2019년부터 2023년까지 제주도와 전라남도에서 재배되는 바나나(banana, Musa acuminate × balbisiana)와 커피(coffee, Coffea arabica)에 발생하여 피해를 주는 해충을 조사하였다. 바나나에서 발생한 해충은 총 7종이 조사되었다. 차응애(Tetranychus kanzawai)는 대부분의 바나나 과 원에서 발견되었고, 매년 잎에서 고밀도로 발생하여 피해를 주었다. 귤가루깍지벌레(Planococcus citri), 담배거세미나방(Spodoptera litura), 차애모 무늬잎말이나방(Adoxophyes honmai) 순으로 돌발적으로 발생하여 주로 잎을 가해하였다. 과실을 가해하는 해충은 없었으나, 2022년도 제주도에 서 바나나 과실 표면에 흰색 실무늬나 녹슨 색으로 변색되는 피해를 유발하는 고사리그물총채벌레(Helionothrips femoralis)가 처음 발견되었고, 2023년에 전라남도 완도와 해남에 소재한 바나나 농가에서도 이 해충이 발생하는 것이 확인되었다. 커피에 발생한 해충은 총 5종이 조사되었다. 이중 탱자소리진딧물(Toxoptera aurantii), 귤가루깍지벌레, 차애모무늬잎말이나방순으로 매년 다량 발생하여 피해를 주었다. 전라남도 소재 농가 에서는 달팽이종류인 두줄민달팽이(Limax marginatus)가 일부 잎과 과실에 피해를 주기도 하였고, 미국선녀벌레(Metcalfa pruinosa)가 일부 나무 에서 발생하기도 하였다.

RNA interference (RNAi)-based strategy has been developed to control various phytophagous chewing pests. However, only a few cases of RNAi-based control success have been reported for sucking pests, suggesting that sucking pests likely ingest less amount of transgenic subcellular hairpin RNA (or dsRNA). In this study, as the basic information for the establishment of ingestion RNAi against sucking pests, feeding amount and time course of plant subcellular fractions of the four sucking pest species (Frankliniella occidentalis, Frankliniella intonsa, Tetranychus urticae and Nilaparvata lugans) were determined by quantitative PCR (qPCR). Adults of the four species were starved for 24 h and then fed with kidney bean leaf (F. occidentalis, F. intonsa, T. urticae) or rice leaf (N. lugens) for 48 h. The leaf-fed adults were collected every 6-h interval and their genomic DNA was extracted. The ingested fractions of chloroplast and nuclear were quantified using rubisco and 50s rRNA as marker genes, respectively. The ingested amount of rubisco and 50s rRNA genes in F. occidentalis, F. intonsa and T. urticae showed rapid increasing pattern after feeding and then slightly reduced over time. In contrast, N. lugens neither ingest nuclear nor showed any distinct feeding pattern of chloroplast. These results demonstrate that F. occidentalis, F. intonsa and T. urticae ingest both chloroplasts and nucleus along with cytosol as cell-feeders but N. lugens, a phloem sap feeder, does not ingest nucleus during sucking. Our findings further suggest that ingestion RNAi-based control strategy would work better for cell-feeding sucking pests compared to phloem sap-feeding sucking pests.

Sampling insect pest population is often a necessary component of a proposed pest control strategy. Insect populations are poikilothermal animal and readily applied to model systems in several ways. When insect population immigrate to new possible habitats, they should be related with biotic and abiotic environments for survive and settle down. Based on climate change scenario, invasive insect species would be overcome its geographical limitation as well as local distribution of well-known species should be changed in near future. In this study, Species distribution modeling of native and invasive species were developed, compared and discussed for developing sampling strategies for invasive insect pest populations.

Carbonyl sulfide is a chemical that is registered as a crop protection product in Australia due to microorganisms such as soil, plant roots, branches, fertilizers and compost. In order to develop new fumigants for domestic use, the susceptibility of Carbonyl sulfide fumigants to two agricultural pests was evaluated. The susceptibility of peach aphid and spotted mite, which is an agricultural pest, was evaluated. After 5 hours of treatment (10, 20, 40, 60, and 80 mg / L) . As a result, peach aphid larvae showed a high mortality rate of 97.6% at 10 mg / L and 100% at 20 mg / L or more. Larvae were found to have a rate of 95.8% at 80 mg / L. The insect mortality rate was 91.8% at 60 mg / L and 94.1% at 80 mg / L.

