The lyophilization of the solution extracted from 60 percent of acetone applied to persimmon leaves, the compounding process in accordance with the solution's concentration, and the gel filteration through Sephadex G-50 of biologically activated substances obstructing enzyme activity, such as tyrosinase, xanthine oxidase, and angiotesin converting enzyme (ACE) led to the assumption that polyphenol was the compound serving as biologically activated substances obstructing enzyme activity. Xanthine oxidase involved in pruine metabolism oxidizes hypoxanthine to xanthine and xanthine to uric acid. In the continuous study for natural compound, nine flavan-3-ols have been isolated from the persimmon leaves. The structures of (+)-catechin, (+)-gallocatechin, procyanidin B-1, pyrocyanidin C-1, prodelphinidin B-3, gallocatechin-(4α→8)-catechin, procyanidin B-7-3-O-gallate, procyanidin C-1-3'-3'-3'-O-trigallate and (-)-epigallocatechin-(4α→8)-epigallocatechin-(4α→8)-catechin were established by NMR and their inhibitory effect on xanthine oxidase activity was investigated. Procyanidin B-7-3-O-gallate, (-)-epigallocatechin-(4α→8)-epigallocatechin-(4α→8)-catechin and procyanidin C-1-3'-3'-3'-O-trigallate showed 94%, 90.69%, 80.90% inhibition at 100 (μ)M and inhibited on the angiotensin converting enzyme respectively. Procyanidin B-7-3-O-gallate and procyanidin C-1-3'-3'-3'-O-trigallate showed 66%, 63% inhibition at 100 (μ)M and inhibited on the xanthine oxidase competitively. Procyanidin C-1-3'-3'-3'-O-trigallate showed 70% inhibition at 100 (μ)M and inhibited on the tyrosinase competitively.

The purpose of this study is the fabrication of nano-sized Fe-Co alloy powders with soft magnetic properties by the slurry mixing and hydrogen reduction (SMHR) process. 0 and powders with 99.9% purities were used for synthesizing nanostructured Fe-Co alloy powder. Nano-sized Fe-Co alloy powders were successfully fabricated using SMHR, which was performed at 50 for 1 h in H atmosphere. The fabricated Fe-Co alloy powders showed ' phase (ordered body centered cubic) with the average particle size of 45 nm. The SMHR powder exhibited low coercivity force of 32.5 Oe and saturation magnetization of 214 emu/g.

Two-component ceramic (alumina-zirconia) composites were fabricated by a soft-solution process in which polyethylene glycol (PEG) was used as a polymeric carrier. Metal salts and PEG were dissolved in ethyl alcohol without any precipitation in 1:1 volume ratio of alumina and zirconia. In the non-aqueous system, the flammable solvent made explosive, exothermic reaction during drying process. The reaction resulted in formation of volume expanded, porous precursor powders by a vigorous decomposition of organic components in the precursor sol. The PEG content affected the grain size of sintered composites as well as the morphology of precursor powders. The difference of microstructure in sintered composite was attribute to the solubility and homogeneity of metal cations in precursor sol. At the optimum amount of the PEG polymer, the metal ions were dispersed effectively in solution and a homogeneous polymeric network was formed. It made less agglomerated particles in the precursor sol and affected on uniform grain size in sintered composite.

본 논문의 목적은 빔 구조물에서 발생할 수 있는 손상의 위치를 탐색하고, 그 손상의 정도를 추정할 수 있는 알고리즘을 제안하는 것이다. 제안된 방법은 구조물의 모달 변형에너지의 차이를 이용한다. 구조물 내 발생한 국부적인 손상의 위치를 파악하고 그에 상응하는 손상도를 추정할 수 있는 손상지수를 손상 전과 손상 후 구조물의 모드형상에서 얻을 수 있는 모달 변위로 표현하였고 그 관계식을 정립하였다. 구조물 내 손상의 위치를 결정하는 방법은 기 개발된 손상 지표를 적용하였다. 제안된 방법의 우수성과 효용성은 수치적으로 손상을 모사한 빔 구조물을 이용하여 입증하였다.

