식품에서 대장균군을 효율적으로 분리하기 위해 대장균군 선택배지 5종: Chromocult Chromocult coliform agar (Merck), Hicrome coliform agar (Sigma), CHROMagar ECC, Brilliance E.coli/coliform medium (OXOID), endo agar (Merck)을 선별하여 식품 분리 대장균군 83종 및 표준균주 21종에 대해서 민감도와 특이도를 분석한 결과 Chromocult coliform agar와 HICrome coliform agar에서 94%도 민감도가 가장 높았으며, Brilliance E.coli/coliform medium는 93%, CHROMagar ECC는 92%, ENDO agar는 74%의 민감도를 나타냈다. Chromocult coliform agar와 HICrome coliform agar는 대장균군의 회수율도 높았다. 그러므로 Chromocult coliform agar와 HICrome coliform agar는 대장균군을 분리하는데 가장 효율적인 배지로 생각된다.

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird- Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat (60oC for 90s), and cold (−20oC for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

In agricultural fields, the entomopathogenic fungal species have been investigated for their potential as the biological control agents due to their role of natural enemies for insects. Until recent times, most of the studies for these fungi have been based on isolation from insect cadaver or soil. However, these methods, especially isolation from soil, might cause a problem involving differential isolation of the each entomopathogenic fungi. The purpose of this study is to determine the optimal isolation medium for entomopathogenic fungi using dodine, chitin, and skim milk. The growth rates of entomopathogenic fungi and non-entomopathogenic fungi were compared on dodine agar medium. The medium for this experiment was modified Veen semiselective medium which consisted of based on SDA (Sabouraund Dextrose Agar), 100 mg/ml for chloramphenicol, 50 mg/ml for streptomycin and adjusted dodine to 40, 50, 70 and 100 mg/ml. As a result, optimal concentration of dodine for isolation of entomopathogenic fungi was 50 mg/ml and 168 positive entomopathogenic fungi were isolated in 470 soil samples and 11 cadavers of insect. In addition, the isolates had significantly greater chitinase and protease activity than non-entomopathogenic fungi. The isolation method described represents a valuable tool for rapid and simple isolation of entomopathogenic fungi. These positive entomopathogenic fungi may have potential against variety pests in agriculture.