Molecular markers were crucial role in understanding and using genomic information for marker-assisted breeding, mapping genes of interest and cloning genes. Among the molecular markers, Simple Sequence Repeat (SSR) has became increasingly important marker due to their co-dominant and high polymorphic nature and abundant distribution throughout the genome. Simple sequence repeats (SSR), also called “microsatellites” consist of tandemly repeated short DNA sequence motifs and have applied in various marker-based studies. SSRs were isolated and characterized from “Heuktari” and “Miso”, which are one of the major oyster mushroom cultivars in Korea by the genome sequencing and bioinformatic analysis. The genome sizes of “Heuktari” were 40.8 Mb and “Miso” were estimated to be 40.3 Mb, which were larger than the other P.ostreatus species (PC9 and PC10) and less than P.eryngii (KNR2312 P5). A total of 949 and 968 SSRs were found in the “Heuktari” and “Miso” genomes, respectively. A comparative analysis of five mushrooms including P.ostreatus var. florida (PC9 and PC15), P.eryngii, revealed that SSR numbers from “Heuktari” and “Miso” were the highest among them. Studied all mushrooms showed similar pattern of SSR distributions. Tri-, hexa- and octanucleotide motifs accounted for the top three fractions of all SSRs. This study are useful in understanding the P.ostreatus.[Supported by a grant from the IPET (213007-05-1-SBI30), MIFAFF, Republic of Korea.]

Simple sequence repeats (SSR), also referred to “microsatellites” consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from ‘Heuktari’ and ‘Miso’, which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of ‘Heuktari’ and ‘Miso’ were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the ‘Heuktari’ and ‘Miso’ genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in ‘Heuktari’ and ‘Miso’ were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

his study was carried out to investigate antimicrobial activities of water extracted Pleurotus ostreatus var Heuktari (PO), and their application to F. fujikuroi Nirenberg growth inhibition material. Various organic solvents (chloroform, hexane, ethyl acetate, ethanol) were tested to investigate the antimicrobial activities of Pleurotus ostreatus var Heuktari against F. fujikuroi Nirenberg. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but butanol extract showed 2.5% strong activities in order of F. fujikuroi Nirenberg disk diffusion test. Then we observe Antifungal activity using green house. Bed soil and mushroom extract mixed at 10%, 7.5%, 5%, 2.5%, 1% total bed soil mass. Screening of Antifungal activity was tested two periode 18day and 25day. The Antifungal activity rate of each period and extract density was 7.5% mixed bedsoil has investigated 80% Antifungal activity rate. The result indicates that the Pleurotus ostreatus var Heuktari extract using F. fujikuroi Nirenberg registance, which shows the development of organic agriculture seed disinfectant.