본 연구는 거리화단 화훼경관 조성 전·후의 스트레스 변화 를 알아보기 위하여, 순창 소재의 경찰관 총 18명을 대상으로 타액 채취 및 설문조사를 실시하였다. 생리 측정은 타액 코르 티솔, 혈압, 심리 측정은 식물 재배 활동 수준, 스트레스수준, 거리화단 화훼경관에 대한 간이 SD 감성을 측정하였다. 타액 코르티솔, 혈압의 사전/후 비교는 대응표본 t-test, 타액 코르 티솔, 혈압의 성별에 따른 집단간 사전/후 비교는, 독립표본 t-test, 스트레스수준, 간이 SD는 윌콕슨 부호 순위 검정 등으 로 분석하였다. 화훼경관 조성 전보다 조성 후 총 코르티솔 수 치가 낮아졌고, 식물 재배 활동 수준은 높아졌으나 통계적 유 의성은 없었다. 혈압은 여자의 경우 거리화단 화훼경관 조성 전과 조성 후 모두 정상 혈압이었고, 남자의 경우 거리화단 화 훼경관 조성 전 고혈압 전단계에서 조성 후 주의혈압으로 낮 아지는 긍정적 효과가 있었다. 거리화단 화훼경관 조성 전·후 의 스트레스수준을 총 4단계(총 4단계: 단계가 높을수록 스트 레스가 높은 수준임)로 문항 전체의 총합 수치로 알아본 결과, 화훼경관 조성 전에는 4단계(50.0%), 2단계(38.9%), 3단계 (11.1%) 순이었으나, 조성 후에는 4단계(44.5%), 2, 3단계(각 22.2%), 1단계(11.1%) 순으로 거리화단 화훼경관 조성 전에 는 1단계(정상적인 스트레스 상태로 스트레스요인 자체가 심 각하지 않거나 좋은 스트레스로 받아들인 경우)가 0%에서 조 성 후 11.1%로 높아졌다. 화훼경관 조성 전·후 SD법을 이용 하여 쾌적감, 자연감, 그리고 진정감에 대해 평가한 결과, 화 훼경관 조성 후 쾌적감, 자연감, 진정감이 높은 것으로 나타났 다(p<.05). 거리화단 화훼경관에 따른 식물 재배 활동 수준과 주요 변수간의 상관관계를 알아본 결과, 화훼경관 조성 후 식 물 재배 활동 수준과 스트레스수준, 스트레스수준과 진정감은 부(-)의 상관관계, 식물 재배 활동 수준과 쾌적감, 자연감, 진정 감과는 0.5 이상의 높은 정(+)의 상관관계가 있었다(p<.05). 따라서 식물 재배 활동 수준이 높아질수록 쾌적감, 자연감, 그 리고, 진정감은 높아지고, 거리화단 화훼경관이 없는 공간보 다 화훼경관이 있는 공간에서 쾌적감, 자연감, 그리고 진정감 을 더 크게 느끼는 것으로 판단되었다. 본 연구에서 거리화단 화훼경관을 통하여 고위험군의 높은 스트레스는 줄여주고 식 물에 대한 흥미, 관심과 심리적 안정효과는 높여 줄 수 있는 유용한 가능성이 있을 것으로 생각되었다.

장수풍뎅이는 우리나라 곤충 산업에서 학습𐤟애완곤충으로 활용도가 매우 높으며 전국적으로 많은 농가에서 사육 되고 있다. 최근 식용, 약용으로도 허가가 되어 농가의 수가 확대되고 있고, 대부분 번식 개체 암수 비율 증가(2:1 이상), 대형 상자, 대량의 발효톱밥을 이용하여 번식을 한다. 이는 몇몇 농가들에게는 경제적 비용을 늘리는 요인이 되어 왔다. 본 연구에서는 비용 절감 방안을 모색하고자, 칸막이 사육 상자를 고안하여 한 쌍으로만 실내 번식(온도 25℃, 습도 60%)을 실시하였다. 조사 결과, 칸막이가 없는 사육 상자에서 평균 산란수는 93.6개였고, 칸막이가 있는 처리구의 경우에는 평균 산란수가 121.3개로 칸막이가 없는 것보다 알을 더 많이 낳는 경향을 보였다. 또한, 발효톱밥의 경도가 높은 처리구에서 산란을 더 많이 하는 경향을 보였다.

