목적: 본 연구는 대학생에서 콘택트렌즈 착용 여부에 따라 음주와 전자기기 사용이 안구건조 증상에 미치는 영향을 조사하였다. 방법 : 대학생 32명(64안)을 콘택트렌즈 착용군(16명)과 미착용군(16명)으로 나누었다. 순목 횟수, 비침입성 눈 물막 파괴검사(NIBUT), 오큐튜브 검사를 기본 검사, 스마트폰 20분 시청 후, 목표 혈중 알코올 농도 0.03% 달성 후 측정하였다. 결과: NIBUT는 미착용군에서 착용군보다 일관되게 낮았으며, 기본 검사 및 전자기기 사용 후 유의한 차이를 보였다 (p=0.010, p=0.001). 음주 후에는 경계선상의 유의성을 보였다(p=0.057). 오큐튜브 검사값은 미착용군에서 높았으며, 음주 후 유의한 차이를 나타냈다(p=0.016). 미착용군은 전자기기 사용 후 순목 횟수와 오큐튜브 검사값에서 유의한 변화를 보였고(p=0.001, p=0.000), 음주 후에는 모든 검사값에서 유의한 변화가 나타났다(p=0.039, p=0.000, p=0.000). 착용군은 전자기기 사용 후 순목 횟수(p=.034), 음주 후 오큐튜브 검사(p=0.008)에서 유의한 차이를 보였다. 건성안의 유병률은 미착용군에서 전자기기 사용 후(p=0.028), 착용군에서 음주 후 (p=0.022) 유의하게 증가하였다. 결론 : 음주와 전자기기 사용은 콘택트렌즈 착용 여부와 관계없이 대학생의 안구건조 증상을 악화시킬 수 있다.
This study was conducted from 2022 to 2024 at the Grassland and Forage Crops Division, National Institute of Animal Science (RDA), in Cheonan, Korea, to develop a medium-maturing variety of Italian ryegrass (Lolium multiflorum Lam.). The newly developed tetraploid cultivar, named ‘Spider’, is characterized by its green leaves, semi-erect growth habit in late autumn, and erect growth habit in mid-spring. With a heading date of May 16, ‘Spider’ is classified as a medium-maturing variety. Compared to the control cultivar ‘Kowinmaster’, ‘Spider’ has a 1.0 mm wider leaf blade, a 1.6 cm longer leaf blade, and is 5 cm taller in plant height. Its dry matter yield (10,169 kg/ha) is significantly higher than that of ‘Kowinmaster’ (p<0.05). The crude protein content of ‘Spider’ is 10.4%, which is 0.2% higher than that of the control. Additionally, ‘Spider’ has a neutral detergent fiber (NDF) content of 49.5% and an acid detergent fiber (ADF) content of 26.6%, showing a 2.2% lower NDF and a 0.2% higher ADF compared to ‘Kowinearly’.
본 연구는 환경 요인을 바탕으로 절화용 국화 생장 예측을 위한 최적의 모델을 개발하는 것을 목표로 하였다. 이를 위해 13개의 모델(Linear Regression, Lasso Regression, Ridge Regression, ElasticNet Regression, K-Nearest Neighbors (KNN), Support Vector Regression (SVR), Neural Network, Decision Tree, Random Forest, XGBoost, AdaBoost, CatBoost, Stacking)의 성능을 R2, MAE, RMSE를 평가 지표 로 비교하였다. 단일 모델 중에서는 Decision Tree가 가장 우수한 성능을 보였으며, R2값은 0.90에서 0.91 사이였다. 앙 상블 모델 중에서는 CatBoost가 가장 높은 성능을 보였으며 (R2=0.90~0.92) Random Forest와 XGBoost 또한 유사한 성 능을 보였다. 전체적으로 트리 기반 앙상블 모델이 국화 생장 예측에 적합한 모델로 나타났다.
