This study was conducted to investigate the effect of microbial inoculation as additive consisting of novel lactic acid bacteria on quality and fermentation characterization of Miscanthus sinnensis silages.The contents of crude protein, acid detergent fiber, neutral detergent fiber in treatments of additive of lactic acid bacteria (ALAB) inoculation had similar to control. pH of Miscanthus sinnensis (MS) silage in treatments of ALAB inoculation significantly decreased as compared to control (p<0.05). The content of lactic acid in in treatments of ALAB inoculation significantly increased (p<0.05), but the content of acetic acid in treatments of ALAB inoculation decreased. In addition, number of lactic acid bacteria in treatments of ALAB inoculation significantly increased as compared to control (p<0.05). Therefor, we suggest that MS silage improved by inoculation of additive consisting of novel lactic acid bacteria.

This study was conducted to find out the effects of over seeding using new grass varieties orchardgrass ‘Kordione’ and ‘Onnuri’, and tall fescue ‘Greenmaster’ on botanical composition and dry matter productivity from 2013 to 2016 in low productivity hilly pasture, middle area of Korea. There were used 3 grass seed mixture types made of different compositions and amounts {T1: control - no over seeding, T2: tall fescue (TF) ‘Greenmaster’ 18 kg ha-1, orchardgrass (OG) ‘Kordione’ 9 kg ha-1, Perennial ryegrass (PRG) ‘Linn’ 5 kg ha-1, and Kentucky bluegrass (KBG) ‘Kenblue’ 2 kg ha-1, T3: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Kordione’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘ Kenblue’ 2 kg ha-1, T4: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Onnuri’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘Kenblue’ 2 kg ha-1}. In the botanical composition of grassland, T1 nearly remained constant in other seasons while ratio of weeds were increased in summer season. T2 was better than control (T1) in portion of grass ratio, which has increased by 80%. In the early time of establishment, the portions of OG were increased in T2, but TF rate was increased after 3 years later when grass was established. T3 and T4 showed a very similar patterns, grass ratio had increased by 80% and the portion of KBG had increased as time passed. T2 (129,763 kg ha-1) was showed the highest dry matter yield than other treatments (T1: 6,756 kg ha-1, T3: 9641, and T4: 10,738) in 2016.

This study was carried out to determine the effects of application levels of fertilizer and sowing methods on yields and nutritive values of Italian ryegrass (IRG) in early spring. Five fertilizer levels were used: Treatment 1, 100-80-80 kg/ha; Treatment 2, 120-100-100 kg/ha; Treatment 3, 140-120-120 kg/ha; Treatment 4, 160-140-140 kg/ha; Treatment 5, 140-120-120 kg/ha of N-P2O5-K2O with silicate fertilizer 200 kg/ha. Dry matter (DM) yield was 8,330 kg/ha in Treatment 5, 7,686 kg/ha in Treatment 4, and 7,347 kg/ha in Treatment 3. There was no significant difference in total digestible nutrients (TDN) content. The content of crude protein was the highest in Treatment 5. Dry matter ratio was the lowest in Treatment 5. In Treatment 3, DM yield was 7,347 kg/ha, when total amounts of fertilizers were applied at one time. However, DM yield was 7,405 kg/ha, when 50% of pre-planting fertilizer and 50% of supplementary fertilizer were applied at different time. There was no significant difference between total application and split application of fertilizers. However, DM yield was 9,469 kg/ha in application treatment with 100 kg/ha of additional urea at three to four leaf stages of IRG. Regarding DM yield by sowing methods of IRG, the following order was found: drill seeding (8,176 kg/ha) > rotary-broadcast seeding-stamping (7,957 kg/ha) > rotary-broadcast seeding (7,810 kg/ha) > broadcast seeding (7,347 kg/ha) > broadcast seeding-rotary (7,034 kg/ha). DM yield (59.57%) was the lowest in broadcast seedingrotary. Crude protein content was the highest with rotary work but the lowest with broadcast seeding.

This study evaluated the effect 3 types of conditioning (Untreated, Impeller, Roller) and the number of times for tedding (rake-teder) performed in one day on the drying rate of tall fescure hay. The experiment was performed at The forage site of Cheonan-si seongwhan-eup National Institute of Animal Sciencee. It took 3 days to make tall fescue hay. The Impeller and Roller conditioning types shortened the haymaking period by 1 day compared to untreated hay. Impeller and roller samples had less than 20% of water content by the 23rd-day at 13 o’clock. Tedding frequency failed to affect haymaking. There was no correlation between the three types of conditioning or tedding frequency and acid digestible fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDF), crude protein (CP), or relative feed value (RFV) in tall fescue hay. CP was 12.5~12.9% for the three types of conditioner with 1 or 2 times of tedding a day, Threrfore, conditioner types and gedding frequency had no significant effect on CP.