일반 시설재배지는 겨울을 포함한 저온 시기에 외부의 온도 변화에 영향을 가능한 적게 받기 위한 형태였다면, 최근에는 환경제어기술이 발달하면서 온도, 습도, CO₂, 광량 등을 제어할 수 있는‘스마트팜(Smart-farm)’이 농가에 보급되고 있다. 한편, 토마토 시설재배지에서 많이 발생하는 대표적인 미소해충인 담배가루이와 작은뿌리파리는 유묘나 창문과 출입문의 개폐과정 중에 유입되어 피해를 입히는 것으로 알려져 있다. 스마트팜 내의 해충 발생밀도는 시설재배지 내부의 미소환경에 따라 달라질 수 있다. 따라서 이러한 미소환경의 패턴을 조사하면 좀 더 정확한 해충 발생예찰이 가능할 것으로 생각된다. 본 연구에서는 서로 다른 지역에 위치한 4개의 스마트팜에서 온도와 습도, 그리고 담배가루이와 작은뿌리파리의 해충밀도를 황색 끈끈이 트랩을 이용하여 1주일 간격으로 조사하여 스마트팜 내의 환경변화에 따른 담배가루이와 작은뿌리파리의 수평적, 수직적 밀도분포를 분석하였다.

토마토는 어느 정도 높은 온습도에서 잘 자라기 때문에 주로 비닐하우스에서 재배되어왔다. 최근에는 정보통신기술 (ICT)의 발달로 온도, 습도, CO₂등의 환경요소를 원격으로 조절하여 힘과 시간을 덜 소모하고도 작물의 생육환경을 편리하게 관리할 수 있는 스마트팜(Smart farm)이 늘어나고 있다. 본 연구에서는 토마토 비닐하우스 및 스마트팜에서의 위치별 온․습도와 토마토의 주요 해충인 담배가루이와 작은뿌리파리의 발생량 및 시설 내 발생분포를 조사하였다. 조사결과, 시설재배지 내의 위치에 따라 온․습도 및 해충 발생량에 차이가 나타났으며, 작은뿌리파리의 경우 시설의 가장자리 및 낮은 위치에서, 담배가루이는 시설의 중앙부 및 높은 위치에서 밀도가 높게 나타나는 경향을 보였다.

Harmonia axyridis have become extremely popular through their extensive elytra and spot variation for many years. However, phenotypic polymorphism and striking visual phenotypes are not yet to be extensively studied. With growing attention to polymorphism of H. axyridis, it is imperative to study involved in genomic research in order to elucidate the molecular mechanisms underlying the diversity of elytra variation. To figure out suitable approach for analyzing genes of H. axyidis, we assessed RNAi as a tool for functional analysis. In order to demonstrate the utility of RNAi technique on H. axyridis, we conducted cDNA library screening to affirm silencing effect. We choose random genes from cDNA clones and the random genes were synthesized into dsRNA. After the injection of dsRNA into last instar larvae, we observed morphological defects or deaths. To investigate the effectiveness of knockdown in H. axyridis, we performed quantitative real time PCR on H. axyridis that appears the most distinctive features. These results provide that RNAi is highly applicable in H. axyridis and it will be useful for the gene functional analysis.

Insect growth regulators (IGRs) are insecticides that disrupt the normal development of target insects. Among the IGR insecticides, juvenile hormone (JH)-related IGRs are of particular interest because they stimulate or interfere with the formation of JH receptor complex. In the precious studies, novel JH-related IGRs with JH agonist (JHA) and antagonist (JHAN) activity were identified by using yeast two-hybrid system transformed with the Aedes aegypti JH receptor complex. In this study, the transcriptomic responses of Aedes albopictus were investigated upon JHA and JHAN, respectively. These results will provide important information about understanding of impact of JH-related IGRs in transcription level.

