줄수염나방아과는 나비목 중에서 가장 큰 분류군 중 하나인 밤나방과에 속한다. 분류군 내에서 날개무늬가 비슷한 경우가 있어 동정에 어려움이 많은 그룹 중 하나이다. 또한, 이 들은 산림 지역, 초지 및 물가 등 다양한 환경에 서식하며, 생활 습성에 따라 일부는 임업 및 농업에 피해가 큰 해충으로 알려져 있는 중요한 경제곤충 그룹이라 할 수 있다. 본 연구는 이와 같이 분류동정이 어려운 줄수염나방아과 곤충을 대상으로 야외채집조사, 표본제작, 생식기 해부검경 및 DNA 바코드 등을 수행하여 최종적으로 분류학적 동정지침서를 작성하고자 수행 되었다. 연구결과 19속 63종으로 정리되었다. 본 연구를 통해 확보된 DNA바코드 데이터는 정확한 바코드를 활용한 진단 및 동정 연구 등에 활용되고, 이들의 분포정보 구축, 형태적 특징 및 분자분석 연구의 기초데이터 확보를 통한 관련 분야 활성화 등에도 기여할 수 있을 것으로 기대된다.

The family Crambidae, so called as Grass moths, have been known as a difficult group in identification due to their similar wing patterns among the allied species. Some species are well known as pests to the agricultural and ornamental plants of economic importance. Therefore, this study was carried out to provide the DNA barcode and related information for distinguishing the complex species among Crambidae. In this study, we extracted and analyzed the DNA barcodes from 185 specimens of 76 species in the family Crambidae. Based on the result, one species of the genus Schoenobius, Pagyda, Neoanalthes, Palpita, Ecpyrrhorrhoe and Paratalanta area reported for the first time from Korea.

As a result of our taxonomic study of the genus Epistrophe (Walker) in Korea, we recognized a total of 16 species including seven putative new species (code names are temporarily given from A to F). We also conducted a DNA barcoding analysis of 25 nominal species and 130 samples of Epistrophe. In our barcoding analysis, despite insufficient bootstrap support, Epistrophe formed a cluster with two genera, Epistrophella and Leucozona, topologically supporting their close relationships. Our analysis also showed some interesting results worthy of attention: First, at least two distinct cryptic species were found within the E. grossulariae cluster. Second, the taxon identified as E. nitidicollis seems to represent at least three distinct species. Third, three BOLD-deposited European samples listed as E. olgae showed almost identical barcode sequences with the European E. nitidicollis.

In recent years, macroalgal bloom occurs frequently in coastal oceans worldwide. It might be attributed to accelerating climate change. “Green tide” events caused by proliferation of green macroalgae (Ulva spp.) not only damage the local economy, but also harm coastal environments. These nuisance events have become common across several coastal regions of continents. In Korea, green tide incidences are readily seen throughout the year along the coastlines of Jeju Island, particularly the northeastern coast, since the 2000s. Ulva species are notorious to be difficult for morphology-based species identification due to their high degrees of phenotypic plasticity. In this study, to investigate temporal variation in Ulva community structure on Jeju Island between 2015 and 2020, chloroplast barcode tuf A gene was sequenced and phylogenetically analyzed for 152 specimens from 24 sites. We found that Ulva ohnoi and Ulva pertusa known to be originated from subtropical regions were the most predominant all year round, suggesting that these two species contributed the most to local green tides in this region. While U. pertusa was relatively stable in frequency during 2015 to 2020, U. ohnoi increased 16% in frequency in 2020 (36.84%), which might be associated with rising sea surface temperature from which U. ohnoi could benefit. Two species (Ulva flexuosa, Ulva procera) of origins of Europe should be continuously monitored. The findings of this study provide valuable information and molecular genetic data of genus Ulva occurring in southern coasts of Korea, which will help mitigate negative influences of green tide events on Korea coast.