This study investigated the fumigant activity of phosphine (PH3) on 2 kinds of mealybug (Pseudococcus longispinus and P. orchidicola (Hemiptera: Pseudococcidae)) adults and nymphs. All of the two mealybugs adults showed higher LCT99 values than nymphs, and P. longispinus showed higher tolerance than P. orchidicola in a 12 L desiccator. The absorption of phosphine on 13 nursery plants showed 12.2~41.5% difference depending on the plant. All of the mealybugs treated with phosphine 2 mg/L in 0.5 m3 fumigation chamber for 4 h showed 100% fumigant activity, except P. longispinus adult (approximately 90% at bottom part). However, when the exposure time was increased to 24 h, all of them showed 100% mortality. In the treatment of 10 m3 container, the 24 h treatment of phosphine showed 100% mortality to P. longispinus and P. orchidicola adults and nymphs. In all the experiments, no phytotoxicity of phosphine observed on 13 plants until 1 month after treatment.

최근 화훼작물로 각광받고 있는 절화용 작약은 전남이 주산지로 재배면적은 7ha이며, 전국의 70%를 차지하고 있다. 주요 재배지역은 강진, 장흥이며, 비가림재배에서 발생하는 주요해충의 발생양상을 2년간 조사하였다. 잎말이나방류는 5월 하순, 7월 하순, 9월 상순에 발생하였고, 시설재배에서 심각한 피해를 주고 있다. 잎말이나방에 대한 방제약제 5종을 선발하였는데, 사이안트라닐리프롤 유제 1,000배, 에마멕틴벤조에이트 유제 2,000배, 비펜트린 액상수화제, 에토펜프록스 유제 1,000배, 클로란트라 릴리프롤 입상수화제 1,000배 처리에서 90% 이상의 방제효과를 보였다. 목화진딧물은 3월 하순부터 발생하기 시작하였고, 화뢰가 형성되기 시작하는 4월 중하순이 방제적기였다. 대만총채벌레는 3월 하순부터 발생하기 시작하였으며, 5월 하순과 7월 상순이 발생최성기였다. 뿌리혹선충은 토경재배에서 지속적으로 발생하였지만 큰 피해는 없었다.

논습지(Rice fields)는 자연습지와는 다른 복잡성과 다기능을 가지고 있으며, 수문학적 관리가 논습지 생태계의 환경에 중요한 역할을 한다. 수서무척추동물은 논습지에 서식하는 대표적인 야생동물로서 논습지 생태계에서도 먹이사슬의 모든 단계에 중요하게 위치한다. 한국의 논습지에 서식하는 수서무척추동물에 대한 연구는 일부 분류군이나 제한된 지역에서의 군집특성에 관한 연구가 주를 이룬다. 따라서 본 연구는 한반도 논습지에 서식하는 수서무척추동물 군집 전체에 관한 통합적인 연구를 위해 수행하였다. 전국 290개 지점에서 관찰된 수서무척추동물은 총 21목 60과 114종 710,892개체였고 딱정벌레목이 가장 많았고 파리목, 잠자리목, 노린재목 등의 순이었다. 논습지에 서식하는 수서무척추동물은 경지정리가 된 논에 서식하는 군집과 경지정리가 되지 않은 논에 서식하는 군집으로 나눌 수 있었으며, 경지정리가 되지 않은 논에 서식하는 군집은 다시 중부지역(경기, 강원, 충북), 서부지역(충남, 전북, 전남), 동부지역(경북, 경남)에 서식하는 군집으로 나눌 수 있었다. 이를 통해 한반도 논습지에 서식하는 수서무척추동물 군집은 경지정리의 유무와 지역적인 위치가 중요한 것을 확인할 수 있었다.

The genus Nipponopsyche Yazaki is reported from Korea with an unrecorded species, N. fuscescens Yazaki for the first time. Adult, larva, pupa and genitalia of the species are redescribed with SEM illustrations, and DNA barcode for precise identification of the species is also provided.

