Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.

This study investigated the architectural expression of Taekpungdang(澤風堂, The Pond and Wind House) built by the Neo-Confucianist Taekdang Lee Sik(澤堂李植, 1584〜1647) from the perspective of the symbolic system of the Zhōuyì(『周易』, Classic of Changes). This study examined the historical context, personal history, and construction process of Taekpungdang at the time of its creation through his collection of writings, the Taekdanggip(澤堂集). The study also estimated the original form of Taekpungdang through field surveys and historical evidence. In addition, the architectural principles and architectural expressions inherent in the Taekpungdang were derived based on the symbolic system of “taekpungdaegwa”(澤風大過) which is Lee Sik's divination and one of the 64 trigrams in the Zhōuyì. Lee Sik, who was knowledgeable in the Zhōuyì, used divination to cope with the chaotic political situation and his own misfortunes. Accordingly, He determined the direction of his life and planned the surrounding environment, architectural structure, and form of Taekpungdang based on the rules and meanings of his divination system. He embodied the architectural space of Taekpungdang with the concept of time and space inherent in the divination of “daegwa”,(大過, great exceeding). In addition, he expressed the principle of the generation of palgue,(八卦, the eight trigrams for divination) and the principle of the co-prosperity of ohaeng(五行, the five elements) through the composition of walls and windows of the house. The images of Taekpungdaegwae, which are dongyo(棟撓wood submerged in the pond) and taekmyeolmok(澤滅木, shaking pillars), were manifested in the form of buildings. Therefore, Taekpungdang can be considered a remarkable example of a building designed through the thorough utilization of the Zhōuyì divination system.

The pataphysics implemented by digital technology differs from the form of objects in the real world and is used throughout the cultural industry. This study aims to analyze the expression method of pataphysics as applied to modern fashion and derive its impact on the fashion industry. The research analyzes fashion images, shows, films, displays, and e-commerce, since 2016, when pataphysics began to be used in the fashion domain. Pataphysics, created by Alfred Zaire, appeared as an overlapping phenomenon that reflects physical phenomena in the virtual world. The expression method of pataphysics applied to modern fashion was divided into an augmented reality method based on immersion and interaction, a virtual platform-oriented metaverse, and a virtual model expressing a processed self. The influence of pataphysics applied to modern fashion is as follows. In the field of design, pataphysics affects the development of contemplative designs for innovation and creativity. Second, digital technology can expand the role of fashion at the intersection of art and fashion that takes a novel perspective through pataphysics. Third, e-commerce positively affects efficient production and consumption through virtual and economic models. In conclusion, this study’s findings are expected to play a positive role in promoting creativity and innovation by introducing new perspectives and ideas into modern fashion through pataphysics.

Background: Serotonin receptors can be divided into seven different families with various subtypes. The serotonin 1A (5-HT1A) receptor is one of the most abundant subtypes in animal brains. The expression of 5-HT1A receptors in the brain has been reported in various animals but has not been studied in horses. The 5-HT1A receptor functions related to emotions and behaviors, thus it is important to understand the functional effects and distribution of 5-HT1A receptors in horses to better understand horse behavior and its associated mechanism. Methods: Brain samples from seven different regions, which were the frontal, central, and posterior cerebral cortices, cerebellar cortex and medulla, thalamus, and hypothalamus, were collected from six horses. Western blot analysis was performed to validate the cross-reactivity of rabbit anti-5-HT1A receptor antibody in horse samples. Immunofluorescence was performed to evaluate the localization of 5-HT1A receptors in the brains. Results: The protein bands of 5-HT1A receptor appeared at approximately 50 kDa in the frontal, central, and posterior cerebral cortices, cerebellar cortex, thalamus, and hypothalamus. In contrast, no band was observed in the cerebellar medulla. Immunofluorescence analysis showed that the cytoplasm of neurons in the cerebral cortices, thalamus, and hypothalamus were immunostained for 5-HT1A receptors. In the cerebellar cortex, 5-HT1A was localized in the cytoplasm of Purkinje cells. Conclusions: In conclusion, the study suggests that 5-HT and 5-HT1A receptor systems may play important roles in the central nervous system of horses, based on the widespread distribution of the receptors in the horse brain.

