Single nucleotide polymorphisms (SNPs), that usually occurs with a modification in single nucleotide among the population of 1000 nucleotides. Such changes in nucleotides have been investigated and been associated by meat scientist for economically important traits and to increase economical profits in stock breeding. Pluralities in the study have correlated SNPs of potential candidate gene with economically important traits in domestic animals have been put forward. In chickens, INS, IGF1, IGF1R, IGFBP, CAPN1, CAPN3, GHSR, FATP1, FGFBP1, FGFBP2, apoVlDL-Ⅱ, PB1, miR-1614-3p, DAAM1, Wnt3A, LRP5, CHP, RHOA, MAPK9, SFRP1, ATGL, PGC-1α, NPY, GnRHR, PRL, TGFβ2, CASR. UCP, ADSL, STAT5b, LRP2 and CTSD genes have been found to have significant effects on body weight, breast muscle weight, carcass weight and egg number. For the similar reasons, SNPs of these genes have been considered useful DNA markers for the improvement economic value of poultry. Although further studies on different breeds of chickens would be required to segregate such dataset for different breeds of chickens.
Analytical method using capillary GC/ECD was developed to determine trace residues of chlorfluazuron, 1-[3, 5-dichloro-5-trifluoromethyl-2-pyridyloxy)phenyl)-3-(2, 6-difluorobenwyl), in meat, and applied to analyze the residues in domestic and imported meats. The analytical scheme developed does not require column chromatographic cleanup; chlorfluazuron was extracted with diethyl ether and petroleum ether (50: 50), partitioned against acetonitrile, cleaned up with silica Sep-Pak cartridge, identified GC/ECD, and comfirmed by GC/MS. The mean recoveries of the pesticide in meat fortified with standard solution 0.1, 0.5, 1.0 mg/kg were ranged from 82 to 95%. The limit of detection and limit of quantitation were 0.001 and 0.005 mg/kg, respectively. Chlorfluazuron residues were not found in domestic samples, but found in imported Australian beef ranging from 0.02 to 0.17 mg/kg, detected by 18% among the samples.