Fusarium head blight (FHB) is the most important disease of wheat that may cause serious yield and quality losses and leads to harmful contamination of the grain with fungal toxins. Korean wheat cultivars showed much less resistant to FHB than Sumai3, which known as resistance to FHB, evaluated by spray inoculation in our companion report. Many DNA-marker studies, including sequence tagged site (STS) and simple sequence repeat (SSR) markers and qualitative trait loci (QTL) associated with FHB resistance have been identified and mapped on the specific chromosome, especially 3BS, by many previous studies. However, there was no application of DNA-markers to select FHB resistance lines in Korean wheat breeding programs. 3 SSR markers and 6 STS markers linked to major QTL on chromosome arms 3BS found in Sumai3 and its derivatives were used to screen FHB resistance in Korean wheat cultivars. All markers known to be most significant markers based on previous research (Liu and Anderson 2003). There was general lack of marker polymorphism for Korean wheat cultivars used in this study. However, STS3B-138 marker only showed the polymorphism in Korean wheat cultivars. Allele size of STS3B-138 is 355 bp in Sumai 3 and 12 of 24 cultivar showed this allele. The range in Type II resistance rating was 1-5 and average of these 12 cultivars have 355bp allele was grade 2.4. The most resistant cultivars under the 2 grade, Gobun, Jinpoom, Milseong and Namhae also showed 355bp allele. Therefore, These results provide potential for development of wheat cultivars with improved Fusarium head blight resistance through use of effective FHB screen methods and marker - assisted selection.

This paper describes the Flight Dynamics Automation (FDA) system for COMS Flight Dynamics System (FDS) and its test result in terms of the performance of the automation jobs. FDA controls the flight dynamics functions such as orbit determination, orbit prediction, event prediction, and fuel accounting. The designed FDA is independent from the specific characteristics which are defined by spacecraft manufacturer or specific satellite missions. Therefore, FDA could easily links its autonomous job control functions to any satellite mission control system with some interface modification. By adding autonomous system along with flight dynamics system, it decreases the operator’s tedious and repeated jobs but increase the usability and reliability of the system. Therefore, FDA is used to improve the completeness of whole mission control system’s quality. The FDA is applied to the real flight dynamics system of a geostationary satellite, COMS and the experimental test is performed. The experimental result shows the stability and reliability of the mission control operations through the automatic job control.

One of the most extensively studied populations of multipotent adult stem cells are mesenchymal stem cells (MSCs). MSCs derived from the human umbilical cord vein (HUC-MSCs) are morphologically and immunophenotypically similar to MSCs isolated from bone marrow. HUC-MSCs are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. Since neural tissue has limited intrinsic capacity of repair after injury, the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesenchymal-like stem cells from the human umbilical cord vein, and studied transdifferentiation-promoting conditions in neural cells. Dopaminergic neuronal differentiation of HUC-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulfoxide (DMSO) and butylated hydroxyanisole (BHA) in N2 medium and N2 supplement. The immunoreactive cells for -tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. HUC-MSCs treated with bFGF, SHH and FGF8 were differentiated into dopaminergic neurons that were immunopositive for tyrosine hydroxylase (TH) antibody. HUC-MSCs treated with DMSO and BHA rapidly showed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including NeuroD1, -tubulin III, GFAP and nestin was markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after neural differentiation, we confirmed the differentiation of dopaminergic neurons by TH/-tubulin III positive cells. In conclusion, HUC-MSCs can be differentiated into dopaminergic neurons and these findings suggest that HUC-MSCs are alternative cell source of therapeutic treatment for neurodegenerative diseases.

The field of vehicle routing is currently growing rapidly because of many actual applications in truckload and less than truckload trucking, courier services, door to door services, and many other problems that generally hinder the optimization of transportation costs in a logistics network. The rapidly increasing number of customers in such a network has caused problems such as difficulty in cost optimization in terms of getting a global optimum solution in an acceptable time. Fast algorithms are needed to find sufficient solutions in a limited time that can be used for real time scheduling. In this paper, the nearest L-method (NLNM) is proposed to obtain a vehicle routing solution. String neighbors of different lengths were chosen, tested and compared. The applied de crossing procedure is meant to solve the routes by NLNM by giving a better solution and shorter computation time than that of NLNM with long string neighbors.

To tolerate environmentally adverse conditions such as cold, drought, and salinity, plants often synthesize and accumulate proline in cells as compatible osmolytes. δ1 -Pyrroline-5-carboxylate synthetase(P5CS) catalyzes the rate-limiting step of proline biosynthesis from glutamate. Two complete genes, MtP5CS1 and MtP5CS2, were isolated from the model legume Medicago truncatula by cDNA cloning and bacterial artificial chromosome library screening. Nucleotide sequence analysis showed that both genes consisted of 20 exons and 19 introns. Alignment of the predicted amino acid sequences revealed high similarities with P5CS proteins from other plant species. The two MtP5CS genes were expressed in response to high salt and low temperature treatments. Semi-quantitative reverse transcription-polymerase chain reaction showed that MtP5CS1 was expressed earlier than MtP5CS2, indicating differential regulation of the two genes. To evaluate the reverse genetic effects of nucleotide changes on MtP5CS function, a Targeting Induced Local Lesions in Genomes approach was taken. Three mutants each were isolated for MtP5CS1 and MtP5CS2, of which a P5CS2 nonsense mutant carrying a codon change from arginine to stop was expected to bring translation to premature termination. These provide a valuable genetic resource with which to determine the function of the P5CS genes in environmental stress responses of legume crops.

“Yeonganbyeo”, a new japonica rice variety (Oryza sativa L.), is a mid- maturing ecotype with high lysine content in kernels that was developed by the rice breeding team of National Yeongnam Agricultural Experiment Station (NYAES) in 2001 and released in 2002. This variety was originated from the single cross of Milyang 122/YR13616 Acp1 (in 1992/1993 winter) and was selected by means of a mixed method of bulk and pedigree breeding. The pedigree of “Yeonganbyeo” was YR15815-B-B-B-30 and designated in 1998 as “Milyang 164”. It has about 83cm in culm length with lodging tolerance. This variety is resistant to bacterial leaf blight (K1, K2, K3), stripe virus, and moderately resistant to leaf blast disease. Milled rice kernels of “Yeonganbyeo” has high lysine content of 4.31% (ratio of amino acid components in total protein), a clear translucent with non-glutinous endosperm and clear in chalkness and good at eating quality by pannel test. The yield potential of “Yeonganbyeo” in milled rice is about 5.45 MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to the southern plain of Korea.