Background: Estrus in cows can be detected through vaginal electrical resistance or conductivity. However, there are no studies measuring vaginal electrical resistance in Hanwoo cows. This study aims to measure the vaginal electrical resistance value in Hanwoo cows and compare it with estrus and ovulation. Methods: Vaginal electrical resistance values of 73 Hanwoo cows were measured before and after estrus at the Gyeongsangbuk-do Livestock Research Institute. Measurements were taken on days -6, -3, -2, -1, 0, 1, 2, 3, and 6 of artificial insemination. Large follicles and ovulation were confirmed using transvaginal ultrasonography. Results: The vaginal electrical resistance averaged 225.6 ± 6.3 Ω days before the artificial insemination date, decreasing until the day of artificial insemination. The average vaginal electrical resistance was 163.7 ± 4.6 Ω on the date of artificial insemination, and 188.8 ± 4.3 Ω one day after artificial insemination, when large follicles were observed. In addition, on the 6th day after artificial insemination, the vaginal electrical resistance averaged 231.4 ± 5.5, which was similar to the 6th day before artificial insemination (222.5 ± 6.3). Transvaginal ultrasonography showed that most of the cows ovulated one day after artificial insemination. Conclusions: The accuracy of estrus is high if the vaginal electrical resistance is measured for cows with confirmed estrus, making is a potentially useful for determining the timing of artificial insemination.

Forward head posture (FHP) is a musculoskeletal disorder that causes neck pain. Several exercise interventions have been used in South Korea to improve craniovertebral angle (CVA) and relieve neck pain. There has been no domestic literature review study over the past 5 years that has investigated trends and effects of exercise intervention methods for CVA with neck pain. This domestic literature review aimed to evaluate the trends and effects of exercise interventions on CVA and neck pain in persons with FHP. A review of domestic literature published in Korean or English language between 2018 and 2022 was performed. Literature search was conducted on Google Scholar and Korea Citation Index by using the following keywords: “exercise,” “exercise therapy,” “exercise program,” “forward head posture,” and “neck pain.” Ten studies were included in this review. All of the studies showed positive improvements after intervention programs that included exercises. Notably, four of these studies demonstrated significant differences in results between the experimental and control groups. Among the 10 studies, nine measured visual analogue scale or numerical rating scale scores and reported significant reductions in pain following interventions, including exercise programs. Five of these studies showed significant differences in results between the experimental and control groups. Furthermore, six studies that used neck disability index exhibited a significant decrease in symptoms after implementing intervention programs that included exercise, and significant differences in results were found between the experimental and control groups. This domestic literature review provides consistent evidence to support the application of various exercise intervention programs to improve CVA and relieve neck pain from FHP. Further studies are warranted to review the effects of various exercise interventions on FHP reported not only in domestic but also in international literature.

Fungal contaminant in the indoor air is one of risk factors that could damage valuable paper-based records preserved in libraries. In the process of monitoring airborne fungi at the Nara Repository, the National Archives, Seoul, Korea, three fungal strains, DUCC 16098, DUCC 17764, and DUCC17767 were isolated from the archive’s air samples. Fungal identification was performed based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS), the 28S LSU rDNA, and β-tubulin gene (BenA), and TEF1-α gene. These DUCC 16098, DUCC 17764 and DUCC17767 strains were identified as Clonostachys farinosa, Penicillium cosmopolitanum, and Cephalotrichum purpureofuscum. These species have not been recorded before in Korea. Information on these fungi will help the monitoring and management of airborne fungi in the archival facilities.

Background: Water deer and sika deer, which breed in the wild environment, are known to have similar reproductive physiology mechanisms. Therefore, this study aimed to analyze the differences in uterine development between water deer and sika deer during estrus. Methods: MMPs and uterine development-related factors were analyzed and morphological differences were compared in the uterus of sika deer captured near Russia near Korea and water deer captured in the wild in Korea. Results: In terms of morphological differences in the uterus, the glands that form villus within the endometrium of the water deer were newly developed, and the formation of small glands was high, but the villus and glands of the sika deer were expanded, and the stroma zone in the myometrium was higher than that of the water deer. Development has increased. Additionally, the expression of PAPP-A and VEGF factors was increased in the endometrium of water deer than in sika deer, but the actions of MMPs were increased in sika deer. Conclusions: As a result of this study, there is a significant difference in the development of glands in the endometrium of water deer and sika deer during estrus, and it is believed that there is a significant difference in the development of the uterus due to the physiological effects of estrus between water deer and sika deer. Additionally, it is believed that there will be differences in the timing at which pregnancy can be decided.

