A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

(E,E)-8,10-dodecadienyl acetate (E8E10-12:Ac) and (E)-8-dodecaenyl acetate (E8-12:Ac) have been selected as the candidate chemicals for sex pheromone components of the M. phaseoli, female through GC-EAD tests, whereas the two compounds and an additional candidate, (E,Z)-7,9-dodecadienyl acetate (E7Z9-12:Ac), have been found at a ratio of 7:1:1 in the abdominal tip extract (Yum et al., 2008). In order to determine the actual composition of sex pheromone, therefore, several blends using the three chemicals were evaluated for attractiveness to males of M. phaseoli around red bean and soybean fields. Individual components as well as two blends consisted of E8E10-12:Ac/E7Z9-12:Ac and E8-12:Ac/E7Z9-12:Ac did not show attractiveness, whereas the blend of E8E10-12:Ac/E8-12:Ac showed an increased effect in male capture. Of the tested blends with all three chemicals, the 7:1:2 composition of E8E10-12:Ac, E8-12:Ac and E7Z9-12:Ac attracted the most number of males. The results suggested that E7Z9-12:Ac is one of the sex pheromone components and may act as a synergist.

As indigenous aphid parasitoid, Aphelinus varipes kill aphids for feeding in addition to parasitization. Because of this characteristic of A. varipes, this parasitoid may have the possibility of biological control agent against aphids. So we have evaluated traits such as daily paratization, total parasization, number of aphids killed by host feeding, sex ratio, development time, pupal mortality of A. varipes parasitizing green peach aphid, Myzus persicae. At 25°C and 16L:8D, longevity, total paratization and host feeding of A. varipes female was 11.0, 25.3, and 63.3 days, respectively. And development time of male and female, sex ratio (M:F), pupa mortality of offspring of A. varipes were 12.0 days, 12.5 days, 0.88, and 11.6%, respectively. However, because these results are not enough to estimate potential of A. varipes as biological control agents/factors, other factors such as host suitability (Macrosiphum euphorbiae, Aulacorthum solani), effect of temperature, and host seeking behavior of A. varipes continually will be investigated.

As an effective generalist predator of aphids and other hemipteran pests, Harmonia axyridis has been a successful biological control agent. Interestingly, it was known that there were varied in color patterns on H. axyridis elytra. In fact, Seo & Youn (2007) reported that H. axyridis had five color patterns, for example, succinea 1, 2, conspicua, spectabilis, and axyridis. But there are uncertain that H. axyridis elytra colour patterns are regulated by genetic polymorphism. So we tried to what is the reason that color patterns are greatly variable. To identify DNA markers linked to a elytra polymorphism, amplified fragment length polymorphism (AFLP) analysis was performed on DNA samples from four female succinea, conspicua, spectabilis and Coccinella septempunctata which is another species in Coccinellidae. AFLP analysis with the restriction endonuclease combination EcoRⅠ and MseⅠwas performed. Using 12 AFLP primer pairs, nine AFLP fragments which is specific between succinea, conspicua, spectabilis was identified. These nine AFLP fragments were isolated, cloned and sequenced. Subsequent UPGMA cluster analysis revealed three major group of H. axyridis populations. These genetic tree showed that H. axyridis elytra colour diversity was affected by genetic polymorphism. For more genetically understanding elytra colour genes, different primer combinations may be need to be generate enough polymorphic markers. These genetic analyses may be facilitate the understanding of molecular mechanism behind wing colour pattern formation.

This study was carried out to investigate the effect of each single component of the synthetic sex pheromone in the rice green caterpillar, Naranga aenescens and rice leaf roller, Cnaphalocrocis medinalis. Trap catches with each single component or binary mixtures of the sex pheromone in N. aenescens were significantly lower than that with an optimum mixture (2: 1: 4) of Z9-14:Ac, Z9-16:Ac and Z11-16:Ac. But only few number of C. medinalis male was caught in trap baited with each component of the sex pheromone excepting Z13-18:Ald. In an optimum composition of the sex pheromone in C. medinalis, trap catches baited with an 11: 100: 11 mixture of Z11-18:Ald, Z13-18:Ald, and Z13-18:Ac without two alcohol components (Z11-18:OH and Z13-18:OH) was higher than that baited with an optimum mixture of Z11-18:Ald, Z13-18:Ald, Z11-18:OH, Z13-18:OH, and Z13-18:Ac. The efficacy of the sex pheromone lure of N. aenescens kept over 4 weeks. The occurrence of N. aenescens caught in trap baited with sex pheromone gave three peaks and C. medinalis showed two distinct peaks throughout the flight season. Therefore, it can be expected that pheromone-baited trap provides a simple tool for monitoring N. aenescens and C. medinalis in Korea.

