A strain of Bacillus thuringiensis, named Bt 1-3, was isolated from Korean soil sample and it showed high insecticidal activity against Plutella xylostella. Bt 1-3 was deterimined to belong to ssp. aizawai (H7) by an H antiserum agglutination test and produced bipyramidal-shaped crystal proteins. PCR analysis with specific cry gene primers showed that Bt 1-3 contained cry1Aa, cry1Ab, cry1C, cry1D and cry2Ab genes. In addition, this isolate showed high uptake rate of foreign plasmid by electroporation. Based on these characteristics of Bt 1-3, we tried to construct a spore-free Bt 1-3 mutant by knock-out sigG gene, which is known as a key transcription factor during sporulation. First, we constructed a basal vector, named pDST, consisting of erythromycin resistant gene (EmR), partial polyhedrin gene and temperature sensitive origin of replication gene (Orits). Subsequently, according to the chromosomal DNA sequence of Bt subsp. konkukian 97-27, we amplifed upstream and downstream regions of Bt 1-3 sigG, and cloned into pDST (pDST-G). So far, several EmR colonies were obtained by electroporating into the wildtype Bt 1-3 and crossover by homologous recombination is going on.

To develop an improved baculovirus insecticide with additional advantages, a novel recombinant baculovirus, AcB5B-AaIT was constructed. B. thuringiensis crystal protein gene (cry1-5) and insect-specific neurotoxin gene (AaIT) were introduced into Autographa californica nucleopolyhedrovirus genome by fusion of polyhedrin-cry1-5-polyhedrin under the control of polyhedrin (polh) gene promoter, and AaIT under the control of early promoter of ORF3004 from Cotesia plutellae bracovirus, respectively. About 150 kDa of Polyhedrin-Cry1-5-Polyhedrin fusion protein expressed by AcB5B-AaIT was occluded into the polyhedra produced by the recombinant virus, and activated as about 65 kDa of crystal protein when treated with gut-juice of Bombyx mori. The AcB5B-AaIT showed about 50% reduced LT50 value compared to that of the recombinant virus, Ap1Ac, expressing Cry1Ac against Plutella xylostella larvae. In addition, Spodoptera exigua larvae fed the recombinant polyhedra of AcB5B-AaIT showed about 4 fold higher refusing diet effect compared S. exigua larvae fed the recombinant polyhedra of the recombinant virus, Ap1C, expressing Cry1C. AcB5B-AaIT could be transferred to wild-type baculovirus along with serial passage by the homologous recombination between two polyhedrin genes contained in polh-cry1-5-polh fusion protein gene. These results suggested that the novel recombinant baculovirus, AcB5B-AaIT, could be applied as advanced viral insecticide.

Carbon/Carbon composite was been manufactured by the technology of warmer-molding process of clutter chopped carbon fiber, using phenolic resin as an adhesive. The degree of graphitization, the microstructure and the friction properties were studied. The results show that the clutter chopped carbon fiber fully scatter in the Carbon/Carbon composite and the degree of graphitization of phenolic resin can reach up to 86.2%, this matrix carbon can form the continuous and stable graphitic thin film on the friction surface during braking process so that the composite has fine friction properties and low wear rate.

In the context of the pan entertainment era, the IP economy has unleashed infinite vitality and brought infinite possibilities to China’s animation market. With the help of the IP economy, China’s anime works will achieve three-dimensional and multi-dimensional development, and also be endowed with emotional needs, establishing an intangible bond with users. This article will explore how Chinese anime works can present a visual feast that combines Chinese characteristics and aesthetics under the IP economy model.

Purpose - Along with Chinese exchange rate's reform advancement, the issue of exchange rate of RMB has increasingly become the heated focus in the world. In July 2005, China carried out the reform of the exchange rate system, and this behavior has aroused the attention of the world. However, the dispute on whether the theory of purchasing power parity holds or not in China still exists. As such, this paper will attempt to explore whether the purchasing power parity is significant in China. Research design, data, and methodology – The monthly data from July 2005 to December 2017 will be employed to analyze the nominal exchange rate of RMB against the US dollar and the nominal exchange rate of RMB against the euro. Based on these datum, an empirical analysis will be conducted under the unit root test and the cointegration test to exploit the significance of purchasing power parity in China. Results - The findings of this paper reveal that an increase in China’s consumer price index will lead to an increase in the RMB exchange rate, which will lead to the depreciation of RMB. Concomitantly, an increase in the consumer price index in the US and Europe will result in a decrease in the RMB exchange rate, which will lead to an appreciation of RMB. In general, in terms of the US, if US consumer price index increases by 1%, China's nominal exchange rate against US dollar will decrease by 0.905%; if China's consumer price index increases by 1%, China's nominal exchange rate against US dollar will increase by 0.648%. In terms of Europe, if Europe consumer price index increases by 1%, China's nominal exchange rate against euro will decrease by 0.277%; If China's consumer price index increases by 1%, China's nominal exchange rate against euro will increase by 0.235%. Conclusions – Generally speaking, the empirical evidences this paper provided show that the purchasing power parity theory has a certain explanatory ability for the decision of RMB exchange rate. As such, the purchasing power parity cannot hold completely, and China's government should continue to deepen the reform of the exchange rate system to improve China's exchange rate market.

