This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of 9,889 ± 3 μg/L to 12,909 ± 2 μg/L at 45 days and 29,256 ± 4 μg/L at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to 83,751 ± 2 μg/L after 45 days from its initial value of 161,787 ± 1 μg/L and to 79,055 ± 7 μg/L at 90 days.

본 연구진은 메틸말리올라이드가 인간 피부 세포주인 HaCaT 세포에서 NRF2를 유도하고 이를 통하여 항산화 효과를 나타내는지 알아보았다. MTT assay를 통하여 메틸말리올라이드는 10 μ M 농도에서 24 h 동안 HaCaT 세포에 처리하여도 HaCaT 세포 독성을 나타내지 않는 것을 확인하였다. 본 연구진이 구축한 HaCaT-ARE-GFP-luciferase 세포에 메틸말리올라이드를 처리하고 루시퍼라아제 활성을 측정한 결과 메틸말리올라이드는 양성 대조군인 레스베라트롤보다 ARE 결합에 따른 루시퍼라아제 활성을 더욱 강하게 증가시키는 것을 확인하였다. 또한 HaCaT 세포에 메틸말리올라이드 처리 시 NRF2에 의하여 유도되는 항산화 단백질인 HO-1과 NQO1 mRNA 및 단백질 발현이 통계적으로 유의하게 증가하였다. 마지막으로 메틸말리올라이드는 HaCaT 세포에서 TPA에 의해서 유도된 DNA 및 지질의 산화를 강력하게 억제하였다. 결론적으로 본 연구는 메틸말리올라이드가 NRF2 유도를 통하여 피부 산화적 스트레스를 억제하며 이는 메틸말리올라이드가 신규 항산화 화장품의 소재로 적합하다는 것을 시사한다.

There are more than 9,300 bridges on the highway, of which about 34%(3,150) are PSCI bridges. The safety of these bridges is directly influenced by the tensile strength of the internal tendons(strand) and the strength of the concrete compared to the reinforcing bars from the design viewpoint. Therefore, in this paper, for the maintenance plan considering the damage of the internal tendons, the internal tendon cutting and truck loading test was conducted and deflection and appearance status change was analyzed. As a result of the test, deflection increased and flexural cracks occurred on center of the girder. And longitudinal direction cracks followed tendon placement occurred on a bottom flange.

In this study, the severing tests of prestressing steel in old PSCI bridges were carried out. The behavior of the bridge is investigated by measuring the displacement and strain at midpoints of severing girder. The effects of severing some prestressing steel were not noticeable enough to be visible.

Recently, there has been frequent shortage of expansion joint in expressway bridges. When that happens, maintenance budgets will be overloaded and not effective, so it is not easy to come up with a solution. In this study, we introduce a bridge inspection system that combines laser image sensing technology and automatic control technology, which have been recently applied in various fields. And this system was developed to measure bridge extension joints while traveling 100km/h.

In this study, the grouting condition and the corrosion of prestressing steel in old PSCI bridges were investigated. There are many places where the construction quality of the grout is poor to prevent the corrosion of prestressing steel. Partial surface corrosion was found regardless of the construction condition of the grout.

Post-tension system was applied most PSC bridges in Korea. Also Grouting was applied to prevent corrosion of the strand. For this reason, it is difficult to inspection the condition of the strand after completion. Therefore, in this paper, the Nondestructive testings(NDTs) developed at domestic and foreign was investigated. In addition, the current level and limit of the NDTs were analyzed considering the existing bridge environment. As a result of the study, Strand condition inspection using NDTs have been analyzed to have technical and environmental limitations.

In this paper, about 300 ground anchors which was long-term loss of tensile force occur were investigated and analyzed to determine (a)the magnitude of the load loss, (b)the causes and countermeasures of long-term loss of tensile force of ground anchors

Recently, the safety in vehicle also has become a hot topic as self-driving car is developed. In passive safety systems such as airbags and seat belts, the system is being changed into an active system that actively grasps the status and behavior of the passengers including the driver to mitigate the risk. Furthermore, it is expected that it will be possible to provide customized services such as seat deformation, air conditioning operation and D.W.D (Distraction While Driving) warning suitable for the passenger by using occupant information. In this paper, we propose robust vehicle occupant detection algorithm based on RGB-Depth-Thermal camera for obtaining the passengers information. The RGB-Depth-Thermal camera sensor system was configured to be robust against various environment. Also, one of the deep learning algorithms, OpenPose, was used for occupant detection. This algorithm is advantageous not only for RGB image but also for thermal image even using existing learned model. The algorithm will be supplemented to acquire high level information such as passenger attitude detection and face recognition mentioned in the introduction and provide customized active convenience service.

This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

Recently, there has been frequent shortage of expansion joint in expressway bridges. When that happens, maintenance budgets will be overloaded and not effective, so it is not easy to come up with a solution. In this study, we introduce a bridge inspection system that combines laser image sensing technology and automatic control technology, which have been recently applied in various fields. And this system was developed to measure bridge extension joints while traveling more than 80km/h.