Air conditioner filters purify the air of indoor environments by removing air pollutants and supporting the efficiency of the unit’s cooling function. However, an air conditioner filter can become a microenvironment in which some fungi can grow as dust continues to accumulate and favorable humidity conditions are formed. Fungal growth in air conditioner filters could lead to fungal allergies or fungal diseases, in addition to emitting a foul odor. In an effort to understand what species causes this malodorous problem, we investigated the diversity of fungi found in air conditioners. Fungi were sampled from the collected air conditioner filters and grown on DG18 agar media. After purification for pure isolates, species identification was undertaken. Colony morphology was observed on PDA, MEA, CYA, and OA media. Microstructures of fruiting body, mycelia, and spores were examined using a light microscope. Molecular identification was performed by PCR and sequencing of PCR amplicons, and molecular phylogenetic analysis of sequenced DNA markers, including the Internal Transcribed Spacer (ITS), the 28S large subunit of the nuclear ribosomal RNA (LSU rDNA), the β-tubulin (BenA) gene, the Calmodulin (CaM) gene, and the DNA-directed RNA polymerase II subunit 2 (RPB2) gene. Through this identification process, we found two fungal species, Aspergillus miraensis and Dichotomopilus ramosissimus, which are unrecorded species in Korea. We will now report their morphological and molecular features.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

In this study, quantitative analysis is attempted on data collected from Chilgapsan Observatory Star Park in Cheongyang-gun, Chungcheongnam-do. The aim of this experimental study in which quantitative analysis of the Astronomical Science Museum in Korea is conducted is to investigate its current situation and secure basic data. As of July 31, 2023, it has had 283,931 cumulative visitors in total. It had the largest number of visitors when it opened (2009 year), after which their number reduced steadily until the pandemic (COVID-19, 2020–2022). Recently, however, the number of visitors has increased. Generally, the number of visitors is highest in August (20.8$\%$) and least in April (4.1$\%$). The visit rate is higher on weekends (Saturday and Sunday) than on weekdays (Monday–Friday), and groups comprise only about 5.3$\%$ of the total number of visitors. Moreover, it can be confirmed that the number of visitors increases sharply during events. Finally, it was confirmed that the visit rate was unaffected by weather conditions. Considering these results, we propose the following strategies: 1) Establish a special program that reflects “the weekend effect.” 2) Prepare a plan to attract group visitors during the weekdays using “the event effect.” 3) Arrange alternative programs (e.g., experiential activities) that can be conducted indoors regardless of weather conditions. We think that our findings will help establish a roadmap for the direction the Astronomical Science Museum should take and aid in preparing a strategic foundation to preemptively respond to unexpected situations (e.g., pandemics).

최근 다양한 생성형 인공지능 프로그램 기술이 발전하며, 대중적으로 상용화되면서 생성형 인공지능을 활용 한 미디어아트의 창작사례가 늘어나고 있다. 사진의 등장이 기존의 회화 작품을 대체하지 않고 새로운 예술 형태로 존재한 것과 같이 생성형 인공지능 기술을 활용한 미디어아트 예술 표현 또한 새로운 방식과 형태로 존재할 수 있다. 논문에서 열거한 생성형 인공지능 프로그램을 활용하여 창작된 사례들과 같이 하나의 작품 으로 증명이 되고 있다. 이에 본 논문에서는 인공지능의 의미와 역사적으로 형성된 생성형 인공지능을 활용 한 작품 사례들을 살펴보고, 다양한 형태로 존재하고 있는 생성형 인공지능 프로그램을 기반으로 형성된 미 디어아트 작품을 분석해보고자 한다. 이를 통하여 방대한 빅데이터를 기반으로 존재하고 있는 생성형 인공지 능의 기술을 자유롭게 창작자가 예술작품으로 사용하며 다채로운 형태로 전시된 미디어아트의 제작과 방법 론에 보탬이 되고자 한다.

