The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the NH4 + and CO2concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the CO2 and NH4 + concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing NH4 + excretion and O2 consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.

Background : Curcuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called turmeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation regions due to rise in average temperature. In order to select superior lines based on agronomic characteristics, we analyzed multivariate and estimated selection effects from C. longa germplasm. Methods and Results : The C. longa germplasm were cultivated in an experimental field located in Eumseong, NIHHS, RDA. The harvested roots were investigated in agronomic characteristics included in yield and then considered its relationship among the 9 germplasm by multivariate analysis method. Results from principal component analysis (PCoA) showed that it represented 70.00% and 80.44% accumulated explanation from four and five principal compounds (PC). PCoA was conducted from 9 agronomic characteristics and then correlation coefficient has been showed by analysis between each main component value and agronomic characteristics. Value of the first PC was 2.25, 24.96% explanation of total dispersion, plant height, number of rootlet and weight of rootlet were correlated with a somewhat higher level as 0.41, 0.43 and 0.52. Value of the fifth PC was 0.94, 10.43% explanation of total dispersion, the number of shoots was correlated with a higher level as 0.87. Selection effects with outstanding candidate lines including higher lines were estimated at 126.13% in yield. Conclusion : These data on multivariate based on agronomic characteristics will be give us invaluable breeding information by selection of superior lines.

Background : Cylindrocarpon root rot, caused by Cylindrocarpon destructans and Fusarium solani, is a major disease which lead to replanting failure in ginseng garden. Chlamydospores of C. destructans, generated by poor environmental condition, can survived more than ten years in soil without host plant, ginseng. Density of soil pathogens gradually decreased as the progress of time since ginseng was harvested. Methods and Results : Soil chemical properties was analyzed from soil samples of 43 regions in farmer’s ginseng garden to study the variation of content and the correlation among inorganic contents. Soil samples of 24 regions was also analyzed to study correlation between progressed-year after ginseng harvest and soil chemical properties. Variation of soil chemical properties in descending order was Fe, Zn, P2O4, NO3, Mn. The content of Fe and Zn were great variation among inorganic chemicals of soil of farmer’s field. Electrical conductivity to induce physiological demage in excessive concentration showed highly significant positive correlation with the content of NO3 and K. As the progress of year after ginseng harvest, the content of organic matter and zinc was increased, while pH and Na were decreased in farmer’s field to cultivate ginseng. There were highly significant positive correlation between the progress of year after ginseng harvest and zinc content in farmer’s field to cultivate ginseng. Ratio of root rot of 2-year-old ginseng showed significant positive correlation with K content, and negative correlation in experimental field cultured by six rotation crops for one year. Conclusion : Root rot by soil pathogens was closely related with the content of potassium and zinc in soil chemical properties.

Background : Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replanting failure in ginseng garden. Methods and Results : Solarization was done in the infested soil of the greenhouse for summer season (from July 24 to Autumn 31, 2014) after putting green manure (Sudan grass) and calcium cyanamide (CC) into the soil. Mycelium and conidia of C. destructans died at 4 0℃ after 15 hours, and 45℃ after 5 h, but it did not die at 35℃ after 15 h. Those of C. destructans died after keeping it for 2 hours daily at 40℃ for 9 days, and 45℃ for 8 days, but did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ in 5 cm depth, 48.7℃ in 10 cm, 44.7℃ in 15 cm, 42.5℃ in 20 cm, and 31.9℃ in 30 cm by putting green manure into the soil and solarization. Reduction of sudan grass increased electrical conductivity (EC), organic matter, P2O5, K, and Mg, while decreased pH, NO3-N, and Na. Addition of calcium cyanamide and urea gave a negative effect on the growth of ginseng because EC and NO3-N were increased excessively than the optimal range. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. But the root disease that occurred between single treatment of sudan grass and the treatment mixed with calcium cyanamide showed not a significant different. Addition of calcium cyanamide showed the decrease of root weight because leaves were dead early by a excessive increase of EC and NO3-N. Conclusion: Soil disinfection using green manure and solarization in greenhouse was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

Measurements of closely related sets of classical and truss dimensions were analyzed to discriminate species of scorpaenidae including the dark banded rockfish, Sebastes inermis, the black rockfish, S. schlegeli, and gobioninae including the striped shiner, Pungtungia herzi, and the slender shiner, Pseudopungtungia tenuicorpa. The measurements of the dimensions were arc sin square root transformed, and compared as a function of the standard length of each species for statistical analysis. For values of the classical dimensions of the rockfish, 6 were greater for the dark banded rockfish than for the black rockfish, 1 value was smaller for the former, and for 2 values there was no statistically significant difference (P > 0.05). For values of the classical dimensions of the shiners, 9 values were greater for the striped shiner than for the slender shiner, 2 values were smaller for the former, and for 1 value there was no statistically significant difference (P > 0.01). For values of the truss dimensions of the rockfish, 6 were greater for the dark banded rockfish than for the black rockfish, 1 was smaller for the former, and for 4 values there was no statistically significant difference (P > 0.05). For values of the truss dimensions of the shiners, 13 values were greater for the striped shiner than for the slender shiner, 3 values were smaller for the former, and for 6 values there was no statistically significant difference (P > 0.01). The dimension sets used in this study may be useful as taxonomic indicators for discriminating among fish species in Korea.

