검색결과

검색조건
좁혀보기
검색필터
결과 내 재검색

간행물

    분야

      발행연도

      -

        검색결과 3

        1.
        2024.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Salmonella enterica는 세계적으로 가장 널리 알려진 식 중독균이다. Salmonella enterica Enteritidis MFDS1022168 은 인천보건환경연구원에서 계란에서 분리된 균주로, 이 균 주가 지니고 있는 병원성에 대한 genome 수준의 메커니즘을 이해하기 위하여 whole genome sequencing을 진행 하여 유전체를 분석하였다. PacBio Sequel 장비를 이용하 여 유전체염기서열 분석을 진행한 결과 invA를 비롯한 여 러 종류의 병원성유전자를 갖고 있는 것으로 확인됐다. 이 에 따라 해당 균주의 독성을 파악하기 위해 유전체분석을 진행하였다. 유전자 총 수 예측 결과, 4,776개의 CDSs, 84 개의 tRNA, 22개의 rRNA를 보유한 것으로 나타났다.
        3,000원
        2.
        2008.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In order to investigate phenotype and genotype of Salmonella enterica subsp. enterica serovar Enteritidis, Forty-eight S. Enteritidis isolates from diarrhea patients were analysed using antimicrobial resistance typing, Phage typing, and Pulsed field gel electrophoresis in Seoul from 2004 to 2005. All of S. Enteritidis were resistant to streptomycin(SM, 37.5%), ampicillin(AM, 43.8%), t icarcillin(TIC, 43.8%), chloramphenicol(CM, 29.2%), t etracycline(TE, 10 .4%) and nalidixic acid(NA, 18.8%) among 16 antimicrobial drugs. Of 48 S. Enteritidis, 8 isolates(16.7%) were resistant to 1 drug, 3 isolates( 6.3%) to 2 drugs, 1 isolate (2.1%) to 3 drugs and 17 isolates(35.7%) to 4 drugs. The basic pattern of 4 drugs resistance was SM, TIC, TE, and CM but 1 drug resistant isolates represent all nalidixic acid resistance. Among 30 antibiotic r esistant S . Enteritidis, 2 1 isolates(70 %) were phage type 2 1, 8 i solates(26.7%) were phage t ype 2 3 and 1 isolate( 3.3%) was RDNC, respectively. Of the phage types observed, all of phage type 23(8 isolates) were nalidixic acid resistant and phage type 21 were AM-TIC-SM-CM multi-resistance(13 isolates; 43.3%), AM-TIC-SM-TE(4 isolates; 13.3%), AM-TIC-SM(1 isolate; 3.3%), AM-TIC-CM(1 isolate; 3.3%), and AM-TIC(2 isolates; 6.7%) resistance and 1 isolate of RDNC was NA-TE resistance. PFGE divided the isolates into two major clusters, A(n=14) and B(n=14). There were four different resistance profiles with resistance to AM, TIC, SM, TE, NA within PFGE A. Also resistance to AM, TIC, SM, CM was common within PFGE B. The PFGE A strains typed as PT21(n=5), PT23(n=8), and RDNC(n=1), While all the PFGE B strains typed as PT21(n=14). In consequence, there was the highly significant concurrence between resistance typing, phage typing and PFGE.
        4,000원
        3.
        2008.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In order to investigate the resistance patterns and molecular characteristics of resistance gene of 9 NA-resistant Salmonella enterica subsp. enterica serovar Enteritidis, Total of 101 isolates were isolated from stool sampes from 2005 to 2006. Among them, 48 isolates(47.5%) were identified S. Enteritidis, 24 isolates(23.8%) S. Typhi, 8 isolates(42.5%) S. Typhimurium, 7 isolates(6.9%) S. Paratyphi A and 14 isolates (13.9%) others Salmonellas. All of S. Enteritidis were resistant to ampicillin(43.8%), ticarcillin(43.8%), streptomycin(37.5%), chloramphenicol(29.2%), tetracycline(10.4%) and nalidixic acid(18.8%), respectively. All nalidixic acid-resistance isolates represented one point mutation in the quinolone resistance determining region(QRDR) of gyrA gene. Among them, 8(89%) isolates were substituted Tyr for Ser at position amino acid 83th or 1(11%) isolate was substituted Asn for Asp at position amino acid 87th. In consequence, Continued surveillance of NA-resistance among non-Typhi S. entetica isolates is needed to mitigate the increasing prevalence of nalidixic acid resistance.
        4,000원