흑백알락나비 (Hestina persimilis (Westwood, 1850))는 한반도의 평안남도 이남의 내륙지역에 넓게 분포하고, 국외의 경우 일본, 중국, 히말라야 일대에 서식하는 것으로 알려져 있다. 본 종에 대한 생활사 연구는 김과 서(2011)에 의해 월동유충, 종령, 번데기 등에 대한 일부 보고가 있을 뿐 자세한 생활사에 대한 기록은 전무하다. 본 연구를 통해 한국산 흑백알락나비의 생태적 습성 및 미성숙단계 (알, 유충, 번데기)의 형태적 특징 등 자세한 생활사 정보를 보고하고자 한다.

One species of the genus Aphidius (Hymenoptera: Braconidae: Aphidinae) is recognized in this study. The genus Aphidius is a large braconid group distributed in the European, Palaearctic and Oriental regions, which includes currently 61 species in South Korea. This genus mainly parasitizes aphids. In this study, Aphidius transcaspicus Telenga, 1958 is recognized for the first time in South Korea. Historically, Aphidius transcaspicus is complexed with Aphidius collemani species complex. Descriptions, diagnosis distribution and illustration are provided.

The continuous emergence of chemical-mediated residual toxicity and insect resistance have enforced the regulation of synthetic pesticides. Synthetic pesticides with novel mode of actions could be developed to overcome these issues, but as an alternative biopesticides with more efficacious control activity could be developed by the advanced technology. In pest management, entomopathogenic fungi have high potential in reducing pest population to an economic threshold, and some of isolates have been commercialized. However still a novel application strategy needs to be considered for successful industrialization. An insight on fungal genes in whole genome and transcriptome levels is necessary to understand the role of genes in pathogenesis and genetic diversity of fungal genes. Herein, we have identified the genetic differences of entomopathogenic fungi using whole genome sequencing of Beauveria bassiana (Bb) and tried to understand the interaction between fungus and insect using RNA-seq. We have obtained the whole genome sequence of Bb JEF-007 using PacBio sequencing technology and compared the transcriptomes of Bb JEF-007 and bean bug, Riptortus pedestris before and after the fungal infection using Hiseq 2000 system.

Entomopathogenic fungi have been known as promising candidates for biological control of insect pests. Recently, researchers consider the fungal thermotolerance in formulations and field applications. In this study, we investigated the production of thermotolerant Isaria javanica and I.fumosorosea conidia through grain-based solid cultures and exposure to light stress. As results, of the ten grain substrates, Italian millet, rice, perilla seed and sesame, rice, sorghum produced highly thermotolerant conidia in the strains. The two strains were exposed to a light stress and showed enhanced thermal stability compared to control, when exposed to 45°C for 2 hours. This work suggests that heatresistant entomopathogenic fungal conidia can be produced by grainbased solid cultures and exposure to light stress.

세계화로 인해 교역 및 여행객의 증가로 외래 병해충의 유입이 증가하고 있고 이들의 유입 및 확산 경로 규명을 위하여 분자 유전학적 분석 방법이 이용되고 있는 실정이다. 본 연구는 외래 해충인 Reticulitermes kanmonensis의 유전적 특성을 분석하기 위한 18개의 초위성체마커(microsatellite)를 이용하여 총 16개 지역(전주, 김제1, 김제2, 군산1, 군산2, 서천, 완주1, 완주2, 완주3, 완주4, 익산1, 익산2, 논산, 일본1, 일본2, 일본3)의 흰개미 244개체를 분석하였다. 집단간 대립 유전자 패턴(allelic patterns)분석결과 일본3 지역의 유전적 다양성이 높게 나타났고 완주2 지역은 다른 지역에 비해 비교적 낮게 나타났다. 집단 간 유전적 거리(Genetic differentiation)는 평균적으로 0.163정도의 유전적 다향성 비율을 나타냈고, 서천과 군산2 지역이 0.048로 낮게, 서천과 일본2 지역이 0.442로 높게 나타나 서천과 군산2 집단이 유전적으로 가깝게 나타나고, 반면 서천과 일본2 집단과는 유전적으로 멀게 나타났다. STRUTURE 분석결과는 클러스터 집단 수가 6(k=6)일때 이상적인 집단 패턴을 나타내었고, 유전적 구조는 A그룹(전주, 김제1, 완주2), B그룹(서천, 군산1, 군산2), C그룹(완주1, 김제2), D그룹(논산, 익산1, 익산2), E그룹(김제1, 완주2, 완주3), F그룹(일본1, 일본2, 일본3)의 6가지의 패턴이 지역별로 분리되어 나타났다.

