This study investigated various levels of sodium nitrite and probiotics (SNPro) combination as an alternative to zinc oxide on the growth performance, immune response, intestinal microflora, and morphology of weaned pigs. One hundred and ninety-two weaned pigs (Landrace×Yorkshire×Duroc) with an average body weight of 6.51±0.15 kg were randomly assigned to four treatments(n=6) on the basis of their initial body weight. Experimental period was divided into phase 1 and 2 (each 14 days). The dietary treatments were: 1) Basal diet (control), 2) SNPro1 (control+0.01% SNPro), 3) SNPro2 (control+0.02% SNPro), 4) SNPro3 (control+0.03% SNPro). The average daily gain when SNPro was added to the diet was 288, 309, 319, 324 g in phase 1, 355, 387, 410, 407 g in phase 2 and 321, 348, 364, 366 g in the overall. The concentration of interleukin-8 and interleukin-10 in serum when SNPro was added to the diet were 15, 13.5, 13, 12.8 ng/ml and 165, 162, 155, 145 ng/ml (p<0.05) but toll-like receptor 4 and immunoglobulin G levels in serum were no significantly different. The colonization of Escherichia coli in the ileum and Salmonella spp. in the caecum were significantly decreased as SNPro level increased (p<0.05). However, the population of Lactobacillus spp. did not differ among the groups. Although villus height and villus height to crypt depth ratio were not significantly affected by the treatments, crypt depth in the jejunum was 599, 586, 615, 599 ㎛ as SNPro level increased (p<0.05). In conclusion, SNPro had beneficial effects on growth performance, immune response, intestinal microflora and morphology weaned pigs. Therefore, SNPro not only can be considered as an alternative for the pharmacological level of zinc oxide in weaning pigs but also ideal dietary SNPro level was 0.02%.

This study investigated the antioxidant properties of the Radix isatidis extract and the effect of substituting it with sodium nitrite (SN) in a pork patty. The antioxidant activity of the Radix isatidis water extract (RIWE) was compared to those of the methanol and chloroform extracts (RIME and RICE, respectively). Patties were prepared with five different treatments: (1) a control with no RIWE and SN, (2) T1 with 150 ppm SN, (3) T2 with 100 ppm SN + 0.1% RIWE, (4) T3 with 50 ppm SN + 0.3% RIWE, and (5) T4 with 0.5% RIWE. They were used to compare the oxidation stability for 12 days at 4℃. The extraction yield of RIWE was 3.2 and 18.7 times higher (p<0.05) than those of RIME and RICE, respectively, but RIWE had the lowest (p<0.05) 1,1`-diphenyl-2-picrylhydrazyl radical scavenging activity, superoxide dismutase-like activity, and total reducing ability. Patties with RIWE and SN exhibited lower (p<0.05) pH values after 12 days, compared to the control. After 4 days, the 2-thiobarbituric acid reactive substances content was lower (p<0.05) in T1, T2, T3, and T4 than in the control. The oxidation-reduction potential value was the lowest (p<0.05) in T3 and T4 after 4 days (p<0.05). T1, T2, T3, and T4 had a lower (p<0.05) volatile basic nitrogen content after 12 days, compared to the control. These findings suggest that RIWE has an antioxidant effect similar to that of SN, but further research on its antimicrobial effect may be required to determine if it is a good substitute for SN.

To investigate effects of starvation on physiological changes, stress response, and survival of cobitid loach (Misgurnus anguillicaudatus) exposed to sodium nitrite (NaNO2), a 4- week experiment was conducted. Fewer fish survived in the starved group than those in the fed group during the experiment. Starvation resulted in growth retardation, leading to differences in body length and body depth between fed and starved groups. The fed gorup continued to grow and remained in good condition. Blood chemical analysis (plasma cortisol and glucose) showed significant differences in stress response to nitrite exposure between fed and starved groups (p < 0.05). These results suggest that all parameters employed in this study to assess effects of starvation with NaNO2 stress are useful information for researching nutritional status in cobitid loach.

본 연구는 아질산나트륨 및 비타민 C의 대체로 첨가한 수벌번데기 분말이 유화형 소시지의 이화학적 품질 특성에 미치는 영향을 구명하고자 하였다. 시료는 아질산염, 비타민 C 및 수벌번데기 분말의 첨가 수준에 따라 각각 아질산나트륨 150 ppm+비타민 C 200 ppm(대조구), 아질산나트륨 75 ppm+ 비타민C 100 ppm+수벌번데기 분말 6.015%(T1) 또는 수벌번데기 분말 12.03%(T2)을 첨가하여 제조한 후 4℃에 30일 동안 저장하였다. pH는 수벌번데기 분말의 첨가 수준이 증가함에 따라 현저하게 감소하였다(p<0.05). 일반성분 함량은 수벌번데기 분말의 첨가 수준이 높은 소시지에서 낮은 수분 및 조단백질 함량과 높은 조지방 및 조회분 함량을 보였다 (p<0.05). 지방산 조성은 T1과 T2가 대조구에 비해 포화지방산 함량이 높았으나(p<0.05), 불포화지방산 및 다가불포화지 방산 함량은 낮았다(p<0.05). 색깔은 T1과 T2가 대조구보다 현저하게 낮은 명도(L*) 및 적색도(a*)와 높은 황색도(b*) 및 hue-angle(h°)을 보였으며(p<0.05), T2의 경우 가장 낮은 chroma(C*)를 나타내었다(p<0.05). 지방산화물(TBARS) 함량은 T2가 가장 높았으며(p<0.05), 특히, 대조구보다 2배 이상 높았다. 조직감은 T1과 T2가 저장기간 동안 대조구에 비해 단단한 특성을 보였다(p<0.05). 따라서 유화형 소시지에서 수벌번데기 분말의 첨가는 색깔, 지방산화안정성 및 조직감에 부정적인 영향을 미치는 것으로 나타나, 아질산나트륨 및 비타민 C 대체 효과가 없었다

This study was performed to evaluate physicochemical properties of model sausages with various levels of pressed cherry fruit extracts alone or in combination with tomato powders as a partial replacement of sodium nitrite. In study 1, physicochemical properties of model sausages were not affected by the addition of pressed cherry fruit extracts(p>0.05), except for Hunter L(lightness) and Hunter a(redness). Model sausages containing pressed cherry fruit extract(T1, 0.5% and T2, 1.0%) decreased lightness, whereas redness of REF(156 ppm sodium nitrite) was not different from T2(p>0.05). However, those with cherry fruit extract at 1% were proper to improve the hunter color values, especially redness values. In study 2, physicochemical properties of model sausages were not affected by the addition of pressed cherry fruit extract and tomato powders in selected nitrite contents, except for color and cooking loss(p>0.05). In color values, REF was the highest level, and T2, T4, and T5 was significantly low level in lightness (Hunter L). T2 was the highest value in cooking loss(%) among other treatments. The redness values(Hunter a) of T5(75ppm, nitrite, 1% pressed cherry fruit extract and 0.5% tomato powder) were not different from REF which had highest redness values(P<0.05). In conclusion, the combination of pressed cherry fruit and tomato powder might be considered as an alternative method to partially replace with sodium nitrite in pork model sausages.