This study was carried out to identify the sex pheromone of the soybean podworm, Matsumuraeses phaseoli. EAG response of M. phaseoli male antenna to various chemical compounds were examined. Of them, (E,E)-8,10-dodecadienyl acetate (E8E10-12:Ac) and (E)-8-dodecenyl acetate (E8-12:Ac) were most EAG-active. The abdominal tips of M. phaseoli females were extracted with distilled hexane 4h after light-off for 30 min. In an electroantennographic detection (GC-EAD), female extracts showed two EAG-active components. In a GC-MS analysis, three components (E8E10-12:Ac, E8-12:Ac, and (E,Z)-7,9-dodecadienyl acetate) were identical with those of authentic standards following the retention time and their ratio was 7: 1: 1. Of these three components, EAG responses of M. phaseoli males to E8E10-12:Ac and E8-12:Ac were significantly dose-dependent. Field effectiveness of these components remains to be evaluated.

Somatic cell nuclear transfer in cattle has limited efficiency in terms of production of live offspring due to high incidence of fetal failure after embryo transfer to recipients. Such low efficiency of cloning could possibly arise from abnormal and poorly developed placenta. In the present study the placental proteome in late pregnancy established from in vitro fertilization (IVF) and nuclear transfer (NT) was analysed. Proteome alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing placenta from NT embryos to those from IVF counterparts, significant changes in expression level were found in 18 proteins. Of these proteins 12 were not expressed in NT placenta but expressed in IVF counterpart, whereas the expression of the other 6 proteins was limited only in NT placenta. Among these proteins, cytokeratin 8 and vimentin are considered to be involved in regulation of post-implantation development. In particular, cytokeratin 8 and vimentin may be used as makers for placental development during pregnancy because their expression levels changed considerably in NT placental tissue compared with its IVF counterpart. Data from 2-DE suggest that protein expression was disorientated in late pregnancy from NT, but this distortion was eliminated with progression of pregnancy. These findings demonstrate abnormal placental development during late pregnancy from NT and suggest that alterations of specific placental protein expression may be involved in abnormal function of placenta.

해양구조물의 피로파괴 형태는매우다양하며 설계단계에서 구조요소의 피로수명 예측은중요하다. 각가지 피로해석 방법에 대해서 연구가 활발히 진행되었으며 접근방법에 대한 논의도 활발한 연구와 함께 진행되었 다. 본 논문에서는 피로해석 방법중 스펙트랄 방법과 그 구성요소에 대해서 연구되었으며 간략화된 피로해석 방법이 제시되었고 그 특성이 비교 검토되었다. 두가지 피로해석 방법의 장단점이 조사되었고 관련된 인자인 웅력집중계수， 웅력폭-수명 관계 곡선 또한 연구되었다. 전형적인 반 잠수식시추선의 브레이싱 부재의 피로수명 예측을 위하여 간략화된 피로해석 방법과 스팩트랄 피로해석 방법이 작용되었으며 이롤 통하여 두 방법의 유용성이 확인되었다. 또한 간략화된 피로해석 방법을 이용한 민감도 해석이 수행되었다. 본 논문에서 수행된 피로해석 결과는 스펙트랄 피로해석 방법이 보다 현실 적인 피로수명 예측을 할 수 있는 방법이라는 사실올 보여주었다.

We have conducted BVI photographic surface photometry of four spiral galaxies NGC1087, NGC2715, NGC2844 and NGC3593, by making use of the Kiso Schmidt plates. Detailed examination of the morphological properties of the galaxies using isophotal maps and luminosity profiles showed that all the program galaxies have some peculiarities in their luminosity distributions. NGC1087 and NGC2715 have extremely small nuclei with inner rings which contain several bright HII regions. NGC2844 has a very large bulge whose luminosity dominates over the disk luminosity in all the radii. The I-band luminosity profile of NGC3593 shows shallower gradient than B- and V-band profiles. We were able to successfully decompose the luminosity profile into a bulge following de Vaucouleurs γ 1 / 4 − l a w and an exponential disk only for NGC 3953. Other galaxies have more complicated luminosit profiles.

