Human embryonic stem cells (hESCs) have the potential for use in regenerative medicine and in the field of basic research. Therefore, effective cryopreservation and storage of hESCs are important for preservation of newly established cell line for various purposes. Despite poor survival and slow recovery after thawing, the conventional slow freezing method is most commonly used for cryopreservation of hESCs due to its simplicity and ease of use for freezing a large number of hESCs appropriate to clinical applications. Here we controlled the clump size (Group Ⅰ; 400～450 ㎛, Group Ⅱ; 800～900 ㎛, and Group Ⅲ; 1500～1700 ㎛) of hESCs at 5 days after plating using a glass pipette during cryopreservation in order to obtain a larger amount of hESCs after thawing. Attachment rates differed significantly (P<0.05) in each of the three groups and the average of attachment rate of GroupⅡ was highest in SNUhES4 and H1. In particular, the attachment rate of Group Ⅱ in SNUhES3 showed a significant improvement with ROCK inhibitor Y-27632. These results indicate that clump size and cell-cell adhesions of GroupⅡ are appropriate for cryopreservation compared to the Group Ⅰ and Group Ⅲ. This method increased cell viability and reduced the recovery time leading to various experiments, and therefore has an advantage for use with hESCs like newly established in particular. We demonstrated that use of this effective cryopreservation method with control of the clump size of hESCs can effectively improve the attachment rate and survival of post-thaw hESCs with and without Y-27632.

본 연구는 꽃장식에 있어서 침봉이나 플로랄 폼만큼 고정능력이 뛰어남과 동시에 환경에 무해하면서 미적 가치가 뛰어난 꽃 고정재료를 제시함으로써 친환경적인 꽃꽂이 방법을 제시하고자 하였다. 연구방법은 2008년 8월 독일 GBF(Grunbergr Bildungszentrum Floristik)의 플로리스트 연수과정에서 제시된 작품을 분석하였다. 고정재료는 자연에서 얻을 수 있는 재료와 재활용으로 사용되어진 재료로 나누어 분석하였다. 식물의 기관, 즉 잎, 줄기, 뿌리, 열매를 꽃 고정재료로 이용하여 장식해 본 결과 플로랄 폼 이상으로 고정 능력이 뛰어났으며 세우는 기능까지도 탁월하다는 것을 알 수 있었다. 그리고 재활용으로사용되어지는 재료들도 자연물만큼 고정능력이 뛰어나지는 않지만 꽃 고정재료가 가지고 있는 빈공간과 지탱 할 수 있는 힘을 잘 파악한 뒤에 적합한 꽃을 선택한다면 고정기능과 세우는 기능이 가능한 것을 볼 수 있었다. 이와 같이 친환경적 꽃 고정재료로써 사용되어진 식물을 포함한 자연물과 생활 속 재활용품은 침봉이나 플로랄 폼과 비슷한 고정능력과 세우는 능력을 가지고 있었다. 따라서 가급적 플로랄 폼의 사용을 자제하고 환경에 피해를 주지 않는 친환경적 꽃장식을 하는 것이 바람직할 것이다.

This study carried out development of a natural antimicrobial agent from Schima wallichii ssp. liukiuensis. Compound I exhibiting potent antimicrobial activity against Candida spp. was isolated from the methanol extracts of Schima wallichii ssp. liukiuensis. The structure of I identified as a sterol glycoside consisted of a trisaccharide and α1-sitosterol. Trisaccharide composed of L-rhamnose, D-galactose and D-glucose residues. The antimicrobial activity of I was selective on yeast rather than bacteria or other fungi. Compound I was demonstrated to be ineffective against toxicity to mouse liver cells where as protective to human dermal fibroblast cells at low concentrations. Thus, it is reasonable to expect a sterol glycoside (I) as a valuable alternative for synthetic antifungal.