At present, global warming has posed a greats challenge to human development and survival. The shortage of traditional fossil energy, such as oil and coal, is becoming ever serious. Against such backdrop, low-carbon economy comes into being. However, "low-carbon" economy first appeared in the official document "2003 White Paper" Our Energy Future: Creating Low-Carbon Economy". In 2006, the Stern Report, led by former World Bank Chief Economist Nicholas Stern, pointed out that the global investment of 1% of GDP per year could avoid the loss of 5% to 20% of GDP in the future, and it called for the global transition to a low-carbon economy. In July 2007, the United States Senate proposed the Low-Carbon Economy Act, indicating that the development path of low-carbon economy is expected to serve as an important strategic choice for the United States in the future. In 2008, the United Nations Environment Programme (UNEP) designated the theme of World Environment Day as "Transforming Traditional Concepts and Promoting a Low-Carbon Economy". Low-carbon economy refers to an economic development pattern that, minimizes the consumption of high-carbon energy such as coal and oil and reduces the emission of greenhouse gases by means of technological innovation, institutional innovation, industrial transformation, new energy development and other means, under the guidance of the concept of sustainable development, under the guidance of the concept of sustainable development, so as to achieve a win-win result between economic and social development and ecological and environmental protection.10) Operation and management is a series of management and operation activities carried out within an enterprise for the purpose of smooth implementation and effective adjustment of production, business, labor, financial and other businesses according to the business objectives. Enterprises should pay more attention to the impact of low carbon economy era on China's enterprise management, because it is a challenge as well as an opportunity, which rapidly actively develops the financial risk in the operation and management links, cost management, performance evaluation sets up the culture of enterprise of low carbon and coordinated development, changes the marketing idea, so as to help the enterprise stands on a low carbon economy era for the long-term development.

Price discount is one of the commonly used promotion strategies to increase sales and revenue. If a discount is perceived before the purchase (i.e., pre-purchase discount), consumers are likely to perceive it as a potential gain. If it is noticed after making a regular-priced purchase (i.e., post-purchase discount), consumers may develop negative emotions and attitudes. Based on the rising transparency and omnipresence of price and discount information through web and mobile platforms, we attempt to tackle an understudied topic on the negative effect of post-purchase price discount. Specifically, post-purchase discount information may increase consumers’ perception of monetary loss, which may affect consumers’ decision to return the product, potentially increasing the operating costs borne by retailers. Based on a close scrutinization of the current market environment and previous academic literature, we suggest a novel conceptual framework to understand consumers’ perception, attitude, and behavior (perception of loss, willingness to return) upon perceiving various formats of discount promotion (absolute value vs. percentage discount) posterior to the purchase of a product. We also look at the effect of price level (low-priced vs. high-priced). For marketing practitioners, we intend to suggest optimal promotion formats that can alleviate consumers’ negative perceptions and prevent additional operation costs.

Purpose - Current study aimed at investigating the symbolic and evaluation relevance to global luxury brands as the causes of inducing social identity verification, and also explored whether the social identity verification will affect the attitude toward the brands. Research design, data, and methodology - 323 questionaries from Chinese consumers were used to test hypotheses by structural equation model of AMOS 22.0. Results - First, social identity verification positively affected on the brand attitude. Second, both the symbolic relevance and the evaluation relevance positively affected on social identity verification. Third, the mediation roles of social identity verification were identified. Social identity verification played a full mediation role in the effect of the symbolic relevance on the brand attitude, and played a partial mediation role in the effect of the evaluation relevance on the brand attitude. Conclusions - This study could contribute to the advancement of theory concerned with the roles of consumers’ social identity verification which induces positive attitude toward the global luxury brands. Global brand managers in China should try to search ways by which consumers can feel both the symbolic relevance and evaluation relevance to their luxury brands, and should make efforts to improve the symbolic relevance and evaluation relevance to their brand.