The commercial feed additive, native rumen microbes (RC), derived from a diverse microbial community isolated from the rumen of Hanwoo steers is being explored to enhance rumen fermentation and improve ruminant feed utilization. This study evaluated the impact of native rumen microbes supplementation on methane emissions, microbial diversity, and fermentation efficiency on in vitro assessment. Treatments were as follows: CON (basal diet, without RC); T1 (basal diet + 0.1% RC); T2 (basal diet + 0.2% RC). Rumen fermentation parameters, total gas, and methane production were assessed at 12, 24, and 48 h of incubations. The in vitro gas production was carried out using the Ankom RF Gas Production System. Supplementation of RC significantly reduced the total gas production at 12, 24, and 48 hours of incubation (p < 0.05). Volatile fatty acid concentrations were increased, while acetate and propionate were decreased (p < 0.05) at 48 h by the supplementation of RC. Notably, the 0.1% inclusion level of RC significantly reduced methane production by 28.30% and 21.21% at 12 and 24 hours. Furthermore, microbial diversity analysis revealed significant shifts (p < 0.05) in bacterial composition between the control and treatment groups, while supplementation also promoted the growth of bacterial populations, such as Succiniclasticum. These findings suggest that native rumen microbes supplementation, particularly at 0.1% inclusion level, can enhance rumen microbial composition while significantly reducing methane production in vitro.
Oral language comprehension (OLC) and vocabulary depth are widely recognized as key predictors of reading comprehension. However, research has either provided a limited representation of OLC or failed to acknowledge the distinctive predictive nature of vocabulary depth. This study investigates the combined contributions of OLC, conceptualized as a unified composite of foundational language variables and compares its effects on reading comprehension to vocabulary depth, while accounting for writing abilities. A total of 169 advanced Korean university EFL students were tested on listening comprehension, grammar knowledge, vocabulary breadth, vocabulary depth, and reading comprehension in English. SEM analyses revealed that both OLC and vocabulary depth made unique contributions to reading comprehension, with OLC demonstrating relatively stronger predictive power. Writing abilities did not show significant effects when OLC and vocabulary depth were considered in the same model. These findings underscore the independent predictive roles of OLC and vocabulary depth in L2 reading comprehension and highlight the importance of integrating these constructs into EFL pedagogical practices.
본 연구는 저관리형 거리화단 화훼경관용 초화류 식물 선발을 위하여, 4종의 초본화훼식물(Zinnia elegans, Salvia splendens, Catharanthus roseus, Gomphrena globose )을 대상으로 무관 수 처리에 따른 내건성을 구명하고자 실시하였다. 4종 식물의 영구위조점을 알아본 결과, 무관수 후 백일초가 23일째 가장 먼저 고사하였고, 그 다음으로 살비아는 29일째, 일일초는 51일 째, 천일홍은 61일째에 고사하였다. 무관수 처리에 따른 4종의 용적수분함량 변화를 비교한 결과, 용적수분함량은 무관수 이후 지속적으로 감소하였고, 용적수분함량이 0%에 도달한 것은 백 일초가 무관수 16일째로 가장 빨랐으며, 그 다음으로 살비아 25일, 일일초 35일, 천일홍 40일 순으로 나타났다. 4종의 지상 부생체중의 감소율을 영구위조점에서 보았을 때 4종의 식물중 백일초의 감소율이 가장 높았고, 그 다음은 살비아, 천일홍, 일일초 순으로 높게 나타났다. 또한 지상부와 지하부 건물중의 증가율은 일일초가 가장 높았으며 통계적으로 유의하였다. 무관 수 처리에 따른 광합성 형광 변수 중 하나인 Fv/Fm 측정 결과, 백일초는 일시위조점인 15일째부터 감소하였고, 살비아는 무관 수 29일째, 일일초는 37일째, 천일홍은 43일째부터 감소하였 으며, 통계적으로 유의하였다. 무관수 처리에 따른 4종의 각 개체별 측정된 식생지수의 변화를 알아본 결과, 정규식생지수 (normalized difference vegetation index, NDVI), 광화학 반사율 지수(photochemical reflectance index, PRI), 안토 시아닌 반사율 지수(anthocyanin Reflectance Index, ARI) 가 무관수 기간동안 백일초, 살비아보다 일일초, 천일홍이 장기 간 건강한 잎의 범위에 속하였다. 본 연구결과를 종합해 볼 때, 저관리형 거리화단 화훼경관용 식물 선정 시 거리화단의 수분관 리 측면을 고려한다면 무관수 처리 후 약 50-60여 일간 생존하 여 내건성이 강한 것으로 나타난 일일초, 천일홍이 저관리형 거리화단 화훼경관 조성 시 적용 가능성이 매우 높은 식물로 판단되었다. 거리화단 화훼경관 조성 후 물관리가 어려운 거리 화단 화훼경관의 지속적인 유지, 관리에 대한 어려움이 부각되 어왔다. 따라서 저관리형 거리화단 화훼경관용 식물의 다양한 적용 및 관리를 위해서는 향후 보다 많은 식물종을 대상으로 한 실험뿐만 아니라 식물 내에서 품종 간의 비교에 대한 후속 연구도 필요한 것으로 생각되었다.