This study was conducted to determine the effect of rainfall after cutting on nutritive value of Italian ryegrass (Lolium multiflorum) hay. Fresh Italian ryegrass was treated with 3 days natural rainfall (56.5, 14.0 and 1.6 mm) after cutting without tedding. Rainfall effect on Italian ryegrass quality was evaluated based on Neutral detergent fiber (NDF) and acid detergent fiber (ADF), crude protein (CP), crude fat (CF), crude ash (CA) and non-fiber carbohydrate (NFC). The quality of Italian ryegrass was decreased with extending raining days. The content of neutral detergent fiber (NDF) was increased from 0.4 to 4.7% by 1 day advancement in raining. CF content was decreased from 8.1 to 32.8%. CP and CA contents did not change, but NFC content was decreased from 0.5 to 8.4%. The quality damage by the number of raining days after cutting Italian ryegrass was the lowest on the first day from exposure to rain.

This study was conducted to determine the effect of mathematical transformation on near infrared spectroscopy (NIRS) calibrations for the prediction of chemical composition and fermentation parameters in corn silage. Corn silage samples (n=407) were collected from cattle farms and feed companies in Korea between 2014 and 2015. Samples of silage were scanned at 1 nm intervals over the wavelength range of 680~2,500 nm. The optical data were recorded as log 1/Reflectance (log 1/R) and scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares (PLS) multivariate analysis in conjunction with several spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation (R2 cv) and the lowest standard error of cross validation (SECV). Results of this study revealed that the NIRS method could be used to predict chemical constituents accurately (correlation coefficient of cross validation, R2 cv, ranging from 0.77 to 0.91). The best mathematical treatment for moisture and crude protein (CP) was first-order derivatives (1, 16, 16, and 1, 4, 4), whereas the best mathematical treatment for neutral detergent fiber (NDF) and acid detergent fiber (ADF) was 2, 16, 16. The calibration models for fermentation parameters had lower predictive accuracy than chemical constituents. However, pH and lactic acids were predicted with considerable accuracy (R2 cv 0.74 to 0.77). The best mathematical treatment for them was 1, 8, 8 and 2, 16, 16, respectively. Results of this experiment demonstrate that it is possible to use NIRS method to predict the chemical composition and fermentation quality of fresh corn silages as a routine analysis method for feeding value evaluation to give advice to farmers.

In order to provide basic data to predict changes of milk components in milk yield, this study was conducted to compare nutritive values of diets collected from organic dairy farms (ODF) and conventional dairy farms (CDF) of Chungnam and Jeonbuk in Korea. The contents of crude fat, crude fiver, neutral detergent fiber, and acid detergent fiber in diets of ODF were significantly (p<0.05) higher compared to those of CDF. The contents of crude protein and crude ash in ODF were also higher (p<0.05) than those of CDF. The contents of calcium, ferrum, and phosphorus in diets of ODF were similar to those of CDF. The contents of potassium, magnesium, and iodine in diets of ODF were higher (p<0.05) than those of CDF. The contents of total saturated and unsaturated fatty acids in diets of ODF were higher (p<0.05) compared to those of CDF. The contents of oleic acid, linoleic acid, and linolenic acid in diets of ODF were higher (p<0.05) compared to those of CDF. However, the contents of myristic acid, palmitic acid, and stearic acid in diets of ODF were lower (p<0.05) compared to those of CDF.

The present study was carried out to explore the potential effects of novel lactic acid bacteria Pediococcus penticeous KCC-23 (KCC-23) and Lactobacillus plantarum KCC-24 (KCC-24) on rye haylage fermentation at National Institute of Animal Science, Cheonan province in Korea. The experiment contains three different groups such as control without lactic acid bacteria, rye haylage with KCC-23 and rye haylage KCC-24. After experimental periods, the content of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), the activity of total digestible nutrient (TDN) and In-vitro digestibility (IVD) was similar in control and LAB treated haylage (p<0.05). The pH was reduced in KCC-23 and KCC-24 treated rye haylage as compared to control (p<0.05). The lactic acid concentration in haylages was increased by L. plantarum KCC-23, and KCC-24 supplement. Whereas, the less amount of acetic acid and butyric acid was noted in KCC-23 and KCC-24 treated haylage as compared with control (p<0.05). The KCC-23 and KCC-24 were dominantly grown in experimental haylage as compared with control. It indicates, the addition of KCC-23, and KCC-24 enhances fermentation quality of haylages as compared control. The present study suggests that KCC-23 and KCC-24 are potent strains that were improving the fermentation process in rye haylage

