본 연구를 통해 국내에 분포하는 중기문응애류 중 파리응애과의 Holostaspella crenulata Krantz, 1967 (톱니무늬파리응애, 신칭) 와 화살응애 과의 Lasioseius floridensis Berlese, 1916 (가슴선화살응애, 신칭) 를 처음으로 확인하고, 각 종에 대한 분류학적 진단과 분포정보, 성충의 현미경사 진과 도판을 제시하였다.

가는잎향유[Elsholtzia angustifolia (Loes.) Kitag.]는 화형 이 아름답고, 정유 특유의 향기가 좋아서 분화용 및 지피용 관 상식물로 수요가 증가하고 있고, 전초에는 약효가 있다고 알 려져 있다. 본 연구는 가는잎향유의 육묘에 미치는 플러그 트 레이 셀 사이즈, 파종립수, 차광정도, 추비농도 등의 영향을 구명하기 위하여 수행되었다. 연구결과, 플러그 트레이 셀 사 이즈는 용량이 증가할수록 유묘의 초장, 엽수, 마디수, 근장, 지상부 생체중이 유의적으로 증가하였다. 파종 립수는 2립 파 종 시 가장 효율적이었고, 파종량이 증가할수록 생육이 감소 하였다. 차광정도가 높아질수록 초장은 증가하였고, 경직경, 엽수, 마디수는 55% 차광에서 가장 우수하였다. 추비 처리 시 공시비료 1000배 처리구에서 생육이 가장 양호하였다. 따라 서 가는잎향유의 육묘 시 162셀 트레이에 원예상토를 채운 다음 셀 당 2립 파종한 후 55% 차광막이 설치된 육묘상에서 공시비료 1000배로 엽면시비하는 것이 가장 효과적인 것으로 생각된다.

This study develops and validates an English communicative competence model for Korean high school students, in response to the need to redefine the relevant concepts and components of competence that are demanded by the rapidly evolving future society. Drawing on Celce-Murcia’s (2008) theoretical model on communicative competence, this research conceptualized a model that could assess high school students’ English communicative competence by examining relevant domestic and international studies as well as theoretical reflections. Expert opinions from a two-stage Delphi survey were compiled and incorporated to revise, supplement, and validate the English communicative competence among high school students reflecting Korea’s English education environment. Following this process, the conceptual model for English communicative competence was reorganized into five sub-competences (sociolinguistic, discourse, linguistic, interactional, and strategic competence) and 15 corresponding subfactors. The content validity ratio values for the conceptual definition and factor structure of this model were all above 0.64, thus affirming the validity of the conceptual definition and factor structure.

Background: Various treatments are being tried for lumbar neuropathy. Among them, neural mobilization aims to reduce peripheral nerve sensitivity by stimulating them directly. Similarly, applying kinesiotape stimulates skin and joint receptors around the nerves, thereby affecting nerve function and movement. Objectives: To investigated the effect of low back pain on the neural mobilization and kinesiotaping. Design: Randomized controlled trial. Methods: Thirty patients with chronic low back pain were randomly allocated to three groups: the neural mobilization exercise with kinesiotaping group (NEKTG, n=10), the neural mobilization exercise group (NEG, n=10), and the kinesiotaping group (KTG, n=10). Each group performed six intervention sessions over two weeks. The results were analyzed by measuring the numeric pain rating scale (NPRS) and straight leg raise (SLR) before and after the intervention in each group. Results: NEKTG, NEG, and KTG all indicated a significant decrease in the back pain index before and after the intervention. However, there were no differences observed between the three groups. The SLR angles before the experiment were identical across the three groups. The SLR angles before and after the intervention were as follows: the SLR angle significantly increased in NEKTG. Furthermore, there was a significant increase in the SLR angle in NEKTG compared to both NEG and KTG. Conclusion: Neural mobilization combined with kinesiotaping provided better therapeutic effects regarding pain reduction and neurodynamic improvement compared to alone kinesiotaping and mobilization with chronic low back pain.

