Most vacuolar proteins are synthesized on the endoplasmic reticulum (ER) as a proprotein precursor and then transported to vacuoles. In vacuoles, they are converted into the respective mature form. TaVPE1 and TaVPE2 were isolated from the cDNA library that was prepared from the wheat kernels at 16 and 18 days after fertilization (DAF). Additionally, putative TaVPEs (TaVPE3, TaVPE4) were isolated by inverse PCR (IPCR) using GrainGenes database. Each open reading frame (ORF) encodes 495, 497, 495, 456 amino acids, respectively. TaVPEs were closely related in respect to peptide comparisons and their sequence homologies were ranged from 84% to 93%. The TaVPE genes showed various expression patterns in response to exogenous treatment of phytohormones. The transcript of TaVPE1 was detected slightly and steadily after exposure to all phytohormones and the accumulation of TaVPE2 transcript was increased from 24 hours in NaCl treatment. The transcript of TaVPE3 was increased from 48 hours in response to H2O2 and decreased after exogenous application of ABA and salicylic acid. In case of TaVPE4, the transcript of TaVPE4 were weakly detected all time points of each phytohormone treatment.
A new sprout-soybean cultivar, "Pungwon" was developed at the Honam Agricultural Research Institute (HARI), and National Institute of Crop Science (NICS) in 2006. "Pungwon" was selected from a cross between S193001 and Suwon 164. The preliminary, advanced, and regional yield trials for evaluation and selection of this line (Iksan 52) were carried out from 2002 to 2006. This cultivar has a determinate growth habit, white flower, grayish brown pubescence, yellow seed coat, yellow hilum, lanceolate leaflet shape and small seed size (10.9 grams per 100 seeds). The maturity date of "Pungwon" is seven days earlier than that of the check variety, "Pungsan". It has a good seed quality for soybean-sprout. The soybean-sprouts grown from "Pungwon" have high isoflavone (5,935 μg/g) and amino acid (160 mg/g) contents. This cultivar has resistance to soybean mosaic virus (SMV), necrotic symptom (SMV-N), and other most troublesome soybean diseases which are bacterial pustule and black root rot. Especially, "Pungwon" has useful characteristics, such as lodging and shattering resistance. The average yield of "Pungwon" was 3.04 ton per hectare, about 7 % higher than the check variety, "Pungsan" at the regional yield trials (RYT) for double cropping.
A new sprout-soybean cultivar, "Jonam" was developed at the Honam Agricultural Research Institute (HARI) in 2006. Jonam was selected from a cross between Eunhakong and Jeonju-I1. The preliminary, advanced, and regional yield trials for evaluation and selection of this line (Iksan 48) were carried out from 2002 to 2006. This cultivar has a determinate growth habit with purple flower, grayish brown pubescence, grayish brown hilum, lanceolate leaflet shape and small seed size (9.6 grams per 100 seeds). The maturity date of "Jonam" is 18 days earlier than that of the check variety, "Pungsan". It has good seed quality for soybean-sprout and resistance to lodging. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom(SMV-N). The average yield of "Jonam" was 2.57 ton per hectare in the regional yield trials for double cropping carried out for three years from 2004 to 2006.
A new soybean cultivar for soy-paste, “Mansu”, was developed from the cross between ‘Suwon 192’, which was tolerant to disease, late maturing and high yielding, and ‘Suwon 196’ which was early maturing and large seed by soybean breeding team in the Yeongnam Agricultural Research Institute (YARI) to make new variety having large seed and high yielding. A promising line, SS97213-2B-3SSD-39-1-1, was selected and designated as the name of ‘Suwon 236’. It was characterized by regional yield trials (RYT) for three years from 2004 to 2006 and released as the name of “Mansu”. It has a determinate growth habit, purple flower, grey pubescence, pale green seed coat, yellow cotyledon, large spherical seed (26.5 grams per 100 seeds). Maturity date is 4 days later than the check cultivar, Taekwang. The average yield of Mansu was 2.93 ton per hectare in the regional yield trials (RYT) carried out for three years from 2004 to 2006 which was 7 percent higher than that of the check cultivar, Taekwang.
‘Seohong’ was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made in 1997 between ‘Jashim’ as a female and 93K65-1 as a male parent. Seohong was evaluated as a seedling number 97S05-1 for tuber characteristics, growth and yielding ability from 2001 to 2002, and than this clone was renamed as Daegwan 1-82. This clone was evaluated in the regional yield trial and the disease resistance in the five locations for three years. Finally, this clone was named as ‘Seohong’ and registered a new recommended potato cultivar in 2006. This cultivar has a round, shallow eye depth, light red-skinned tuber characteristics and low to medium dry matter content. It showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to potato virus Y (PVY) and late blight (Phytophthora infestans). Its mean tuber yields from the regional yield trial and winter cropping trial were 12 and 49 percent higher than that of ‘Superior’, respectively.