In the 21st century, the risk of the exotic pest being invaded is increasing due to the rise of trading activities. Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), has been considered to very destructive invader as it is highly intrusive and has a wide host range. In this study, 2,299 samples were collected from 88 locations in 12 countries by quarantine and investigation. Among them, 608 individuals (including B. correcta) were used for COI DNA barcoding analysis based on Neighbor Joining method with P-distance model. Population genetics analysis was conducted for 510 individuals selected from 47 locations of 12 Southeast Asian countries using 15 microsatellite loci. The barcoding analysis resulted in that any clade was not clustered according to a geographical isolation but indicated geographically mixed populations. Population genetics analysis showed shared genetic structure between neighboring countries across borders. Genetic structure of most Korean quarantine groups was more similar to that of Taiwan, China and Thailand in the order of appearance than other countries.

A species of Beauveria bassiana is widely used for biological pest management in many countries. Many efforts have been given to figure out the clear fungal mode of action to enhance the insecticidal activity. Homologous recombination (knock-out) or hairpin RNA (knock-down) is popularly used in fungal gene function study, but gene cloning and generation of knock-out or -down mutants takes long time or temporarily knock-downed. Here in this work, we used previously generated egfp-expressing B. bassiana strain (Bb-egfp #3) and integrated dsegfp to the Bb-egfp #3 using a protoplast integration method. This work suggests that protoplast integration with dsRNA possibly generate significantly reduced gene expression in B. bassiana and the reduction is quite stable over generations which provide easy of functional study for fungal mode of action.

With industrialization, environmental problems have become severe worldwide as resources are exhausted for mass production purposes and pollutants are produced in excess of nature's capacity to absorb them. Since modernity, urban parks have emerged as an important element for addressing challenges facing urban environments, which include environmental degradation. In 1987, the Brundtland Commission’s report presented a sustainable developmental perspective in solving environmental problems and provided ideas for sustainable design and sustainable urban park landscape design. The purpose of this study is to analyze sustainable design applied to Houtan Park in central Shanghai, China from the perspective of social culture preservation, ecological restoration, and economic effect, and to provide the basic data for urban park design in similar areas in the future. This study consists of a literature survey and a field investigation. The field investigation lead to analysis from three perspectives: social culture preservation, ecological restoration, and economic effect. The literature survey examined sustainable urban parks and Houtan Park based on relevant papers, newspaper articles, and reports. Through actual visits (Aug. 28 - Sep. 1, 2018), it also examined the elements of sustainable design that were applied to the architecture. The three sustainable design elements derived from this study, being a sustainable design for the future, will be used as an important basis for developing urban parks for the regeneration of brownfields in many areas in China.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Recently, polymorphisms reported to be significant association with sperm MOT. This study was conducted to evaluate the SNP in the coding region of ESR1 (g.672C>T inexon 1) as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results,The g.672C>T was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP Also, the SNP was low significantly associated with ALH.Therefore, we suggest that theSNP in the coding region of ESR1 (g.672C>T in exon 1) may be used as a molecular marker for Duroc boar Post-thawed semen quality.

Estrogen receptor 2 (ESR2) is involved in oestrogen-related apoptosis in cell cycle spermatogenesis but their effects have not yet confirmed in pig. Therefore, this study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 126 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were used in present study. A single nucleotide polymorphism (g.35547A>G) was associated with MOT, VCL, VAP and ALH in Duroc population (p < 0.05). Therefore, we suggest that the porcine ESR2 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the ESR2 in spermatogenesis within the reproductive tracts, and will shed light on ESR2 as a candidate gene in the selection of good sperm quality boars.

The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.

Insect-killing fungi have high potential in pest management. A deeper insight into the fungal genes at the whole genome level is necessary to understand the inter-species or intra-species genetic diversity of fungal genes, and to select excellent isolates. In this work, we conducted a whole genome sequencing of Beauveria bassiana (Bb) JEF-007 and characterized pathogenesis-related features and compared with other isolates including Bb ARSEF2860. A large number of Bb JEF-007 genes showed high identity with Bb ARSEF2860, but some genes showed moderate or low identity. The two Bb isolates showed a significant difference in vegetative growth, antibiotic-susceptibility, and virulence against Tenebrio molitor larvae. When highly identical genes between the two Bb isolates were subjected to real-time PCR, their transcription levels were different, particularly in heat shock protein 30 (hsp30) gene which is related to conidial thermotolerance. In several B. bassiana isolates, chitinases and trypsin-like protease genes involved in pathogenesis were highly conserved, but other genes showed noticeable sequence variation within the same species. Given the transcriptional and genetic diversity in B. bassiana, a selection of virulent isolates with industrial advantages is a pre-requisite, and this genetic approach could support the development of excellent biopesticides with intellectual property protection.

