A female wild raccoon dog was referred with a history of generalized seizure. Mild leukocytosis was noted on laboratory tests. Gross lesions included nasal hemorrhage, hemothorax, and hemorrhage in the urinary bladder with hematuria. Microscopically, interstitial and purulent bacterial pneumonia was observed in the lungs. In the cerebellum, characteristic eosinophilic intracytoplasmic and intranuclear inclusion bodies were found in Purkinje cells, and severe demyelination was observed in the cerebellar white matter. Canine distemper virus (CDV) infection was suspected and confirmed after detection of CDV nucleoprotein RNA in the cerebrum and the lungs by nested reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Based on the histopathological and molecular diagnostic findings, it was concluded that the raccoon dog was infected with CDV.

Thirty-one Campylobacter jejuni isolates (22 from various local sources, 9 from imported chicken meats) were subtyped with PFGE and flaA typing to investigate their genetic relatedness. Based on a 90% similarity criterion, 23 and 21 genotypic patterns were formed by PFGE and flaA typing, respectively. The discriminatory indices for PFGE, flaA typing, and a composite analysis of PFGE and flaA typing were 0.9785, 0.9527, and 0.9871, respectively. Similar patterns in composite analysis were observed between sources (cattle and chicken, and cattle and human), indicating that reservoir animals may have been the source of human campylobacteriosis. Therefore, strict hygiene measures from farm to table should be implemented to prevent diseases due to C. jejuni in humans.

The objective of this study was to investigate the efficiency of nicotinic acid on sperm cryosurvival and fertilization ability in frozen-thawed boar semen. Boar semen was collected by glove-hand method and was frozen using freezing solution treated to 0, 5, 10 and 20 mM of nicotinic acid. The frozen sperm for sperm characteristic analysis was thawed such as viability, acrosome reaction, and mitochondrial integrity. The frozen-thawed sperm was estimated by SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction and Rhodamine123/PI double staining for mitochondrial integrity using a flow cytometry. The embryo was estimated in vitro development and DCFDA staining for reactive oxygen species assessment. As results, frozen-thawed sperm viability was significantly higher in 5 and 10 mM (61.1 ± 1.5%, 64.7 ± 2.0%) of nicotinic acid than other groups (0 mM, 52.1 ± 2.3%; 20 mM, 47.8 ± 5.1%, P<0.05). The live sperm with acrosome reaction was significantly higher in 5 and 10 mM of nicotinic acid (26.1 ± 1.8%, 24.9 ± 1.5%) than other groups (0 mM, 35.3 ± 0.8%; 20 mM, 36.5 ± 1.9%, P<0.05). The live sperm with mitochondrial integrity was significantly higher in 5 and 10 mM (84.2 ± 3.6%, 88.4 ± 2.3%) of nicotinic acid than other groups (0 mM, 77.3 ± 4.4%; 20 mM, 73.3 ± 3.6%, P<0.05). Blastocyst rate of in vitro development was significantly higher in 10 mM (17.0 ± 1.3%) of nicotinic acid than other groups (0 mM, 9.4 ± 0.5%; 5mM, 12.6 ± 0.8%; 20 mM, 5.0 ± 1.0%, P<0.05). Moreover, total cell number was higher in 5 and 10 mM (53.6 ± 2.9%, 57.9 ± 2.8%) of nicotinic acid than other groups (0 mM, 41.0 ± 1.4%; 20 mM, 23.2 ± 2.8%, P<0.05). Hydrogen peroxide in embryos was lower in 5 mM nicotinic acid (0.7 ± 0.1%) than other groups (0 mM, 1.0 ± 0.1%; 10mM, 0.9 ± 0.0%; 20 mM, 1.4 ± 1.0%, P<0.05). In conclusion, nicotinic acid-treated semen improves cryosurvival and quality of spermatozoa. Also, the fertilized oocytes with nicotinic acid improve quality of embryo and blastocyst formation.

Cubic mesocrystal CeO2 was synthesized via a hydrothermal method with glutamic acid (C5H9NO4) as a template. The XRD pattern of a calcined sample shows the face-centered cubic fluorite structure of ceria. Transmission electron microscopy (TEM) and the selected-area electron diffraction (SAED) pattern revealed that the submicron cubic mesocrystals were composed of many small crystals attached to each other with the same orientation. The UV-visible adsorption spectrum exhibited the red-shift phenomenon of mesocrystal CeO2 compared to commercial CeO2 particles; thus, the prepared materials show tremendous potential to degrade organic dyes under visible light illumination . With a concentration of a rhodamine B solution of 20 mg/L and a catalyst amount of 0.1 g/L, the reaction showed higher photocatalytic performance following irradiation with a xenon lamp (≥ 380 nm). The decoloring rate can exceed 100% after 300 min.

