Daechung Reservoir has been suffering from severe cyanobacterial blooming periodically due to the water pollutants from the watershed, especially nutrients from nonpoint sources. As a countermeasure, an artificial wetland was constructed to mitigate the pollutant load from the watershed by utilizing the vegetation. We investigated the water quality of the influent and outflow of the wetland during years 2014~2020 to evaluate the performance of pollutant removal through the wetland. Major pollutants (e.g. BOD, COD, SS, T-N, and T-P) were largely reduced during the retention in the wetland while nutrients removal was more efficient than that of organic matters. Pollutant removal efficiency for different inflow concentrations was also investigated to estimate the wetland’s capability as a way of managing nonpoint sources. The efficiency of water treatment was significantly higher when inflow concentrations were above 75th percentile for all pollutant, implying the wetland can be applied to the pre-treatment of high pollution load including initial rainfall runoff. Furthermore, the yearly variation of removal efficiency for seven years was analyzed to better understand long-term trends in water treatment of the wetland. The annual treatment efficiency of T-P was very high in the early stages of vegetation growth with high concentration of inflow water. However, it was confirmed that the concentration of inflow water decreased, vegetation stabilized, and the treatment efficiency gradually decreased as the soil was saturated. The findings of the study suggest that artificial wetlands can be an effective method for controlling harmful algal blooms by alleviating pollutant load from the tributaries of Daechung Reservoir.

The present study aimed to analyze the metaproteome of the microbial community comprising harmful algal bloom (HAB) in the Daechung reservoir, Korea. HAB samples located at GPS coordinates of 36°29’N latitude and 127°28’E longitude were harvested in October 2013. Microscopic observation of the HAB samples revealed red signals that were presumably caused by the autofluorescence of chlorophyll and phycocyanin in viable cyanobacteria. Metaproteomic analysis was performed by a gelbased shotgun proteomic method. Protein identification was conducted through a two-step analysis including a forward search strategy (FSS) (random search with the National Center for Biotechnology Information (NCBI), Cyanobase, and Phytozome), and a subsequent reverse search strategy (RSS) (additional Cyanobase search with a decoy database). The total number of proteins identified by the two-step analysis (FSS and RSS) was 1.8-fold higher than that by one-step analysis (FSS only). A total of 194 proteins were assigned to 12 cyanobacterial species (99 mol%) and one green algae species (1 mol%). Among the species identified, the toxic microcystin-producing Microcystis aeruginosa NIES-843 (62.3%) species was the most dominant. The largest functional category was proteins belonging to the energy category (39%), followed by metabolism (15%), and translation (12%). This study will be a good reference for monitoring ecological variations at the meta-protein level of aquatic microalgae for understanding HAB.

하절기 수화발생이 빈번한 대청호에서 2003~2005년(3년)에 걸쳐 분자생태학적 방법의 하나인 DGGE를 이용하여 시간에 따른 미생물 군집구조의 변화를 연구하였다. 조사기간 동안 출현한 식물플랑크톤을 형태학적으로 분류한 결과 Cyanophyceae가 우점하였고, 이중에서 Microcystis, Planktothrix (Oscillatoria), Phormidium 그리고 Anabaena 속이 크게 우점하였다. 분자적 군집분석 방법으로서 16S rDNA

This survey was accomplished to compare the characteristics of water quality and algae at the Daechung Dam with those at its regulating reservoir from the year 2003 to 2005. In the case of Daechung Dam, three sites, such as the area near dam body, the Mooneui intake station, and Chudong intake station, were selected averaged data were used for the comparison. The water quality of the Daechung Dam are an follows; temperature is from 4 to 25℃(average 15), pH is from 7.3 to 8.6(average 7.9), DO is from 5.7 to 13.0mg/L(average 9.9), BOD is from 0.7 to 1.7mg/L(average 1.1), COD is from 2.5 to 4.4mg/L (average 3.1), SS is from 1.1 to 9.8mg/L(average 3.2), total coliform is from 0 to 37MPN/100mL(average 11), total phosphorus is from 0.008 to 0.074mg/L(average 0.022) and total nitrogen is from 1.087 to 2.175mg/L(average 1.492). The water quality of regulating reservoir are an follows; temperature is from 1.0 to 24.0℃(average 13), pH is from 6.7 to 8.2(average 7.6), DO is from 5.9 to 13.2mg/L(average 9.2), BOD is from 0.3 to 1.8mg/L(average 0.8), COD is from 2.4 to 5.3mg/L(average 3.8), SS is from 1.2 to 50mg/L(average 9.0), total coliform is from 0 to 260MPN/100mL(average 56), total phosphorus is from 0.005 to 0.224mg/L(average 0.04) and total nitrogen is from 1.123 to 5.227mg/L (average 2.3). The water quality of regulating reservoir had been the Class Ⅱ raw water criteria ein years ago, but it has been improved to Class I recently. Whereas the water quality of the Daechung Dam has been maintained as Ⅱ except COD.