본 연구는 한국어 학습자들이 자신들의 언어학습 신념이 그들의 전반 적인 언어학습과 새로운 환경에 적응하는 것에 어떠한 관계가 있는지를 찾기 위함이 목적이다. 여섯 명의 한국대학교에 재학 중인 외국인 유학 생들을 대상으로 그들의 언어학습 신념에 대한 인터뷰가 진행되었다. Horwitz의 BALLI 문항을 기반으로 한 반구조적 인터뷰가 진행되었으며, 그들의 답변은 외국어 능력, 언어학습의 어려움, 언어학습의 본성, 학습 과 소통전략, 동기와 기대 등의 주제를 기반으로 비슷한 패턴을 보이는 항목별로 구분되어졌다. 연구 결과는 외국인 유학생들은 그들의 한국어 실력이 향상될수록 자신감을 가졌으며, 그들은 문화에 대한 학습이 언어 학습에 중요한 부분이라는 것을 인지했다. 또한, 가장 효과적인 학습법은 실제적인 상황과 사람들과 어울리는 것을 꼽았으며, 그들은 대학 졸업 이후에도 한국에 남아 있기를 희망했다.

This study examines the rise of the Body Positive Movement on TikTok and its role as a form of online content activism influencing the fashion design and industry. Through a combination of literature review and case study methodology, the study explores the expression techniques and thematic types of Body Positive Movement on TikTok. Reviews of literature, previous studies, online articles, fashion journals, and relevant search terms on TikTok informed a definition of Body Positive Movement and an analysis of its formation and rise. The research findings confirm the impact TikTok content on Body Positive Movement has on the fashion industry in addressing external factors (i.e., ‘Appearance’, ‘Race’, ‘Aging’, ‘Physical Disability’) and intrinsic factors (i.e., ‘Acceptance of Diversity’, ‘Self-Esteem’, ‘Rejection of Stereotypes’, ‘Appropriate Representation’, ‘Information Provision’). The key external factor , ‘Appearance’, includes subcategories such as ‘Body Shape’, ‘Body Hair’, ‘Skin’, and ‘Facial Features’. TikTok content creators on fashion creatively combine music, emojis, and visual storytelling to exhibit positive self-perception concerning these factors. A significant finding of the study is that short clips predominantly manifesting external factors differentiate into informative or enlightening videos associated with intrinsic factors. The study underscores Body Positive Movement's important influence on the fashion industry from design to presentation.

The secondary growth model for Salmonella was developed based on the artificial neural network (ANN) with data collected from ComBase and FoodData Central. In addition to the existing secondary model variables (temperature, pH, Na+, and water contents), more input variables (sugar, carbohydrate, lipid, and protein contents) were considered. The output variables were microbial growth parameters (lag phase duration [l] and maximum growth rate [mmax]). A commercial ANN program (NeuralWorks Predict) was utilized with training at 80%, validation at 10%, and test data at 10%. ANN models were created using all data and cleansed data. Using the cleansed data, the training/testing root mean square error (RMSE) for mmax improved from 0.14/0.16 to 0.11/0.14, whereas the RMSE for l was still not acceptable, from 11.94/33.03 to 7.09/4.18. The l data were divided into two ranges with high and low goodness of fit, whereas the ANN model for each field was built, resulting in an optimally low RMSE.

Background: Effective trunk stabilization has been a cornerstone in physiotherapy, particularly for individuals with lower back issues. While bridging exercises were traditionally employed for this purpose, there has been a growing interest in their modified versions to optimize therapeutic benefits. Objectives: To investigated the differential effects of traditional and modified bridging exercises, particularly when varying leg support and integrating abduction maneuvers during sling-assisted exercises, on trunk muscle responsiveness. Design: Cross-Sectional study. Methods: A group of twenty participants was subjected to three exercise protocols: Bilateral Limb Bridging (BLB), Single Limb Bridging (SLB), and Single Limb Bridging combined with Hip Abduction (SLBHA). Using Surface Electromyography (EMG), the study captured the activation patterns of the Internal Oblique (IO), Erector Spinae (ES), and Multifidus (MF) muscles. Statistical analysis was conducted using the Kruskal-Wallis test, with post-hoc examination for detailed insights. For data consistency, normalization was executed based on Maximum Voluntary Isometric Contractions, and EMG data interpretation was conducted using the RMS technique. Results: The most prominent variations in muscle activation were identified in the IO muscles on both sides. The left IO displayed marked activation disparities between BLB vs. SLB and SLB vs. SLBHA. Analogous observations were made for the right IO when comparing BLB to SLBHA and BLB to SLB. Conversely, ES and MF muscle activations remained consistent across the different exercises. Conclusion: Modified bridge exercises with sling-assisted leg supports with abduction can selectively activate IO muscles, with a noticeable asymmetrical effect favoring the left side.

