말채나무공깍지벌레의 계절적 발육을 2019년 6월 4일(약충) 부터 2020년 6월 25일까지(1세대 약충) 경상남도 사천시 소재의 블루베리 과 원에서 관찰하였다. 본 충의 발육을 연구하기 위해서 2018년 눈에서 발아한 5개 이상의 잔가지를 1주 간격으로 농가에서 채취하였다. 깍지벌레의 발육은 실체현미경 하에서 조사하였고 화학적 방제는 시중에서 이용할 수 있는 3종의 살충제로 블루베리 과원에서 수행하였다. 발육기간과 유효적산온도(Centigrade Degree-Days accumulation, DDC)에 대한 결과는 다음과 같다. 산란기간(최성기): 2020년 5월 12 -26일(DDC, 110.0-188.5(173.6)); 부화기간(최성기): 2020년 6월 9 - 23(19)일)(DDC, 325.2-480.8(435.6); 난기간: 26 일; 월동성충으로부터 약충의 신엽으로 이동: 2020년 6월 16-25 일(DDC, 410,5 - 500.4); 성충으로 발육하기 위한 잎에서 잔가지로 이동(최성기): 2020년 2월 4 -18(8)일. 성충 한 마리 당 산란수(범위): 956.8 ± 73.4 (13 - 3497); 알의 크기(mm): 0.29 ± 0.020(L), 0.15 ± 0.013(W); 부화약충의 크기: 0.35 ± 0.018(L), 0.18 ± 0.007(W), 0.09 ± 0.007(눈 간격); 성충크기: 4.30 ± 0.893(L), 2.64 ± 0.520(W). 월동성충으로부터 부화한 약충은 신초의 잎 뒷면에서 다음해 2월 초순 잎이 떨어질 때까지 약 95%가 발견되었다. 이들은 2령충으로 월동하고 1년에 한 세대를 경과하였다. 기어다니는 1령충을 방제 하기 위하여 3종의 살충제를 7월 16일과 30일에 거북밀깍지벌레에 등록된 농도로 처리하였다. 아세타미프리드수화제가 1회 처리 21일 후에 96.9%의 사충율을 나타내었다.

느티만가닥버섯 ‘햇살’ 품종에 대한 봉지재배 안정생산 재배기술을 개발하기 위하여 포플러(미루나무) 톱밥을 주배지로 하고 밀기울, 미강, 건비지, 면실박, 팜커널등 5종 의 영양원배지 재료를 혼합하여 배지를 조성하여 재배시험을 수행하였다. 배지혼합 전 재료들에 대한 열수추출 배지들에 대한 버섯균사 생장은 팜커널에서 가장 우수하였으며 균사밀도의 경우 포플러배지를 제외하고 모두 우수하였다. 조성배지들에 대한 버섯발생 안정성, 재배기간, 수량성을 기준으로 조사한 결과 포플러톱밥, 밀기울, 미강, 건비지, 팜커널이 55:15:20:5:65(v/v) 수준으로 조합된 T6 배지에서 생육기간이 단축되고 수량이 높아 봉지재배에 적합한 배지로 선발하였다. 선발배지에 대한 균사배양 완료기간은 38.0일로 대조로 이용된 T1배지에 비하여 1.7일 더 소요되었다. 그러나 균긁기 이후 수확시까지 소요되는 기간은 18.0일로 대조배지(T1)를 이용한 재배기간보다 1.0일 단축됨에 따라 총 재배기간은 68.0일이 소요되었다. 선발배지에 대한 수량은 144.7 g으로 대조배지(T1) 수량 112.5 g보다 약 28.6%가 증가되었다. 이상의 연구결과를 통해 느티만가닥버섯 ‘햇살’ 품종의 봉지재배 적합배지로 포플러톱밥, 밀기울, 미강, 건비지, 팜커널이 55:15:20:5:5 (v/v) 비율로 혼합된 배지조성을 선발하였다. 선발배지에 대한 이화학적 특성으로 수분함량은 60.8%, pH 6.0, 전질소 함량은 0.83% 수준이었다.

In March of 2015, severe sooty mold was observed on blueberries cultivated in a farmer’s greenhouses in Goseong-gun, Gyeongsangnam-do, South Korea. Typical symptoms included a black or dark brown, superficial fungal growth on the leaves, stems, and fruit of blueberry plants. The fungal colonies on PDA were flat and black. The conidia were dark green in color, globose to sub-globose in shape and 3-5×3-4 μm in size. The ramoconidia were cylindrical and 18-42×3 μm in size. The conidiophores were dark brown, cylindrical-oblong in shape and 42-138×3-4 μm in size. To confirm the identity of the fungus(F130), the 18S rDNA as well as actin and β-tubulin gene sequences were PCR-amplified and sequenced. The pathogenicity of the isolate was then explored on 2-month-old potted blueberry plants. Based on the mycological characteristics, molecular identification, and pathogenicity on host plants, this fungus was identified as Cladosporium sphaerospermum. This is the first report on sooty mold of blueberry caused by C. sphaerospermum in Korea.