A combination using phosphine (PH3) and ethyl formate (EF) was performed to compensate for the disadventages (long exposure time and phytotoxicity) of a single substance. P. longisipinus was more susceptible to mixed phosphine and ethyl formate than P. orchidicola in 12 L dessicator. Mortality of mixed treatment was higher than aggregated mortalities that treated individually, so it indicated to have a synergic effect on each other. Ethyl formate was showed higher adsorption rate than phosphine on imported nursery plants and showed differences depending on the plant. P. longispinus and P. orchidicola was showed 100% mortality when phosphine and ethyl formate was treated as 1 g/m3 and 30 g/m3 for 4 hour in 0.5 m3 fumigation chamber and in 10 m3 container. Phytotoxicity was evaluated to mixture of phosphine and ethyl formate at 1 month after treatment.

과일이나 농작물의 부패 및 발효 환경에서는 Methanol, Ethanol, Acetic acid을 비롯한 다양한 화학물질들이 생산된다. Drosophila melanogaster는 이러한 발효·부패 환경에 서식하면서 일정 농도 이상의 다양한 화학물질에 지속적으로 노출되어 생존하도록 적응되어온 것으로 생각된다. 다양한 화학물질이 포함한 환경에 안정적으로 서식하기 위해서는 D. melanogaster는 화학물질에 능동적으로 반응하여 해독 유전자나 대사 관련 유전자의 발현량을 변화 시킴으로써 발효·부패 환경에서 생성되는 화학물질에 대한 높은 내성을 가지고 있을 것으로 판단된다. 현재까지 유전자의 발현량 측정을 위해 real-time PCR를 이용하여 reference gene의 발현량을 기준으로 정량화하는 방법이 가장 널리 사용되고 있다. 그러나 조직별, 환경별, 발달단계를 비롯한 다양한 조건에서 안정적으로 발현되는 reference 유전자 선정이 필수적으로 선행되어야 하므로 본 연구에서는 발효·부패 환경에서 생산되는 두 화학물질인 Methanol과 Ethyl Acetate에 노출된 D. melanogaster에서 안정적으로 발현되는 reference gene을 찾는 연구를 실시하였다. 본 연구에서는 다양한 농도의 Methanol과 Ethyl Acetate을 D. melanogaster에 노출시킨 후 RNA 추출과 cDNA 합성을 실시였고, 5가지 후보 reference gene (hsp22, nd, rpL18, tbp and ef-1b)의 안정적 발현 여부를 qRT-PCR을 통해 조사하였으며, 유전자 발현의 안정성을 측정하는 3가지 프로그램(geNorm, NormFinder, BestKeeper)을 이용해 비교·분석하였다. 본 학회에서는 연구의 과정과 그 결과를 발표하고자 한다.

Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, 43 alkyloxyalcohols were synthesized and nematicidal activity was tested against PWN. As a control, monochamol, which reported to have nematicidal activity was also tested. The nematicidal activity was diffferent according to total carbon chain length in compounds and carbon numbers of diol derivates. The odd numbered carbon diol derivates (ROC5OH, ROC7OH, ROC9OH) showed weak activity. The compounds which had C14H30O2 formula (C11OC3OH, C10OC4OH, C8OC6OH, C6OC8OH) showed the same level nematicital activity as that of monochamol in the term of LC50 value. Thee compounds which had C15H32O2 and C13H28O2 formulas (C12OC3OH, C11OC4OH, C12OC3OH, C10OC3OH, C9OC4OH, C5OC8OH) were followed.