Despite its profound impact on athletic performance, the significance of heart rate recovery (HRR) has been insufficiently addressed in the filed of sports science, particularly in the context of weightlifting characterized by brief and intense exertions involving heavy weights. Serving as a valuable indicator of autonomic nervous system and cardiovascular function, HRR assumes a pivotal role in weightlifting. This comprehensive review aims to delineate the specific demands for HRR in weightlifting, shedding light on the often overlooked cardiovascular considerations within training regimes focused on strength and power. The investigation scrutinizes the repercussions of HRR on weightlifting performance, seeking to elucidate how inadequate recovery intervals may result in physiological and psychological consequences. These consequences encompass a distorted perception of effort, disruption of coordination, compromised posture due to irregular breathing, and an overall decline in lifting capacity. The review systematically presents compelling evidence pertaining to heart rate response and recovery patterns during weightlifting, underscoring the critical importance of well-structured rest periods. Furthermore, the review delves into a comprehensive discussion of factors influencing HRR in weightlifting, encompassing variables such as sex, age, cardiovascular function, hydration, nutrition, and psychological aspects. Finally, a key emphasis is placed on the integration of effective HRR techniques into the training regimens of weightlifters, thereby ensuring sustained and optimized performance outcomes.

Hypertension is characterized by excessive renin-angiotensin system activity, leading to blood vessel constriction. Several synthetic compounds have been developed to inhibit renin and angiotensin-converting enzyme (ACE). These drugs often have adverse side effects, driving the exploration of plant protein-derived peptides as alternative or supplementary treatments. This study assessed the phenolic compound and amino acid content and the antioxidant and antihypertensive activity of 5 South Korean staple crops. Sorghum had the highest phenolic compound content and exhibited the highest antioxidant activity. Millet grains, particularly finger millet (38.86%), showed higher antihypertensive activity than red beans (14.42%) and sorghum (17.16%). Finger millet was found to contain a large proportion of branched-chain, aromatic, and sulfur-containing amino acids, which are associated with ACE inhibition. In particular, cysteine content was positively correlated with ACE inhibition in the crops tested (r=0.696, p<0.01). This study confirmed that the amino acid composition was more correlated with the antihypertensive activity of grains than the phenolic compound content. Finger millet mainly contained amino acids, which have higher ACE inhibitory activity, resulting in the strongest antihypertensive activity. These findings underscore the antihypertensive potential of select crops as plant-based food ingredients, offering insight into their biological functions.

본 연구는 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’에 있어서 삽목배지 및 삽목시기가 발근에 미치는 영향을 조사하 기 위해 수행하였다. 삽목배지 실험에서 코코피트, 펄라이트, 피트모스 단용과 코코피트+코코칩을 혼용 처리하였으며, 7월 15일에 ‘Morning Star’의 정아에서 삽수를 채취하여 삽목하 였다. 삽목시기 실험은 4월, 6월, 8월에 정아를 채취하여 삽 목하였다. 삽목배지로써 코코피트와 코코칩의 혼용 배지는 발 근에 가장 부정적인 영향을 미쳤다. 다른 배지들은 발근율과 뿌리길이에서 통계적인 차이가 없었으나, 펄라이트와 피트모 스보다 코코피트에서 뿌리수가 더 많았다. 따라서 국내육성 수국 ‘Morning Star’의 삽목은 코코피트를 배지로 사용하는 것이 발근에 가장 효과적인 것으로 나타났다. 삽목시기에 관 한 실험에서, ‘Morning Star’의 발근율은 삽목시기와 상관없 이 83~90%로 통계적인 유의성이 없었으나, 뿌리수와 생체 중은 4월과 8월보다 6월에 삽목했을 때, 가장 증가하였다. 그 리고, 신초의 길이, 엽수 등의 줄기의 생육도 6월에 삽목한 처 리에서 가장 좋았다. ‘Pink Ari’의 삽목시기 실험에서 발근율 은 4월 삽목보다 6월과 8월 삽목 처리에서 높았고, 뿌리수와 뿌리 생체중은 6월 삽목처리에서 각각 61.4개와 0.31g으로 가장 양호하였다. 또한 엽수와 신초의 길이 등 지상부 생육도 전반적으로 4월과 8월 삽목보다 6월 삽목처리에서 가장 좋았 다. 따라서, 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’는 4 월과 8월보다 6월에 삽목하는 것이 발근과 지상부 생육에 효 과적인 것으로 나타났다.