Ischemic stroke causes brain damage and neuronal cell death by depriving oxygen and nutrients and releasing excessive levels of glutamate and intracellular calcium. Epigallocatechin gallate (EGCG) is a polyphenolic compound present in green tea. It has antioxidant, anti-inflammatory, and neuroprotective effects. Hippocalcin is a calcium binding protein that regulates calcium concentration, neuronal differentiation, neuronal excitability, and neuronal cell death. In this study, we investigated whether EGCG regulates the expression of hippocalcin in neurons and astrocytes after focal cerebral ischemia. Cerebral ischemia was induced by meddle cerebral artery occlusion (MCAO). EGCG (50 mg/kg) or PBS was injected into the abdominal cavity just before MCAO surgery. Neurobehavioral tests were performed to evaluate the effect of EGCG on neurological behavioral deficits 24 h after MCAO surgery. Immunofluorescence staining was performed to evaluate the positive response to hippocalcin in the cerebral cortex after MCAO surgery. We also detected the positive reactions of neuronal nuclear protein (NeuN) and glial fibrillary acidic protein (GFAP) as markers of neuron and astrocyte, respectively. MCAO caused severe neurological impairment and EGCG treatment attenuated these impairments. MCAO damage reduced the number of NeuN-positive cells and increased the number of GFAP-positive cells. This result indicates a decrease in neurons and an increase in astrocytes. However, EGCG alleviated these changes caused by MCAO damage. MCAO reduced the number of hippocalcin-positive cells in neurons and astrocytes, and EGCG treatment attenuated these reductions. Hippocalcin exerts neuroprotective effect through regulating intracellular calcium concentration. In conclusion, EGCG regulates the expression of hippocalcin in neurons and astrocytes and has neuroprotective effects in focal cerebral ischemia.

Exposure to lipopolysaccharide (LPS) causes cognitive impairment (CI). In the preliminary study, Lactobacillus gasseri NK109 suppressed LPS-induced expression of proinflammatory cytokines in macrophages. Therefore, the effect of NK109 on LPS-increased CI was investigated in mice. Intraperitoneal injection of LPS caused CI-like behaviors and neuroinflammation. However, orally administered NK109 reduced LPS-increased CI-like behaviors and hippocampal IL-1β and TNF-α expression, whereas LPS-decreased BDNF expression increased. NK109 also reduced LPS-increased colonic myeloperoxidase, IL-1β, and TNF-α expression. The efficacy of NK109 was increased by the combination of soybean embryo ethanol extract (SE). These findings suggest that NK109 with SE can improve CI by alleviating inflammation-mediated BDNF expression, thereby being beneficial for dementia therapy.

유화 질감은 예술적 표현력을 가진 회화 기법으로 창작자의 고유한 이념과 감정을 표현해내는 주요한 수단이다. 이러한 유 화 질감의 응용을 통해 현대 유화에는 보다 다양한 회화 기법 의 표현이 가능해졌고, 현대 유화의 창작공간과 구상범위가 확 대되었으며, 이는 창작자의 아이디어 혁신을 촉진했다. 유화 질감은 모두 창작자의 고도로 숙련된 표현기술이 반영 되며 동시에 다양한 심리 상태와 정신세계를 드러낼 수 있다. 또한 예술가가 상상한 이미지를 유화 질감을 통하여 창작에 응 용할 때 작품에 대한 인지적 감상 방식이 피동적 감상에서 자발 적이고 능동적인 참여로 전환되며 이러한 참여는 감정적인 특징 을 가진다. 본 연구는 먼저 이형사신의 개념 및 발전과정을 알아보고, 유화 질감의 표현방법을 실제 회화 사례와 결합하여 분석한 후 작가의 감정을 전달할 수 있는 주요한 유화 질감 표현의 세 가 지 방법인 추상적 질감, 구상적 질감, 의상적 질감을 고찰하고 자 한다. 그리고 유화 질감의 표현 방법을 통해 그림 속에 이 형사신이 어떻게 실현되었는지 논의하고자 한다.