2001, 2003년의 7월부터 10월까지 대청호에 위치한 댐 지역에서 식물플랑크톤 군집, 환경요인 및 강우량을 조사하였다. 조사 기간동안의 월평균 강우량은 2001, 2003년 각각 91.3, 265.3mm로 조사되었다. 최대 Chl. a의 농도는 2001, 2003년 각각 45.4, 131.5 ㎍ L/sup -1/로 조사되었다. 남조류 세포수는 2003년 최대 49,000 cells mL/sup -1/로 조사되었고, 2001년은 최대 200,000

본 연구는 1998년 5월부터 11월까지 대청호의 2개 정점 표ㆍ중ㆍ저층에서 수질과 일차생산을 나타내는 식물플랑크톤 현존량과의 관계를 규명하기 위하여 물리ㆍ화학적 환경요인(수온, 용존산소량, pH, 투명도, 총질소, 질산염, 아질산염, 암모니아, 총인, 인산염, 용존총인, 총유기탄소, 부유물질), 식물플랑크톤 현존량, chlorophyll-a의 농도를 조사하였다. 영양염류(아질산염, 질산염, 암모니아 및 인산염)가 과거에 비하여 증가되었으며, 총질소,

본 연구는 대청호에서 식물플랑크톤의 우점종 변화양상과 취기물질 생산종을 밝히기 위해 1998년 5월부터 11월까지 월별로 2개 정점(추동, 회남교)을 선정하여 이 화학적 수질과 식물플랑크톤의 분포를 조사하였으며, 대표적 취기물질인 geosmin과 2-methylisoborneol (MIB) 의 분포를 purge & trap concentrator가 장착된 gas chromatograph로 조사하였다. 조사기간에 나타난 식물 플랑크톤의 계절적 분포는 수온, pH 및 총질소/총인 등과 상관관계가 있는 것으로 분석되었다. 조사된 식물플랑크톤 중에서 cyanobacteria가 평균 54% (세포수 기준)의 우점을 나타낸 추동수역(정점 1)에서는 geosmin이 7 월, 8월 하순에 세포건량기준으로 각기 1.1 ng/mg, 18.1 ng/mg이 검출되었고, 63% 이상의 우점을 보인 회남교수 역(정점 2)에서는 geosmin이 7월, 8월 하순에 각각 0.7 ng/mg 및 69.8 ng/mg, MIB가 7월 하순에 0.6 ng/mg 검 출되었다. 따라서 geosmin과 MIB는 조사기간중 cyanobacteria가 우점하게 되는 7월, 8월에 주로 Anabaena에 의해 생산되는 것으로 판단된다.

더 많은 수자원을 확보하기 위한 하나의 대안으로서 다목적 저수지의 홍수조절공간과 이수공간 사이에서 용량 재할당 방안을 제안하였다. 저수용량 재할당이란 기존의 저수지에 물리적 변화 없이 좀 더 많은 편익을 얻을 수 있도록 상시만수위를 조정하는 관리 기법이다. 본 연구의 목적은 홍수조절에 미치는 악영향을 최소화하면서 용수공급에 필요한 용량을 증대시킬 수 있는 저수지 용량 재할당 방법론을 개발하는 것이다. 이 방법은 다양한 빈도별 유입홍수량에 대한 홍수기 저

이수목적 수량관리를 위한 보장수량과 수력발전 등을 고려한 최적화 기법은 이상가뭄 또는 특이 수문년을 포함하는 경우에는 적용에 한계가 있다. 이러한 관점에서 Hashimoto 등(1982)에 의해 제안된 물부족에 관련된 3가지 평가기준을 혼합정수계획법을 통해 최적화모형에 반영하기 위한 Moy 등(1986)과 Srinivasan 등(1999)에 의해 제시된 바 있다. 그러나 이들 연구는 용수공급용 단일 저수지에 대해 한정되어 발전을 포함하는 다목적댐에 적용