모유에 존재하는 유지방구의 막을 구성하는 주된 당단백질인 하나인 lactadherin(과거에는 BA46로 일컬어짐)은 rotavirus에 의한 감염증상을 예방하는 것으로 보고되고 있다. 본 연구에서는 retrovirus vector system을 이용하여 Chinese Hamster Ovary (CHO) 세포에 tetracycline에 의해 발현이 제어되는 promoter 하의 lactadherin 유전자를 전이 시킨 후 lactadherin이 tetracycline에 의해 발현이 유도되는지의 여부를 실험하였다. 먼저 기초 실험으로 대장균의 LacZ 유전자를 이용하여 tetracycline에 의한 유도 여부를 시험하였다. RevTet-On과 RevTRE-LacZ retrovirus를 동시감염시킨 NIH3T3는 doxycycline (tetracycline 유도체)에 의해 투여량에 비례하여 반응정도가 증가하는 양상을 나타내었으며 최대의 반응은 doxycycline 농도가 1 ㎍/ml 이상에서부터 관찰되었다. 이 예비실험의 결과를 바탕으로 RevTet-On과 RevTRE-Ltd retrovirus vectyor를 이용하여 사람의 lactadherin 유전자의 유도적 발현을 검정하였는데 CHO 세포에서 lactadherin 유전자의 유도적 발현을 RT-PCR 기법을 이용하여 확인하였다. 표적세포 내에서 외부에서 도입된 유전자가 지속적으로 발현될 경우 심각한 생리적 부작용을 야기시킨다는 사실을 감안할 때 본 실험의 결과는 유전자 치료와 형질전환동물의 생산에 크게 도움이 될 것으로 예상된다.

For the safety extirpation in case of acute pulpitis with painless procedure, lots of pulp devitalizers have been designed for a long time. Authors have manufactured experimental pulp devitalizer with adding some other constituents for main components of paraformaldehyde and local anesthetics. The purpose of this study was to observe the histopathologic tissue response in pulp tissues by applying newly developed safety pulp devitalizer to the human tooth. For this experiments 5 human teeth in 4 patients (ages of 26 to 49) were used. In case of acute pulpitis with pulp exposure, divided into two groups; One group, carious dentin removed, and then was applied newly developed devitalizer directly to exposed small pulp tissues. Other group, was exposed the pulp chamber, pulpotomized and then the devitalizer was applied to remaining pulp tissues. The teeth were extracted on 3rd and 7th day after operation respectively. For the control group, Depulpin? was used as pulp devitalizer. All the extracted teeth were fixed in 10% neutral formalin solution, decalcified in Plank-Richlo solution, embedded in paraffin, sectioned 6-8㎛ in thickness, stained with hematoxylin-eosin stain method, and examined under microscope. Attained results were as follows; 1. There were no difference in histopathologic aspects either exposed or not pulp chamber, the extent of necrosis of pulp tissue and destruction of odontoblast was similar to that on the 3rd day of control group and both the 3rd and 7th day of the experimental group. 2. For the necrosis of pulp tissue and destruction of odontoblast on the 3rd day of control group exposed pulp chamber, was similar to that on the 3rd day of experimental group exposed small pulp tissue. The experimental pulp devitalizer could reduce the side effects, which reduced the amount of paraformaldehyde components, adding other constituents, reduced the duration of pulp devitalization, and useful whether exposed or not the pulp chamber.

be involved in the development of oral SCC. If we compare the morphologic features of NHOK to IHOK according to calcium concentration by TEM, IHOK have been gained wide acceptance as a model system for HPV-linked oral carcinogenesis. We already have established immortalized oral keratinocytes(IHOK) transfected by E6 and E7 gene. The purpose of this study were to examined the ultrastructural features of cultured NHOK, IHOK, and HN4 oral squamous cell carcinoma cell line, and to apply these results to oral carcinogenesis in the future. NHOK from healthy retromolar pad was primarily cultured at 37oC and 5% CO2. IHOK, and HN 4 cell line which were cultured under 0.15 and 1.2mM Ca++ of KBM bullet kit. For transmission electronmicroscopy(TEM), under preconfluency, and after 3 days of postconfluency under 1.2mM Ca++, cultured NHOK, IHOK, and HN4 cell line were immediately fixed in 2.0% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4OC for 1h. The ultrathin sections were stained with uranyl acetate and lead citrate, and examined by TEM. The obtained results were as follows. 1. TEM of cultured NHOK under 1.2mM Ca++ showed increased tonofilaments, and vaculated ovoid cells with cornified envelope, while cultured IHOK showed prominent microvilli, unilateral desmosome in microvillus, and tonofilaments. 2. TEM of HN 4 cell line sowed numerous microvilli, increased N/C ratio, and lateral desmosome in microvilli under 0.15mm, while under 1.2mM well forming desmosomes. From the aboving results, under high calcium cultured IHOK showed less tonofilaments than that of cultured NHOK, while cultured IHOK, and HN 4 cell lines showed more increased desmosomes under high calcium. It was suggested that the ultrastructural changes of cultured IHOK would be accepted as intermediate stage cells for studying oral carcinogenesis.

