In order to renovate spirodiclofen contaminated soil through biodegradation, a high-effective spirodiclofen degrading bacterium was isolated from corn cultivated soil in Weifang of Shandong, China using enrichment culture and HPLC method. The strain identification was studied, the best cultivated conditions and pesticides degrading substrate range of the strain was also determined. The spirodiclofen degradation rate of the screened strain WF14-6 was 76.42% after 120 h cultivation, based on morphology and 16S rRNA gene sequence analysis, the strain was identified as Enterobacter sp. The best cultivated temperature and pH of the strain were 30℃and 7.0. After 72 h cultivation, the degradation rate of strain to imidacloprid and acifluorfene was 31.35~41.06%, the degradation rate to bupirimate、diafenthiuron and dimethomorph was 20.81~23.90%.

오염된 인삼분말로부터 1 종의 세균을 순수분리하여 API kit 및 전자현미경을 이용하여 형태적, 생리적 특성을 조사하였다. 분리균은 직경 0.6-1.0㎛, 길이는 1.2-3.0㎛ 이었고 세포표면에 편모를 가진 간균이었다. 분리균은 β-galactosdase, arginine dihydrolase, ornithin decarboxylase를 가지고 있으며 탄소원으로 citrate를 이용할 수 있지만 H_2S는 생성하지 못하였다. 또한 glucose, manitol, sorbitol, rhamnose, sucrose, melibiose, arabinose 등의 당 및 amygdalin을 탄소원으로 이용할 수 있었다. 이상의 API kit를 이용한 생리적 특성분석 및 전자현미경을 이용한 미세형태적 특성 관찰결과에 의해 본 균주는 장내세균과(Enterobacteriaceae)에 속하는 Enterobacter sp로 동정되었다. 한편 분리균주 Enterobacter sp.의 생육에 미치는 인삼사포닌의 영향을 조사한 결과 사포닌은 농도의존적으로 균의 생육을 억제하는 경향을 나타내었다. 즉, 사포닌을 0.05, 0.5, 2.0, 4.0% 첨가하고 38℃에서 3일 동안 배양한 후 사포닌 비첨가군과 비교한 결과 균의 상대적 성장률은 각각 75.0, 37.5, 7.5, 0.5%로 나타났다. 사포닌의 미생물 생육 억제작용은 비교적 고농도인 4.0% 사포닌 첨가시에도 초기의 6.0×10^6에서 2.0×10^7 CFU/g으로 완만한 증가경향을 나타내어 사멸작용보다는 정균작용일 것으로 추측된다.

The bacterial strain JE-1 degrading and utilizing Congo Red as a sole carbon source was isolated from dye-contaminated soil and identified as Enterobacter species. Enterobacter sp. JE-1 had the highest decolorization ability when it was cultured in the medium containing 0.05% NH_4NO_3, 0.05% K_2HPO_4 0.03% MgSO_4·7H_2O, 0.025% Congo Red, initial pH 7.0 at 30℃, respectively. Enterobacter sp. JE-1 had the relatively high substrate specificity. The dye decolorizing activity was exclusively extracellular. The expected metabolic intermediates of Congo Red by Enterobacter sp. JE-1 were analyzed by GC/MS. As a result, metabolic products like hexadecanoic acid, 1,2,3-triphenylcyclopropene, aliphatic hydrocarbons such as hexadecane, heptadecane, octadecane, hexacosane etc., and 1,2-benzenedicarboxylic acid, dibutyl ester were detected. Benzidine did not detected.