Prolonged sitting can contribute to low back pain. The lumbar taping can be applied to correct the sitting posture. This study aimed to investigate the effect of lumbar taping on lumbar kinematics and the muscle activities of multifidus (MF) and internal oblique in the individuals with nonspecific chronic low back pain (NSCLBP) as they type for 30 minutes. Nineteen subjects with NSCLBP (9 people in non taping group and 10 people in taping group) were recruited. Lumbar taping was applied to the taping group before typing. Both groups started typing in a neutral sitting position with their feet on the floor. The change of posture and S2 posterior tilting (S2P) were measured to investigate kinematic data. Three sensors were attached on T12, L3, and S2 to identify the change of posture. Surface electromyography was used to measure the muscle activities. Palpation meter was used to standardize the angle of pelvic tilt in sagittal plane before typing. All instruments were used to measure each data before and after typing. Independent t-test was used to compare the changing values of lumbar kinematics and muscle activities before and after typing between both groups. The changing values of S2P and change of posture of L3 and S2 were significantly smaller in the taping group compared to the non taping group (p＜05). The changing value of muscle activities of MF between before and after typing was significantly smaller in the taping group compared to the non taping group (p＜05). In conclusion, the lumbar taping during the 30-minute typing task can be applied to maintain correct sitting posture in the lumbar and pelvis and to maintain activation of MF.

The effects of Proprioceptive Neuromuscular Facilitation (PNF) and elastic band exercise on the physical functions and blood lipids of obese elderly women were investigated. The experimental group (n1=16) patients underwent PNF for 12 weeks, and the control group (n2=15) patients performed elastic band exercises. SPSS 21.0 was used to compute the means and standard deviations. After the 12-week PNF, both the experimental and control groups showed statistically significant differences in the physical functions (cardiovascular endurance, strength of the lower extremity, muscular endurance, flexibility, balance, and agility) (p<.05), but the difference in the experimental group was more significant than that in the control group (p<.05). In terms of the changes in the blood lipid levels (total cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein), the experimental group showed significant changes (p<.05). In conclusion, PNF was confirmed as more effective than elastic band exercise in improving the physical functions and blood lipid levels of obese elderly women.

Carbon aerogel is a porous carbon material possessing high porosity and high specific surface area. Nitrogen doping reduced the specific surface area and micropores, but it furnished basic sites to improve the CO2 selectivity. In this work, N-doped carbon aerogels were prepared with different ratios of resorcinol/melamine by using the sol-gel method. The morphological properties were characterized by scanning electron microscopy (SEM). Nitrogen content was studied by X-ray photoelectron spectroscopy (XPS) and the specific surface area and micropore volume were analyzed by N2 adsorption-desorption isotherms at 77 K. The CO2 adsorption capacity was investigated by CO2 adsorption-desorption isotherms at 298 K and 1 bar. Melamine containing N-doped CAs showed a high nitrogen content (5.54 wt.%). The prepared N-doped CAs exhibited a high CO2 capture capacity of 118.77 mg/g (at resorcinol/melamine = 1:0.3). Therefore, we confirmed that the CO2 adsorption capacity was strongly affected by the nitrogen moieties.

This study is to explore the relationships between sensibility images of mannequins and apparel shops. A total of 113 consumers participated in experiments with photo stimuli of 2 mannequin types (realistic and semi-abstract mannequins) and 4 brand shops of women's casual wear. In results, luxurious, chic, strong, sexy, and young images were more strongly perceived from realistic mannequins than semi-abstract mannequins whereas simple and soft images were more strongly perceived from semi-abstract mannequins than realistic mannequins. Shops using realistic mannequins indicated strong images whereas shops using semi-abstract mannequins presented soft, comfortable, and feminine images. In the correlation analysis, luxurious, chic, strong, and young images of realistic mannequins were consistent with shop images using realistic mannequins. Also, luxurious, chic, soft, comfortable, and feminine images of semi-abstract mannequins were consistent with shop images using semi-abstract mannequins. In order to clearly communicate brand concepts with consumers, mannequin that is a key element of visual merchandising in the apparel shop, should be carefully selected, considering the accordance with shop image.

