코드린나방은 사과, 배, 복숭아 등 과실류를 비롯한 다양한 작물에 피해를 주는 해충으로써, 대한민국 식물방 역법상 금지해충에 속해있다. 검역실적으로는 2016년 6월에 1건, 2018년 6월에 2건이 모두 인천공항으로 수입된 우즈베키스탄산 양벚에서 검출되었다. 코드린나방은 국내 침입이 매우 우려되는 해충 중의 한 종으로써 한국, 일본, 대만을 제외한 거의 모든 온대지역에 분포하고 있다. 따라서 우리나라는 본 해충에 대한 정밀하고 지속적인 표적 예찰이 필수 불가결이다. 효과적인 예찰을 위한 페로몬 트랩 개발을 위해 다음 사항들을 고려할 수 있다. 우선 페로몬 성분비에 따른 유인력의 차이를 검정해 효과적인 성분비를 찾는 것이 중요한데, 그 방법으로는 GC-MS/MS 분석법을 통해 루어의 페로몬 구성비율을 분석한다. 이후 적합한 실험환경을 설정하고 페로몬 혼합 물을 적용한 여과지를 케이지에 달아 일정 시간 동안 여과지에 접촉한 횟수를 기록 후 통계분석을 통한 유의성 검정을 실시한다. 이어서 페로몬을 루어에 주입, 흡착시킨 후 페로몬 트랩을 현장에 설치해 포획된 수컷 성충 수를 조사해 효과적인 약량 및 루어형태 등을 선별하게 된다. 이를 통해 효과적인 코드린나방 트랩 개발을 위한 기초연구를 수행하고자 하며, 우리나라의 농업과 자연환경 보호에 기여하고자 한다.
솔알락명나방(Dioryctria abietella)의 유충은 잣나무 구과를 가해 하는데, 피해를 심하게 받은 구과 종자의 손실량은 20.3%이며, 잣나무에서 구과피해율이 평균 25.7%에 달하여(이성찬, 2014), 잣 생산에 막대한 영향을 미치고 있다. 잣나무에서 페로몬 트랩의 설치 높이에 따른 솔알락명나방의 유인 효과 조사를 위하여 고가작업차 를 이용하여 잣나무림 지상부, 수관하부, 수관중부, 수관상부에 페로몬 트랩을 설치하였다. 페로몬은 케이아이 피에서 구입 하여 사용하였으며 트랩은 델타트랩을 설치하였다. 4월 26일에 최초 설치를 시작하여 기상상황에 맞게 10~15일 간격으로 수거하여 유인된 개체수를 조사하였다. 5월 초순에 4개 개체가 채집되기 시작하였으며, 6월 하순에 136개체가 채집되어 최대 밀도가 채집되었으며, 이후 점차 밀도가 감소하다가 8월 부터 밀도가 다시 증가하였다가 8월 중순 이후부터 다소 감소하기 시작하였다. 6월 초순에는 채집개체가 전혀 없었는데 이는 5월 하순부터 잣나무 송화가루가 날리면서 끈끈이트랩을 오염시켜 끈끈이트랩의 접착력이 떨어진 것에 기인한 것 으로 판단되었다. 수고별 솔알락명나방 페로몬 트랩 유인효과는 채집된 개체의 99.0%가 수관 상부에서 채집되 었으며, 2개체만이 수관중부에서 채집되었다. 수관하부와 지상에 설치된 트랩에서는 전혀 채집이 되지 않아 페로몬 트랩의 설치 높이에 따라 유인 효과가 매우 다른 것으로 나타났다.
