The purpose of this study is to develop the EEG (Euchromatin Enriched Genomic) DNA library of wheat, barley, rye and oat. Mcr A and Mcr BC system in DH5 alpha bacteria cell line and Kuemkangmil, Olbori, Olhomil and Olgwiri were used for materials in our experiments. EEG colonies have been constructed by using junk DNA exclusion. We analyzed the genetic information of the colonies using blast searches of NCBI and GRAMENE web sites. One hundred eighty-four, 65, 79 and 119 STS primer pairs were developed using sequencing data of selected colonies in Kuemkangmil, Olbori, Olhomil and Olgwiri respectively. Twenty-eight and forty-two percent of designed primer pair showed polymorphism using six endoucleases in Kuemkangmil, Olbori, Olhomil and Olgwiri germplasm respectively. These primers could be useful for specific allele tagging in mapping populations and germplasm and for the study of functional genomics of wheat, barley, rye and oat.

The object of this study was to use genomic information obtained from wheat-rice sequence to develop genome-specific PCR primer for Agp-L gene involved in starch biosynthesis. Intron locations in wheat were inferred through alignment of wheat cDNA sequence of Agp-L with rice genomic sequence. Exon-anchored primer which amplify across introns allowed sequencing of introns from the three genomes provided the basis for genome-specific primer design. The single nucleotide polymorphism (SNP) was identified and this SNP could be converted into cleaved amplified polymorphism sequence (CAPS).

“Shinmichal1”, a waxy red winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Alchan”/“Chal2”during 1997. “Alchan” is a soft red winter wheat with high yield and good noodle quality. “Chal2” is a waxy wheat line crossed by “Kanto107” (Wx-A1b and Wx-B1b) and “BaiHuo” (Wx-D1b). “Shinmichal1” was evaluated as “Suwon306” in Advanced Yield Trial Test in 2002. It was tested in the regional yield trial test between 2003 and 2006. “Shinmichal1” is an awned, semi dwarf and soft red winter wheat, similar to “Uri” (check cultivar). The Heading and maturing date of “Shinmichal1” were similar to “Uri”. Culm and spike length of “Shinmichal1“ were 79 cm and 7.8 cm. “Shinmichal1“ had greater test weight (802 g) and lower 1,000-grain weight (31.8 g) than “Uri” (782 g and 35.2 g, respectively). It has resistance to winter hardiness, wet-soil tolerance, lodging tolerance and moderate post-harvest sprouting damage. “Shinmichal1” had higher flour yield (66.2%), ash (0.73%), protein (10.9%) and SDS sedimentation volume (40.7 ml) than “Uri” (64.7%, 0.53%, 9.3%, and 32.5 ml, respectively) and similar flour color to “Uri”. “Shinmichal1” had higher dough extensibility and lower dough strength than “Uri”. It is 5.2% of amylose content and had typical waxy starch pasting properties. Average yield of “Shnmichal1” in the regional adaptation yield trial was 5.24 MT ha-1 in upland and 5.58 MT ha-1 in paddy field, which was 3% and 8% lower than those of “Uri” (5.39 MT ha-1 and 6.09 MT ha-1, respectively). “Shinmichal1” would be suitable for the area above the daily minimum temperature of -10℃ in January in Korean peninsula.

“Hwanggeumchal”, a new covered waxy cultivar derived from the cross between “Chalssalbori” and “Milyang 65” with waxy endosperm and early maturing cultivars, respectively was developed at Honam Agricultural Research Institute (HARI), NICS, RDA, 2006. The origin of “Hwanggeumchal” is “Suwon 403” (SB942090-B-B-B-42-1). The initial cross was made in 1994 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 403” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hwanggeumchal”. The characteristics of “Hwanggeumchal” were the following: rate I growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 30 in upland and April 28 in paddy field, which was 1 and 4 days later than that with check cultivar, “Seodunchalbori”. The culm length was 81 cm which was 3 cm shorter than those with check cultivar. It showed spike length of 4.5 cm and 633 spikes per m2, 64 grains per spike, 27.6 g of 1,000-grain weight and 632 g of test weight. It showed stronger winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It had yellow aleurone and showed higher water absorption, expansion rate and total phenol content than those of check cultivar, but lower protein, β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 4.04 ton ha-1 in upland and 4.12 ton ha-1 in paddy field, which was 10% higher and 4% lower than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.

“Hyemi”, a new covered cultivar derived from the crosses between “Suwon 298” and “Milyang 34/Albori//Dongbori” developed at the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. The origin of “Hyemi” is “Suwon 400” (SB951032-B-B-B-28). The initial cross was made in 1995 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 400” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hyemi”. The characteristics of “Hyemi” were the following: rate Ⅳ growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 29 in upland and April 23 in paddy field, which was similar and 2 days earlier than that with check cultivar, “Olbori”. The culm length was 81 cm which was 4 cm shorter than those with check cultivar. It showed spike length of 4.1 cm and 646 spikes per m2, 52 grains per spike, 33.4 g of 1,000-grain weight and 672 g of test weight. It showed similar winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It showed higher protein content, whiteness and total phenol content than those of the check cultivar, but lower β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 3.95 ton ha-1 in upland and 4.38 ton ha-1 in paddy field, which was 13% and 16% higher than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.