Soybean seed injury was analyzed in the experiments in which the bean bug, Riptortus pedestris(Hemiptera: Alydidae), was released into screen-caged pots containing full seed stage(R6) of soybean. When the different stages of insects, from the 3rd instar nymphs to adults, were released into pots during 8 days in soybean R6 stage, soybean seeds with injury marks (B-type seeds) increased. The weight reduction ratio in B-type seeds was highest in the injury by the 5th instar nymphs, while the daily-produced number ratio of B-type seeds was small in the treatment. In the injury by the different number of adult released into pots, 4, 8, 16 adults caused a significant increase of B-type seeds, while 16 adults caused the significant increase of the deformed (C-type) seeds. In the injury by the different release period of adults, the total number of pods was not significantly different among treatments, while the total seed number harvested was significantly small in the release for 48 days. The release for 8 and 16 days caused a significant increase of B-type seeds, while the release for 48 days caused the increase of C-type seeds. The results indicated that injury of soybean R6 stage by the bean bug produced soybean seeds with distinct injury marks at relatively low density and during short term attack period, while it produced deformed seeds at high density and during long attack period.

콩 생식생장 단계별로 톱다리개미허리노린재를 접종하거나, 침으로 꼬투리를 치르는 인위적 가해를 통해 콩 종자 피해를 조사하였다. 착협기에 톱다리개미허리노리재 성충을 섭식시켰을 때, 빈 꼬투리와 종자로 전혀 발육하지 못한 판형종자의 생성비율이 종실비대성기에서의 가해보다 높은 것이 관찰되었다. 종실비대성기에 섭식시켰을 때는 종자모양은 거의 정상적이나 종피에 피해흔적이 뚜렷한 종자 수가 유의적으로 증가하였다. 종자모양이 많이 변형되거나 불완전하게 성숙한 기형종자의 비율은 처리 사이에 차이가 없었고 다른 종자형태들에 비해 상대적으로 낮은 비율을 차지하였다 착협시 피해에서 생성된 판 종자의 83%, 종실비대성기에 생성된 피해흔적이 있는 종자의 91%에서 종자 표면 위에 실험곤충의 빨대피막이 관찰되었다. 개화기 섭식은 피해종자 생성에 거의 영향을 주지않았다. 종실비대성기와 성숙기에 침을 이용하여 콩 꼬투리를 찔러 물리적인 가해를 하였을 때, 수확기에 건전한 종자들을 발견할 수 없었고, 피해gms적이 있는 종자의 비율이 기형종자 보다 많았다. 종실비대성기에 침으로 찌른 회수가 많은 경우 판형종자와 기형종자의 비율이 증가하였고, 피해흔적 종자 비율은 상대적으로 감소하였는데 무게감소 비율은 적게 찌른 경우 보다 상대적으로 켰다. 그러나 성숙기 인위적 가해에서는 수확종자의 무게감소가 나타나지 않았다.

Background : Oxidative stress-related iflammatory mechanisms may play an important role in the pathogenesis of cisplatin-induced nephrotoxicity. Safflower (Carthamus tinctorius L.) seed is a crude drug rich in serotonin derivatives to exhibit several biological activities, including antioxidant, anti-inflammation and anti-cancer effects. The purpose of this study was to determine the renoprotective effects of Safflower seed, using cisplatin-induced nephrotoxicity mice. Methods and Results : Safflower seed was orally administered at a dose of 100 and 200 mg/kg body weight for 5 days before the intraperitoneal injection of cisplatin 20 mg/kg body weight, and the effects were compared with those of vehicle-treated cisplatin administered to control and normal mice. In the serum and kidney, renal function parameters reactive oxygen species and expression of protein related to oxidative stress, DNA damage, inflammation and apoptosis were examined. Safflower seed treatment attenuated serum BUN, createinine and renal oxidative stress through reduction of reactive oxygen species and increase in the protein expression level of catalase. Safflower seed reduced renal protein expression of p-p38 and p-JNK (mitogen-activated protein kinase pathway), pro-apoptotic factors (such as Bax and caspase 3) and nuclear factor-kappa B-targeting pro-inflammatory cyclooxygenase-2 and inducible nitric oxide synthase. In addition, Safflower seed treatment led to significantly attenuated histological damage in the kidney. Conclusion : These renoprotective effects of Safflower seed were achieved through attenuation of oxidative stress and its sensitive protein expression associated with inflammation and apoptosis in cisplatin-induced nephrotoxicity mice.

To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.