파이 프 설 t서는 다양한 두께 및 직경， 길이 룹 가진 파이프뜰로 이 루 어진 ':rL조 늄로 서 정확한 해석 을 위해서논 3차원의 유한 요소 모델이 필요 하다 그러나 유한효소 모덴에 의한 헤석 l상빈은 계산 시 간이 길어 칠 뿐만 아니라 방대한 양의 컴퓨터 용량을 핀요로 한다. 반변에， 파이 프 륜 보 요소로 가 정한 단순 한 해석 모덴은 파이프 설비의 정확한 서동을 예측하기에 문제점이 많 다. 따라서， 본 연구에서는 파이프 설비의 효율적인 해석 모탤을 제안하고자 한다. 제안된 해석 모델 은 보요소로 구성된 모델에 접합부 요소만을 추가함으로써 보요소만으 로 구성된 모델만큼 단순하며， 또 한 파이프 접합부의 국 부적 인 변형을 고려하기 때 문 에 파이프 설비의 거동 을 정확히 예 꽉 할 수 있다. 예제 해석 올 통하여 결과들을 비교함으로써 본 연구에서 제안한 모델의 효융성올 입 증하였다.

직교 이방성으로 보강된 평판 구조물 해석을 위한 방법제시가 본 논문의 주요 내용이다 이 방법에서 는 변형된 정적 압축법올 이용하여 직교 이방성으로 보강된 평판 구조물과 동퉁한 강성을 가지는 2차원 그렬리지 구조물을 생성하여 해석에 응용하고 있다. 대표적인 구조물을 선택하여 이론을 적용시켜 해석 을 한 결과 직교 이방성으로 보강된 평판 구조물 해석에는 본 논문의 방법이 매우 효과적임이 입증되었 다.

In order to understand the characteristics of soil according to the cultivation environment of Chinese bellflower (Platycodon grandiflorum A.), soil chemical properties of 12 collected soil samples from 6 cultivated fields in Okcheon, Chungbuk province in August. 2017 were analyzed. The soil pH was distributed within the range of 4.61 to 5.25 at all cultivation years and E.C (Electric Conductivity) and T-N (Total Nitrogen) of the cultivation year were not significant. Available P2O5 was higher than the average for medicinal crops and P. grandiflorum in Korea and C.E.C (Cation Exchange Capacity) was inconsistent for each cultivation year. In particularly, it was validated that the content of exchangeable cations K, Ca, Ma, and Na in this experiment was similar to that of C.E.C according to the cultivation years, because C.E.C had a high correlation with the exchangeable cations. For the available P2O5, as affected by trans-planting, 5Y-NT-H (cultivated 5 years and non-transplanted) had 58 mg/kg, while 5Y-T-H (cultivated 5 years and transplanted) had 246 ㎎/㎏. The soil pH was found to be lower (acidic) in diseased soils than healthy soils. E.C was confirmed to be was higher in diseased soils than healthy soils except for the one cultivated for 2 years. The contents of T-N and available P2O5 were higher in diseased soil except for the one cultivated for 5 years and 11 years. The exchangeable cation K and Na tended to be higher in diseased soils rather than that in healthy soils, and the exchangeable cation Ca and Mg contents were higher in healthy soils than in diseased soils. The C.E.C of the soil was lower than that of healthy soils in all of the years except for the one which was cultivated for 5 years (transplanted).