Global warming can seriously influence on the interaction between pest and natural enemy in the agroecosystem due to the differences in optimal temperature ranges. Two aphid-ladybug systems, Myzus persicae-Coccinella septempunctata (M-C) and Aphis gossypii-Coccinella septempunctata (A-C) in the pepper crop were simulated, respectively under four different temperature scenarios including crop development over 244 days with the assumption that the average temperature is higher by 1, 3, and 5 °C than that in Seoul in 2000. Temperature-dependent functions for each aphid-ladybug system were embedded in Rosenzweig-Macathur predator-prey model to explore their population dynamics, and then Dynamic Index was used to quantify the strengths of species interactions. The result shows that the predator-prey population cycles as well as species interactions are getting shorter and stronger in both systems as temperature increased. Especially, the excessively high temperature scenario in Aphis gossypii-Coccinella septempunctata system could result in the extreme species interaction. Therefore, the increasing temperature can facilitate the effectiveness of biological control because of growing crop plant development and much stronger species interaction, although there are increases of the frequency of pest occurrences.

아스파라거스(Asparagus officinalis)에서 발생하는 해충의 종류 및 피해를 구명하기 위하여 2007년부터 2010년까지 제주도에서 조사를 수행한 결과, 다음과 같이 총 5개목 16종의 해충이 관찰되었다: 파총채벌레(Thrips tabaci), 하와이총채벌레(Thrips hawaiiensis), 차애모무늬잎말이나방(Adoxophyes honmai), 담배거세미나방(Spodoptera litura), 파밤나방(Spodoptera exigua), 왕담배나방(Helicoverpa armigera), 도둑나방(Mamestra brassicae), 네눈쑥가지나방(Ascotis selenaria), 줄고운가지나방(Ectropis excellens), 선녀벌레(Geisha distinctissima), 목화진딧물(Aphis gossypii), 복숭아혹진딧물(Myzus persicae), 청동풍뎅이(Anomala albopilosa), 아스파라거스잎벌레(Crioceris quatuordecimpunctata), 달팽이(Acusta despecta sieboldiana), 작은뾰족민달팽이(Deroceras reticulatum). 그 중에서 파총채벌레는 아스파라거스 전 기간에 걸쳐 발생하여 피해를 주는 해충이었다. 아스파라거스잎벌레와 달팽이류는 봄 수확기에 아스파라거스 순을 가해하여 큰 피해를 주었다. 아스파라거스 입경기부터 생육기에는 진딧물과 나방류 해충이 발생하여 피해를 주었다. 나방류 해충 중에서 파밤나방, 담배거세미나방, 왕담배나방 등 밤나방과 해충의 피해가 많았다. 본 결과를 바탕으로 아스파라거스 재배에서 해충방제 전략에 대하여 고찰하였다.

Rapid identification of pest species found under quarantine is an important factor in preventing an economic loss of agricultural commodities. In this study, we analyzed RNA-Seq of the larvae of C. sasakii, G. molesta and G. dimorpha, which are serious pests in several fruits in Korea and are difficult to discriminate by species in their larval stage because of lack of a morphological character. To select immunological diagnostic markers, discriminating the larvae of C. sasakii from the G. molesta and G. dimorpha, RNA-Seq was performed for the larvae of the three insects. The 454 pyrosequencing generated 3,058-4,686 contigs for each three pest species, which assembled into 2,584-3,970 isotigs with average lengths of 829-1,244 bp. Functional annotation of the sequencing results generated 774 orthologs for the three pest species, and 12 isotigs were finally registered as candidate markers for species discrimination through bioinformatical screening, literature search, and gene expression study. The selected candidates include serine proteases, serpins, 27 kDa glycoprotein and storage protein with a constitutive gene expression in their larvae, pupae and adult stage.

본 연구에서는 팥의 꼬투리와 줄기를 가해하는 Ostrinia속 해충에 대해 종 동정 과정을 기술하였고, 사육하면서 관찰된 발육특성들을 보고 하였다. 수컷의 생식기는 3-lobed uncus 형태였으며, 가운뎃다리 종아리마디에는 털을 많이 갖는 것으로 나타났다. 미토콘드리아 cytochrome oxidase I (COI)과 II (COII) 유전자의 부분 염기서열은 콩줄기명나방(O. scapulalis), 큰섬들명나방(O. zaguliaevi), 큰조명나방(O. zealis bipatrialis)들에 대해 일본과 중국에서 보고된 서열들과 100% 일치를 보이는 종은 없었다. 기주식물 범위는 국내외 보고들 간에 일치하지 않았다. 암컷 성페로몬샘 추출물의 가스크로마토그래피 분석에서 큰섬들명나방과 큰조명나방의 성페로몬 성분인 (Z)-9-tetradecenyl acetate는 검출되지 않았다. 이상의 결과들을 종합하여 고려하였을 때, 본 연구의 팥 해충을 가해하는 곤충 종은 콩줄기명나방(O. scapulalis)일 것으로 추정되었다. 가을 야외에서 채집된 유충들을 야외조건에서 보관하였을 때, 이듬해 6월과 7월 사이에 성충들이 우화하였는데, 이 결과로부터 본 곤충 종은 말령 유충 단계에서 겨울휴면을 하는 것으로 추정되었다. 이외에 반합성 인공사료를 이용하여 실내 사육이 가능하였다.