The bridge coping part may cause cracking due to over-loading of superstructures. And the external prestressing method is a typical reinforcement method for a crack of coping. However, in case of design and construction error, it is difficult to expect sufficient reinforcement effect and reinforcement coping part may be damaged. For this reason, in this paper, The damage case of the coping with external prestressing method was introduced and the cause of the damage of anchorage zone was analyzed. As a result of the analysis, the cause of the damage of the anchorage zone was due to the shortage of bolt anchorage length. Also, as a result of simple calculation, the anchorage length of the bolt did not satisfy the pullout force that was occurred by the moment.

Recently, there has been frequent shortage of expansion joint in expressway bridges. When that happens, maintenance budgets will be overloaded and not effective, so it is not easy to come up with a solution. In this study, we introduce a bridge inspection system that combines laser image sensing technology and automatic control technology, which have been recently applied in various fields. And this system was developed to measure bridge extension joints while traveling more than 80km/h.

The purpose of this research is to analysis actual condition of promoting complex industrialization and changes after promoting, and then to classify organic farmer using cluster analysis based on delineated organic farmer’s changes factors. This study also aims to investigate differences in socioeconomic characteristics and behavioral intentions among classified groups of organic farmer’s changes. Data were obtained by questionnaire. From the factor analysis, four factors were derived as “Economics”, “Public benefit”, “Environment and philosophical values” and “Establishing management and the regional bedrock”. From the cluster analysis, three cluster were derived as “group that has a positive awareness of promoting complex industry”, “group that has a negative awareness of promoting complex industry” and “unstable management and a regional base”. And the three classifications were significantly different in the satisfaction and behavioral intentions.

Recently, there has been frequent shortage of expansion joint in expressway bridges. When that happens, maintenance budgets will be overloaded and not effective, so it is not easy to come up with a solution. In this study, we introduce a bridge inspection system that combines laser image sensing technology and automatic control technology, which have been recently applied in various fields. And this system was developed to measure bridge extension joints while traveling more than 80km/h.

This experiment was conducted to assess changes to the chemical properties of soil and applicability in a case of rice cultivation with organic fertilizers. The investigator applied organic fertilizers to rice cultivation for five years to examine changes to the chemical properties of soil and found that the experiment group of organic fertilizers made an ongoing increase in pH, organism content, and available phosphate content annually compared with the control group with no big differences according to the amounts of organic fertilizers used. As for the yield components, there were no statistical differences in the number of spikelets and grain filling rate between the experiment group of organic fertilizers and the control group. The experiment group recorded a higher level in 1,000 seeds weight and yield than the control group. Experiment Group 4 recorded the highest level at 29.11 kg of 1,000 seeds weight. Experiment Groups 3 and 4, which used 222 kg and 267 kg per 10 a, respectively, recorded 576 kg and 572 kg of yield, respectively, which were 4.7% and 4.1% higher than 549 kg of control group, respectively. As for the quality of brown rice, there were no statistical differences in the head rice percentage between the control and experiment group, both of which were in the range of 83.2-85.7%. As for the protein content, Experiment Groups 3 and 4, both of which used a lot of organic fertilizers, were in the range of 6.9-7.1%, which was lower than 7.5% of control group. Those findings indicate that the long-term application of organic fertilizers can improve the chemical properties of soil and increase the yield more than the conventional method of fertilizer application. The findings also suggest that it will be effective to apply 222 kg of organic fertilizers or more per 10 a.

The maintenance costs will be increased due to the aging of the bridge. Most of maintenance activity is generally known for preventing and repairing the deterioration by de-icing salts. The objective of this research is to investigate the minimizing strategy of maintenance costs for the deterioration by de-icing slats of highway bridges.

Maintenance costs, depending on the occurrence of deterioration expressway bridges has been increasing at a very rapid rate (approximately 200% increase in the last five years). In order to reduce the damage caused by water leakage and de-icing salt damage, We proposed a design improvement of concrete slab edge.

최근 이슈가 되고 있는 도심지 지반함몰로 인하여 주기적인 하수관로 조사의 필요성이 강조되고 있다. 일반적으로 수행되는 조사 방법 중 하나인 하수관로 CCTV조사는 상당한 시간과 노력이 소요된다. 기존 연구들은 주로 하수관로 조사에 소요되는 노력을 줄이기 위한 H/W 및 S/W의 개발에 관한 연구가 주를 이루고 있다. 그러나 기존 CCTV 탐사장치를 이용하여 관리담당자가 보관하고 있는 수많은 조사영상 를 활용하기 위한 연구는 진행되지 않았다. 본 연구는 cross-correlation기법 기반의 이미지프로세싱 방법을 적용하여 CCTV 조사영상의 자막 으로부터 장치의 위치정보를 추출하였다. CCTV 장치의 시간-거리 관계를 분석한 결과 탐사 장치가 정지시간과 하수관로의 손상 사이의 강한 상관관계를 확인하였다. 제안된 CCTV영상의 분석법을 활용하는 경우 CCTV조사 보고서 작성 및 관리에 소요되는 노력을 줄임으로써 하수관 로 유지관리의 효율성과 신뢰도를 높일 수 있을 것으로 기대된다.

Maintenance costs, depending on the occurrence of deterioration expressway bridges has been increasing at a very rapid rate (approximately 200% increase in the last five years). In order to reduce the damage caused by water leakage and de-icing salt damage, We proposed a design improvement of concrete slab edge.