The growth of Italian ryegrass (IRG) after wintering was very low in 2015 when IRG was broadcasted under growing rice in fall of 2014. To determine growth inhibitory factors of IRG, we examined the growth conditions of IRG in Nonsan region and meteorological conditions in Daejeon nearby Nonsan. Minimum temperature and maximum instantaneous wind speed on Feb. 8th and 9th of 2015 after wintering of IRG were 8.8℃, 10.7 m/s and 12.4℃, 9.6m/s, respectively. Air temperature was suddenly dropped due to strong wind with snow showers, which had unfavorable effect on root growth of IRG exposed at the soil surface. The minimum temperature and maximum instantaneous wind speed on Feb. 12th, 13th, and 14th of 2015 were 4.1℃, 11.6 m/s, － 5.6℃, 10.3 m/s, and －4.7℃, 7.5 m/s, respectively. The growth circumstance of IRG was not good because soil was dried due to drought continued from January. The minimum temperature and maximum instantaneous wind speed on Feb. 26th, 27th, and 28th of 2015 were 1.8℃, 13.7 m/s, －3.5℃, 10.6 m/s, and 4.1℃, 6.8 m/s, respectively. The number of wilting of IRG was more than 59% until Mar. 3rd of 2015. IRG faced irreparable environment (low minimum temperatures and extreme instantaneous wind speeds) for 9 days from Mar. 4th to Mar. 12th of 2015. The main reason for the decrease of IRG productivity was collection delay of rice straw after rice harvest because there was continuous rain between Oct. and Nov. of 2014. For this reason, weakly grown IRG under rice straw was withered after wintering. IRG was withered by frost heaving, drought, and instantaneous wind speed in the spring. Furthermore, the root of IRG was damaged while growing in excess moisture in the surface of paddy soil during the winter season due to rain.

본 연구는 2014 / 2015년 동계 사료작물의 월동 후 생육 조사를 통하여 전국적인 작황을 파악함으로서 사료작물 생 육에 문제점이 있을시 근본 원인을 구명할 수 있는 기초자 료로 이용하거나 조사료의 연중 안정 생산, 공급에 도움을 주고자 수행하였다. 2015년도 전국 동계 사료작물의 월동 후 생육 상황은 전반적으로 저조하였는데, 월동률 분포에 따른 지역별 분포 상황을 살펴보면 월동률 80% 이상인 지 역이 66%, 월동률 79~50% 분포지역이 24.9%, 50%미만 지 역이 9.1%의 지역별 분포를 나타내어 전체적으로는 79%의 월동률을 나타냈다. 월동 후 월동률 및 피복률은 배수로가 설치된 논에서는 각각 83%와 80%로서 양호했으나 배수로 가 설치 안 된 습한 논에서는 각각 67%와 66%로 낮은 경 향을 나타냈다. 전국 동계작물 조사료 생산량은 강원, 충 북, 충남, 경남, 전남지역은 10~15%, 경기, 경북, 전북지역 은 약 30%의 수량감소가 예상되어 전국적으로 약 19%의 수량 감소가 예상되었다.

본 연구에서는 호밀을 이용하여 사일리지 제조시 Lactobacillus plantarum KCC-10 및 KCC-19를 접종하여 사일리지의 사 료가치, 품질 및 미생물상의 변화를 조사하였다. 호밀 사일 리지에서 젖산균 접종구와 무접종구에서 사료가치는 크게 변화되지 않았다. 그리고 유산 함량은 젖산균 접종구가 현 저하게 높은 경향을 보였으나 초산과 낙산은 젖산균 접종 구가 약간 낮아지는 경향을 보였다. 젖산균 분포는 젖산균 접종구에서 현저하게 높았으며 효모와 곰팡이는 104CFU/g 에서 관찰되지 않았다. 호밀 사일리지 제조시 KCC-10 및 KCC-19 접종구의 조단백질 및 TDN 함량은 무접종구 보 다 약간 증가하였으나 섬유소 및 in vitro 건물소화율에서 는 비슷한 수준을 보였다. 이상의 결과를 요약해 보면 호 밀 사일리지 제조시 KCC-10 및 KCC-19 접종에 의해 사일 리지의 품질이 향상되었다.