본 사례 연구는 실시간 음성인식을 결합한 설치 작품의 기술, 언어학과 인터랙티브 아트의 융합을 탐구하는 것을 목표로 한다. 따라서 음성인식 기술이 언어라는 축을 통해 ‘Spect’actor’에게 어떻게 전달할 수 있는지에 대한 예술적 관점과 심도 깊은 이해를 도모하고자 한다. 본 연구는 음성인식 기술을 통한 미디어아트 해석 의 가능성을 전반으로 확대하고, 특히 인터랙티브 미디어아트 분야에서 회화적 자동 음성인식을 통한 미디어 아트 해석의 가능성을 넓히고자 합니다.

Throughfall (TF)—as a diffusive hydrological water flux—significantly affects ecohydrological and biogeochemical processes within forest ecosystems. Recent investigations have revealed the impact on TF generation processes within unmanaged coniferous plantations of under-canopy structures, particularly those laden with dead branches, as well as upper-canopy structures. However, spatiotemporal variations in TF in such plantations remain unexplored. We investigated these variations in TF in a 33-year-old unmanaged Japanese cypress (Chamaecyparis obtusa Endl.) plantation, laden with dead branches, with a high stand density (SD) of 2,500 stems ha−1. Over a two-year period (May 2017 to May 2019), we conducted weekly TF measurements using 28 manual-type TF collectors. We compared the present TF ratio and canopy water storage capacity (S) with those of previous investigations conducted on Japanese cypress plantations. Moreover, we assessed key indices contributing to spatiotemporal TF variations (canopy cover: CC and distance to the nearest stem: TFd) and potentially influential dead branch indices (number of dead branches: TFdb and vertical spacing length on a stem: TFs) to elucidate TF spatial patterns. The results showed that the TF ratio was notably lower than that in previous studies (n = 13), with SD (r = –0.92, p < 0.001) and S (r = –0.87, p < 0.001) emerging as key influential factors among other stand-structure parameters. Spatial TF patterns exhibited a decreasing trend as the gross rainfall (GR) increased. Temporal stability was not significantly associated with CC (r = 0.120, p = 0.544), TFd (r = 0.068, p = 0.731), TFdb (r = 0.211, p = 0.281), or TFs (r = 0.206, p = 0.292) for any of the TF collectors. These findings underscore the important role of GR in determining the spatial variation of TF. Collectively, our results contribute to an enhanced understanding of TF spatiotemporal heterogeneity in unmanaged Japanese cypress plantations with dead branches.

In this review, the regulatory mechanisms of autophagy were described, and its interaction with apoptosis was identified. The role of autophagy in embryogenesis, tooth development, and cell differentiation were also investigated. Autophagy is regulated by various autophagy-related genes and those related to stress response. Highly active autophagy occurrences have been reported during cell differentiation before implantation after fertilization. Autophagy is involved in energy generation and supplies nutrients during early birth, essential to compensate for their deficient supply from the placenta. The contribution of autophagy during tooth development, such as the shape of the crown and root formation, ivory, and homeostasis in cells, was also observed. Genes control autophagy, and studying the role of autophagy in cell differentiation and development was useful for understanding human aging, illness, and health. In the future, the role of specific mechanisms in the development and differentiation of autophagy may increase the understanding of the pathological mechanisms of disease and development processes and is expected to reduce the treatment of various diseases by modulating the autophagic phenomenon.

최근 기술이 발전함에 따라 다양한VR (Virtual Reality) 및AR (Augmented Reality) 장치들이 등장하고 있다. 이 러한 장치들의 대부분의 콘텐츠는 사용자 상호작용을 위해 컨트롤러에 의존하고 있으나 컨트롤러는 휴대하 기 어렵고 분실하기 쉽다는 단점이 있다. 또한, 컨트롤러를 통한 사용자 인터페이스는 장치에 익숙하지 않은 사용자들이 불편함을 느낄 수 있고 새로 인터페이스를 배워야 하는 한계가 있다. 이러한 문제를 해결하기 위 해Oculus Quest는 핸드 트래킹 기술을 도입하여 사용자가 자연스러운 손 제스처를 인터페이스로 사용할 수 있게 하였다. 그러나 핸드 트래킹을 활용하는 방법은 그 어려움 때문에 아직 널리 연구되지 않았고 아직 활 용되지 못하고 있다. 이에 VR 콘텐츠와 상호작용하는 데 손을 사용하는 방법을 탐구하고, 그것을 이용하여 콘텐츠를 만드는 방법을 설명한다

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.