The root-lesion nematode Pratylenchus spp. is the most important plantparasitic nematode due to its worldwide distribution, wide host ranges, and migratory endoparasitic characteristics. One population of Pratylenchus collected from the giant pussy willow (Salix chaenomeloides Kimura) in the Andong area as part of a nematode survey in Korea was characterized morphologically and by molecular methods. The analysis of morphological measurements and morphometric characteristics, as well as DNA sequencing of the rRNA large subunit (LSU) D2/D3 expansion segments and the internal transcribed spacer (ITS) gene sequence, confirmed the identity of this population as P. hippeastri. This study is the first report of P. hippeastri associated with Salix chaenomeloides in Korea and worldwide. Further studies on distribution and pathogenicity in different P. hippeastri host crops, such as grapevines, strawberries, and apples, are necessary. The taxonomic keys to 16 Pratylenchus species in Korea are provided.

산화아연 막은 투명한 전도성 물질로써 다양한 분야의 광전자소자에 이용되고 있다. 그러므로 산화아연 막의 특성을 규명하는 것은 광전자소자의 성능을 높이는데 매우 중요한 역할을 할 것이다. 본 논 문에서는 이러한 산화아연 막을 용액공정 기반으로 제작하여 형태적, 구조적 특성을 평가하고자 한다. 구 체적으로는 졸-겔 방법을 반복적으로 시행하여, 시행 횟수에 따른 산화아연 막의 물성의 변화를 관찰할 것 이다. 일정한 용액 조건하에서, 5회의 반복적인 졸-겔 방법을 시행한 결과 결정화가 진행되는 것을 확인하 였다. 7회 이상에서는 원소 구성 및 결정화도가 특정 값에 수렴하는 경향을 보였다. 최종적인 산화아연 막 의 평균결정의 크기는 약 10.7 nm 정도로 계산되었다. 본 연구를 통해 최적의 결정화를 보이는 공정횟수 는 7회였다. 본 연구 결과 및 방법론은 다양한 용액공정 변수를 가변시키면서 적용할 수가 있고 최적의 공 정조건을 확립하는데 기여할 것으로 기대한다.

The sugar beet cyst nematode (SBCN), Heterodera schachtii is a well known pathogen on Chinese cabbage in thehighland fields of Korea. However, recent reports have shown a coexistence of SBCN with the clover cyst nematode,H. trifolii in Korea. This study aimed at detailing and reviewing morphological and molecular characteristics conceivablyessential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juvenilesand vulval cones of cysts showed significant differences between the two cyst nematodes. Indeed, using assorted cystsfrom field populations for molecular analysis resulted into a mixture of bands after gel electrophoresis, indicating a combinationof the two species. Thus, this study confirms the coexistence of the two species on Chinese cabbage fields in Korea.

본 연구는 둥글장수풍뎅이(Pentodon quadridens bidentulus (Famaire, 1887)) 유충의 혈림프에 존재하는 혈구세포들의 종류와 병원균 침 입시 세포 면역을 담당하는 세포를 특정하고 분석하였다. 둥글장수풍뎅이(P. quadridens bidentulus)유충 혈강내에는 과립혈구세포, 부정형혈구 세포, 편도혈구세포, 소구형혈구세포, 원시혈구세포, 지방혈구세포 총 여섯 종류의 혈구세포들이 존재하였다. 여섯 종류의 혈구세포 중 과립혈구 세포가 병원균 침입 시 형태적 변이와 함께 병원균들에 대한 식균작용을 수행하는 면역세포로 관찰 되었다. 특히, 생체 내에서 병원균 침입시 12 시간 안에 활발히 식균작용을 수행하였고 24시간 이후로는 면역 활성화가 줄어들면서 정상상태로 전환되는 것을 알 수 있었다. 세포질 혈구세포 의 식균작용도 간혹 관찰 되었으나 나머지 세포들은 면역과 연관된 활성화가 없는 것으로 나타났다.

In the present study, a severe outbreak of Sclerotium rot on Korean melon(Cucumis melo L. var. makuwa Makino) observed in Jinju, Korea in August of 2014 and 2015 is reported. The fruit rot disease on the surface of Korean melon fruit appeared as water soaked lesion followed by the development of large rotten areas with abundant white mycelial growth. Abundant white mycelial mats with globoid and white to brown sclerotia(1-3 mm) were also developed on stems near the soil line, and infected plants withered. The fungal pathogen was isolated on potato dextrose agar(PDA) and formed white colonies with numerous sclerotia on PDA. The sclerotia were globoid in shape, 1-3 mm in size, and white to brown in color. The mycelium formed typical clamp connection. These measurements and taxonomic characteristics were most similar to those of Sclerotium. DNA sequencing and phylogenetic analysis of the complete internal transcribed spacer rRNA gene region confirmed that the fungal isolates were Sclerotium rolfsii Saccardo. Koch’s postulates were supported by pathogenicity tests conducted on fruit. Based on mycological characteristics, pathogenicity tests, and molecular identification, the fungus was identified as Sclerotium rolfsii. To the best of our knowledge, this is the first report of a Sclerotium rot caused by S. rolfsii on Korean melon in Korea.

