Hopyeongbyeo is an anther culture derived Japonica rice (Oriza sativa L.) cultivar from the cross between Hitomebore and Hwajinbyeo by the rice breeding team of Honam Agricultural Research Institute (HARI), NICS, RDA, in 2003. This cultivar has a about 126 days growth duration from transplanting to harvesting. The short and round grain shape of Hopyeongbyeo is translucent with non-glutinous endosperm. Amylose content of rice grain is about 17.8% and high palatability of cooked rice compared with Nampyeongbyeo. This cultivar shows resistant to bacterial blight pathogenes of K1. The milled rice yield of Hopyeongbyeo is about 5.13MT/ha at ordinary transplanting culture in the local adaptability test. Hopyeongbyeo would be adaptable for the southern plain area of Korea.

‘Gwangan’ is a new six-rowed covered barley cultivar developed by the National Institute of Crop Science (NICS), R.D.A in 2004. This cultivar is developed from a cross between “Mirak” and ‘Milyang59’ in 1995. An F6 selection was made at Milyang in 2001 an

A sequential planting method was developed to identify durable blast resistance in Korean rice cultivars. Two types of rice cultivars were investigated by a sequential planting after inoculation of mixed 26 rice blast isolates. Daesanbyeo and Gihobyeo, sh

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains 5~textrmmgL-1 kinetin, 0.5~textrmmgL-1 NAA, 2 % sucrose (or maltose), 3% sorbitol, and 500~textrmmgL-1 proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

N fertilizer required by rice could be reduced greatly in the rice-barley double cropping system than in the rice single cropping system. This study was conducted to investigate how much of the N fertilizer during the early stage of rice in the rice-barley double cropping system, could be saved compared to that in the rice single cropping system. This experiment was carried out at the paddy field of the National Crop Experiment Station in Suwon, Korea during three years from 1999 to 2001. Amounts of soil mineral nitrogen (SMN) and SPAD values of rice leaf during rice growing season in the rice-barley double cropping system were higher than those in the rice single cropping system under the same amount of N application during two years. Yield and N uptakes of rice at harvesting time were also higher in the rice-barley double cropping system than in the rice single cropping system during two years. Yield and N uptake of rice in the rice single cropping system were decreased when basal N fertilizer was omitted, but those reductions were not found by either omitting basal N fertilizer or omitting N fertilizer at tillering stage in the rice-barley double cropping system during 2000 and 2001. But yield and N uptakes of rice were decreased by 70 kg/10a and 2kgN/10a by the omission of both N application at basal and tillering stages in the rice-barley double cropping system in 2002. It was concluded that N fertilizer as much as tillering N fertilizer could be saved in the rice-barley double cropping system.

본 시험은 참깨 발병(병원균 : Phytophthora nicotianae var. parasitica)에 대한 품종저항성 검정방법 확립을 위해 실시하였다. 공식작물의 묘령, 접종방법, 토양수분상태 및 접종원의 농도는 공시한 중도저항성계통 B-67, IS 103과 나병성계통 수원9호, 수원 2006 모두의 병진전속도와 발병심도에 영향을 주는 것으로 나타났다. 그러나, 파종후 20일된 유묘에 1 m 당 200개 포자상의 접종원을 폿트(8 16.7 7.2cm)당 10m 씩 토양표면에 접종한 후 저면관수로 토양수분을 포화상태로 유지하였을 때 계통간 저항성의 차이를 갈 볼 수 있었다. 묘령 70일의 성묘를 사용하였을 때는 토양접종을 하였을 때나 분무접종을 하였을 때 모두 계통간 저항성 차이를 잘 볼 수 있었다. 전술한 유묘검정방법에 의해 이병성계통인 수원2006는 이병주율로는 100%, 발병심도로는 최대치인 9.0을 보였다. 반면에 중도저항성계통인 B- 67은 이병수율로는 20%, 발병심도로는 1.7을 보였다. 발병심도는 0에서 9까지 지수화하여 나타내었다. 예를 들면, 발병심도 0은 병징이 없는 상태, 5는 지면부 줄기가 변색된 경우, 9는 잎마름증상과 함께 줄기변색이 지면부로 부터 10cm 이상인 경우이다. 품종간 저항성의 차이는 병총면적률로도 조사를 하였으며, 이 결과는 발병심도로 조사한 검정결과와 잘 일치하였다.