The economy of Tanzania relies on agriculture as the main economic activity, because agriculture provides both food and income to the population of the country, especially the rural households. Improvement of market access to crops also increases the productivity. Therefore, in this study, descriptive statistics and statistical analysis of the probit model were used to analyze the factors that determine the market accessibility of small-scale farmers in Chemba, Tanzania. Cross-sectional data collected by the systematic sampling method for 200 corn peasants in Chemba, Tanzania were used. As a result of probit regression analysis, it was found that access to improved seeds and technology had a positive statistically significant effect on the accessibility to the market, while the age of the head of the household, production cost, distance to the market, and household size had a negative statistically significant effect. Therefore, in this study, policy establishment and implementation are recommended. A policy needs to be considered to reduce the transaction costs that eventually allows the farmers to increase the accessibility to markets, enables small-scale farmers to participate in cooperatives, lowers input costs and provides educational programs on quality products to increase their competitiveness in the marketplace.

이 연구는 1세대 스마트 온실의 재배환경 데이터와 장미 절 화의 품질 특성 데이터를 수집하고 그 요인들 간의 상관 관계 를 분석하여 절화수명 예측 및 최적 환경 조성의 기초 자료를 얻고자 수행되었다. 이를 위해, 토경재배(SC) 및 암면배지경 양액재배(RWH) 하우스 각 1개소를 선정하여 1년간 기온, 상 대습도(RH) 및 수증기압차(VPD), 일적산광량(DLI), 근권온도 등의 환경 데이터와 매월 말 수확된 장미 ‘Miss Holland’ 절 화의 품질 특성 데이터를 수집하였으며, 이 데이터와 절화수 명과의 상관관계를 분석하였다. 절화수명은 10월과 11월을 제외하고는 SC 하우스에서 RWH 하우스보다 더 길었다. 절 화수명과 환경 및 생육 특성 간의 상관관계 분석에서 SC 하우 스의 상관계수는 RWH 하우스보다 조금 더 높았으며, 절화수 명 예측을 위한 요소들도 두 하우스 간에 차이가 있었다. SC 하우스의 절화수명 Y=0.848X1+0.366X2-0.591X3+2.224X4- 0.171X5+0.47X6+0.321X7+9.836X8-110.219(X1-X8: 최고 RH, RH 일교차, DLI, pH, Hunter’s b value, EC, 절화 장, 잎 두께; R2=0.544)로 예측되었고, RWH 하우스의 절화수명 Y=-1.291X1+52.026X2-0.094X3+0.448X4-3.84X5+0.624X6 - 8.528X7+28.45(X1-X7: 경경, 야간 VPD, 최고 근권온도, 최 저 근권온도, 기온 일교차, RH 일교차, 최고 VPD; R2=0.5243) 로 예측되었다. 이 두 모델식으로부터 SC 하우스에서는 RH, EC 및 pH가, 그리고 RWH 하우스에서는 근권 온도가 절화수명에 더 큰 영향을 미친다는 것을 추론할 수 있다. 따라서 각 재배 방법에 따라 장미의 절화수명에 더 큰 영향을 미치는 환경적 요인을 효율적으로 관리할 필요가 있다.