Machilus thunbergii (Laurales: Lauraceae, 후박나무) is planted as a street tree in Jeollanamdo and Jeju Island. Orthaga olivacea (Lepidoprate: Pyralidae, 제주집명나방) is known as a major pest of M. thunbergii. For managing O. olivacea by eco-friendly control method, the mating disruption would be expected. The sex pheromone of O. olivacea was identified as a mixture of (Z)-11-hexadecenyl acetate (Z11-16Ac), (Z)-11-hexadecenal (Z11-16Ald), and (Z)-11-hexadecenol (Z11-16OH) at a ratio of 100: 0.1:0.1 by Chinese research group. Before applying mating disruption, confirmation of its attraction in Korea was essential. The pheromone traps composed of Z11-16Ac alone and the blend of three components (100:0.1:0.1) were installed in Haenam, Jeollanamdo in 2021, and Jeju and Seogwipo, Jeju in 2022. In field attraction test, no O. olivacea was attracted to any trap. However, Mamesastra brassicae (Lepidoptera: Noctuidae), which was not intended, was caught in both years in the trap. Total of 19 in Haenam between September 23 and 30, total of 11 in Jeju between September 30 and October 28, and total of 108 in Seogwipo between September 15 and November 11 were caught to the trap lured with Z11-16Ac. While in the trap lured with the blend, total 1 and 6 were captured in Haenam and Seogwipo, respectively.
The attraction effects of light emitting diode (LED) trap to Spodoptera exigua and Spodoptera litura adults were evaluated in greenhouse and compared with those of no light trap, which is typical used in commercial trap. At this time, in order to attract these two species of moths, sex pheromone traps were installed at the top side according to the degree of tomato growth inside the tomato cultivation greenhouse around the LED trap. In addition, two types of light-emitting traps (420 nm, 470 nm) were installed in the greenhouse at 1/40 m2, respectively. Also two sex pheromone were installed inside of the greenhouse according to the height of the tomato plants. 10 days later, Blue-light trap(BLB, 470 nm wavelength) was 3.1-3.5 times more attractive than Violet-light trap(VLB, 420 nm wavelength) in S. exigua (105.6 ± 7.3) and S. litura (42.0 ± 3.1) respectively, whereas the no-light trap was little attractive to S. exigua (33.7 ± 2.8) and S. litura (12.0 ± 1.5). On the other hand, after the installation of the sex pheromone trap and the LED trap, there was no damage to S. litura (Fabricius) and S. exigua in the pesticide-free area, indicating a high possibility of control. At this time, the operating cost of the two types of LED traps was 80 won/m2 per unit area, and it was confirmed that both types of moths could be controlled. In addition, as a result of confirming the number of two types of moths caught in the sexual pheromone trap and two types of LED traps after 4 months, it was judged that eco-friendly control was possible as more than 373 moths/trap were attracted to the two types of moths.
솔껍질깍지벌레는 남부지방의 곰솔을 가해하는 주요 해충으로 최근 그 분포범위가 확산되고 있다. 현재 솔껍질깍지 벌레의 모니터링은 난낭의 조사를 통해 이뤄지고 있으나, 이는 매우 많은 인력이 필요하며 부정확하다. 따라서 페로몬을 이용한 솔껍질깍지벌레의 모니터링이 새로운 대안으로 제시되고 있다. 하지만 이 역시 적절한 가이드라인이 없어 현장적용에 큰 어려움이 있다. 본 연구 에서는 페로몬을 이용한 솔껍질깍지벌레의 효율적인 모니터링 기법 개발을 위하여 최적 페로몬 방출농도 및 트랩설치 방법을 규명하였으며, 페로몬의 새로운 합성기법의 개발 통하여 경제적 효율성을 기하였다. 성 페로몬에 대한 수컷 솔껍질깍지벌레의 유인효과는 100 μg부터 1,600 μg 까지의 처리에서 용량의존적인 관계를 나타내었으며 이 이상으로 처리할 경우 페로몬 처리 증가에 따른 유인개체수가 증가하지 않았다. 트랩의 설치 높이를 지표면으로부터 50cm, 100cm 150 cm로 구분하여 조사한 결과 지상부로부터 50cm에 설치한 트랩에 가장 많은 개체가 포획되었다. 새로운 방법으로 합성한 페로몬 역시 기존 페로몬과 마찬가지로 페로몬 처리량과 유인 개체수 간 용량의존적인 관계가 나타났다.
