본 연구에서 상동나무 가지 추출물(STB-E100)은 대장암 세포에서 세포사멸을 유도하여 세포생육을 억제하였다. 또한 Iκ B-α 인산화를 통한 IκB-α 단백질 분해를 유도하며 이로 인해 P65 핵내 전이를 유도하여 NF-κB 신호전달을 활성화시킨다. NF-κB 신호전달 활성화는 GSK3β 활성화를 통해 P65 핵내 전 이를 유도에 의한 것이지만 IκB-α분해는 GSK3β 의존성이 아니다. 상동나무 가지 추출물은 이러한 신호전달 활성화를 통해 세포사멸을 유도하여 대장암의 세포생육을 억제한다. 본 결과를 바탕으로 상동나무 가지가 암 예방 및 치료를 목적으로한 표적 요법에서 항암제 개발의 잠재적 활용 소재로서 이용 가능하다고 사료된다. 그러나 대장암 세포에서 상동나무 가지 추출물에 의해 유도된 NF-κB 신호전달 작용기전을 좀 더 구체적으로 구명할 필요가 있고 대장암에 대한 세포사멸과 작용기전의 정확한 관련성을 조사하기 위해 추가적인 연구가 필요하다.

In this study, we evaluated the effect of branch (STB) and leave (STL) extracts from Sageretia thea on β-catenin level in human colorecal cancer cells, SW480 and lung cancer cells, A549. STB and STL dose-dependently suppressed the growth of SW480 and A549 cells. STB and STL decreased β-catenin level in both protein and mRNA level. MG132 decreased the downregulation of β-catenin protein level induced by STB and STL. However, the inhibition of GSK3β by LiCl or ROS scavenging by NAC did not block the reduction of β-catenin protein by STB and STL. Our results suggested that STB and STL may downregulate β-catenin protein level independent on GSK3β and ROS. Based on these findings, STB and STL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer and lung cancer.

This study had been conducted to investigate the effect of some plant growth regulators inducing fruit enlargement and optimal harvest time in Sageretia thea. Two hundred fifty ㎎/ℓ mepiquat chloride treatment, 1㎎/ℓ thidiazuron treatment on full bloom, and 200㎎/ℓ gibberellic acid treatment on 7 days before full bloom resulted in the increase of 21.7% in weight, and 200㎎/ℓ gibberellic acid treatment 7 days before full bloom, 10㎎/ℓ forchlorfenuron treatment 14 days after full bloom, and 1㎎/ℓ thidiazuron treatment on full bloom also brought about positive effects on the enlargement of the fruit, increasing 6.3%, 6.3% and 8.1% in its transverse diameter, respectively. Furthermore, the effects of the plant growth regulator treatments on the harvest time of Sageretia thea were determined as follows: the increase in the optimal harvest time of 57.2 - 75.4%, shorter maturation period, by the treatments with 500㎎/ℓ mepiquat chloride 7 days after full bloom, 100㎎/ℓ gibberellic acid treatment on full bloom, 2.5㎎/ℓ forchlorofenuron 7 days after full bloom and 2㎎/ℓ thidiazuron treatment 7 days before full bloom; and the greater effects of plant growth regulator treatments on the fruit maturation in the following order, gibberellic acid > thidiazuron > forchlorofenuron > mepiquat chloride. The results of this study are expected to be used as a reference data to develop Sageretia thea as a new local specific crop for Jeju island.

Characteristic of seed for Sageretia thea fruits collected from the habitat of harvest season in Jeju and theirgermination ratio in different temperatures were considered. The average weight was 0.2g, average diameter was 7.2㎜,average length was 6.5㎜, and size distribution range was 5.1~10.0mm. The number of seeds per fruit was 1.8, and 1000grain weight was 7.77g with diameter of 3.7㎜ and thickness of 1.7㎜ in size. The fruit maturation was investigated to befrom April 27 to June 1, when the best maturation period was about a week, May 11 to May 18. Also, the fruits grown onMay 4 to May 11 were seen to have the bigger in transverse size and weight with a tendency of the earlier maturation datehas the greater the number of seeds. S. thea seeds pretreated for 24 hours at 50℃ were not germinated, while most of thosepretreated for 24 hours at 4℃ were successfully germinated at any degree of temperature (except at a temperature of 10℃).Particularly, the highest germination rate of 55% was made at 15℃, and plumule and radicle were best grown within thetemperature range of 25℃.

This study was carried out to investigate the vegetation and habitat environment of Sageretia thea which isdistributed in Jeju island, Korea. Sageretia thea were mainly distributed to the west area in Jeju island. Soil pH andEC of Sageretia thea habitat were 5.8 and 0.34dS•m−1. The contents of organic matter, available phosphate, exchangeablepotassium, exchangeable sodium, exchangeable magnesium and exchangeable calcium were 15.27%, 13.6㎎㎏−1,0.27cmol+㎏−1, 1.3cmol+㎏−1, 1.7cmol+㎏−1, 4.9cmol+㎏−1, respectively. Thirty one taxa including 25 families, 31 genus, 27species and 4 varieties were identified surrounding Sageretia thea habitat. Specific plant species were 1 taxon in III grade, 1taxon in I grade and 3 taxa in I grade.