메타바코딩을 이용한 환경 DNA 분석은 검출 감도가 높아 어류의 생물다양성 평가 및 멸종위기종의 검출에 유용 한 기술이다. 이번 연구는 메타바코딩을 이용해 우리나라 담수어류를 대상으로 높은 검출 효율을 보일 수 있는 적합 한 분석방법을 확인하기 위해 4가지 분석조건별, 즉 필터 (cellulose nitrate filter, glass fiber filter), 추출 키트 (DNeasy® Blood & Tissue Kit, DNeasy® PowerWater Kit), 프라이머 조합 (12S rDNA, 16S rDNA) 그리고 PCR 방법 (conventional PCR, touchdown PCR)로 나타나는 Operational Taxonomic Units (OTUs) 수와 종 조성을 비교하였다. Glass fiber filter와 DNeasy® Tissue & Blood Kit를 이용해 추출한 시료는 12S rDNA와 16S rDNA 프라이머 조합에서 담수어류 OTUs가 가장 많이 검출되었다. 모든 분석조건 중 프라이머 조합에서만 조기어강 (Class Actinopterygii) 평균 OTUs 수에서 통계적으로 유의한 차이를 보였고 (Non-parametric Wilcoxon Signed Ranks Test, p=0.005), 담수어류 평균 OTUs 수는 유의하지 않았다. 종 조성 비교 결과 역시 프라이머 조합에서 유의한 차이를 보였고 (PERMANOVA, Pseudo-F=6.9489, p=0.006), 나머지 분석조건에서는 유의한 차이를 보이지 않았다. NMDS 분석 결과 종 조성은 유사도 65% 기준에서 프라이머 조합에 따라 묶였고, 16S rDNA 프라이머 세트는 주로 멸종위기종인 모래주사 (Microphysogobio koreensis), 꼬치동자개 (Pseudogobio brevicorpus)가 기여하였고, 12S rDNA 프라이머 세트는 주로 일반종인 피라미 (Zacco platypus), 꺽지 (Coreoperca herzi) 등이 기여한 것으로 나타났다. 본 연구는 국내 하천에서 채취한 시료에 대한 메타바코딩을 이용한 종 다양성 분석의 기초정보를 제공한다.

국내 멸종위기 어류 25종의 먹이원을 문헌 조사한 결과, 먹이원은 20문, 31강, 58목, 116과, 154속으로 나타났다. 먹이원 중 가장 많은 어류가 섭식한 먹이원은 분류군에 따라 절지동물문, 곤충강, 파리목, 깔따구과로 조사되었으며, 식물류 먹이원은 돌말문, 윷돌말강, 반달돌말목, 반달돌말과로 조사되었다. 계층적 군집분석과 NMDS를 이용하여 멸종위기 어류 20종의 먹이원 유형화 결과, 어류를 주로 포식하는 충식성 어류와 식물플랑크톤을 섭식하는 어류 2가지 유형으로 나타났다. 네트워크 분석의 허브 점수가 높은 먹이원은 파리목, 하루살이목, 날도래목, 강도래목, 딱정벌 래목으로 나타났으며 식물류 먹이원 중 허브 점수가 높은 쪽배돌말목과 반달돌말목, 김발돌말목으로 조사되었다. 먹이원 폭이 큰 어류는 연준모치 (PP)와 열목어, 좀수수치, 가는돌고기, 꼬치동자개, 퉁사리, 묵잡자루, 미호종개로 Bi 지수 값이 0.3 이상으로 조사되어 다양한 먹이를 먹는 것으 로 조사되었다. 반면, 금강모치, 부안종개, 감돌고기, 흰수마자, 다묵장어, 돌상어, 얼룩새코미꾸리, 북방종개는 Bi 지수 값이 0.1 이하로 조사되어 먹이원 다양성이 낮게 조사되었다.