Fabry disease is an X-linked lysosomal storage disorder caused by GLA mutations, leading to a deficiency in α-Galactosidase A activity and subsequent accumulation of globotriaosylceramide (Gb3). This accumulation contributes to progressive multiorgan dysfunction, with cardiovascular complications, particularly endothelial dysfunction and left ventricular hypertrophy being major drivers of disease morbidity and mortality. Although enzyme replacement therapy is currently the standard treatment, its effectiveness is limited in addressing advanced cardiovascular pathology. To better understand Fabry-associated vascular and cardiac phenotypes, an isogenic human induced pluripotent stem cell (hiPSC) model in which GLA was knocked out was developed using CRISPR/ Cas9. GLA-knockout (GLA-KO) hiPSCs were differentiated into endothelial cells (ECs) and cardiomyocytes (CMs) to evaluate disease-relevant phenotypes in vitro . GLA-KO ECs exhibited normal morphology and differentiation capacity but showed markedly impaired tube formation, high expression of inflammatory genes ICAM1, VCAM1, and SELE, and increased mitochondrial and cytoplasmic reactive oxygen species levels. GLA-KO CMs demonstrated enlarged cell size and nuclear translocation of NFATC4, consistent with hypertrophic remodeling. Together, these findings recapitulate key features of Fabry vasculopathy and cardiomyopathy in a genetically defined, human-derived system. This platform enables direct investigation of Gb3-induced oxidative and inflammatory mechanisms and provides a valuable model for the preclinical evaluation of therapeutic strategies targeting the cardiovascular manifestations of Fabry disease.
본 연구는 우리나라에 자생하는 목본 관상용 식물인 먼나무 (Ilex rotunda)와 으름덩굴(Akebia quinata)을 대상으로 삽목 효율을 분석하였다. 삽수는 녹지와 숙지로 구분하고 생장조절 제 indole-3-butyric acid (IBA)와 시판용 발근촉진제 루톤 (Rootone)을 처리하여 대조군과 비교하였다. 그 결과, 발근율 (녹지, 숙지)은 먼나무(50.9, 19.0%) 및 으름덩굴(52.8, 28.5%) 로 두 수종 모두 숙지보다는 녹지가 손쉽게 발근되었다. 생장조절 제의 발근 촉진 효과는 녹지보다는 숙지에서 두드러졌으며, 생장 조절제 효과는 두 수종에서 상이하게 나타났으며, IBA와 루톤에 대한 두 수종의 발근 반응이 다르게 나타냈다. IBA 1,000ppm 처리는 먼나무의 발근을 촉진시켰고(65.9%) 으름덩굴에서는 고 사에 따른 발근율 저하를 야기하였으며(5.0%), 오히려 으름덩굴 은 루톤 처리로 발근이 촉진되었다(83.3%).