Bacillus thuringiensis (Bt) is a gram-positive bacterium that produces parasporal crystal proteins known as endotoxins or Cry proteins. The Cry protoxins are then cleaved by insect midgut proteinases to form active Bt toxins. The activated Cry protein then binds to specific receptors at the midgut epithelium. Cadherin-like and aminopeptidase N (APN) proteins are involved in Bt toxin binding by interacting sequentially with different toxin structures. Aminopeptidase N (APNs) from several insect species have been shown to be putative receptors for these toxins. We have characterized four different midgut APNs(APN1, APN2, APN3, APN4) cDNAs from S. exigua. Forward primers and reverse primers for confirmation of four different midgut APNs were designed based on their sequences cloned from the cDNA libraries. Quantitative RT-PCR procedures includes 42℃ for 20min (cDNA synthesis), 99℃ for 5min, and 35 cycles (94℃ for 1min, and 60℃ for 50 s) for collection. Four aminopeptidase N isoforms were confirmed with qRT-PCR. Sequence analysis was performed by BlastX search the National Center for Biotechnology Information(NCBI) nucleotide. Furthermore, double-stranded RNAs(dsRNAs) were synthesized. DsRNAs were determined for bioassay.

토마토 TYLCV는 병이 발생되면 약제나 다른 방법으로 방제가 어려우므로 내병성 품종의 개발이 반드시 필요하다. 특히, TYLCV 저항성 유전자 Ty-1,2,3(부분우성)을 한 개체 내로 집적을 시켜서 저항성이 증대된 품종의 개발이 필요하다. 토마토 TYLCV 저항성 11-TY1-AV 등 7계통을 대상으로 보독인 담배가루이를 이용하여 표현형을 조사하였다. 이병성 대비(11-TY5-AV; 슈퍼썬로드)와 F3 세대 3계통 및 F2 세대 4계통을 TYLCTHV 균주를 이용하여 접종하였다. 접종 13일 후에 이병성 대비가 100% 이병증상을 나타내었으며, 이때 11-TY4-AV는 48개체 중에서 23개체가 이병되었으며, 11-TY6-AV는 42개체 중에서 14개체, 11-TY7-AV는 48개체 중에서 14개체, 11-TY8-AV는 48개체 중에서 15개체가 이병 되었다. F2 세대 4 집단 모두 p>0.05 수준에서 TYLCV 접종 후 표현형이 정상적인 유전분리비(3:1)를 보이는 것을 확인 하였다. 또, TYLCV에 저항성인 동일한 자원을 부계로 사용하더라도 모계로 어떤 것을 사용 하느냐에 따라서 저항성 정도가 다르게 나타나는 것을 확인할 수 있었다.

본 연구에서는 용액 캐스팅법을 이용하여 각기 다른 함량의 VMT가 첨가된 SPAES/VMT 복합막이 제조되었다. SPAES 매트릭스 내의 VMT입자 분산은 전자주사현미경으로 관찰된 평균분포에 의해 확인되었다. 1 wt%보다 적은 함량을 포함한 복합막은 고분자 매트릭스 내에 좋은 분산성을 나타내어 막의 상부층과 하부층에 매끈한 표면을 가졌다. 복합막의 함수율은 온도가 증가함에 따라 급격하게 증가되었으며 VMT는 높은 이온교환능력으로 인하여 강한 수분친화도를 가짐으로 인해 높은 모든 흡착된 수분은 bound water인 것으로 확인하였다. VMT의 함량이 1 wt%보다 적게 첨가된 복합막에서 증가된 이온전도도와 낮아진 메탄올 투과도를 확인할 수 있었다. 모든 복합막 중에서 SPAES/VMT 1.0 wt% 복합막은 선택도 측면에서 가장 뛰어난 연료전지 성능을 가졌으며 Nafion 112과 비교하여 두배 이상 우수한 값을 나타내었다. 이것은 SPAES/VMT 1.0 wt% 복합막이 직접메탄올 연료전지의 구동을 위한 가장 우수한 조건이 될것이라 사료된다.