This study was conducted to elucidate microplastics detection of freshwater ecosystems in Geum river. Samples are collected at 6 points in freshwater, 5 points in fishes. Freshwater was sampled 100 L per each points and fish species were Opsariichthys uncirostris amurensis, Hemibarbus labeo, Pseudogobio esocinus, Zacco platypus, Micropterus salmoides and Cyprinus carpio. FTIR analyis was adopted to identify microplastic types. Extracted microplastics were PES (polyester), PE (polyethylene), PP (polypropylene), PET (polyethylene terephthalate), PVC (Polyvinyl chloride) in freshwater, and PES, PE, PP, PET, PVC in fishes. Our results were expected to be used basic research information for further study in microplastics of freshwater ecosystems.

Invasive non-native species are one of the main threats to biodiversity. Recently, more than 30 non-native species were reported to have established in the four major rivers of South Korea, and introduction of non-native species is gradually accelerating. However, ecological information necessary for prevention and management of non-native species introductions and consequent spread is lacking in South Korea, and especially so for freshwater non-native species. In this study, we assessed eight non-native freshwater fishes established in the Geum River watershed [the Risk Assessment (RA) area] using the Aquatic Species Invasiveness Screening Kit (AS-ISK). Receiver operating curve analysis identified threshold values of 24.75 for the BRA (Basic Risk Assessment) and 35.75 for the BRA+CCA (BRA + Climate Change Assessment). Four species, namely Carassius cuvieri, Lepomis macrochirus, Micropterus salmoides and Oreochromis niloticus were ranked as ‘high risk’; whereas, the lowest scoring non-native species was Coreoperca kawamebari, which is not likely to be invasive in the RA area because it has not established self-reproducing populations and its distribution is currently narrowing across the country. The results of this study indicate that application of AS-ISK allows the assessment of potentially invasive non-native species and provides a framework of action for the development of management guidelines for non-native species and the conservation of native species (cf. ecological traits such as species competition, habitat preference, food resources, and reproduction). As a case study for the Geum River watershed in South Korea, it is recommended that additional screening studies should be carried out for the non-native species in the four major rivers of South Korea.

A recent topic of interest within ecology is ecosystem services that are importantly connected with biodiversity. Biodiversity is a fundamental component of ecological studies and has been the subject of many studies worldwide. However, studies on the diversity and distribution of freshwater fish were not undertaken in earnest until 2000, and the number of internationally available studies is therefore still limited. In this study, we used data from “The Survey and Evaluation of Aquatic Ecosystem Health (hereafter SEAEH)” within the framework of which whole water systems on the Korean peninsula are annually monitored to investigate and determine the current status of the diversity and distribution of freshwater fish, as well as to elucidate major factors impacting freshwater fish. A total of 130 species from 28 families were identified at around 953 sites in the SEAEH investigation of 2014. The species Zacco platypus (relative abundance: 28.2%) and Z. koreanus (19.3%) were identified as the most abundant species on the Korean peninsula, and besides these 20 endangered species, 51 endemic species and 4 exotic species were also collected. Highest (96 species) and lowest (72 species) diversity were identified in the Han River Watershed and the Yeongsan/Seomjin River Watershed, respectively. The mean endemic rate was 32.7%, which is higher than the previous rate determined in 1995. The exotic species Micropterus salmoides, Lepomis macrochirus and Carassius cuvieri were found in the whole river system except north Yeongdong, whereas Oreochromis niloticus occurred only at three sites due to the cold water temperatures in winter. Totally, 28 species were found to be translocated to different water systems not connected with known original habitats, most translocations occurring in the Nakdong River and the Yeongdong region. Among the translocated species, Opsariichthys uncirostris amurensis, and Erythroculter erythropterus were dominantly distributed in the mid- to lower reaches of Nakdong River where they may be harmful to the native fish assemblages due to their strong predation. The construction of weirs in connection with the “4 River Project” generated changes in fish assemblages before, during and after the project, resulting in altered habitat condition. Especially, a decrease in the number of endemic species and an increase in the abundance of lentic species were seen. Human-induced global warming will allow exotic species requiring high water temperatures to adapt to the environmental conditions of the Korean peninsula, which may enhance the diversity of native fishes and create changes in distribution. For the establishment of realistic and efficient management and protection strategies for Korean freshwater fish, SEAEH, which produces fundamental and quantitative data for Korean streams, is an important and necessary tool.