Periodontitis is a chronic inflammatory condition primarily triggered by bacterial infections, with periodontopathogens such as Porphyromonas gingivalis playing a pivotal role. We evaluated the antioxidant and anti-inflammatory effects of ethanol extract of Salvia plebeia R. Br. (SP-E) on human gingival fibroblasts (hTERT-hNOF) stimulated with P. gingivalis -derived lipopolysaccharide (LPS). Dried S. plebeia was extracted using 70% ethanol, yielding a 10.5% extract. Inflammation in hTERT-hNOF cells was induced using P. gingivalis LPS in conjunction with LPS-binding protein and CD14. SP-E was administered at concentrations ranging from 25 to 100 μg/mL. Antioxidant capacity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) activity assay. Inflammatory cytokine expression and secretion were analyzed via reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results demonstrated a concentrationdependent antioxidant effect, with 62.98% radical scavenging activity observed at 200 μg/mL SP-E. In hTERT-hNOF cells, SOD activity increased from 4.88% (LPS-treated) to 45.78% with 100 μg/mL SP-E. RT-PCR analysis showed significant downregulation of interleukin (IL)-1β, IL-6, and IL-8 mRNA expression following SP-E treatment. ELISA confirmed a reduction in tumor necrosis factor (TNF)-α (312.83 → 178.22 pg/mL), IL-6 (453.97 → 170.83 pg/mL), and IL-8 (480.14 → 276.86 pg/mL) levels with 100 μg/mL SP-E. These findings suggest that SP-E may offer therapeutic potential for preventing and managing periodontal disease by mitigating oxidative stress and modulating inflammatory cytokine expression. Further studies are warranted to elucidate the underlying molecular mechanisms and validate these effects in vivo .
Stemflow (SF) is essential for water resources within forest ecosystems and can constitute up to half of the gross rainfall (GR), depending on the forest stand structures in coniferous plantations. Although numerous studies on SF yield have been reported globally for various forest types, very few studies on SF have been reported to examine the influence of forest stand structures on SF in Korea. This study aimed to quantify the relationship between SF and forest stand structures in unmanaged Japanese cypress plantations. Two study plots were established (10 m × 10 m each) in plantations with the same stem density (SD: 2500 stems ha-1) (hereafter P1 and P2). Almost all forest stand structures (canopy projection area, tree height, diameter at breast height (DBH), number of live and dead branches, and ratio of canopy length to canopy width), including canopy volume using mobile LiDAR devices, were investigated. To evaluate the efficiency of funneling rainwater for the effect of tree biomass on SF, a funneling ratio (FR) was used. The present SF ratios (20.7% in P1 and 22.3% in P2) were much higher than those reported in previous studies of various forest types in Korea (SF ratios: 0.2–5.8% with a mean of 2.0%). This is due to the interaction between the high SD and many under-canopy dead branches. Individual-scale FR was correlated with DBH (R2 = 0.43). The present stand-scale FRs (FRstand) (22.3 in P1 and 29.2 in P2) were much higher than those reported in the previous studies (FRstand: 1.0–33.3 with a mean of 7.8) because of the negative relationship between FRstand and mean DBH (R2 = 0.78, p = 0.02). Our results provide useful information for understanding changes in SF caused by forest stand structures.
This study experimentally compares the efficacy of Situational Language Teaching (SLT) versus traditional methods in Comprehensive Chinese Language Courses (CCLC). Using an independent samples t-test for data analysis, the study confirms SLT's superior efficacy in enhancing grammatical competence and productive skills over traditional methods. The findings highlight SLT's effectiveness in creating authentic communicative contexts, fostering practical language application, and supporting skill integration. The study provides empirical evidence for adopting SLT in CCLC, particularly for grammar instruction and productive skill development, while emphasizing the importance of contextually grounded teaching practices in Chinese language education.