Nicotine, a major teratogen of cigarettes smoke induces embryonic abnormalities during the early stages of organogenesis. In this study, the protective effect of β-carotene against nicotine–induced embryos was evaluated by morphologic scoring, nile blue staining, lipid peroxidation, SOD activity assay and real-time PCR. The embryos exposed to nicotine (1 μM) revealed remarkable morphological anomalies compared to normal control group (p<0.05), but when β-carotene (1×10‒4 μM or 5×10‒4 μM) was added concurrently to the embryos exposed to nicotine, morphological parameters were significantly improved (p<0.05). Nicotine induced oxidative stress by increased lipid peroxidation, expression of proinflammatory cytokines (TNF-α and IL-1β), caspases-3 and decreased SOD activity. However, administration of β-carotene (1×10‒4 μM or 5×10‒4 μM) restored the SOD level and decreased oxidative damage in the embryos. These results indicate that β-carotene effectively counteracts the deleterious effects of nicotine on embryos and attenuates oxidative damage possibly through its antioxidant effects.

Nicotine, a major toxic component in tobacco smoke, leads to severe embryonic damages on organogenesis. We investigated if resveratrol can inhibit the nicotine–induced teratogenesis in the cultured mouse embryos (embryonic day 8.5) for 48 hours using a whole embryo culture system. The embryos exposed to nicotine (1 μM) revealed severe morphological anomalies, the increased levels of caspase-3 mRNA and lipid peroxidation, and further the lowered levels of mitochondrial manganese superoxide dismutase (SOD), cytosolic glutathione peroxidase (GPx), phospholipid hydroperoxide GPx, hypoxia-inducible factor 1α, and sirtuin mRNAs and SOD activity significantly compared to normal control group (p<0.05). However, whenre sveratrol(1×10‒5 μMor1 ×10‒4 μM) was added concurrently to the embryos exposed to nicotine, these all parameters were significantly improved (p<0.05).These findings indicate that resveratrol has a protective effect against nicotine-induced teratogenesis in mouse embryos throughout antioxidative and anti-apoptotic activities.

Previously, we found that expression by translational fusion of the polyhedrin (Polh)-green fluorescence protein (GFP) led to the formation of granular structures and these fluorescent granules were easily precipitated by high-speed centrifugation. Here, we developed an easy, fast, and mass purification system using this baculovirus expression system (BES). An enhanced GFP (EGFP) fused with Polh gene at the N-terminus including an adaptor and enterokinase (EK) site between Polh and EGFP was expressed in Sf9 cells. The cells infected by AcPolhEKA-EGFP produced fluorescent granules. The EGFP fusion protein was purified from granule-containing cells according to three steps; cell harvest, sonication and EK digestion. Through the final enterokinase digestion, EGFP was presented mainly in the supernatant (93.3%) and the supernatant also showed a pure EGFP band. These results suggest that the combined procedure of Polh fusion expression and enterokinase digestion can used for the rapid and easy purification of other proteins.

식물휘발성 물질에 대한 벼멸구 촉각의 반응을 전기 생리학적인 반응을 관찰하였다. 식물휘발성 물질은 일반적으로 곤충이 그들의 먹이나 산란 장소를 찾는데 아주 중요한 요소로 작용하고 있다. 이러한 기주 특이적인 성질을 파악하기 위하여 벼멸구 촉각에 분포하고 있는 화학감각기의 반응을 AC반응을 통하여 기록하였다. 벼멸구의 plaque organ에서 기된 spike 의 모양은 positive-going biphasic형으로 background spike는 초당 1~22개로 다양하였다. 벼멸구 촉각은 실험한 화학물질들에 광범위하게 반응하고 있는 것을 보여주었으며 농도가 높아짐에 따라 더욱 흥분되지만 일정 수준이상의 농도에서는 더 이상 흥분되지 않거나 억제되는 것을 보여주었다. 본 실험에서는 검정된 대부분의 식물 휘발성물질에서 가장 큰 반응을 보인 농도는 단위 용기 안에 100의 물질이 있을 때였고, 가장 큰 반응을 보인 화합물은 hexanal 과 acetophenone이었다.