본 실험은 자동차 시뮬레이터의 속도 변화(40, 70, 100km/h)와 롤러코스터 시뮬레이터의 운동성 구현 방법(washout 필터 적용, washout 필터 비적용)에 따른 인간 감성을 탑승자의 심리ㆍ생리적 반응을 통하여 비교 평가하였다. 자동차 시뮬레이터, 롤러코스터 시뮬레이터에 각각 건강한 20대 남자 12명, 8명을 대상으로 simulator sickness, 쾌적감, 긴장감, 각성감, 속도감에 대한 주관적 평가 및 생리신호로 뇌파의 변동리듬, 심전도, 피부전기저항을 주행 전후에서 측정하였다. 결과, 자동차 시뮬레이터에서의 저속 주행보다 운동 형태가 다양한 롤러코스터 시뮬레이터 주행시 쾌적감, 긴장감, 각성감, 속도감, 뇌파 변동리듬을 통한 쾌적도가 높았다. 뇌파의 알파파 대역의 평균 주파수는 롤러코스터 주행보다 자동차 주행에서 더 높았고, 심박률과 피부전기저항은 각 주행조건에서 주행 전후간 유의하게 변화하였다. 이상에서 주행 속도의 증가, 다양한 운동 형태의 시뮬레이터 주행시 주관적 감성은 높았고, 생리적 반응은 주행 속도 증가에 따라 활성화됨을 알 수 있었다.

Accident analyses are used to identify common factors contributing to occupational accidents and to give recommendations for accident prevention. In this study we developed a human error analysis system that can be used easily at the industries. This accident analysis system can be used to develop accident prevention programs to reduce human initiated accidents.

본 연구는 사람의 시험관아기 프로그램에서 수정 후 1일째의 전핵 등급이 체외 수정란의 발달에 미치는 영향을 조사하였다. 정상적인 시험관 아기 시술을 시행한 실례 환자를 대상으로 과배란을 유기하여 배란 직전의 난자를 채취하여 정자를 주입한 다음 체외 수정을 유도하였다. 수정 유도 18시간 후 전핵과 핵인의 형태에 따라 전핵의 등급을 1 및 2등급으로 나누어 각각 3일 동안 체외 배양을 실시하여, 체외 수정란의 형태에 따라 1, 2 및 3등급으로 분류하였든

본 연구는 사람의 시험관아기 프로그램에서 체외 수정란의 질을 향상시키는 한 방법으로 난포액으로 처리된 정자를 체외 수정에 사용하여 생산된 체외 수정란의 전핵 등급과 발달 능력을 조사하였다. 정상적인 시험관 아기 시술을 시행한 실례 환자를 대상으로 과배란을 유기하여 배란 직전의 난자를 채취하여, 난포액으로 처리된 정자와 체외 수정시킨 후 사람 체외 수정란의 전핵 등급과 체외발달율을 조사하였다. 체외수정을 위한 정자의 처리 방법으로 synthetic ser

Established SGT cell line from human submandibular gland adenocarcinoma was used to study the TGase expression on a cellular level in vitro. Transglutaminase 2(TGase 2) is assoacitated with apoptosis, GTP binding protein, and cell marix interaction. The role of TGase 2 in salivary gland tumors is not clear yet. The pupose of this study were to examine the TGase expression of SGT cell line compared to other tumor cell lines, and to apply these results to the pathogenesis of salivary gland tumor. TGase enzyme assay of SGT, SCC-15, HN 4 and HeLa tumor cell line was 3 times repeated, and calculated. Immunoslot blot for semiquantitative protein analysis was done. The obtained results were as follows. 1. SGT cell line showed the highest TGase 2 enzyme activity(about 6-16 folds) irrespective of pre or postconfluency. 2. HN 4 cell line showed the highest TGase 1 enzyme activity(about 2-3 folds) irrespective of pre or postconfluency. 3. Under postconfluency TGase 1 induction was not induced, but slightly increased in all tumor cell lines. 4. TGase enzyme activity in all tumor cell lines was accompanied with TGase protein formation. From the aboving results, the higher TGase 2 expression of SGT cell line suggested that they would come from submandibular ductal cells and have a important role in the pathogensis of salivary gland tumors.