벼의 질소시비량 및 수확시기에 변화에 따른 도정특성 및 쌀 품질변화를 구명하기 위하여 일품벼와 추청벼를 시험품종으로 질소시비량과 수확시기를 다르게 하여서 시험하였다. 질소시비량은 무처리를 대조구로 하여 5 kg, 9 kg, 13 kg, 17 kg, 21 kg/10a의 5수준으로 하였고 질소시비방법은 기비-분얼비-수비를 5:2:3으로 분시하였으며, 인산과 칼리의 시비량 및 시비방법은 표준재배법에 준하여 실시하였다.수확시기는 출수 후40일, 50일, 60일, 70일, 80일로 하였다. 두 품종 모두 질소시비량과 수확시기가 달라짐에 따라 도정률에 큰 변화가 없었다. 그렇지만 질소시비량은 정현비율과 정의 상관관계를 나타내었지만 현백비율과는 부의 상관관계를 나타내었다. 품종간에는 추청벼가 일품벼보다 평균적으로 모든 시비 수준에서 높은 도정률을 나타내었다. 질소시비량과 완전미율은 부의 상관관계를 나타내었다. 품종간에는 13 kg/10a 이하의 시비수준에서는 추청벼가 일품벼보다 높은 완전미율을 나타내었지만 그보다 높은 수준에서는 일품벼가 추청벼보다 약간 높았다. 두 품종 모두 질소시비량이 증가할수록 분상질미와 싸라기 발생은 높아지는 경향이었다. 질소시비량과 수확시기에 따른 쌀의 단백질함량 변화는 질소시비량이 증가할수록 단백질함량 또한 증가하였으나 수확시기에 따라서는 차이가 없었다.

Magnaporthe oryzae is a causal pathogen of the rice blast, the most destructive disease of rice worldwide. This ascomycete fungus has been intensively studied as a model organism in plant-pathogen interactions. Recently, the genome sequence of M. oryzae 70-15 was published by International Rice Blast Consortium. This led us to investigate comparative and functional analysis of this pathogen at genome-wide level. As a first step, sequencing project of M. oryzae Korean strain KJ201 was launched to face practical interests on diversity of plant pathogens. We constructed a fosmid library with a copy number controllable pCC1 vector. Over 13,000 end reads from 6637 fosmid clones were generated and anchored on genome sequence of the strain 70-15. Currently, 9,793 end reads, in which 2.684, clones with both ends and 1,757 clones with on end matched, are aligned to the sequence of strain 70-15. In the next step, shotgun sequencing of fosmid clones was conducted for fine scale comparative analysis of chromosome 7 that is well defined in genome sequencing of 70-15. Totally 119 fosmid clones were pooled in region and analyzed. For reasonable intra-species comparison in genome of M. oryzae, two strains, KJ201 and Guy11, were chosen for whole genome shotgun sequencing analysis with GS454 FLX system. About 8X genome coverage sequences were obtained for each strain and being merged for comparative analysis. Genome information of other strains is being also available from China and Japanese research groups and could be joined together for extended comparative analysis and the results give more detail in genome diversity in species level.

An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.

‘Gwangan’ is a new six-rowed covered barley cultivar developed by the National Institute of Crop Science (NICS), R.D.A in 2004. This cultivar is developed from a cross between “Mirak” and ‘Milyang59’ in 1995. An F6 selection was made at Milyang in 2001 an

“Haryeong” was bred through the potato breeding program at the National Institute of Highland Agriculture (NIHA). Itin1996. It has a round, smooth, light yelow-skined tuber characteristics and high dry mater content. It showed high resistance tolate bligh

In order to select suitable fertilizer level and sowing rate of Dangyo #8(hybrid) rapeseed at the southern area of Korea, two varieties of Naehanyuchae (Normal) and Dangyo #8(hybrid) rapeseed currently grown for oil production by two fertilizer level with 10-8-8 kg/10a and 15-8-8 kg/10a and three sowing rate with 500 g/10a, 1,000g/10a and 1,500g/10a with relatively high yield was grown at the same condition yield and yield components were observed. Dangyo #8(hybrid) rapeseed was superior to oil-eed rapeseed for yield at the 15-8-8 kg/10a of fertilizer and 1,500 g/10a of sowing rate. Fertilizer level, N-P2O5-K2O = 15-8-8 kg/10a and sowing rate, 1,500 g/10a showed somewhat higher value in two varieties of Naehanyuchae(Normal) and Dangyo #8(hybrid).

Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.