Matsucoccus thunbergianae is the main forest insect pest of the Japanese Black Pine (Pinus thunbergii) in the west, south and east coast of South Korea. The main damage pattern appears to be the cumulative damage over many years by the second instar fixed under the pine bark. The adult male has a pair of wings and is attracted sex pheromone emanating from the adult female so that during the mating period (Generally, middle period of March) adult male is attracted to the adult female which has no wing. Using this mating behavior from the adult male, we developed sex pheromone trap to reduce mating success rate by reducing the density of the adult male in the field. In this study, In the indoor induction experiments and the field experiments, sex pheromone trap showed high induction effect on the adult male. Thus, we expect to reduce the damage to the Japanese Black Pine by Matsucoccus thunbergianae.
Insect pests on cruciferous crops and their natural enemies were surveyed during 2009~2011 in Daegwallyeong highland region which has been famous for summer Kimchi cabbage production in Korea since 1970s. Totally 15 insect pests have been reported in cabbage field in Daegwallyeong. Diamondback moth (DBM, Plutella xylostella) imported cabbage worm (Artogeia rapae L.), cabbage armyworm (CAW, Mamestra brassicae L.), cabbage looper (CL, Trichoplusia ni), cabbage sawfly (Athalia rosae ruficornis Jakovlev), and turnip aphid (Liphapis pseudobrassicae (Davis)) were the dominant pest species among the 15 species. For monitoring as well as controlling insects with weak flight, yellow sticky cards (10×15㎝) were used in cabbage fields. The sticky cards were hanged on plastic stick and placed at a 2-5 m distance within cabbage field (1,000㎡). Sex pheromone traps were employed for monitoring and controlling insects with strong flight. The survey result showed that there was significantly reduced pest occurrences in treated, compared to untreated condition. Similarly, The results from the sex pheromone traps were coincident with those of sticky trap. DBM, CAW and CL were found less in the treated than untreated fields; by 67.5%, 70.6% and 44.0%, respectively. Although the control efficacy of such traps was less than that of chemical spray, the use of sticky card trap combined with sex pheromone trap could be useful management tools for controlling insect pests in cabbage fields.
A male specimen of Lymantria albescens (called as Okinawa gypsy moth) was captured in Busan, by sex-pheromone trap for Asian Gypsy Moth (AGM) (7R,8S)-cis-7,8-epoxy-2- methyloctadecane [(+)-disparlure]. Up to now, this species is distributed only in Ryukyu Islands of Japan including Ishigaki and Okinawa. The male of Okinawa gypsy moth might be attracted to AGM pheromone trap. If L. albescens is occurred in Korea, more many male individuals must be captured in pheromone trap. Therefore, we considered that the individual might be imported from Japan by inanimate pathway. Although it is high probability that L. albescens might be imported from Okinawa, it is important to a survey on an invasive pathway of the species in a view point of quarantine inspection. Through this presentation, we provided a detection method on Lymantria species using DNA barcoding. On the basis of this study, we will conduct on an invasive pathway and inhabitation possibility.
Riptortus pedestris is an important soybean pest in Korea and Japan. Aggregation pheromone traps were evaluated in 12 soybean fields in Andong using three treatments of trap installation, i.e., installation for whole cultivation period, installation up to September, and no installation. The number of R. pedestris (in all life stages) were not significantly different among the treatments until October, but its abundance significantly increased at mid October in the fields where traps were installed. Trap catch of R. pedestris was relatively higher in August while bug population was low in the fields. However, in fall, R. pedestris populations become abundant in the fields while trap catch was low. There was no significant difference in parasitism as well as seed and pod damage among the treatments. In conclusion, aggregation pheromone trap should’nt be used as control measure, but can be used as a monitoring tool of R. pedestris population up to August in soybean field.