벗초파리(Drosophila suzukii)는 베리류 작물, 체리, 포도 등에 심각한 수확량 손실을 입히는 해충이다. 잘 알려진 훈증제인 phosphine (PH3)과 ethylformate (EF)를 저온과 병합처리 하면 벗초파리의 살충효과가 상승되는데 그 원인을 규명하고자 본 연구에서는 TUNEL assay 를 이용한 apoptosis 분석과 이차원 전기영동(2DE) 및 MALDI-TOF/TOF를 통한 벗초파리 체내 단백질 변화를 분석하였다. Apoptosis 분 석 결과, 저온과 훈증제 복합 처리구, 각각의 훈증제와 저온 단독 처리구 순으로 apoptosis가 강한 경향을 보였다. 단백질 발현 분석 결과, 약 800 개의 spot이 관찰되어 그 중 가장 변화가 큰 42개의 spot을 동정한 결과, cathepsin D과 heat shock protein 83이 훈증제와 저온 복합처리군에 서 발현이 현저히 증가했다. 이러한 결과는 훈증제와 저온 복합처리에 의한 벗초파리의 살충효과 상승작용에 대한 생리학적 변화를 확인하는 중 요한 지표가 될 수 있다.

Although ethylformate and phosphine fumigants are widely used for pest quarantine, studies related to their mechanism of action and metabolic physiological changes in Drosophila models are still unclear. In this study, we investigated how key metabolites altered by fumigants and cold treatment are associated with and affect insect physiology by comparative metabolome analysis. Fumigant treatment significantly altered cytochrome P450 and glutathione metabolites involved in the detoxification mechanism and showed lower expression of PGF2α involved in the immune response compared to the control. Additionally, most of the metabolites functioned in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors.

Drosophila suzukii (Diptera, Drosophilidae) can damage thin-skinned fruits (plums, cherries, peaches, blueberries, and strawberries) by laying eggs inside the fruit. In this study, a basic experiment was conducted to investigate the preference of D. suzukii to export table grape varieties. Four varieties of grapes (Kyoho grape, Shine muscat, Campbell grape, and Black sapphire) were placed in a cage containing more than 2,500 number of D. suzukii adults for 6, 8, 10, 12, and 24 hours and the egg-laying in grape has been induced. After 2 weeks, the number of pupae that emerged was counted. As a result, the largest number of pupae was generated in Kyoho grape among the four types of grapes, indicating that D. suzukii prefers to lay egg in Kyoho grape. Through this experiment, it was determined that 6 h was the appropriate inoculation time, and then an inoculation experiment was conducted on Kyoho grape. The total weight of the Kyoho grape used in the experiment was 36.119 kg, and a total of 2594 pupae were generated. The average number of D. suzukii per cluster was about 50. All stages of D. suzukii treated with low temperature(1℃) for 10 days were completely controlled.

벗초파리기생벌인 A. japonica의 벗초파리 유충의 발육단계에 따른 기생특성과 기생당한 유충과 번데기에서 형태적인 차이를 조사하였다. 또한 A. japonica의 우화기간이 벗초파리 우화일수 보다 더 소요되는 것을 확인하였다. A. japonica는 벗초파리 유충에 효과적인 기생 및 살충 효과를 나타내었으며 벗초파리 방제를 위한 천적으로 활용이 가능할 것으로 보인다.