본 연구에서는 다중 기후모델에 의한 미래 기후자료를 기반으로 SWAT-K 유역모형을 적용하여, 제주도 지역의 미래 기후변화에 따른 수자원 영향을 평가하였다. 기후모델에 따른 미래 전망자료의 불확실성을 고려하여 9개의 GCM 모델의 기후자료를 미래기간(2010~2099년)에 대한 SWAT-K 모형의 기상자료로 적용하였다. 과거(1992~2013년) 및 미래기간에 대한 연도별 수문변화를 분석한 결과 강수량, 유출량, 증발산량, 함양량 모두 증가하는 추세로 나타났다. 과거기간에 비해 유출량의 변화가 가장 크게 나타났으며(최대 50% 증가), 증발산량은 상대적으로 작게 나타났다(최대 11% 증가). 월별로는 8월과 9월의 강수량 증가에 따라 유출량과 함양량도 크게 증가하는 반면, 동일기간에 대한 증발산량은 감소하는 것으로 분석되었다. 1월과 12월은 반대의 경향이 나타났다. 미래의 물수지 변화를 분석한 결과 강수량 대비 유출량, 증발산량, 함양량의 비율은 변화가 크지 않으나, 과거와 비교했을 때 RCP 8.5 시나리오에서 유출량 비율은 최대 4.3% 증가하는 반면, 증발산량 비율은 최대 3.5% 감소하는 것으로 나타났다. 기존의 타 연구와 본 연구에서 도출한 결과를 종합해 볼 때, 현재 제시되고 있는 기후변화 시나리오 가정 하에서는 미래로 갈수록 점차 강수량과 유출량이 증가할 것이고 특히 여름철 강수량 및 유출량의 증가가 예상된다. 이로 인해 제주도 지역의 함양량도 함께 증가할 것으로 판단할 수 있다. 다만, 본 연구는 장기적인 측면에서 자연적인 기후변화로 인한 영향을 분석한 것이며, 추가적으로 단기적인 수재해 대응을 위한 홍수와 가뭄관리, 인위적인 용수수급 관리 등에 대한 종합적인 분석을 통해 제주도 수자원의 지속가능한 이용을 위한 대응방안이 필요하다고 판단된다.

Background: Mahonia Nepalensis DC. (Hoang lien o ro), the specie of the family Berberidaceae, is widely distributed in the high mountainous areas at altitudes 1700 – 1900 m of Vietnam. It is found that the stem of Mahonia nepalensis indicated anti-inflammatory, anti-bacterial and antifungal activities and they are used particularly for the treatment of eczema, psoriasis, and other skin conditions. However, no study on the antioxidant and anti-cancer activities of Mahonia Nepalensis stem has been previously reported. The aim of this study was to evaluate anti-oxidant and anti-cancer activities of Mahonia Nepalensis stem. Methods and Results: The stem pieces of Mahonia Nepalensis were dried and extracted three times with 100% methanol. After that, the extract was suspended in distilled water and then partitioned with n-hexane, ethyl-acetate (EtOAc) and butanol (water saturated BuOH) fractions were then evaporated using a vacuum rotary evaporator. Evaluation of the anti-oxidative activity of Mahonia Nepalensis was carried out using a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-producing system. The results revealed that the ethyl acetate fraction of M. nepalensis possessed higher potential DPPH radical scavenging activity (IC50, 81.88 ± 1.33㎍/㎖) than other fractions as well as BHT (2,6-Di-tert-Butyl-4-methylphenol) (IC50, 250.49 ± 1.60㎍/㎖). The reducing power assay was also investigated and EtOAc fraction showed higher absorbance values than other fractions. At 1.0 mg/ml concentration, EtOAc fraction showed absorbance of 1.72, be higher than Ascorbic acid. Cell viability was evaluated according to the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl tetrazolium Bromide) assay. By MTT assay, all fractions showed a significant reduction in cell viability on COLO 205 (Human colon carcinoma cell) at the highest concentration tested (200㎍/ ㎖) with over 70% decrease in cell viability was obtained, and the highest significantly inhibiting effect occurred in butanol fraction with approximately 90% reduction in cell viability. Conclusion: We demonstrated that Mahonia Nepalensis stem extract has highly potential in anti-cancer activity. Further studies are necessary in order to explore the variety of Mahonia Nepalensis stem to be applied as a valuable natural material.

