Genetic modification of the pig of which the gene is relevant to human diseases allows the pig to be used as a source of biomedical animal model. The promoter which could drive efficient expression constitutively or specifically of the interest gene in porcine organs is essentially required to increase versatility of a biomedical porcine model. In this study, we compared different promoters of activities driving efficient expression in different types of porcine cells including primary fibroblasts, kidney-derived PK-15, and primary endothelial cells (EC). To this end, we inserted CMV, EF1-α, CMV/EF1-α, CAG, human and porcine membrane cofactor protein gene promoters(MCP and Mcp), and porcine intercellular adhesion molecule-2 (Icam-2) promoter into pGL3 basic vector. Luciferase assay revealed that CAG promoter led to highest promoter activity in fibroblasts and PK-15 cells. CMV, EF1-α, CMV/EF1-α promoters showed moderate activities for luciferase expression in fibroblasts and PK-15 cells. Interestingly, CMV/EF1-α promoter, in which CMV promoter was linked to the front of EF1-α promoter as an enhancer led to highest luciferase expression in EC. The MCP, Mcp and Icam-2 promoters showed very low level of luciferase expression in three types of cells. Taken together, this study demonstrated that promoter activity in different porcine cells is differently expressed.

An endoparasitoid wasp, Cotesia plutellae parasitized young larvae of diamondback moth, Plutella xylostella. Parasitized larvae exhibit sign ificant immunosuppression and fail to metamorphose to pupal stage. Especially, during last instar of parasitized P.xylostella, massive nutrients divert from host to wasp development. CpBV15α ,a host translation inhibitory factors encoded in C. Plutella bracovirus(CpBV), plays a crucial role in suppressing host usage of amino acids. Its promoter analysis shows that CpBV15α specifically inhibit host development in late larval period. To understand its inhibitory target, its specific expression was performed in non-parasitized P. xylostella by in vivo transient expression technique. Total plasma proteins were analyzed by 2D gel electrophoresis and determined target genes inhibited by CpBV15α. Immunoprecipation of cellular extract with CpBV15α antibody captured eIF2B. CpBV15α shares sequence homology with eIF5, especially at its eIF2B-binding region. Our results suggest that CpBV15α may sequester eIF2B, which result in malfunctioning of eIF2 cycling to form a translation initiation complex.

본 연구에서는 물리, 화학, 생물적 처리과정 중 서로 다른 용량의 퇴비가 농촌쓰레기처리공정에서의 수치를 검측하였다. 그 결과, 농촌쓰레기의 5‰ EM 해결시, 지렁이의 생장에 유익했고 34일이 지나면 예비 비료화가 되고, 35일에는 벌레가 자랐다. 5‰ EM 처리 샘플의 C/N비, NH4+-N농도, 활성화 우레아제, 섬유소와 삭카라아제는 다른 그룹보다 낮았지만 NO3-N 농도는 다른 그룹보다 선명하게 높았다.

For stable germline transformation, the promoter of B. mori cytoplasmic actin gene (BmA3) was used to ubiquitous expression of transgenes. Except for BmA3 promoter, promoters used to regulate gene expressionin all tissues and developmental stages of B. mori were not nearly developed. To identify more powerful promoter than previously reported BmA3 promoter (Mange et al., 1997), we introduced a new dot blot hybridization method, and isolated nine clones that show stronger dot signal compared to the control, BmA3by this method. Among these 9 clones, we focused on one clone which has high amino acid homology (94%) with heat shock protein 70 gene of Trichoplusia ni. This resulting positive clone, named bHsp70 (B. mori heat shock protein 70) was ubiquitiously expressed in tissues and developmental stage of fifth instar B. mori larvae,and stimulated bythermal and ER stress. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1003/+147) in the 5'-flanking region of bHsp70 gene that has 264-fold more intensive promoter activity than BmA3 promoter. Moreover, transcription activity of bHsp70 promoter under heat shock condition (42 ℃, 4 hr) was increased over 2-fold than normal condition. Therefore, we suggest that bHsp70 promoter may be used more effective candidate for transgene expression in B. mori.

최근 곤충자원은 약용 및 식용으로 활용하려고 많은 연구가 되고 있으며 미래의 자원으로 발전할 수 있는 가능성은 충분하다. 본 연구는 곤충자원 중 칠성무당벌레와 무당벌레를 이용하여 생물학적 활성과 함께 약용 및 식용으로의 활용가능성을 알아보고자 하였다. 생물학적 활성을 알아보기 위하여 이들 추출물을 이용하여 항산화관련 실험인 DPPH, FRAP, linoleic acid 산화 억제실험을 하였으며, 분자 염증에 관련된 유전자인 Cyclooxygena

유선에서 젖의 생산은 뇌하수체 호르몬인 성장 호르몬과 프롤락틴을 포함한 여러 가지 호르몬의 조절을 받는다. 최근의 연구에 따르면 이 호르몬들 중에서 성장호르몬과 프롤락틴은 유선에서도 그 유전자 전사체가 발견된다 본 연구에서는 유선에서 발현되는 성장호르몬이 유선 특이 발현 유전자의 발현에 미치는 영향을 조사하고자 유선 특이 발현 유전자인 베타-락토글로불린(-lactoglobulin :BLG)의 프로모터를 모델 시스템으로 하여 소와 사람의 성장 호르몬이 유