Japanese pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae) is considered as a serious pest in pine trees. To develop an eco-friendly strategy to manage this forest insect, we collected entomopathogenic fungi from Korean soil and assessed their virulence against the adults of the insect in laboratory conditions. As a result, two isolates with conidial suspension (1.0×107conidia/ml), showed 87% and 90% mortality 12 days after fungal treatment, respectively. We assessed the potential of the fungi-derived destruxin and protease as additives to the fungal isolates, and they showed insecticidal activity via feeding and spraying treatments. Finally, we produced fungal conidia in massive solid cultures and formulated wettable powders, and now studying optimal conditions of oil-based formulation with two isolates. Based on these results, we are evaluating the control efficacy of the fungal agents against M. alternatus in field conditions.

Background: Despite muscle latency times and patterns were used as broad examination tools to diagnose disease and recovery, previous studies have not compared the dominant arm to the non-dominant arm in muscle latency time and muscle recruitment patterns during reaching and reach-to-grasp movements. Objects: The present study aimed to investigate dominant and non-dominant hand differences in muscle latency time and recruitment pattern during reaching and reach-to-grasp movements. In addition, by manipulating the speed of movement, we examined the effect of movement speed on neuromuscular control of both right and left hands. Methods: A total of 28 right-handed (measured by Edinburgh Handedness Inventory) healthy subjects were recruited. We recorded surface electromyography muscle latency time and muscle recruitment patterns of four upper extremity muscles (i.e., anterior deltoid, triceps brachii, flexor digitorum superficialis, and extensor digitorum) from each left and right arm. Mixed-effect linear regression was used to detect differences between hands, reaching and reach-to-grasp, and the fast and preferred speed conditions. Results: There were no significant differences in muscle latency time between dominant and non-dominant hands or reaching and reach-to-grasp tasks (p>.05). However, there was a significantly longer muscle latency time in the preferred speed condition than the fast speed condition on both reaching and reach-to-grasp tasks (p<.05). Conclusion: These findings showed similar muscle latency time and muscle activation patterns with respect to movement speeds and tasks. Our findings hope to provide normative muscle physiology data for both right and left hands, thus aiding the understanding of the abnormal movements from patients and to develop appropriate rehabilitation strategies specific to dominant and non-dominant hands.

본 연구에서는 국내 유통 중인 농산물 9품목(n = 578)에 대한 납과 카드뮴 함량을 조사하고 이들의 섭취로 인한 위해성을 평가하고자 하였다. 납과 카드뮴의 함량은 마이크로웨이브 분해 후 ICP-MS로 분석하였다. 조사대상 농산물의 납 평균 함량은 각각 보리 0.014 mg/kg, 완두콩 0.010 mg/kg, 강낭콩 0.008 mg/kg, 녹두 0.006 mg/kg, 파인애플 0.008 mg/kg, 살구 0.016 mg/kg, 매실 0.015 mg/kg, 자두 0.021 mg/kg, 대추 0.019 mg/kg이었고, 카드뮴 평균함량은 보리 0.017 mg/kg, 완두콩 0.004 mg/kg, 강낭콩 0.007 mg/kg, 녹두 0.005 mg/kg, 파인애플 0.001 mg/kg, 살구 0.002 mg/kg, 매실 0.002 mg/kg, 자두 0.002 mg/kg, 대추 0.003 mg/kg이었다. 모든 시료의 납, 카드뮴 함량은 EU, CODEX 및 국내 기준보다 낮은 수준이었다. 조사 대상 농산물에 대한 납, 카드뮴의 인체노출량을 산출한 결과, 납은 잠정주간섭취허용량(PTWI, 25 μg/kg b.w./week)의 0.067%, 카드뮴은 월간잠정섭취허용량(PTMI, 25 μg/kg b.w./month)의 0.28%이었다. 이상의 결과는 조사 대상 농산물의 납, 카드뮴 오염도와 이들의 섭취에 의한 위해성이 모두 낮은 수준이라는 것을 보여준다.

