현재 주요 수목병해 방제는 육안조사 등을 기반으로 한 현장조사를 통해 실시 되고 있으나, 목적 달성에 있어 그 효율성 높지 않다. 수목병의 예찰 방법을 고도화하고 적기방제를 통해 그 피해를 저감시키기 위해, 본 연구는 메타지놈 분석을 이용하여 두릅나무에서의 곰팡이 다양성을 조사하였다. 속(Genus) 수준에서 메타지놈 분석결과 총 20개의 곰팡이 상이 해당 재배지 내 두릅나무에 분포하는 것으로 확인되었다. 그 중, Alternaria속과 Puccinia속이 우점하는 것으로 조사되었으며, 이는 두릅나무 재배지 내 전체에서 개체별로 일관되게 나타났으며, 특히 해당 재배지 내 두릅나무 녹병이 우점적으로 발생함이 확인되었다. 이러한 결과를 바탕으로 두릅나무에서의 주요 곰팡이 다양성에 대한 스크리닝과 함께 주요 또는 잠재적 수목병원균류를 대상으로 메타지놈 기법으로 확인할 수 있음을 본 연구결과를 통해 확인되었다.

Fungi have been known as an irritant or causal agent of asthma. Thus, information on their concentration and diversity in indoor air of asthmatic patient’s houses is valuable for the management of these micro-organisms. The present study was performed to investigate fungal concentration and diversity in indoor air of resident spaces in two adult patient’s houses in winter. Air samples were collected in February of 2018 from living rooms and bedrooms of two asthma patients’ houses located in two different places in Incheon city using an impaction method. The average concentrations of airborne fungi did not exceed the 500 CFU/m3 level which is recommended by the Enforcement Managements of the Indoor Air Quality, Ministry of Environment, Korea. A total of four genera and 23 species were identified based on morphological and molecular methods. Aspergillus and Penicillium were two major genera. Aspergillus pseudoglaucus and Penicillium citrinum were commonly found between the two patient’s houses. Among the identified fungi, three species, Aspergillus venenatus, Penicillium jamesonlandense, and Penicillium salami were found to be unrecorded species in Korea. The morphological and molecular characteristics of these three unrecorded species were described. Since these species produce spores well and a lot, they are considered to be a species that needs management. This study provides basic information on the fungi for indoor air quality management in the living spaces of asthma patients in winter in Korea.

Asthma is a chronic diseases that restricts the air passage ways and which is thought to be caused by several factors such as genetics, obesity, mVOC, air pollution, allergens, and fungi. Domestic information on fungi in the indoor air in the houses of asthma patients in the spring and fall is rarely available. Thus, this study was carried out to investigate fungal concentration and diversity. Air samples were collected from the houses of 6 asthma patient in April and October of 2017 located in Seoul and Gyeonggi Provinces using an impaction method. The average concentrations of suspended fungi in the indoor air at the houses of adult asthma patient in October exceeded the 500 CFU/m3 level recommended by the Enforcement Managements of the Indoor Air Quality Control Act. A total of 70 fungi were isolated and identified. Four genera and 11 species were from the April sampling and 10 genera and 27 species were from the October sampling. Among the identified fungi, five species, Arthrinium marii, Penicillium austrosinicum, Penicillium concavorugulosum, Penicillium exsudans and Penicillium johnkrugii were found to be unrecorded species in Korea. The known properties of all identified fungi and the morphological and molecular characteristics of the five unrecorded species were described.

본 연구는 표고 재배방식의 변화와 함께 톱밥재배가 증가됨에 따라 재배과정에서 발생 가능한 다양한 진균류를 분리, 동정하고 이들에 대한 다양성 분석하고자 시험을 수행하였다. 경남지역 5개 농가로부터 수집된 시료에서 405균주를 분리하고 이들에 ITS 염기서열 분석을 통해 24속 42종을 확인하였다. 분리된 진균들에 대한 동정결과 Penicillium 속이 11종, Trichoderma속 4종이 확인되어 높은 다양성을 보여주었다. T. harzianum 의 경우 5개 농가 에서 수집된 모든 시료에서 확인되었으며, Penicillium brevicompactum의 경우 3개 농가에 확인되었다. 2개 농가 에서 확인되어진 Scytalidium cuboideum의 경우 표고 톱밥배지 조기부후 및 버섯 발생 저해 등의 피해증상이 확 인됨에 따라 주의가 필요할 것으로 사료되었다. 또한 표고 톱밥재배의 경우 다양한 진균류이 존재가능하기 때문에 재배사 내부의 환경관리 및 예방이 필요할 것으로 판단되었다.

Total 24 endophytic fungal strains were purely isolated from Salicornia europaea L. native to representative saltern of the Korea, Gomso. Strains were partially identified using amplified internal transcribed spacer(ITS) regions and the phylogenetic tree were constructed. Furthermore, deduction of two diversity indices was done. Endophytic fungal isolates belonging to 9 genera, concretely in Aspergillus, Aureobasidium, Cladosporium, Fusarium, Gibberella, Macrophoma, Penicillium, Phoma, Stemphylium, and unidentified(Pleosporales), respectively. Especially, Aspergillus(29%), Penicillium(21%) and Fusarium(13%) were dominantly distributed genera from the roots of S. europaea. Among them, genera Aspergillus, Aureobasidium or Gibberella have been reported with their activities of plant growth promoting, induces systemic resistance in previous researches. This study is dealing with the securing the diversity of endophytic fungi that showing symbiotic relationship with their host halophyte S. europaea which take the counter stage as a new crop resource.

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 genera and 215 species. Among them, anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 general and 215 species. Among them , anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.