Background : The use of the rain shelter facility gets more prevalent in the Ginseng cultivation area these days. This study is designed to establish a technique for the stable cultivation of Ginseng in the rain shelter facilities in high temperature (above 30 ℃) without the damage from high temperature Methods and Results : This study was carried out on 3-year Ginseng roots in 2016 in order to find out how to stably cultivate Ginseng in high temperature without suffering the high temperature damage during the cultivation of Ginseng under rain shelter facilities. The rain shading materials were coated with scattering film (scattering film + black shading net 90%), bluish white double-sided film (shading 85%) and PE film (PE film + black shading net 90%). The damage reduction by high temperature was made to the scattering film and bluish-white double-ended film only. An aluminum screen (shading rate: 40%) was installed when the high temperature (above 30℃) was reached while the isoprene (solution of 2000 times) was sprayed 4 times every 7 days from the full development stage. The light transmittance was 12.6 - 13.4% for the scattering film, 10.5 - 10.8% for the bluish-white double sided film and 7.1% for the PE film in the first coating while it was 7.3% for the scattering film and 7.1% for the bluish-white double-sided film when the aluminum screen was installed in high temperature. The high, average and low values in the relative humidity were higher inside the facility than in the outside during the survey period. The area of leaf was the largest under the scattering film. The area of leaf was the largest for the scattered film under the aluminum shade of 40% shading followed by that of spraying of isoprene 4 times, and that of the no-treatment. As for the growth under ground, the growth of underground shoots increased by 40% in aluminum screen, 53.7% in shading and 26.1% in the spraying of isoprene 4 times than non-shading while there was no difference among other rain shelter materials. Conclusion : The result of the research showed that when the scattering film is selected as the rain shelter material film for the cultivation of Ginseng, it is necessary to shade the sunlight as much as 40% by using the aluminum screen or the shading net to reduce the high temperature damage.

The goal of this study is to investigate the effectiveness of the use of multiple materials in plate-like structures structure and provide engineers and designers an appropriate view point of multi-material topology optimization when making decision and information in design. Element density distribution contours of mixing multiple material densities are linked to Solid Isotropic Material with Penalization (SIMP) as a design model. The mathematical formulation of multi-material topology optimization problem solving minimum structural compliance is an alternating active-phase algorithm with the Gauss-Seidel version as an optimization model of optimality criteria. Some numerical examples are considered to illustrate the reliability and accuracy of the present design method for multi-material topology optimization.

This paper studies about the buckling analysis of multi-material structure especially compressed column using topology optimization. The buckling is stated as a constraint in the optimization problem. A clamped-pinned column with applied axial compressive load is analyzed. An active-phase algorithm is used to solve multi-phase topology optimization problem. The distribution of different materials is determined in a isotropic two-dimensional design domain. The material properties is modified based on the Solid Isotropic Material with Penalization (SIMP) interpolation approach. The Method of Moving Asymptotes (MMA) is used to update the topology design variables which is relative element densities. The optimal designs of the column structure are presented and discused in the numerical applications.

Background : Recently, there is a urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. In order to develop ginseng varieties with such traits, systematic breeding program and comprehensive field studies are prerequisite. Methods and Results : ‘Cheonmyeong' seeds were collected in 2008 from the farmer field of Buyeo. Physiological investigation and propagation were conducted from 2009 to 2011. It was given the name 'Eumseong No. 8 through the observed yield trial from 2012 to 2013 and local adaptability was carried out from 2014 to 2015. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. Yellow-red leaf and pink berry at maturing stage were observed. The time of emergence, flowering and berry maturity of the ‘Cheonmyeong’ were faster than those of ‘Chunpoong’. Stem length of ‘Cheonmyeong’ was shorter than that of ‘Chunpoong’, whereas stem diameter was thicker than that of ‘Chunpoong’. Main root length was shorter but main root diameter is thicker than that of ‘Chunpoong’. Number of seeds of ‘Cheonmyeong’ was fewer than that of ‘Chunpoong’ but 1,000-seeds weight and stratification rate were higher than those of ‘Chunpoong’. The yield performance of this variety was 661 kg/10 a in local adaptability test for two years, which is 22% higher than that of ‘Chunpoong’. ‘Cheonmyeong’ showed strong resistance to phytophthora blight, mulberry mealybug and nematode and moderate resistance to alternaria blight. ‘Cheonmyeong’ did almost not occur yellow spot of aerial part and rusty skin of root, show moderate resistance at high temperatures. Conclusion : Our study demonstrated that ‘Cheonmyeong’ is an ideal variety with heavier root weight and enhanced stress resistance and contribute will enhance biotic/abiotic stress resistance and increase the farmers' income.