신뢰성 해석을 수행할 때 정보부족으로 인해 발생하는 인식론적 불확실성(epistemic uncertainty)은 고유의 변동성에 의해 존재하는 내재적 불확실성(aleatory uncertainty)보다 더 중요하게 다뤄야 한다. 그러나 그동안 개발된 확률이론은 주로 내재적 불확실성을 모델링하는데 이용된 반면, 인식론적 불확실성의 모델링에 대해서는 아직 확실한 접근법이 없었다. 최근 이를 위해 probability theory를 포함한 여러 접근법들이 제시되고 있지만 이들은 서로 다른 통계적 이론들을 바탕으로 도출되었기 때문에, 각 방법들의 결과들을 이해하는데 어려움이 있었다. 본 연구에서는 고장 확률을 계산하는 문제를 가지고 이러한 방법들이 인식론적 불확실성을 어떻게 다루는지를 비교, 분석하였다. 이를 위해 probability method, combined distribution method, interval analysis method 그리고 evidence theory를 대상으로 신뢰도 분석문제에 대해 각 방법들의 특징들을 비교하였으며, 그 결과는 다음과 같다. 입력변수의 확률분포 형태를 알 수 있다면 probability method가 가장 우수하나, 이를 전혀 모르면 interval method를 사용해야 한다. 그러나 계산비용 면에서는 두 방법이 유사하므로 결국 입력변수의 확률특성 정보가 얼마나 있느냐에 따라 방법을 선택한다. Combined distribution method는 failure probability의 평균만 제공하므로 사용하지 않는 것이 좋다. 다만 이 방법은 계산비용이 매우 적게 드는 장점이 있다. Evidence theory는 probability와 interval 방법의 중간에 해당하며, 구간별 probability assignment를 세분화 할수록 probability결과에 접근한다. 이 방법은 계산비용이 가장 높은 것이 문제이다.

자연재해에 있어서 취약성 분석계획의 우선순위 설정활동을 위한 기초자료로서 재해 위험분석의 주요한 요소이다. 화산폭발은 화산이 인구밀도가 높은 지역에 위치하고 있는 경우 많은 사상자와 재산피해를 야기한다. 화산폭발을 막을 수 없지만, 위험도와 취약성은 미래의 위기를 예측하는 신중한 계획과 준비작업을 통해 저감될 수 있다. 사회적 불평등으로서 사회적 취약성은 다양한 사회 구성원이 재해에 대응 능력에 민감한 영향을 미친다. 본 연구에서는 인도네시아 중부 자바에 위치한 메라피 화산 인근 지역주민들의 사회적 취약성을 평가하기 위하여 사회적 취약성 지수(SoVI) 기법을 활용하였다. SoVI는 사회적 위험부담을 이해하고 정량화하기 위하여 지역사회의 복원탄력성에 영향을 미치는 사회 경제적 및 인구 통계학적 요인을 이용하여 평가된다. 본 연구에서 사회적 취약성은 이해 관계자와 지역주민과의 설문조사를 통해 평가되었다. 연구결과로 도출된 취약성 지수는 사회적 취약성의 분포와 원인에 대한 정보를 반영함을 확인하였다.

칼데라는 저장되어 있는 마그마의 대규모 폭발적인 분화 다음에 발생하는 지반의 붕괴에 의해 형성된다. 지구상에서 칼데라는 수 킬로미터에서 수십 킬로미터의 크기에 이르는 다양성을 갖는다. 칼데라 붕괴에 관련된 인도네시아의 화산붕괴는 많은 사망자뿐만 아니라 전 지구적 영향을 미친 바 있다. 본 연구는 인도네시아 칼데라의 사례 연구를 통해 칼데라인 백두산과의 유상성을 분석하였다. 주요 분석 관점은 주요 위해 요인, 최근 화산활동의 증상 및 가까운 장래에 분화하는 경우의 위험성 등이며, Krakatau산, Tambora산, Ijen산, Tengger 칼데라, Rinjani산 및 Ranau 칼데라에 대한 가중 평가 매트릭스를 사용하여 유사성 분석을 수행하였다.

Residential thermal conditions are important because people spend the majority of their time in the home environment. Indoor temperature and relative humidity(RH) were measured continuously over 1 year in 14 residences in Seoul, Korea. The relationship between residential indoor and outdoor conditions were determined by four meteorological parameters-temperature, apparent temperature(AT), RH, and absolute humidity(AH). Outdoor and indoor temperature, AT and AH were closely correlated, but RH was not. While indoor temperatures, AT, and AH were significantly higher than the corresponding outdoor levels, indoor RH was significantly lower than outdoor RH. Regression models between indoor and outdoor temperature detected a heating threshold at 15.0oC of outdoor temperature. The indoor thermal conditions were significantly different by the two residence types. Indoor temperatures in apartments were lower in summer and higher in winter than those in detached houses. However, indoor RHs in apartments were lower than in detached houses. During tropical nights, the daily temperature range was higher in residences with air-conditioning than in naturally ventilated residences.

