The purpose of this study was to determine the effect of varying levels of photobiostimulation treatment dosage on plasma -endorphin concentration in humans. The subjects of this study were 21 healthy men and women, who were students of the Department of Physical Therapy, College of Health Science, Seonam University. This study was performed from October 26, 1998 to November 5, 1998. All subjects were assigned to one of three groups: a 2.0 laser group, a 4.0 laser group, an 6.0 laser group. He-Ne laser (632.8 nm wave length) and infrared laser (820 nm wave length) of three different energy densities (2.0, 4.0, and 6.0 ) were applied on the Su-Sam-Ri (L I 10) and Hab-Gog (L I 4) of acupuncture points. Blood samples were taken at pre-treatment, 30 min's post-treatment and 60 min's post-treatment. The level of endorphin was measured by radio immuno assay. The data were analyzed by descriptive statistics and repeated measure two-way ANOVA. The results of this study were as follows: 1) The human plasma -endorphin concentrations were noted to significantly increase due to the energy densities of laser photobiostimulation (p<0.05). 2) The human plasma -endorphin concentrations were noted to significantly increase during the period after laser photobiostimulation (p<0.05).

This study was undertaken to evaluate antioxidative activities of substances isolated from marine resources against human low density lipoprotein (LDL). Methanol-water extract (80:20, v/v) of Sargassum ringgoldianum had the highest antioxidant activity and the active substance was purified by silica gel column chromatography by eluting chlorform : methanol mixture (80:20, v/v). The active fraction was separated to several spots on the TLC in chloroform : methanol (10:1, v/v) mixture. Antioxidative activity of band 4 of fraction 2 on TLC was highest than that of a-tocopherol against human LDL oxidation by the method of thiobarbituric acid reactive substance (TBARS). The band 4 of fraction 2 inhibited the copper mediated oxidation of human LDL with almost completely at 1 or 2 mg/ml.

컴퓨터 기술이 급속도로 발전함에도 불구하고 사용자의 감성에 반응하는 컴퓨터는 아직 등장하지 않고 있다. 그 결과 사용자는 컴퓨터 작업 과정에서 발생할 수 있는 부정적 감성에 무방비로 노출되어 있으며, 이는 컴퓨터 작업의 효과적 수행을 저해하게 될 것이다. 본 논문은 사용자의 감성이 컴퓨터 작업에 미치는 영향을 고찰하였으며, 이를 통해 감성 컴퓨터의 개념적 구조를 제안하였다. 본 연구에서 제안한 감성 컴퓨터란 사용자 감성의 변화를 포착하고, 부정적 변화에 대응하도록 설계된다. 또한 본 연구에서는 감성 컴퓨터의 구축에 필요한 세 가지 요소, 즉 감성의 측정 및 평가, 신호 처리, 사용자 인터페이스를 중심으로 향후 연구 과제를 도출하였다. 아직 감성 컴퓨터에 대한 연구가 초기 단계인 점을 감안하면, 이에 관한 체계적인 연구가 시급한 실정이다.

사람 성장호르몬 수용체(hGHR) cDNA는 PCR방법에 의하여 fagment로서 보고되어진 바 있으나, liver cDNA로 부터 전장을 cloning한 보고는 없는 실정으로 본 연구에서는 기능을 가진 약 4.6kbp의 cDNA hGHR을 cloning 하는데 성공하였다. 먼저 cloning하기 위하여 human liver mRNA와 human breast cancer tissue로부터 회수한 mRNA를 RT-PCR방법에 의하여 human cDNA l

The pronuclear injection of metallothionein-human growth hormone (MT-hGH) gene into rabbit zygotes was performed to establish in vitro developmental system and to detect the presence of the injected gene by nested PCR. Mature female New Zealand White rabbits were superovulated by eGG and hCG treatments. The rabbits were mated and the zygotes were collected from the oviducts 18-22 h after hCG injection by flushing with D-PBS. Two to three picoliters of MT-hGH gene was microinjected into male pronuclei. The foreign gene-injected zygotes were cultured in TCM-199 or RD mediurn containing 10% FCS with a monolayer of rabbit oviductal epithelial cefls in a 5% incubator. The presence of injected DNA in rabbit embryos or blastomeres at different developmental stages .vas detected by a nested PCR analysis. The results are summarized as follows ; 1.The developmental rate of the MT-hGH gene-injected zygotes to blastocyst was significantly higher in TCM-199 medium (68.1%) than in RD medium (42.9%). 2.The gene injection into pronuclei at 18 or 22 hours post hCG treatment during pronuclear stage did not much affect on the in vitro development of the rabbit embryos. 3.The rate of gene-positive embryos detected by the nested PCR analysis was significantly decreased when they developed to blastocysts. The results indicate that the screening of transgene in rabbit embryos by nested PCR analysis could be a prornisible method for the preselection of transgenic embryos. Furthermore, the preselection of transgenic embryos would greatly reduce hoth the cost and effort of production of transgenic animals.

스피커의 음질을 평가하기 위해서 가장 많이 측정하는 것은 주파수 응답이다. 그러나 주파수 응답으로부터 바로 스피커의 성능을 평가하기는 어려운데, 이는 인간의 청각특성이 주파수나 음압에 대해 선형적인 관계를 가지지 않기 때문이다. 본 논문에서는 인간의 청각특성 중 24개의 임계대역과 라우드니스 등을 이용하여 스피커의 성능을 평가하는 방법을 제안한다. 본 논문에서 제안하는 방법은 지향서을 고려하여 스피커의 주파수 응답을 구한 후 이를 이상적인 스피커의 주파수 응답과 임계대역-라우드니스 영역에서 비교하여 음질평가지수를 산출한다. 제안된 방법에 의한 스피커의 성능 평가법은 신뢰도가 가장 큰 문제가 되므로 청취평가를 수행하여 그 결과와 비교하고 상관관계를 보였다. 제안된 방법에 의한 결과와 청취평가의 결과는 0.9765의 높은 상관관계를 가졌는데, 이는 본 논문에서 제안하는 방법이 청취평가 대용으로 훌륭히 사용될 수 있음을 의미한다.