지진취약도분석 기술은 원자력발전소의 구조물 및 기기의 실제 내진성능을 평가하기 위하여 이용된다. 이 논문에서는 원자력 발전소를 구성하는 구조물들의 지진취약도를 평가하는 개선된 기법에 대하여 요약하였다. 또한, 최근 몇 년간 한반도에서 발생된 소규모 기록지진의 응답스펙트럼에 대한 공학적 특성을 평가하고, 이러한 기록지진의 응답스펙트럼을 부지의 실제 지반운동으로 사용할 경우, 지진취약도 분석에 미치는 영향을 검토하였다. 몇가지 예제 구조물에 대한 지진취약도분석을 통하여 기록지진의 특성이 한국형 원자력발전소의 내진성능에 미치는 영향을 정량적으로 평가하였다. 평가결과, 현재까지 부지의 실제 지반운동으로 사용되어 오던 Newmark 스펙트럼은 국내 시설물의 내진성능을 과대평가 할 수 있음을 보여주었다.

본 연구에서는 연속체해석을 토대로, 병렬코아를 갖는 아웃리거구조물의 수평하중에 대한 응력과 변위를 구하기 위한 방법을 제안하였고, 아웃리거의 위치에 따른 구조물의 거동에 대해 연구하였다. 아웃리거와 코아가 만나는 위치에서 코아의 회전병위가 아웃리거의 회전변위와 일치한다는 적합조건으로부터 아웃리거의 구속모멘트, 코아의 휨모멘트, 기둥의 축방향력, 그리고 구조물의 수평변위 등을 유도하였다. 구조모델들을 이용하여 MIDAS-GEN 프로그램에 의한 결과와 비교하였고, 병렬코아를 갖는 아웃리거구조물에 대해 만족할만한 결과를 얻었다. 구조물 최상단의 수평변위는 코아의 위치보다는 아웃리거의 위치에 의해 크게 영향을 받는 것으로 조사되었다. 비록, 이 논문에서 제시된 공식들이 이상적인 아웃리거구조물에 대한 것이지만 병렬코아를 갖는 실제 아웃리거구조물의 응력과 변위의 근사치를 추정하고, 구조물의 거동을 예측하기 위한 수단으로 유용하게 사용될 수 있을 것이다.

Co Evolutionary Structural Design(CESD) Framework is presented, which can deal with the load design and structural topology design simultaneously. The load design here is the exploration algorithm that finds the critical load patterns of the given structure. In general, the load pattern is a crucial factor in determining the structural topology and being selected from the experts어 intuition and experience. However, if any of the critical load patterns would be excluded during the process of problem formation, the solution structure might show inadequate performance under the load pattern. Otherwise if some reinforcement method such as safety factor method would be utilized, the solution structure could result in inefficient conservativeness. On the other hand, the CESD has the ability of automatically finding the most critical load patterns and can help the structural solution evolve into the robust design. The CESD is made up of a load design discipline and a structural topology design discipline both of which have the fully coupled relation each other. This coupling is resolved iteratively until the resultant solution can resist against all the possible load patterns and both disciplines evolve into the solution structure with the mutual help or competition. To verify the usefulness of this approach, the 10 bar truss and the jacket type offshore structure are presented. SORA(Sequential Optimization & Reliability Assessment) is adopted in CESD as a probabilistic optimization methodology, and its usefulness in decreasing the computational cost is verified also.

본 논문에서는 유연한 철제와 목조 지붕구조체를 가진 1/2 축소 전단벽 건물의 진동대 실험결과를 이용한 성공적인 구조물의 재해석과 선형ㆍ비선형 동적특성을 연구하기 위한 해석모델링 방법에 대해서 연구하였다. 대상 건물은 유연한 지붕구조체와 4개의 보강조적조 전단벽으로 구성되어 있다. 유연한 지붕구조체의 동적특성 때문에 다자유도 모델을 사용하였으며, 다수의 선형 및 비선형 동적해석을 실행하기 위하여 구조물의 자유도를 단순화하였다. 각각의 구조부재의 이력특성과 재료특성 그리고 동적해석을 수행하기 위한 주요 구조변수의 적절한 설정 방법에 대하여 고찰하였다.

IASL(iodo acetamide spin label) and MSL(maleimide spin label) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. The muscle was isometrically tetanized with three trains of 3ms pulses every 50ms between 5℃ with 25℃. Equatorial reflection change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of 143a and 72a could offer information of the mass projection of population of myosin head along the filament axis. The slope of intensity profile of the mass projection of 143a and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of 215a reflection intensity the periodical character of 143a reflection by spin label. The raise of MSL actin reflection at 51a and 59a in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we can conclude that IASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.