차 중에 있는 살균제 tridemorph의 잔류량을 검사하기 위해 LC-ESI-MS/MS를 이용한 정확하고 감도가 좋은 분석방 법을 개발하였다. Tridemorph 잔류물은 샘플을 수화한 후 acetonitrile로 추출하고, NaCl을 이용한 액-액 분배, NH2 카트리지 정제를 거쳐 기기분석을 수행하였다. 직선성은 0.02~1.0 μgmL−1 범위에서 상관계수(r2) 0.9999로 높은 직선 성을 보였다. 0.02와 0.05 mgkg−1 처리수준으로 회수율을 실험한 결과는 75.0~84.7% 이었으며, 상대표준편차는 10% 미만이었다. 분석방법의 검출한계와 정량한계는 각각 0.01 와 0.02 mgL−1 이었다. 이러한 결과들을 통해 확립된 분석법은 차 중 tridemorph의 잔류량 분석에 적합함을 확인할 수 있었다.

농산물 중에 있는 제초제 saflufenacil의 잔류량을 검사 하기 위해 HPLC-UVD와 LC-MS를 이용한 정확하고 감도가 좋은 분석방법을 개발하였다. Saflufenacil 잔류물은 acetone 추출, dichloromethane을 이용한 액-액 분배, silica 와 carbon 카트리지 정제를 거쳐 기기분석을 수행하였다. 검량선 작성을 위해 0.1~5.0 μgmL−1 범위로 표준품을 만들어 실험한 결과 상관계수(r2)는 0.999로 높은 직선성을 보였다. 0.02~0.5 mg kg−1 처리수준으로 회수율을 실험한 결 과는 80.5~110.2% 이었으며, 상대표준편차는 10% 미만이었다. 분석방법의 검출한계와 정량한계는 각각 0.005와 0.02 mg L−1 이었다. 확립된 시험법으로 본청, 부산지방식품의 약품안전청과 경인지방식품의약품안전청에서 실험실간 검증을 실시한 결과 만족스런 결과를 얻었다. 이러한 결과 들을 통해 확립된 시험법은 농산물 중 saflufenacil의 잔류 량 분석에 적합함을 확인할 수 있었다.

Intensity interferometry, based on the Hanbury Brown–Twiss effect, is a simple and inexpensive method for optical interferometry at microarcsecond angular resolutions; its use in astronomy was abandoned in the 1970s because of low sensitivity. Motivated by recent technical developments, we argue that the sensitivity of large modern intensity interferometers can be improved by factors up to approximately 25 000, corresponding to 11 photometric magnitudes, compared to the pioneering Narrabri Stellar Interferometer. This is made possible by (i) using avalanche photodiodes (APD) as light detectors, (ii) distributing the light received from the source over multiple independent spectral channels, and (iii) use of arrays composed of multiple large light collectors. Our approach permits the construction of large (with baselines ranging from few kilometers to intercontinental distances) optical interferometers at the cost of (very) long-baseline radio interferometers. Realistic intensity interferometer designs are able to achieve limiting R-band magnitudes as good as mR ≈ 14, sufficient for spatially resolved observations of main-sequence O-type stars in the Magellanic Clouds. Multi-channel intensity interferometers can address a wide variety of science cases: (i) linear radii, effective temperatures, and luminosities of stars, via direct measurements of stellar angular sizes; (ii) mass–radius relationships of compact stellar remnants, via direct measurements of the angular sizes of white dwarfs; (iii) stellar rotation, via observations of rotation flattening and surface gravity darkening; (iv) stellar convection and the interaction of stellar photospheres and magnetic fields, via observations of dark and bright starspots; (v) the structure and evolution of multiple stars, via mapping of the companion stars and of accretion flows in interacting binaries; (vi) direct measurements of interstellar distances, derived from angular diameters of stars or via the interferometric Baade–Wesselink method; (vii) the physics of gas accretion onto supermassive black holes, via resolved observations of the central engines of luminous active galactic nuclei; and (viii) calibration of amplitude interferometers by providing a sample of calibrator stars.