Three strains (KCOM 2191, KCOM 2668, and KCOM 2812) of Capnocytophaga sp. isolated from a Korean population were initially classified by 16S rDNA sequence comparison. This study aimed to resolve their species-level identity using whole genome sequencing and to assess their taxonomic characteristics. Genomes of the three strains were sequenced using PacBio RS II and Illumina platforms. Average nucleotide identity (ANI) analysis was employed for species-level identification. Cellular fatty acid (CFA) compositions were determined using the MIDI/Hewlett Packard Microbial Identification System. ANI values for KCOM 2191, KCOM 2668, and KCOM 2812 were 96.43%, 96.33%, and 96.33%, respectively, compared with the type strain Capnocytophaga ochracea DSM 7271T. CFA profiling showed a predominance of iso-C15:0 (57.9%, 67.2%, and 64.9%, respectively), consistent with DSM 7271T (51.5%). These findings confirm that KCOM 2191, KCOM 2668, and KCOM 2812 are strains of C. ochracea . These strains may serve as valuable models for investigating the role of C. ochracea in oral and systemic pathogenesis.
This study evaluated the effects of solid and water-soluble extracts derived from torrefied Pinus densiflora on lettuce (Lactuca sativa) germination and growth, as well as the cultivation efficiency of torrefied materials combined with plant nutrient solutions. Torrefied samples were prepared under various conditions (200°C, 220°C, 240°C for 20–80 minutes) and mixed with sand at ratios from 20% to 100%. Liquid extracts were formulated with macronutrients (N, P, K, Mg) and micronutrients (Mn, B, Fe, Cu) and applied to leafy and root vegetables. Germination tests showed that 60% and 80% torrefied treatments had the highest and most consistent rates, with torrefied samples promoting more rapid and stable germination than controls. A two-way ANOVA indicated a significant interaction between treatment and concentration (p = 0.043), suggesting concentration-specific effects. While shoot length showed no significant difference, root growth was marginally significant (p = 0.064), with washed torrefied material producing the longest roots. Physical analysis revealed that torrefied materials improved soil thermal retention and moisture-holding capacity. However, increasing torrefied content reduced porosity, likely due to fine particles filling soil pores. In conclusion, torrefied P. densiflora materials exhibit strong potential to enhance germination, root development, and soil condition. Optimal results were achieved with 40–60% solid mixtures for germination and 60–80% diluted extracts for rapid and consistent seedling growth. When optimally applied, they may serve as effective, sustainable amendments in cultivation systems.
This study explored the dual linguistic challenges experienced by Chinese international students enrolled in English-Medium Instruction (EMI) programs at South Korean universities. Drawing on semi-structured interviews with 20 undergraduate students from two leading private universities in Seoul, this study investigated how students navigate the linguistic demands of studying in English while simultaneously adjusting to Korean as the dominant campus language. The findings revealed that students encounter significant obstacles, including professors’ limited English proficiency, inconsistent classroom language policies, and marginalization in group work and class discussions. Despite these difficulties, many students gradually adapted through self-directed strategies and peer support, ultimately gaining academic and linguistic benefits. Some participants reported enhanced preparedness for further studies in English-speaking countries and developed bilingual competencies. The study highlights the complex realities of EMI implementation for non-Anglophone international students and calls for more transparent language policies, targeted support programs, and inclusive pedagogical practices to promote equitable learning environments.
In the context of higher education (HE) internationalization, English as a Lingua Franca (ELF) plays a crucial role in academic and interactive communication, often intersecting with local languages. This enables greater flexibility in language use and code-switching (CS) between English and the local language. While previous research on English as a Medium of Instruction (EMI) has predominantly examined the perspectives of local stakeholders, the views of international students have been largely underexplored. To bridge this gap, this study investigates the perceptions of both local and international students regarding CS practices within EMI courses across specific classroom situations. The findings reveal that both groups support the conditional use of the local language to promote comprehension while ensuring inclusivity. Local students demonstrate a broader acceptance of CS compared to their international counterparts, particularly in relation to academic outcomes such as understanding instruction and assessment. In contrast, international students show greater receptiveness to CS during interactive classroom discussions, which reflects their attitudes toward engaging with local peers. These insights contribute to the development of more nuanced EMI policies and deepen our understanding of language dynamics in HE contexts where English is not the native language.