벼멸구의 식이행동을 전기적인 측정 방법을 통하여 관찰하였으며 이들을 각각의 특성에 따라 분류하였고, 그결과 type P, S, SB, O, X, Ph 등 6가지로 나누어 볼 수 있었다. Type P는 벼멸구가 식물에체 처음 접근하여 기주를 탐색할 때 볼 수 있었으며, 구침을 조직내에 찔어 넣거나조직내에서 이동할 때에는 type S를 관찰 할 수 있었다. 또한 매우 규칙적인 S패턴은 체관부에 구침을 찔어 넣을때만 볼 수 있어 type SB로 따로이 분류하였다. X와 Ph패턴은 각각 물관부와 채관부에서 흡즙할 때 나타나는 것을 확인하였으며, type O는 기타 다른 조직내에 구침이 있을 때 이러한 전기적 패턴을 보여 주었다. 이러한 각각의 전기적 패턴을 확인하기 위하여 원하는 패턴이 나타날 때에 식흔이 있는 벼의 조직을 잘라서 현미경으로 관찰하였으며, 각각의 타입별로 벼멸구 배설물을 측정하였다.

Background : This study was conducted to develop sustainable and safe ginseng cultivation facilities to cope with climate change and to save labor due to the decrease of labor force in rural areas. Methods and Results : In 2017, we designed the wide-shading facility which was unmanned and labor saving to cope with climate warming and decreasing labor force in farming area for stable and sustainable production of ginseng. The developed model is called model Ⅰ(Slope-shading facility, Gyeongsangbuk-do ARES), model Ⅱ (Slope-shading facility, Gyeonggi-do ARES) and Model Ⅲ (Roof-type shading facility, RDA, NIHHS, Department of Herbal Crop Research). The tested varieties were stem-violet variant, and the transplanting was done by using the 2-years-old seedlings on April 3, 2017 and the planting density was 90 plant/1.62㎡. A thermometer, a light meter, and a rain gauge were installed in the center of the facility on April 13, 2017 to measure the micro-weather in the facility. Micro-weather observations were made from April 14 to October 10, measuring temperature, humidity, light intensity, soil moisture content, water leakage, and wind speed. Surveys were carried out on June 20 and September 29, respectively. The maximum temperature in the facility was 2 to 3℃ lower than the outside, but the relative humidity was 2 - 3% higher in the facility than in the outside. Soil moisture did not show a clear trend among the models. The light transmittance was 5 - 10% and it was the highest in model I, where the height of the shading facility was the highest. Rain leakage was 10 - 36%, the most in model Ⅱ. The amount of leaks increased with the amount of precipitation per hour. In all models, ginseng stem and leaf were damaged due to excessive water leakage. On July 10, stem and leaf were grown well only in wide-shading facility where leakage was adequately blocked with double-sided film. The average wind speed was less than 1.6 m/s during the survey period. The maximum wind speed was 8.8 m/s or less, which did not affect the deformation of the wide-shading facility. In all models, there was no difference in growth of aboveground part and root. Conclusion : As a result, in order to grow ginseng continuously and stably using wide-shading facilities, it is considered that the amount of leakage should be minimized by using material that can prevent leakage in cope with rainfall or heavy rain rather than wind-resistance.

Background : Development of new real-time PCR diagnosis method for simultaneous diagnosis of Cylindrocarpon destructans and Fusarium solani, causative fungi of ginseng root rot disease. C. destructans and F. solani are known to be the major pathogens of ginseng root rot disease. Root rot caused by these pathogens is a disease that is difficult to control because the disease progresses slowly and it is difficult to diagnose early and even when symptoms of plant seeding are present, the disease is already spread in the roots. Diagnostic methods to detect the presence or absence of ginseng roots rot fungi in soil before ginseng cultivation are currently being used as a method for controlling. However, commercialized soil extraction kits and PCR diagnostics have cost, diagnostic time, and single diagnostic problems, and need to develop new diagnostic methods. Methods and Results : Primers and probes in the beta-tubulin 2 gene were designed for species-specific detection. In silico analysis, the detection rate of C. destructans was 100% and the detection rate of F. solani was 95%. The multiplex real time PCR optimization conditions including the internal control were established. The analytical sensitivity using positive samples was 10 copies/㎕ for C. destructans and 10 copies/㎕ for F. solani. As a result of performance comparison test with conventional PCR diagnosis methods, it was confirmed that the developed multiplex real time PCR method has the same or better performance in terms of sensitivity. In the developed soil extraction kit, the extraction time was reduced and the extracted DNA quality was improved, compared to the used soil extraction kit. Conclusion : From the above results, we expect that the developed C. destructans / F. solani multiplex real time PCR diagnosis method and soil extraction kit will be useful for real-time monitoring of ginseng root rot pathogenic fungi in the soil of ginseng cultivation area and diagnosis of suitability of ginseng cultivation area.