Patients with oral squamous cell carcinoma (OSCC) generally have an elevated expression of homeobox C6 (HOXC6) gene. We found that HOXC6 was the significantly upregulated gene in hypopharyngeal squamous cell carcinoma (FaDu) cells using RNA-seq analysis. However, it remains unclear whether HOXC6 plays a role in tumor process mechanism. Our study aimed to explore the potential oncogenic role and the detailed molecular mechanism of HOXC6 in FaDu cells. In this study, Sirt1 was validated to be overexpressed in FaDu cells and associated with HOXC6 expression. Overexpression of HOXC6 promoted the cell colony formation, whereas inhibition of Sirt1 by Sirt1 inhibitor EX527 reduced cell proliferation/colony formation and migration, and induced apoptosis in HOXC6 overexpressed FaDu cells. Interestingly, mechanistic study showed that EX527 mediated Sirt1 suppression led to decreased HOXC6 expression and upregulation of Sirt1 significantly increased the expression of HOXC6. HOXC6 was shown to cooperate with Sirt1 to enhance cell survival. We propose that HOXC6 promotes cell growth/colony formation, and that the HOXC6 may be a progression of hypopharyngeal carcinoma by activating Sirt1 pathways.

어류의 번식은 뇌에서 분비되는 다양한 신경호르몬과 뇌하수체에서 분비되는 생식소 자극 호 르몬에 의해 조절된다. 극동산 뱀장어(Anguilla japonica)의 번식도 이 호르몬들의 작용에 의해 조절되지만 성 성숙 시 신경호르몬이 뇌하수체 호르몬을 조절하는 방법은 완전히 밝혀지지 않 았다. 이전 연구에 의하면 progesterone (P4), melatonin 및 serotonin (5-HT) 등과 같은 신경호 르몬이 일부 어류의 번식 과정 조절에 관여하는 것으로 밝혀졌다. 본 연구에서는 뱀장어의 뇌 하수체를 초대 배양하였고, 안정화된 뇌하수체 세포에 P4, 17β-estradiol (E2), melatonin 및 5- HT를 처리하였다. 이후 처리된 호르몬의 작용이 뇌하수체 세포에서 번식 관련 호르몬인 FSHβ, LHβ, GH 및 SL mRNA 발현에 어떤 영향을 미치는지 조사하였다. 본 연구를 수행한 결과, P4는 뇌하수체 세포에서 FSHβ와 LHβ 발현을 증가시켰고, melatonin은 FSHβ와 LHβ 뿐만 아니라 GH 와 SL의 발현을 증가시켰다. 하지만 5-HT는 이 유전자의 mRNA 발현에 유의한 영향을 미치지 않았다. 이상의 결과는 P4 또는 melatonin이 뱀장어의 초기 성 성숙에 중요한 역할을 할 수 있음을 의미한다.

이 연구는 한국어를 학습하는 외국인 학습자들을 위하여 인지언어학적 인 관점으로 접근하여 신체 관련 한국어 감정 관용 표현에 나타나는 은 유와 개념화의 방식을 살펴보는 데 목적이 있다. 관용 표현은 그 언어를 사용하는 사람들에 의해 그 의미가 형성된다. 따라서 관용 표현을 생성 한 한국인의 개념화 방식을 알아야 한다. 이에 따라 분노와 불안의 근원 영역을 ‘온도/색깔/명암/전복/분출/경직’으로 분류하였다. 근원 영역이 ‘온도’일 때 개념적 은유는 ‘분노는 뜨거움’과 ‘불안은 차가움’이며 ‘색깔’ 일 때의 개념적 은유는 ‘불안은 죽음’, ‘명암’의 근원 영역에서는 ‘불안은 어두움’이라는 개념화가 일어나고 ‘전복’의 영역에서는 ‘분노는 뒤집함’이 라는 개념적 은유가 일어난다. 또한 ‘분출’은 ‘불안은 땀’이라는 개념적 은유를, ‘경직’은 ‘불안은 정지’라는 개념적 은유를 통해 감정 관용 표현 을 생성하였다. 본고는 신체 관련 한국어의 감정 관용 표현 통해 한국인 의 개념화 방식을 정리하였으며 이 연구가 한국어교육 현장에 기초 자료 로도 활용될 수 있다는 점에서 의의가 있다