Circular leaf spot(CLS) disease causes considerable economic damage to persimmon(Diospyros kaki) in South Korea. Mycosphaerella nawae ascospores are the primary inoculum for CLS epidemics. In this study, we investigated the seasonal fluctuation of spore release and its relationship to environmental factors, based on spore trapping. We evaluated the seasonal pattern of released M. nawae ascospores in South Korea. During three persimmon growing seasons(2010 to 2012), we recorded the release of ascospores in two regions, Jinju and Gimhae, which are major producing regions of sweet persimmon in South Korea. The release of CLS ascospores was from the first week of May to the end of July. The maximum release of spores was observed in late June. A computer model used accumulated degree days to simulate ascospore release. The overall mean accumulated degree days, from 01 May to 50% ascospore release for the observed data(1174℃ days), was not significantly different from the predicted value of 1144℃ days. The mean differences between predicted and observed release percentages for the sampling periods were not significantly different from zero.

단감원에서 식나무깍지벌레의 발생을 최소화하고자 시기별 발생특성과 우수 방제약제를 선발하였다. 가지에서 월동한 약충이나 암컷 깍지 는 9월 중순경에 교미 후, 월동에 들어가며 다음 해 봄철에 기온상승과 더불어 포란과 산란의 과정을 거쳐, 5월 중하순까지 산란을 마친 후 죽었 다. 수컷은 대부분 9월 말까지 우화하여 암컷과 교미하고 죽었으나 낙엽에서 월동하는 개체의 경우 모두 사멸하였다. 식나무깍지벌레의 산란 특 성은 매년 기상상황에 따라 차이가 있었고, 4월 중순부터 5월 중·하순까지 산란하고, 5월 초․ 중순에 산란 최성기를 보였다. 산란 량은 160개 정 도로 추정되며, 산란 최성기로부터 약 1주일 경과 후 부화 최성기를 보였다. 여름철에는 7월 초․ 중순에 시작하여 8월 중․ 하순까지 산란하고, 7월 말과 8월 초에 산란 최성기에 도달하며, 암컷 한 마리당 산란수는 봄철보다도 다소 적은 130개 정도이고, 난 기간은 약 4일로 추정되었다. 가는 가지에서 월동한 성충이 산란한 알로부터 부화한 1세대 약충은 가지와 잎에서 각각 10%, 90% 정도의 비율로 관찰되었다. 가는 가지에서 대부분 암컷 깍지로 발육하고 2009년 7월 27일 2세대 약충 발생 최성기에 도달한 다음 8월 중 ․ 하순경부터 월동처로 정하고 살아간다. 암컷과 수컷 깍지 는 8월 이전까지 잎에서 거의 비슷한 비율로 발육하다가, 2009년 8월 12일 이후 잎에서 수컷의 약충 발생 최성기를 시작으로 8월 27일 수컷 깍지 벌레 발생 최성기, 9월 14일 전후로 수컷 성충의 발생에 이르기까지 일관된 발생관계를 관계를 보여주었다. 8월 12일 잎에서 관찰되는 약충 중 75% 정도는 발육하여 수컷으로 우화하여 교미하고 죽는 것으로 나타났다. Buprofezine+dinotefurn (20+15) WP로 6월 9일과 16일 2회 방제 하고 7주 후에 생사충을 판정한 결과 방제가가 90.6%로 나타났다.

In the present study, a severe outbreak of Sclerotium rot on Korean melon(Cucumis melo L. var. makuwa Makino) observed in Jinju, Korea in August of 2014 and 2015 is reported. The fruit rot disease on the surface of Korean melon fruit appeared as water soaked lesion followed by the development of large rotten areas with abundant white mycelial growth. Abundant white mycelial mats with globoid and white to brown sclerotia(1-3 mm) were also developed on stems near the soil line, and infected plants withered. The fungal pathogen was isolated on potato dextrose agar(PDA) and formed white colonies with numerous sclerotia on PDA. The sclerotia were globoid in shape, 1-3 mm in size, and white to brown in color. The mycelium formed typical clamp connection. These measurements and taxonomic characteristics were most similar to those of Sclerotium. DNA sequencing and phylogenetic analysis of the complete internal transcribed spacer rRNA gene region confirmed that the fungal isolates were Sclerotium rolfsii Saccardo. Koch’s postulates were supported by pathogenicity tests conducted on fruit. Based on mycological characteristics, pathogenicity tests, and molecular identification, the fungus was identified as Sclerotium rolfsii. To the best of our knowledge, this is the first report of a Sclerotium rot caused by S. rolfsii on Korean melon in Korea.