Introduction Recent years have witnessed a rapid growth in peer to peer (P2P) sharing-service businesses such as Uber and Airbnb. In P2P sharing-service businesses, goods or services are provided by customers (peers) rather than by service firms, who act simply as an intermediary between customers. One customer acts as a service-providing customer (SPC), and the other as a service-receiving customer (SRC). P2P sharing-service firms have no direct control over an SPC’s quality of service provided to an SRC. Further, both SPCs and SRCs are customers to the firm, and therefore firms are concerned with the quality of service provided not only to SRCs but also to SPCs. In the P2P sharing-service context, particularly in the case of services serving the needs of diverse travelers (e.g., Uber or Airbnb), SPCs and SRCs can be people of different genders and races, which can cause them to feel socially distant from each other. Since SPCs are not professional service employees, they may not know how to cope with the social distance, which can result in uncomfortable service experience for both SPCs and SRCs. The more similar to the SPC an SRC feels, the more comfortable the SRC is likely to feel, which can lead to higher service satisfaction. Yet, few studies examined how such social distance can be reduced. This study is intended to fill this gap in the research. Specifically, we propose that an SPC’s form of address for SRC can moderate the effect of the incongruence in gender and race on an SRC’s perceived social distance. Theoretical Development Social distance refers to the level of acceptance people have of others outside of their own social group or class (Bogardus, 1928). It is a measure of perceived difference (or distance) between groups. In the context of the P2P sharing service, SRCs and SPCs can be from diverse social groups. When an SRC encounters an SPC from a social group that is different from theirs, the SRC can feel socially distant from the SPC. Immediate differences an SRC can identify upon meeting an SPC is demographic such as gender, age and race. In this study, we first propose that the difference (incongruence) in gender, age and race makes an SRC feel socially distant from an SPC. Social distance is closely related with similarity (Osbeck & Moghaddam, 1997; Liviatan, Trope & Liberman, 2009). In the context of mentoring, the higher the perceived and actual similarity a portage feels with a mentor, the higher the level of the portage liking and satisfaction for the mentor and with the mentoring service (Ensher, 1997). Matching gender and race between a mentor and a portage positively influenced self-reported grade point average, efficacy and confidence of a portage (Blake-Beard et al. 2011). Race was well demonstrated to influence social distance (Triandis & Triandis, 1960). Taken together, we conjecture that the effect of the difference in gender and race on social distance will apply to the P2P sharing service context and propose the following hypothesis: H1: The incongruence in gender and race between an SRC and an SPC will make SRCs feel more socially distant from SPCs compared to the case of congruence. We propose in this study that the form of address for SRCs by SPCs can influence the level of social distance SRCs feel because of the incongruence in gender and race. The relationship between forms of address and social distance has been proposed (Brown, 1965). Intimate terms of address is associated with intimate relationship. Intimate terms of address is inversely proportional to social distance (Keshavarz, 2001). Calling someone by the first name is related with friendliness (Brown, 1961). The use of first name is positively associated with closeness in relationship (Brown, 1965). An empirical study in the healthcare context showed that most patients preferred to be addressed by the first name (as opposed to last name) (Gillette, Filak & Thorne, 1984). Taken together, we propose the following: H2: Intimate forms of address by the SPC for the SRC will reduce the level of social distance caused by the incongruence in gender and race. We propose that the level of perceived social distance influences the level of comfort SRCs feel with the SPC during the service delivery. The negative effect of social distance on the level of interaction comfort has been shown (Paswan & Ganesh, 2005). In the context of service encounters where customers feel cultural differences, psychological distance was shown to influence comfort negatively (Weiermair, 2000). Since social distance is a dimension of psychological distance (Trope & Liberman, 2011), we propose the effect of social distance on comfort as following: H3: Reduced social distance will lead to a higher level of comfort. The effect of social distance on comfort can vary by customers. In this study, we propose that the effect is moderated by the customer’s motive for the purchase of the P2P sharing service. There are largely four movies for customers who participate in collective consumptions (i.e., sharing service): economic motive, social motive, hedonic motive, and the motive to reduce risks and responsibilities (Benoit, Baker, Bolton, Gruner & Kandampully, 2017). Economic motives are associated with reducing expenses, and social motives are with meeting other people (e.g. more authentic travel) (Benoit et al., 2017). Hedonic motives are related with “accessing products that are exciting or normally out of reach” while motives to reduce risks and responsibilities are related with “no burdens of ownership, option to preview a product for potential purchase” (Benoit et al., 2017). According to a research in the context of P2P accommodations, cost saving, familiarity, trust, and utility are determinants of satisfaction with a sharing option. Thus, we propose that customer motives moderate the effect of social distance on comfort (Möhlmann, 2015). H4: The effect of social distance on comfort vary by customer motives for purchase. When feeling comfortable, people are more likely to trust, feel satisfied, and commit themselves, which can help improve relationship (Spake, Beatty, Brockman & Crutchfield. 2003). Comfort positively influences perceived service quality and satisfaction (Dabholkar, Shepherd & Thorpe, 2000). Comfort influences satisfaction positively (Paswan & Ganesh, 2005). In the P2P sharing-service context, SRCs’ evaluation of SPCs are carried out by reviews. Customer reviews of SPCs’ services are vitally important for both SRCs and SPCs. Reviews help other SRCs to identify desirable SPCs (Ert, Fleischer & Magen, 2016) and SPCs to receive feedback for their service quality improvement. Therefore, we propose the following: H5: Comfort leads to SRC’s intention to write good reviews. Data Collection Data will be collected from American consumers who have used Uber at least once in the past one year through an online scenario-based survey using a 2 (genders: male vs. female) x 3 (races: white vs. yellow vs. black) x 3 (forms of address: no address vs. first name vs. last name) between-subject experimental design. Hypotheses will be tested by an analysis of variance and a structural equation modeling analysis. In the analyses, the potential effects of trust, familiarity, community belonging, utility (Möhlmann, 2015) and age will be controlled. Implications Findings of this study will reveal the importance of reducing social distance that SRCs feel during encounters with SPCs. Of many possible ways to reduce social distance between SRCs and SPCs, the result of this study will show that SPCs’ use of appropriate form of address to SRCs is effective. Further, it will show that the effect of social distance on customer comfort can vary by the purpose of the use of the sharing service. These findings will offer P2P service firms insights on how to help SPCs offer more comfortable services to SRCs and as a result receive positive reviews from SRCs.