Background: Recently, the single-step genomic best linear unbiased prediction (ssGBLUP) method, which incorporates not only genomic information but also phenotypic information of pedigree, is under study. In this study, we performed a ssGBLUP analysis on a commercial Hanwoo population using phenotypic, genotypic, and pedigree data. Methods: The test population comprised Hanwoo 1,740 heads raised in four regions of Korea, while the reference population used Hanwoo 18,499 heads raised across the country and two-generation pedigree data. Analysis was performed using genotype data generated by the Hanwoo 50 K SNP beadchip. Results: The mean Genome estimated breeding values (GEBVs) estimated using the ssGBLUP methods for carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), and marbling score (MS) were 7.348, 1.515, -0.355, and 0.040, respectively, while the accuracy of each trait was 0.749, 0.733, 0.769, and 0.768, respectively. When the correlation analysis between the GEBVs as a result of this study and the actual slaughter performance was confirmed, CWT, EMA, BFT, and MS were reported to be 0.519, 0.435, 0.444, and 0.543, respectively. Conclusions: Our results suggest that the ssGBLUP method enables a more accurate evaluation because it conducts a genetic evaluation of an individual using not only genotype information but also phenotypic information of the pedigree. Individual evaluation using the ssGBLUP method is considered effective for enhancing the genetic ability of farms and enabling accurate and rapid improvements. It is considered that if more pedigree information of reference population is collected for analysis, genetic ability can be evaluated more accurately.

식물의 흡수를 통한 공기오염물질 제거는 생육 상태에 따라 그 효과가 달라진다. 실내에서 토양수분의 공급은 식물의 생 육을 위한 기본적인 관리 사항이다. 따라서 본 연구는 토양수 분함량에 따른 생리적 반응이 가스상 공기오염물질인 톨루엔 저감에 미치는 영향을 구명하고, 최적의 생육과 공기 정화 효 과를 위한 적정 토양수분함량을 찾고자 수행하였다. 이를 위 해 스파티필름과 파키라를 사용하여 40일 동안의 평균 토양 수분함량을 25%, 20%, 15%, 10%로 처리한 후 양자수율, 광 합성률, 기공전도도, 증산량 등 생리적 지수와 엽면적당 톨루 엔 저감량을 측정하였다. 그 결과 스파티필름은 토양수분함량 을 20~25%로 관리할 때 생육이 양호하고 최적의 톨루엔 저 감 효과를 얻을 수 있을 것으로 판단되며, 10% 이하 건조에 대한 주의가 요구된다. 반면 파키라는 토양수분함량 20% 이 하 처리구에서 톨루엔 저감량이 증가하였으나 10% 처리구에 서 생장량이 저하될 가능성이 있으므로, 공기 정화와 생육을 위한 최적 토양수분함량은 15~20% 범위이며, 25% 이상으로 장기간 유지하는 것은 과습을 유발할 가능성이 있는 것으로 판단된다.

Background: Estrus in cows can be detected through vaginal electrical resistance or conductivity. However, there are no studies measuring vaginal electrical resistance in Hanwoo cows. This study aims to measure the vaginal electrical resistance value in Hanwoo cows and compare it with estrus and ovulation. Methods: Vaginal electrical resistance values of 73 Hanwoo cows were measured before and after estrus at the Gyeongsangbuk-do Livestock Research Institute. Measurements were taken on days -6, -3, -2, -1, 0, 1, 2, 3, and 6 of artificial insemination. Large follicles and ovulation were confirmed using transvaginal ultrasonography. Results: The vaginal electrical resistance averaged 225.6 ± 6.3 Ω days before the artificial insemination date, decreasing until the day of artificial insemination. The average vaginal electrical resistance was 163.7 ± 4.6 Ω on the date of artificial insemination, and 188.8 ± 4.3 Ω one day after artificial insemination, when large follicles were observed. In addition, on the 6th day after artificial insemination, the vaginal electrical resistance averaged 231.4 ± 5.5, which was similar to the 6th day before artificial insemination (222.5 ± 6.3). Transvaginal ultrasonography showed that most of the cows ovulated one day after artificial insemination. Conclusions: The accuracy of estrus is high if the vaginal electrical resistance is measured for cows with confirmed estrus, making is a potentially useful for determining the timing of artificial insemination.