A severe outbreak of gray mold on kenaf (Hibiscus cannabinus L.) was observed on kenaf grown in the research field of Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, Korea in 2014. Gray mold appeared on young plants as gray-brown velvety mold covering stems and leaves. Infections that girdled the stem caused wilting above the infected area and developed a canker. The casual fungus formed grayish brown colonies on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, colorless or pale brown in color, and 6–18 × 4–10 μm in size. The conidiophores were 15–32 μm in length. These measurements and taxonomic characteristics were most similar to those of Botrytis. DNA sequencing and phylogenetic analysis of the complete internal transcribed spacer rRNA gene region confirmed that the fungal isolates were indeed Borytis cinerea. Koch's postulates were supported by pathogenicity tests conducted on healthy plants. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea. To the best of our knowledge, this is the first report of a gray mold caused by B. cinerea on kenaf in Korea.

From August to October 2013, a powdery mildew was found on Japanese artichoke(Stachys sieboldii) in Jinju, Korea. White colonies were present on leaves and petioles. Severely infected lesions were discolored, being brown. In the present study, the morphological features of anamorphic and teleomorphic Korean specimens were studied. To complete the identification, the sequence of the internal transcribed spacer region of the ribosomal RNA gene(ITS rRNA) was analyzed. Based on morphological characteristics, including anamorphic and teleomorphic features, as well as analysis of the ITS rRNA gene region, the fungus associated with the symptoms was identified as Neoerysiphe galeopsidis. Although the host ranges of powdery mildew caused by N. galeopsidis has been previously recorded, no full description or illustration of fungal symptoms and signs on Japanese artichoke has yet appeared. To the best of our knowledge, this is the first full description of powdery mildew disease on Japanese artichoke featuring molecular identification, morphological features, symptoms, and signs in Korea.

From August to October of both 2012 and 2013, powdery mildew was observed on white-fruited creeping cucumber(Melothria japonica [Thunb.] Maxim.) at Jinju, Korea. White mycelial colonies were observed on leaves and petioles. Lesions of severe infections were discolored or brown. In the present study, the morphological features of the anamorphic and teleomorphic reproductive stages of the fungus were studied. To complete the fungal identification, the sequence of the internal transcribed spacer region of a ribosomal RNA(ITS rRNA) gene was determined. Based on morphological characteristics and analysis of the ITS rRNA gene, the fungus causing the powdery mildew symptoms was identified as Podosphaera fusca. Although the host range of powdery mildew caused by P. fusca has been previously described, no full description and illustration of the infection, including symptoms and signs on white-fruited creeping cucumber, has yet appeared. To the best of our knowledge, this is the first full description of powdery mildew on white-fruited creeping cucumber, featuring molecular identification, symptoms, and signs.

Rhizopus fruit rot was observed on strawberry fruit (Fragaria x ananassa Duchesne) grown in a glass greenhouse at the Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, South Korea, from 2011 to 2013. Symptoms included water-soaking, rapid softening, and rotting. When these symptoms were left untreated, vigorous fungal growth was observed on the surface of infected fruits. Colonies on potato dextrose agar (PDA) at 30°C were white-cottony to brownish-black. Sporangia were globose, black, and 40–210 ㎛ in diameter. Sporangiophores were light-brown and 6–22 ㎛ in diameter. Sporangiospores were globose to oval, brownish, streaked, and 4–12 ㎛ in length. Columellae were light brownish gray, hemispherical, and 80– 120 ㎛ in diameter. To confirm the identity of the causal fungal pathogen, the complete internal transcribed spacer (ITS) ribosomal RNA gene region was amplified and sequenced. Based on these symptoms, mycological characteristics, pathogenicity tests on host plants, and molecular identification, the fungus was identified as Rhizopus oryzae Went & Prinsen Geerligs. This is the first report of Rhizopus fruit rot on strawberry caused by R. oryzae in Korea.

A rust on Asiatic dayflower plant (Commelina communis L.) was found in Geoje, Korea, in August 2010. Uredia are mostly produced on abaxial leaf surface or elongated on stem, early naked, surrounded by the ruptured epidermis, cinnamon-brown. Uredospores are globose, ellipsoid or ovate, echinulate, yellowish brown or brown, 20-30 × 20-25 ㎛. On the basis of mycological characteristics and molecular data, the fungus was identified as Uromyces commelinae Cooke. The phylogenetic position of U. commelinae is separate from the other rusts where the economically important rusts of the Poaceae are situated. Although host ranges of the rust caused by U. commelinae were previously recorded, full descriptions and illustrations, including symptoms and signs have not been described. This is the first description of rust disease on C. communis plant with molecular identification, symptoms, and signs.

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.