Recently, the studios in Hollywood introduces mobile games based on the storyline of movies for promotional purpose. With wide exposure of such games, managers could expect raised market awareness and purchase intention toward movies set to be released. Among the movie-themed mobile games, at the same time, not a few games have made financial success as the box office scores of the original movie increases. Thus, managers should deal with the promotional mobile app and the target product simultaneously on the belief of reciprocal relationship between them. If the dynamic relation is true then, finding deterministic factors of mobile game performance has practical significance to make positive consequences for both products. Branded entertainment is defined as “the integration of advertising into entertainment content, whereby brands are embedded into storylines of a film, television program, or other entertainment medium” (Hudson and Hudson 2006). Wise et. al. (2008) states that the advergame, a videogame designed around a brand, is one form of branded entertainment. Additionally, Sood and Drèze (2006) examined film sequels as brand extension of experiential goods based on the reality that Hollywood brands movies. In this vein, movie-themed mobile games can be contemplated as a new practice of branded entertainment. To test the research hypotheses, we collected 43 pairs of a movie and a movie-themed mobile game. Among 300 movies in the annual top 100 U.S. domestic box office chart from 2012 to the end of October 2014, films having more than one promotional mobile game were selected. The data on mobile game were limited to the Apple’s App Store for consistency. For data collection, we utilized multiple archival sources such as boxofficemojo.com, metacritic.com, imdb.com, and appannie.com. The objective of this research is to investigate the impact of movie-themed mobile games on the performance of original movies and the characteristics leading mobile game success. We developed a simultaneous equation system to discover the interdependent relationship between mobile game ranking and movie sales. This study is one of the first empirical investigation demonstrating reciprocal relationship between promotional game app and its target product. The results show that mobile games perform profit generating function as well as promotional function, thus suggesting managers to deal with mobile game more strategic way.

