 분시 횟수를 구명하기 위하여 2004년 3월 26일부터 7월 8일까지 질소시비량을 20kg/10a로 고정하고, 15일 간격으로 1회에서 5회까지 분시하여 잔디의 식생변화를 조사하였다. 초장은 4~5회 분시에서 각각 21.8cm, 21.9cm로 가장 길었으나, 시비횟수가 적어짐에 따라 초장은 참아졌다. 근장과 엽록소는 초장반응과 비슷한 경향이었다. 질소 시비횟수가 1회에서 5회로 증가함에 따라 엽중은 1,0
월동기간 동안 zoysiagrass[Zoysia matrella(L.) Merr]와 creeping bentgrass(Agrostis palustris Hunds)의 저온 스트레스에 대한 탄수화물 대사 반응을 구명하기 위해 11월부터 이듬해 4월까지의 식물체내의 탄수화물 대사산물의 변화를 비교 분석하였다. Creeping bentgrass의 녹색도와 뿌리 성장은 온도변화와 거의 평행하게 변화하였으나 zoysiagrass는 뿌리 성장에 거의 변화가 없이
Zoysiagrass [Zoysia marella (L.) Merr.]와 creeping bentgrass (Agrostis palustris Huds)에서 고온 스트레스에 대한 탄수화물대사 반응을 규명하기 위해 4월부터 9월까지의 식물체내의 탄수화물 대사산물의 변화를 비교 분석하였다. 각 초종의 샘플은 1998년 조성된 무안 Country Club의 green과 fairway에서 채취하였다. Creeping bentgrass의 지상부 수량은 6월부터
To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. Th
In this study, we analyzed the relationships between weather factors and photosystem II activity (Fv/Fm), as a measure of photochemical efficiency, in three cool-season turfgrasses commonly planted on golf courses in Jeju, South Korea: perennial ryegrass (Lolium perenne L.), Kentucky bluegrass (Poa pratensis L.), and creeping bentgrass (Agrostis palustris Huds.). In all three turfgrasses, Fv/Fm was higher during late summer than during early summer. However, in late summer, Fv/Fm was significantly lower in perennial ryegrass than in the other two species. In early summer, Fv/Fm in perennial ryegrass and Kentucky bluegrass was positively correlated with mean low temperature and extreme minimum temperature, whereas, in late summer, this parameter in Kentucky bluegrass and creeping bentgrass was positively correlated with relative humidity, and in creeping bentgrass was negatively correlated with mean high temperature, mean low temperature, and extreme maximum temperature. These results indicate that raising low temperatures is favorable for perennial ryegrass and Kentucky bluegrass in early summer, whereas, in late summer, the lowering of high temperatures proves to be beneficial for creeping bentgrass, and raising relative humidity is conducive to the growth of Kentucky bluegrass and creeping bentgrass. These findings will contribute to improving the selection and management of turfgrasses on golf courses and sports fields.
It was previously pointed out that mutation is the ultimate source of variation. Adequate variation is needed for plant breeding if there is a limitation in natural genetic resources. When the ionizing radiation has been known to cause chromosomal and genomic alternations, it is widely used for inducing mutagenesis. The electron beam as an ionizing radiation is the principal physical mutagens that induces mutation and effectively used in plant breeding. Since dose-response relationships of electron beam in plant species are rarely known, we investigated the seed germination rate and early seedling growth of irradiated seeds of creeping bentgrass (Agrostis palustris Huds., cv Penn-A1) with various electron beam irradiating conditions (1, 1.3, 2 MeV at both 0.03 mA and 0.06 mA with dose of 100 Gy (Gray) and 0.03, 1, 1.3, 2 MeV at 0.03 mA with dose of 200 Gy, respectively) using electron accelerator at Korea Atomic Energy Research Institute. The growth parameters in terms of shoot length, primary root length, and secondary root length showed similar response between 0.06 / 1 (mA / MeV) at 100 Gy and 0.03 / 0.3 (mA / MeV) at 200 Gy. Bentgrass seed germination was mainly affected by the intensity of irradiated dose (Gray). Germination rate was lowered as the irradiated dose increased. On the other hand, early seedling growth was mainly governed not by the dose of radiation but by voltage.
The direct use of mutation is a valuable approach to generate variability in crops. The electron beam, one of the ionizing radiations, has been applied to evaluate its effect on seed germination and early seedling growth of creeping bentgrass (Agrostis palustris Huds., cv Penn-A1). The mature dry seeds were irradiated with various electron beam energies (0.3, 1.0, 1.3, and 2 MeV) and current levels (0.03 and 0.06 mA). Although large variability was existed within each dose, distinct difference of germiability and seedling vigor were not found at 0.3 MeV / 0.03 mA and 0.3 MeV / 0.06 mA beam condition. However, 1.0 MeV / 0.06 mA application most effectively inhibited and retarded seed germination and most severely restricted cotyledon and root growth in early seedling growth. The direct use of electron beam would be a valuable supplementary approach to generate mutants suitable for breeding purposes.