In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines

The studies on activated carbon prepared from walnut shell and groundnut shell were undertaken to ascertain the effect of initial state of precursor and activation process on the development of porosity in the resulting activated carbon. Walnut shell based carbon shows the presence of cellular pores while Groundnut shell based carbon shows fibrillar pore structure. The adsorption parameters, characterization of product and scanning electron microscopic studies carried out showed the presence of mainly Micro, Meso and Macro porosity in carbon prepared from Walnut shell while mainly micro porosity was observed in Groundnut shell based activated carbon. An interrelationship between the adsorption efficiency and porosity in terms of quality control parameters, for before and after activation, was validated through the scanning electron microscopic data.

PDA배지에서 25℃로 배양할 때 초기 균사의 색은 흰색을 띄고 있었으며 배양 후기에 생성되는 포자의 색은 짙은 푸른색을 나타내고 있었다. 시간당 성장률은 1.1mm보다 빠른 것으로 나타났으며 성숙한 포자의 모양은 부드럽고 구형에 가까운 모양이었다. 이러한 형태학적 결과는 시간당 성장률이 0.5-0.7mm 이하인 Tricho-derma viride 및 T. hamatum의 특징과 성숙 포자의 모양이 실린더 모양인 T. koningii,

Off-type rice plants occurring in farm fields cause yield loss due to competition with cultivated rice, in addition to hindering field management and harvest work. This study aimed to observe the agronomic characteristics and trace the origins of off-type rice plants using molecular markers. A total of 116 rice accessions, comprising 35 off-type plants collected from Korean farm fields, 19 Korean commercial cultivars, 12 Korean land races, and 50 weedy rice collections, were phenotyped and genotyped using selected SSR and Subspecies Specific (SS)-STS markers. The results showed that the plant height, culm length, and leaf length of off-type rice plants were larger than those of cultivated rice, which is the typical phenotype of weedy rice. However, off-type plants were highly sterile, as opposed to weedy rice, which were highly fertile. Genotype analysis with SSR and SS-STS markers revealed that off-type rice plants were heterozygous at most of the tested marker loci, suggesting that the off-type rice plants may have originated from natural outcrossing. The genotypes of off-type rice plants were closely related to both weedy and cultivated rice, and the phylogenetic analysis revealed that the relationship of the clustered group of offtype rice plants is intermediate between Indica type weedy rice and Japonica type commercial varieties. These results suggested that off-type rice plants collected in Korean farm fields might have originated from natural outcrossing between Indica type weedy rice and the cultivated Japonica type commercial varieties.

This study was done to characterize the anther derived regenerants (R1) including haploids and spontaneous diploids of minipaprika (cvs.‘Vine sweet-red’ ‘Vine sweet-yellow’ and ‘Vine sweet-orange’) in glasshouse. Eleven haploids (three, seven and one from red, yellow and orange, respectively) and sixteen spontaneous diploids (five, nine and two from red, yellow and orange, respectively) were grown in plastic pot with three (red, yellow and orange) anther donor (R0) F1minipaprika varieties. Regenerants were characterized for their plant and fruit characters as well as their fruit color and shape. The homozygosity of spontaneous diploid plants of each population was assessed using simple-sequence repeat (SSR) marker analysis. Haploid plants were characterized by reduced plant height, small leaves, short petiole and internode and small flower bud and all haploids showed the sterility and vice-versa in spontaneous diploid lines. The fruit biometrical traits exhibited greater variation within the spontaneous diploid plants and average value of quantitative traits is lower than standard varieties. MR-4 gave the highest yield (150.5 g) per plant followed by MY-6 (140.0 g) and MY-8 (130.5 g) and the lowest in MY-5 (31.5 g). Morphological marker such as fruit color further determinedthe microspore origin of androgenic diploids obtained in anther culture of ‘Vine sweet-red’. Of the fifteen spontaneous diploid plants, fourteen plants were identified as doubled haploids using microsatellite markers (SSR), and these homozygous lines are recommended to use in minipaprika breeding program.

Anthers of sweet pepper F1 cultivar ‘Special’ were cultured on Dumas De Vaulx (C medium), supplemented with 0.1mgL-1 2, 4-D and 0.1 mg · L-1 kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and 9.8 ºBrix, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing γ-TMT gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding γ-Tocopherol methyltransferase gene (γ-TMT) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the T0 and T1 generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. T1 progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of T1 progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.