스탠다드국화 신품종을 육성하기 위하여 충남농업기술원 화훼연구소에서 2009년에 황색의 모본 ‘Summer Yellow’와 부본 ‘ST07-09-02’계통을 인공 교배하였다. 2010년에 종자를 파종하였고, 이중 내병성이 강하고 기호성이 우수한 개체를 선발하여 ‘ST10-047-01’로 계통명을 부여하였다. 2011년부터 2013년까지 주년 생산성을 위해 촉성 및 자연, 억제재배 특성을 각각 검정하였으며, 2013년 ‘Geumhwa’로 명명하고 품종등록 출원하였다. ‘Geumhwa’의 생육 및 개화특성은 국내에서 많이 재배되고 있는 황색 스탠다드국화인 ‘Summer Yellow’를 대조 품종으로 하여 조사하였다. ‘Geumhwa’ 품종은 자연개화기가 10월 6일로 ‘Summer Yellow’의 10월 25일에 비해 빨랐다. ‘Geumhwa’는 초장이 86.3cm로 ‘Summer Yellow’의 93.8cm보다 작았고, 곁가지 제거수는 8.1개로 ‘Summer Yellow’의 16.6개보다 적었다. ‘Geumhwa’의 꽃직경은 13.6cm로 ‘Summer Yellow’의 13.5cm와 비슷하였으며, 꽃잎수는 ‘Geumhwa’가 263.6개로 ‘Summer Yellow’의 295.3개보다 적었다. 재배상 유의사항은 ‘Geumhwa’는 중간종이므로 초기생육이 왕성하도록 비배 관리를 하고 생육기간 중 지베렐린 1,000mg･L-1를 2회 처리하여 신장력을 높이도록 한다. 또한 설상화수가 적은 편이므로 재전조를 실시하여 설상화수를 늘린다면 황색의 연중 조기개화가 가능한 고품질 신품종 스탠다드 절화국화로써 소비자 기호 충족 및 농가소득 창출에 기여할 수 있을 것으로 기대된다.

Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelial-mesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.

Mycoplasma spp. are extracellular bacteria that colonize on the respiratory epithelium of humans and animals. It is a causative agent of pneumonia commonly complicated by opportunistic infectious bacteria. Mycoplasma spp. infection cause relatively mild disease in the absence of environmental stressors, but when complicated by secondary bacterial invaders the resultant disease can cause obvious clinical disease and severe production losses in intensively reared pigs Mycoplasma spp. are highly fastidious bacteria, difficult to culture and slow growing. Many species of Mycoplasma spp. are important pathogens causing respiratory infection in animals and known to induce huge economic losses. The aims of the present study were to develop a rapid isolation and culture method of wild type Mycoplasma spp. in pigs. We used Mycoplasma spp. genus specific direct PCR without DNA extraction procedure using PhireⓇ Animal Tissue Direct PCR Kit from the lung tissues with pneumonia lesions. Therefore, we could save the time for tissue processing and increase the accuracy of Mycoplasma spp. inclusion prediction in lung tissues. Thereafter, we used the optimized media to isolate and culture Mycoplasma spp. As the results, Mycoplasma spp. could be isolated and cultured quickly and efficiently. These results could provide an efficient strategy and method for the rapid and accurate isolation and culture of wild type Mycoplasma spp. in pigs.