We examined the morphometric characteristics and fluctuating asymmetry of diploid and triploid marine medaka, Oryzias dancena. We used morphometric parameters the truss and classical dimensions. Significant differences in all the classical and truss dimensions of the diploid and triploid fish. All the dimensions of the triploid fish were greater than those of the diploid fish. The triploid marine medaka shows sexual dimorphism in these characters, and the sexual dimorphism of the triploid marine medaka is similar to that of the diploid marine medaka. Thus, the growth of triploid marine medaka is faster than that of the diploid fish, and it displays clear sexual dimorphism, with male fish having longer dorsal and anal fins than female fish. we examined fluctuating asymmetry of eye diameter, maxilla length, operculum length, number of pectoral fin ray and number of pelvic fin ray. In all experimental groups, Eye diameter and maxilla length showed no significant difference between left side and right side (P>0.05). Trends of operculum length in triploid male group and pectoral fin ray's number in diploid male group showed similar trend with trends of operculum length in triploid female group and pectoral fin ray’s number in diploid female group. However, trends of operculum length in diploid male group and pectoral fin ray's number in triploid male group showed opposite trend with trends of operculum length in diploid female group and pectoral fin ray’s number in triploid female group. Trend of pelvic fin ray's number in all groups showed similar trend with trend of pectoral fin ray’s number in all groups.

The bulb storability of eight red and ten yellow onion (Allium cepa L.) cultivars grown primarily in Korea was evaluated. During storage, sprouting occurred earlier in red cultivars than in yellow cultivars. In addition, the ratio of sprouted to unsprouted bulbs increased more rapidly in red cultivars than in yellow cultivars. However, not all yellow cultivars had strong storabilities. Bulb storability of three yellow cultivars was as poor as that of red cultivars, suggesting only a slight possibility of a pleiotropic effect of color and bulb storability. Meanwhile, F3 lines of a red cultivar selected based on stronger storabilities showed intermediate storabilities between those of the red and yellow cultivars, implying that strong storability could be obtained by successive selection. In contrast, F1 hybrids from crosses between yellow and red breeding lines showed poor storability compared with the yellow cultivars, indicating that poor storability may be dominant over strong storability. The relationships between color and other traits that potentially affect storability, such as fresh and dry weight, water content, and firmness of bulbs, were evaluated. No significant differences in these traits were observed between the two colors, although the water content of yellow cultivars and the dry weight of red cultivars were slightly higher than their counterparts. Correlation analyses between bulb storability and other traits, including weight, water content, and firmness, also showed no significant correlations. In this study, no correlations were identified between bulb color, storability, and other traits. However, based on these results, red cultivars with stronger storabilities could be developed through successive selection of bulbs with stronger storabilities.

A new poinsettia cultivar 'Scarlet' was bred by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Christmas Eve', a variety with dark green leaves and dark red bracts, and 'Red Velvet', a red colored variety in 2003. 'Scarlet' was finally selected in 2006 after investigating the growth and flower characteristics from 2004 to 2006. 'Scarlet' has bright red colored and elliptic bracts and has absent or very weak intensity of rugosity between veins in bract. In comparison with the check variety, 'Red Velvet', plant height is tall, leafblade is long and narrow. Petiole is also longer than that of 'Red Velvet'. Short day response is fast and its bracts are fully colored 7 weeks after short day commencement.

A new poinsettia cultivar 'Miss Maple' was bred by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between 'Sonora Red', a variety with short plant height, dark green leaves and dark red bracts, and 'Cranberry Punch', a deep pink colored variety in 2003. 'Miss Maple' was finally selected in 2006 after investigating the growth and flower characteristics from 2004 to 2006. 'Miss Maple' has light red colored and obovate bracts, and has weak intensity of rugosity between veins in bract. Fully colored transitional leaves are so deeply lobed that they resemble the leaves of maple. Plant height is shorter than that of 'Cranberry Punch', the check variety. Short day response fast and its bracts are fully colored 7 weeks after short day commencement.

“Jungsanbyeo” is a japonica rice cultivar developed from a cross between Sambaegbyeo and Milyang107 by Sangju Substation of National Yeongnam Agricultural Experiment station, RDA in 2000. The cultivar is early matured with heading date of July 30 ordinary

Munjangbyeo' is a japonica rice cultivar developed from a cross between Sangsanbyeo and Suweon 397 by Sangju Substation of National Yeongnam Agricultural Experiment Station, RDA in 1999. The cultivar is early matured with heading date of Aug. 2 in ordinar

Sangmibyeo' is a japonica rice cultivar developed from a cross between Sambaegbyeo and Ou 316 by Sangju Substation of National Yeongnam Agricultural Experiment Station, R.D.A. in 1998. The cultivar is early maturing with heading date of August 7 in ordina

A sweet sorghum [Sorghum bicolor (L.) Moench] cultivar was planted on 9 and 30 June 2000 at plant densities of 4, 6, 8, 10, and 12 plants m-2 to determine the optimum plant density in Jeju region. There were no significant planting date x plant density interactions for most traits measured. Delaying planting from 9 to 30 June delayed 21 days in heading date, and significantly decreased plant height, the number of productive stems m-2 /, and lodging. Fresh stem yield tended to be higher at the 9 June planting date than at the 30 June planting date, but total sugar and ethanol yields were not significantly affected by planting date. Percentage of soluble solid was higher at the 30 June planting date compared with the 9 June planting date. Fresh stem, total sugar, and ethanol yields quadractically increased from 22.9 to 36.7 t-1 , from 1.66 to 2.54 t-1 , and from 945 to 1440 L-1 , respectively, with increasing plant density. The optimal plant densities for the maximum fresh stem, total sugar, and ethanol were estimated to be 10.7, 9.6, and 9.9 plants m-2 respectively.