Background : This study examines the main growth characteristics, physiological stress, or diseases damage for 3 and 4-years-old ginseng in the new lines cultivated in Gyeonggi for development of new lines of Ginseng. Methods and Results : Eumseong 13, 14 and 15, which are two-years-old seedling in the new line ginseng were transplanted in 7 lines × 9 rows (70 plants)/1.62㎡ in 2015 along with Gumpoong and Yeonpoong as the control lines while Eumseong 16 and 17 as well as Chunpoong as the control line were transplanted in the same way in 2016. The ginseng shading was installed with the front post of 170 ㎝ high and the rear post of 100 ㎝ high using the rear line while as the sun blocking material, the aluminum shade plate was used. As for the new growth lines of 3 and 4-years ginseng in the new growth line, the aerial parts of the ginseng and underground part of ginseng were examined. In addition, the physiological stress such as the leaf discoloration, rusty root and root rot as well as the resistance against the disease were examined. It was found out that as for the aerial parts of 3-years-old ginseng in test line, Eumseong 16 and 17 were better in the growth than the control lines were while in 4 years old ginseng, Eumseong 13 and 14 were better in the growth than Gumpoong as the control line was. As for the root weight of the 4-years-old ginseng for the underground part, there was no significant difference compared with control line while in 3-years-old ginseng, Eumseong 16 and 17 were heavier. The leaf discoloration did not happen to both 3 and 4-years-old ginseng. As for the root rot, 3-years-old Eumseong 16 and 17 suffered less root rot in the same way as for the control line. 4-years-old Eumseong 14 and 15 had less root rot in the same way as for the control line. 3-years-old Eumseong 16 and 17 have less rusty root in the same way as the control line while as for 4-years-old ginseng, Eumseong 13, 14 and 15 showed a lot of rusty roots in the same way as the control line. Conclusion : Finally, considering the growth characteristics, physiological stress and root rusty of the aerial parts and underground parts of the ginseng, we selected Eumseong 16 and 17 as the excellent lines for 3-years-old ginseng.

Background : Ginseng is an important agriculture plant in Korea. However, plant yield is reduced by pathogens. Cylindrocarpon destructans and Fusarium solani are responsible for root-rot and replant failure of ginseng in Korea. Because of root rot pathogen, the productivity decreased and repeated cultivation is difficult. Methods and Results : The number of Cylindrocarpon destructans and Fusarium solani in soils can be measured by real-time PCR. This methode makes it possible to select of land for caltivation of ginseng. The specific primers of C. destructans and F. solani were synthesized from β-tubulin region. The equation of the standard curve between the colony forming unit(cfu) and the Ct value in the C. destructans was y (Ct value) = -1.608X (cfu) + 39.325. The equation of the standard curve between the colony forming unit (cfu) and the Ct value in the Fusarium solani was y (Ct value) = -1.608X (cfu) + 39.077. This method makes possible to rapidly exactly measure the number of pathogens in soil. C. destructans, a ginseng root rot fungus, was detected in soil samples of 32 (16%) in soil samples. 35.5% of paddy field, 34.3% of paddy field, 64.1% of field, and 65.6% of paddy field were found in perennial plant. Conclusion : As a result, the major causative agent of ginseng root rot was Cylindrocarpon and the onset density was 102 cfu/g in soil. There was no significant difference in density between fusarium and disease.