자생 부추속 식물 중 강부추(Allium thunbergii for. rheophytum ined.)와 갯부추(A. pseudojaponicum Makino)는 관상용, 식 용 및 약용자원으로 가치가 있으나 육묘를 위한 생육환경조건 구명이 미비하여 연구할 필요성이 있다. 본 연구는 강부추와 갯부추의 육묘에 미치는 플러그 셀 크기, 차광률, 시비처리에 따른 영향을 구명하기 위하여 실험을 수행하였다. 강부추와 갯부추를 육묘한 결과, 플러그 셀 크기에서는 50, 72, 105, 128, 162, 200셀 처리 중 용적이 가장 큰 50셀에서 초장, 엽 수, 근수, 그리고 근장의 생육이 우수하였다. 그러나 생산비용 과 플러그 육묘의 효율성을 고려하여 105셀 이상의 플러그 트레이 중에서 선택하여 육묘하는 것이 효과적이라 판단된다. 차광률에 따른 유묘는 0, 30, 60, 90% 처리 중 30~60% 차 광처리에서 초장, 근수, 그리고 근장이 유의적으로 높게 측정 되어 생육이 양호하였다. 시비처리에서 생중량과 건중량을 제 외한 생육지표를 검토했을 때, 강부추의 적정 시비처리는 속 효성 고형비료(DO-PRO) 0.1g, 갯부추는 속효성 액체비료 (Peters) 주 1회 8mL 엽면시비처리였고 두 종 모두 속효성 시비처리가 효과적이었다. 강부추와 갯부추의 초기 생육에는 30~60% 차광처리가 된 재배플롯에서 원예상토가 충진된 128셀 플러그 트레이에 종자를 파종한후, DO-PRO 0.1g 또 는 Peters 8mL를 주 1회 엽면시비하면서 재배하는 것이 효 과적이라 판단된다.

This study aimed to compare the effects of self-regulation, goal orientation, and speaking anxiety on speaking performance between metaverse and face-to-face contexts. We randomly assigned 253 Korean middle school students to either metaverse or face-toface groups for 12 weeks of English-speaking lessons. Before and after the experiment, students completed speaking tests and submitted a post-questionnaire. Structural equation modeling and multi-group analysis revealed that student attributes had a similar impact on speaking performance in both settings. Specifically, self-regulation and mastery goal orientation positively influenced speaking performance, while speaking anxiety had a negative effect, regardless of the context. Furthermore, self-regulation played a mediating role in reducing speaking anxiety in both settings. This implies that metaverse-mediated and face-to-face classes offer similar learning environments, where students can leverage their goal orientation and self-regulation skills to manage speaking anxiety. Ultimately, students can enhance their speaking performance by employing selfregulation strategies and nurturing a mastery goal orientation, irrespective of the learning context.

This study was conducted to develop a seed propagation protocol of Hemerocallis taxa native to the Korean Peninsula. In this study, the seed dormancy types were classified. The germination traits of H. thunbergii, H. dumortieri, H. hakuunensis, and H. hongdoensis were also characterized. The initial embryo to seed length ratio (E:S ratio) in seeds of the four Hemerocallis species was between 0.80 and 0.90 at harvest, however, the E:S ratio did not increase until just before germination. Thus, seeds of the four Hemerocallis species had no morphological dormancy (MD), and at least 84% of seeds of all tested species germinated at 25/15℃ under light within 30 days. The lower the incubation temperature, the lower the germination rate of seeds of the four Hemerocallis species. However, the temperature range for germination was expanded due to old stratification. Gibberellin treatment increased the germination rate or germination speed at a relatively low temperature of 15/6℃. However, this treatment did not improve the germination rate as much as the temperature at 25/15℃. Under 15/5℃ of incubation, cold stratification increased the final germination percentage and rate/speed and expanded the temperature range for germination from high to low (25/15℃ → 15/6℃). This indicates that the seeds have a conditional dormancy (CD) that germinates in a narrow temperature range. In conclusions, the four Hemerocallis seeds exhibited nondeep physiological dormancy.

Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.