Optimum lot size calculation for real world manufacturing environment has been focused since last few decades. Several extensions have been made to the basic economic order and production order quantity models to realize the possible practical situations in industry. However, focus on work-in-process inventory has been ignored relatively. This paper provides a comprehensive review of the models developed for group technology based manufacturing environment focusing on work-in-process inventory. Models have been extended from a perfect manufacturing conditions to an imperfect manufacturing situation considering rework, rejection and inspection. Optimum lot size has been evaluated using a simple algebraic optimization approach. Significant parameters are highlighted using sensitivity analysis for the developed models. Numerical example is used to illustrate the utilization of such models in day-to-day production setups and the impact of significant factors’ variation on total cost and optimum lot size.

Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the efficiency of transfection needs to be improved. In the present study, three transfection protocols - non-liposomal transfection (NLT), magnetofection and electroporation - were compared by analysis of their transfection efficiencies and cell viabilities using human dental pulp cells (hDPC) and bovine fetal fibroblasts (bFF) as cell sources. Enhanced green fluorescent protein gene was used as the delivery indicator. For magnetofection, Polymag reagent was administrated. NLT, FuGENE-HD and X-treme GENE 9 DNA transfection reagents were used for NLT. For electroporation, the NeonTM and NEPA21TM electroporators were tested. NeonTM electroporation showed highest transfection efficiency when compared with NLT, magnetofection, and NEPA21TM electroporation, with transfection efficiency of about 33% in hDPC and 50% in bFF, based on viable cell population in each cell type. These results suggest that transfection by NeonTM electroporation can be used to deliver foreign genes efficiently in human and bovine somatic cells.

Over the last 30 years the extent of sea-ice covering the North Pole has declined about 15∼20% on average, and it is expected that by the end of the 21st century, vast areas of the Arctic Ocean will be ice-free during the summer season, thus, increasing the possibility of ship navigation across the Arctic Ocean. In this paper, we have developed a model of shipping for the Northwest Passage (hereinafter NWP) in northern Canada to determine whether ice thinning will make this route more economically viable when compared with the Panama Canal. Among the 7 routes of the NWP, Route 2 and Route 3 offer better navigational conditions than the others. However, Route 3 has a depth limit of only 10m, so here we use Route 2 which is suitable for deep draft navigation to carry on our analysis. Container ships of 4,500∼15,000 TEU under Hub and spoke mode, container shipping between Busan port and New York port is simulated for the Panama Canal and the NWP. This paper considers Canada’s sovereignty in matters of navigation over the waters at the Arctic Archipelago, in the form of a toll fee for passage usage. We concluded that the NWP has an advantage over the Panama Canal if it is open for free international passage, regardless of ship size. However, if it is not free, its advantages depend on its toll fee. The lower the toll fee is, the more advantages the NWP will boast.

A 6-year old, Greyhound bitch was presented with vaginal hemorrhage and dystocia. Physical examination revealed severe vaginal hemorrhage, abdominal pain, pale mucous membranes and the presence of solid structures to abdominal palpation. A hematological test revealed a marked hemorrhagic anemia, and abdominal radiography and ultrasonographic examination showed two dead fetuses in the uterus. Median laparotomy revealed a rupture of the left uterine horn adjacent to the bifurcation, region of weakened uterine wall in the right uterine horn, blood clots and uterine fluids in abdominal cavity without septic peritonitis. The bitch underwent ovariohysterectomy and recovered without complication.

Klotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing β-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous study has shown that transient receptor potential vanilloid type 6 (TRPV6), a calcium ion transporter, is predominantly expressed in the uterine endometrium during pregnancy in pigs. However, expression and function of KL in the uterine endometrium has not been determined in pigs. Thus, the present study determined expression and regulation of KL in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that levels of KL mRNA decreased between Days 12 to 15 of the estrous cycle, and its expression showed a biphasic manner during pregnancy. KL mRNA was expressed in conceptuses and in chorioallantoic tissues during pregnancy. Explant culture study showed that expression levels of KL were not affected by treatment of steroid hormones or interleukin-1beta during the implantation period. Furthermore, levels of KL mRNA in the uterine endometrium from gilts carrying somatic cell nuclear transfer (SCNT)- derived embryos were significantly lower than those from gilts carrying natural mating-derived embryos on Day 12 of pregnancy. These results exhibited that KL was expressed at the maternal-conceptus interface in a pregnancy statusand stage-specific manner, and its expression was affected by SCNT procedure, suggesting that KL may play an important role in the establishment and maintenance of pregnancy in pigs.