본 연구는 공원자산을 활용한 공원재정비에 의한 공간구성의 변화가 이용형태 및 이용만족도에 미치는 영향을 파악하여, 향후 한국의 도시공원의 재정비사업의 계획론적인 관점에서의 방향성을 제시하기 위해, 요코하마시 카나자와구의 토미오카 no.5 공원의 재정비사례를 대상으로 2회의 걸친 공원 이용자 설문지 조사와 지역주민, 행정 담당자, 재정비공원 설계자를 대상으로 청취 조사를 실시하였다. 조사를 통해 확인 된 결과를 분석하여 향후 한국의 도시공원 재정비사업의 방향성에 대해 다음과 같이 제시할 수 있다.첫째, 향후 공원재정비 사업의 적극적인 추진이 예측되는 한국에 있어서 공원자산에 관한 이용자의 인식도조사를 통해 파악된 공원자산을, 적극적으로 보존 및 활용한 재정비 수법은 공원의 평가와 만족도향상에 유효하며, 공원 자산을 파악하기 위해 이용자에 대한 인식도 조사가 필요하다고 할 수 있다. 둘째, 재정비를 위한 주민설명회에서 확인 된 공원에 대한 지역주민의 요구와 지자체의 공원재정비의 목적과 과제의 파악을 통해, 이에 맞춰 명확한 설계의도를 설정하는 것은 이용자 만족도 향상에 효과적인 재정비 사업의 프로세스라고 볼 수 있으며, 재정비를 포함한 도시공원정비 사업추진에 시민참여가 활발히 이루어지고 있는 한국에 시사하는 바가 크다고 볼 수 있다.

The canine major histocompatibility complex (MHC) is referred to dog leukocyte antigens (DLA), which is known to be the most polymorphic genetic system in canine species. Many cloned dogs have been produced since Snuppy, first cloned dog, there was no research about genetic identity of MHC among cloned animals. Recently in Lee’s group, two non-transgenic cloned beagles (BG1, 2) were produced by somatic cell nuclear transfer (SCNT) using fetal fibroblast (BF). Also, four transgenic cloned beagles (Ruppy 1-3, 5) were generated using transgenic BF transfected with Red fluorescent protein (RFP) gene. We hypothesize that non-transgenic (BG1, 2) and transgenic (Ruppy 1-3, 5) cloned beagles derived from identical donor cells have the same immunological genetic characteristic except for RFP gene insertion in the genome. Thus, the aim of this study is to confirm the immunological identity of DLA class II in cloned beagles produced using same nuclear donor cell. Genomic DNA was extracted from blood of BG1, BG2, Ruppy 1, 2, 3 and 5. Genomic DNA of normal two control beagle, no correlation with BF was also investigated for rulling out the possibility that beagles were inbred. Forward and reverse primers used for DLA-DQA1 and DQB1 respectively were DQAF: 5’-TAAGGTTCTTTTCTCCCTCT-3’ and DQAR: 5’-GGACAGATTCAGTGAAGAGA-3’ DQBR:5’-CTCACTGGCCCGGCTGTCTC-3’ and DQBR: 5’-CACCTCGC CGCTGCAACGTG-3’. Polymerase Chain Reaction (PCR) products were purified, sequenced directly using the Big Dye Terminator kit. Sequencing analysis was performed on an automated 3730xl DNA analyzer. In experiment 1, sequence of DLA-DQ alpha 1 (DQA1) and DLA-DQ beta 1 (DQB1) exon 2, hypervariabel region, was compared in BG1 and BG2. Experiment 2 also compared the sequence of DQA1 and DQB1 among Ruppy 1, 2, 3 and 5. Experimental 3 compared sequence of DQA1 and DQB1 among all cloned dogs (BG1, BG2 and Ruppy 1-3, 5). As a result, BG1 and BG2 have same allele for DQA1 and DQB1 as we expected. They share DQA1*00101 and DQB1*02901 in experiment 1. In experiment 2, Ruppy 1, 2, 3 and 5 also have identical DQA1*00101 and DQB1*02901 allele. No discrimination between transgenic dogs and cloned dogs was seen in DQA1 and DQB1 Allele in experiment 3. DQA1, DQB1 allele was identified as *00101 and *02901 in all dogs. We provided the allele identity of DQA1and DQB1 in cloned beagles, which can be used as preliminary data for immunological related studies. In conclusion, transgenic cloned dogs despite of red fluorescent protein genes being inserted in their nuclear DNA were immunologically compatible with non-transgenic cloned dogs. We demonstrated that cloned beagles produced using identical nuclear donor were immunologically compatible.

Achyrantes japonica (AJ) has been used to treat edema and arthritis in the traditional Korean medicine. Toelucidate the anti-inflammatory and anti-nociceptive effects of ethanol extract of AJ, the carrageenan-induced pawedema using a plethysmometer and thermal hypersensitivity using the plantar test were measured. Ibuprofen was used asa control drug. Treatment with AJ (200㎎/kg p.o.) significantly reduced paw edema, compared to the carrageenan -treated rats. In the plantar test, the thermal withdrawal latency in AJ - treated group was significantly increased than thecarrageenan - treated group. The results indicate that AJ could have be the anti-inflammatory and anti-nociceptive prop-erties.