1998년 9월 30일부터 10월 7일에 걸쳐서 강원도 동강 유역인 정선군 정선읍 귤암리, 정선읍 가수리, 신동읍 운치리, 신동읍 고성리, 신동읍 덕천리 등 5개 지역에서 투망으로 포획한 담수어 11종, 178마리를 대상으로 인공 소화액법을 이용하여 Metagonimus 속의 피낭유충에 의한 감염률을 조사하였다. 감염률은 퉁가리 (Liobagrus andersoni) 91.7% (l1/12), 돌상어 (Gobiobotia brevibarba) 75.0%

국립공원에서 자연보존의 일환으로 기초자료를 축적하고자 1993년 5월부터 11월까지 덕유산국립공원 일대의 9개 지소에서 담수어류상을 조사한 결과 한국고유종 및 아종인 칼납자루, 긴몰개, 돌마자, 금강모치, 참종개, 미유기, 동사리를 포함한 5개과 17종의 담수어류를 확인하였다. 금강수계에서는 16종이, 낙동강수계에서는 8종이 분포하였으며 공통적으로 분포하는 어종은 긴몰개, 돌마자, 돌고기, 버들치, 갈겨니, 미꾸리 및 동사리로서 7종이었고 우세종은 버들치, 갈겨니 그리고 동사리였다. 한편 한국산 담수어의 형태에 관한 기초자료 축적의 일환으로 이지역에서 채집된 담수어 17종의 척추골수를 soft X-ray를 활용하여 그 분석결과를 보고함과 아울러 수계의 환경훼손에 따른 어류의 비정상적 골격형질 발현양상 파악결과의 활용가능성을 검토하였다.

국립공원에서 자연보존의 일환으로 기초자료를 축적하기 위하여 1992년 5월부터 1992년 10월까지 소백산국립 공원 일대의 9개 지소에서 담수어류상을 조사하였다. 이 지역에는 13종의 한국고유종을 포함하여 9개 과에 속하는 27종의 담수어류가 분포하였다. 한강수계에서는 15종이 낙동강 수계에서는 17종이 분포하였고 공통적으로 분포하는 종은 6종뿐이었다. 이 지역의 우세종은 갈겨니, 피라미, 버들치 그리고 동사리였다. 갈겨니. 피라미, 버들치를 포함한 11종에서 형태적 이상을 나타낸 개체가 출현하였다, 따라서 이와 같은 양상에 대하여 구체적인 조사와 사려 깊은 대책이 절실히 요망된다.

The fish species collected in the Keumho River basin are 42 species 31 genera elonging to 15 Families. This report was investigated for the evaluation of water quality in the Keumho River system which is a tributary of Nakdong River in Korea on september in 1999. The fishes collected were 42 species, 31 genera belonging to 15 Families. The dominant species were 5 species; Zacco platypus, Zacco temmincki, Squalidus chankaensis tsuchigae, Moroco oxycephalus, Squalidus gracilis majimae, and 8 species; Hemibarbus longirostris, Pseudogobio esocinus, Culter brevicauda, Cobitis rotundicaudata, Pseudobagrus fulvidraco, Pungitius sinensis kaibarae, Monopterus albus, Clanna argus were rare species. The relationship among the GPI, EC and BOD by the organic pollutants were over 0.9. The group pollution index(GPI) was lowest at St. 1(0.85) and highest at St. 5(2.33). The water quality of the Keumho river divided into 3 parts; the water of upper reaches in river(St. 1) was 1st class(oligotrophic condition), middle parts(St. 2, 3, 4) were 3rd class( a -mesosaprobic condition) and lower part(St. 5) was 4th class(Polysaprobic condition) as the source of tap water, respectively. And the tributary which are the Sinryeong Stream(St. 6), the Sincheon Stream(St. 7) and the Donghwa Stream(St. 9) in Keumho river were 2nd class as the source of tap water. The results in this study was represented same patterns as the result by the use of indicator species like as algae and invertebrates for the discrimination of water quality. So, some freshwater fish species can be use applicant for the discrimination of water quality.