Origin of Red imported fire ant (RIFA : Solenopsis invicta) is Central America, a tropical climate region. It has settled in the invasive area, causing various problems. In recent, Solenopsis invicta were discovered in Busan in 2017 and then in 2018 at a construction site in Busan, Pyongtaek, Incheon and in Daegu. This study aims to confirm the origin of invasive colonies of Solenopsis invicta. We tried to test previously developed microsatellite markers so that we establish the tracing protocol for molecular epidemiology of RIFA. We justified 66 microsatellite markers already developed using DNA from the RIFA found in Incheon Harbor. As results, 30 markers were selected to facilitate amplification and fragment analysis.

The Asian corn borer, O. furnacalis (Crambidae) and the old world bollworm, H. armigera (Noctuidae) simultaneously cause ear damage in corn in Suwon area. It has been assumed that the second generation of larvae of both insects is responsible for the damage. We, therefore, studied proper spray timing of insecticide in order to reduce the damage. A waxy corn hybrid cultivar, Ilmichal, was sown directly on four plots of a field at April 10, April 20, May 4, and May 18, 2018, respectively. A sodium channel blocker, indoxacarb, was sprayed eight times at 3- or 4-day intervals around the silking stage of corn in each sowing plot. At ear harvest time of each plot, we investigated damaged ratio and length of ear, and the numbers of both insect species. The significantly effective spray timings on reduction of ear damage were the sprays at June 29 and July 2 in the plot sown at April 10, the sprays from July 2 to 13 in the April 20-sown plot, the sprays from July 2 to 19 in the May 4-sown plot, and the sprays at July 19 in the May 18-sown plot, respectively. The damage level was positively related to the insect number. The result indicated that the spray at the silking stage of corn was effective on reduction of ear damage. It was assumed that occurrence time of neonate larvae of the insects at silking stage cause the damage, based on the experimental results using screened pots inoculated with the two species.

2018년 현재까지 농림축산검역본부 호남지역본부로 수입된 식물은 220종 17,030건으로 우드펠렛(18%), 주정박(8%), 페스큐 건초(8%), 알파파건초(8%), 귀리건초(6%), 혼합건초(5%), 티모시건초(5%), 라이그래스건초(5%), 목화씨(사료용)(4%), 소나무원목(3%) 등의 순이다. 이와 같이 호남지역은 비재식 사료류(건초 등) 수입비중이 높으며 이는 전국 수입 건수의 약 67%에 달한다. 검역해충은 795건 검출되었으며 이 중 관리해충 294건, 잠정규제해충 146건, 비검역해충 307건 등이다. 관리해충 중 Carpophilus obsoletus가 210건(72%), 잠정규제해충 중 Cryptoletes ferrugineus가 56건(39%)으로 높은 검출율을 보였다. 또한 호남지역으로 수입 된 재식용 구근류는 생강 및 생강구근, 마늘구근이며 전국 수입 건수의 약 42%를 차지한다. 수입 된 생강 및 생강구근 91건 중 37건에서 해충(선충)을 발견하였고 Meloidogyne sp.(잠정규제해충) 검출로 9건이 폐기·반송되었다. 수입된 마늘구근 34건 중 29건에서 해충을 발견하였고 Carpophilus obsoletus(관리해충) 18건, Cryptoletes ferrugineus(잠정규제해충) 11건이 검출되어 소독하였다.

The continuous emergence of chemical-mediated residual toxicity and insect resistance have enforced the regulation of synthetic pesticides. Synthetic pesticides with novel mode of actions could be developed to overcome these issues, but as an alternative biopesticides with more efficacious control activity could be developed by the advanced technology. In pest management, entomopathogenic fungi have high potential in reducing pest population to an economic threshold, and some of isolates have been commercialized. However still a novel application strategy needs to be considered for successful industrialization. An insight on fungal genes in whole genome and transcriptome levels is necessary to understand the role of genes in pathogenesis and genetic diversity of fungal genes. Herein, we have identified the genetic differences of entomopathogenic fungi using whole genome sequencing of Beauveria bassiana (Bb) and tried to understand the interaction between fungus and insect using RNA-seq. We have obtained the whole genome sequence of Bb JEF-007 using PacBio sequencing technology and compared the transcriptomes of Bb JEF-007 and bean bug, Riptortus pedestris before and after the fungal infection using Hiseq 2000 system.