본 연구는 충남 금산군에 위치한 진악산 산림식생을 대상으로 식물사회학적 방법으로 산림군락을 분류하고 방형구법으로 임분 특성을 분석하였다. 진악산의 26개 조사구를 분석한 결과, 산림군락은 신갈나무군락, 굴참나무군락, 갈참나무군락 및 소나무군락으로 구분되었으며, 피도율은 교목층이 79.4%, 아교목층이 27.6%, 관목층이 37.0%, 초본층이 31.1%의 순으로 나타났다. DBH 2 cm이상의 수목을 대상으로 중요치를 분석한 결과는 신갈나무가 45.51

be involved in the development of oral SCC. If we compare the morphologic features of NHOK to IHOK according to calcium concentration by TEM, IHOK have been gained wide acceptance as a model system for HPV-linked oral carcinogenesis. We already have established immortalized oral keratinocytes(IHOK) transfected by E6 and E7 gene. The purpose of this study were to examined the ultrastructural features of cultured NHOK, IHOK, and HN4 oral squamous cell carcinoma cell line, and to apply these results to oral carcinogenesis in the future. NHOK from healthy retromolar pad was primarily cultured at 37oC and 5% CO2. IHOK, and HN 4 cell line which were cultured under 0.15 and 1.2mM Ca++ of KBM bullet kit. For transmission electronmicroscopy(TEM), under preconfluency, and after 3 days of postconfluency under 1.2mM Ca++, cultured NHOK, IHOK, and HN4 cell line were immediately fixed in 2.0% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4OC for 1h. The ultrathin sections were stained with uranyl acetate and lead citrate, and examined by TEM. The obtained results were as follows. 1. TEM of cultured NHOK under 1.2mM Ca++ showed increased tonofilaments, and vaculated ovoid cells with cornified envelope, while cultured IHOK showed prominent microvilli, unilateral desmosome in microvillus, and tonofilaments. 2. TEM of HN 4 cell line sowed numerous microvilli, increased N/C ratio, and lateral desmosome in microvilli under 0.15mm, while under 1.2mM well forming desmosomes. From the aboving results, under high calcium cultured IHOK showed less tonofilaments than that of cultured NHOK, while cultured IHOK, and HN 4 cell lines showed more increased desmosomes under high calcium. It was suggested that the ultrastructural changes of cultured IHOK would be accepted as intermediate stage cells for studying oral carcinogenesis.

Tension members is a type of effective structural member, which is often used in large span structures. The structure systems composed with tension members are combined in various way and specific formations. So, there are need to research into the formations of tension structure and the type of adaptation in tension structure architectures. The structure systems with tension members were considered as tension main system, vector system and tension supported bending system, comprehensively. And tension structures were classified into the formation of tension structure with uniaxial or multiaxial line tension member, with surface member, with hybrid member of line and surface, concerning the flow of tension force. In each the formation of tension structure, the typical adaptations to architecture were also investigated through architecture examples. The type of the formation can be used to plan an architecture with respect to the flow of tension force and structural feature.

The purpose of this study is to obtain the optimum shape of cable domes by using the real coding genetic algorithm. Generally, the structural performance of the cable domes is influenced very sensitively by pre-stress, geometry and length of the mast because of flexible system. So, it is very important to decide the optimum shape to get maximum stiffness of cable domes. We use the analytical model to verify the usefulness of this algorithm for shape optimization and analyze the roof system of Seoul olympic gymnastic arena as analytical model of a practical structures. It is confirmed lastly that the optimum shape domes have more stiffness than initial shape ones.

The purpose of this study is to improve convergence speed of topology optimization procedure using the existing ESO method and to deal with topology decision of the truss structures according to a boundary condition, such as cantilever type. At the existing ESO topology optimization procedure for the truss structures, the adjustment of member sizes according to target stress has been executed by increasing or reducing a very small value from each member size. In this case, it takes too much iteration till convergence. Accordingly, it is practically hard to obtain optimum topology for a large scale structures. For that reason, it is necessary to improve convergence speed of ESO method more effectively. During the topology decision procedure, member sizes are adjusted by calculating approximate solution for member sizes corresponding to the target stress at every step, the new member sizes are adjusted by such method are applied in FEA procedure of next step.

The cut tree approach of Montreuil and Ratliff [16] and eigenvector approach [10] are used to automatically draw a feasible facility layout with aisle structure. The department arrangement can minimize an aisle distance criterion considering door location