This study explores a church leader’s perceptions of language policy goals and cultural practices within a Korean-American church, as well as the changes prompted by the COVID-19 pandemic. Drawing on theories of ethnic churches, language planning and policy, and communicative frameworks of organizational identity, the study employs critical discourse analysis and thematic analysis. Data were collected through multiple qualitative methods, including participant observation of English and Korean worship services, two in-depth interviews, audio recordings, and artifact documentation. The findings highlight the dual role of first language and cultural practices in affirming both ethnic identity and Christian faith among Korean congregants. Furthermore, this research illuminates the integrative role of the church—both institutionally and socially—during a global crisis. The church leader’s motto, “Stay well and stay connected,” was expressed through linguistic and cultural adaptations that promoted a sense of belonging and positive identity amidst uncertainty.
This study examined the effects of pre-soaking solvents and repeated steaming-drying (SD) cycles on the antioxidant activity and active compound content of Rehmanniae Radix Preparata(RRP), the processed root of Rehmannia glutinosa Libosch. SD treatments were conducted for 1 to 9 cycles using four different pre-soaking solvents: Takju (a traditional rice wine), Spirits, Honey, and Sugar solution. The results showed no significant differences in DPPH and ABTS radical scavenging activities or in total polyphenol and flavonoid contents among the pre-soaking treatments, although samples pre-soaked in honey and Takju exhibited slightly higher levels. Polyphenol and flavonoid contents increased progressively with the number of SD cycles, reaching levels 2–3 times higher after nine cycles. Catalpol content remained relatively constant regardless of treatment, whereas aucubin content increased in all groups, with the highest accumulation observed in the Takju treatment. Similarly, 5-hydroxymethylfurfural (5-HMF) content increased with the number of SD cycles, with the highest levels found in the honey group, followed by Takju, Sugar, and Spirits. In conclusion, while the type of pre-soaking solvent had minimal influence on antioxidant activities and catalpol content, both aucubin and 5-HMF contents increased with additional SD cycles, with Takju proving particularly effective in enhancing their accumulation.
Some scholars argue that after the Yuan and Ming dynasties, the function of the character "Dai"(埭) gradually diminished in real life, and the character itself slowly faded from people's view. In modern life, people may occasionally encounter the character "Dai"(埭) in some ancient place names or modern place names that inherit ancient traditions. Others suggest that the use of "Dai" (埭) in place names in Wenzhou, Zhejiang Province, is influenced by the Min dialect. However, this paper holds a different view. In fact, in the provinces and cities south of the Yangtze River, including Zhejiang, Shanghai, Jiangsu, Anhui, and Fujian, the character "Dai"(埭) is very common as a place name, with a higher frequency of use compared to its function as a water conservancy facility. It is by no means "occasional." As a frequently used place name, including in Fujian, it has not faded from people's view at all. Through diachronic examination, it is found that the character "Dai"(埭) has been used as a place name at least since before the Tang Dynasty and has never been (埭) interrupted. It was not only used as a place name after the Yuan and Ming dynasties. The toponymic use of 'Dai' (埭) across Zhejiang, Shanghai, Jiangsu, Anhui, and Fujian can be regarded as a vestige of ancient Chinese linguistic heritage. Many dictionaries, including some well-known ones, have not included the meaning of "place name character" for "Dai," which seems inappropriate. These dictionaries have included the dialectal usage of "Dai"(埭) as a measure word but have overlooked its dialectal place name usage, which is a case of "focusing on one thing while neglecting another."
This study investigates the acute toxicity of sublethal tributyltin (TBT) exposure in the marine polychaetes Perinereis aibuhitensis by measuring changes in mortality, burrowing activity, acetylcholinesterase (AChE) function, and antioxidant defense mechanisms. The 96h-LC50 of TBT was established at 23.7 μg L⁻¹. Following exposure to 1 μg L⁻¹ of TBT led to a dose-dependent reduction in burrowing behavior and AChE activity in polychaetes. Elevated levels of malondialdehyde (MDA) and pronounced reduction of glutathione (GSH) contents explained significant oxidative stress. The major antioxidant enzymes, including GSH peroxidase, GSH reductase, catalase, and superoxide dismutase, were also significantly suppressed following TBT exposure. These findings indicate that TBT-induced acute toxicity compromises physiological functions and undermines antioxidant defense system in polychates.