본 연구는 오존화 올리브오일이 독성이 적으면서 다양한 종류의 미생물을 치사시킬 수 있으나 경우투여나 복강투여에도 인체에 해가 없는 것으로 알려져 있다. 그리고 국내에서는 아직 동물에 대한 안정성 실험이 없었다. 그래서 본 연구에서는 오존화 올리브오일을 사용하여 동물의 안구를 통한 안정성 실험을 하였다. 구체적인 연구에서는 rabbit에서 오존화오일(고농도)의 동물 눈 안점막 자극을 평가하기 위해 실시하였고, 시험물질 처치부위를 관찰한 결과, 비세척군 및 세척군 모두에서 안점막 자극이 관찰되지 않았다.

본 연구는 오존수를 이용한 인체공학적 샴푸대가 분리 가능하고 미세분사 형식의 샤워헤드 와 높낮이 조절이 가능한 샴푸대를 개발할 수 있는 장치기술 및 그 방법을 제시하고자 한다. 개발결과 기존의 장치보다 피시술자 머리가 세발대 안으로 들어오는 인체공학적 디자인과 목받이 부분을 높게 제작하여 물튀김 방지를 완성하였다. 실험결과 물을 공급하는 통에 부착되어 있는 온수 유지 히터를 통 해 피시술자의 기호에 맞는 온도를 설정하여 시술 시 지연 시간 없이 온수 세발 가능하도록 온도센서를 통하여 확인한 결과 수온유지(38℃)가 일정하게 유지되었다. 그리고 오존수 변환장치 설치로 자체 살균 (1PPM) 및 정화 기능까지 가능한 장치의 효용성을 알 수 있었다. 오존수를 20분 정도 측정한 결과 오 존 농도가 1PPM 이하로 측정되어 안정성을 확보하였다. 최종적으로 오존수를 20분 정도 측정한 결과 오존 농도가 1PPM 이하로 측정되어 안정성을 확보하였고, 온수장치와 오존수 변환장치와 함께 이동 샴 푸대의 모든 부분이 사용자가 사용하기에 불편함이 없도록 설계 하였다.

본 연구는 생활주변에서 오존수를 쉽게 활용할 수 있도록 오존수 발생장치의 활용성을 증가시키고 한 동작으로 작동하는 시스템을 개발하고자 한다. 더 나아가 올리브 오일과 오존과 반응시켜 제조하는 기술에 적용하고자 한다. 기존의 경우 대부분이 오존수 시스템이 아닌 오존발생기 위주의 제품이 많다. 오존수기의 경우 펌프로 강제 흡입시키는 일반압력 방식으로 오존가스 누출 위험요소가 내포 되어 있으나, 본 과제의 경우 인젝터를 이용하여 물의 유입여부에 따른 오존의 발생과 흡입을 제한하고 있어 기존 제품의 오존 누출에 의한 위험을 최소화하였다. 인젝터 방식의 단점에도 불구하고 미생물 살균능을 유지하는 것으로 본 연구에서 사용된 인젝터 방식 오존수 제조 장치의 효용성을 알 수 있었다.

탱자에는 여러 종류의 monoterpenes, limonoids, flavonoids과 coumarins 등의 구성성분으로 이루어져 있다. 탱자의 구성 성분중에 7-제라닐옥시쿠마린는 7개의 탄소로 구성된 제라닐옥시기의 곁사슬을 가진 물질이다. 그리고 7-제라닐옥시쿠마린은 여러 약리적인 효과들을 나타낸다고 알려져 있다. 본 연구에서는 탱자의 구성성분인 7-제라닐옥시쿠마린과 이들의 다양한 유도체를 합성하였다. 그리고 항염증에 대한 항염증 효능을 알아보기 위하여 염증을 유발하는 일산화질소 억제 cytokine을 측정한 결과는 6-제라닐옥시쿠마린의 성분이 사이토카인인 인터루킨-6가 1μm농도에서 68.9% 를, 10μM에서 72.6% 의 저해효과를 나타냈다.

본 연구에서는 TiO2 입자를 얻을 수 있는 침전법을 이용하여 TiO2 입자를 제조하였다. TiO2 입자 제조시 사용되는 알콜 용매의 종류와 온도 변화 등의 매개변수가 TiO2 입자의 결정 구조, 입자의 크기 및 형태에 미치는 영향을 조사하였다. TiO2 입자제조시 용매로 사용한 알콜 종류인 methyl alcohol, iso-propylalcohol, 그리고 tert-butylalcohol를 scanning electron microscope(SEM) 분석한 결과 iso-propylalcohol이 가장 좋은 결과를 가져왔다. 그리고 온도 변화를 열분석법을 사용한 결과 200℃에서 500℃까지는 아나타제 구조를 유지하였으나, 800℃에서는 루틸 구조로 전환되었다.

In this study, we evaluated the anti-oxidative, whitening and anti-wrinkle effect of Tetrapanax papyriferum extract. Tetrapanax papyriferum was extracted by two different solvents which were n-Hexane, ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase, elastase for anti-wrinkle were investigated. For anti-oxidant activity, ethyl acetate fraction of Tetrapanax papyriferum extract showed more significant activity than n-Hexane fraction of Tetrapanax papyriferum extract. For whitening activity, n-Hexane fraction of Tetrapanax papyriferum extract exhibited strong inhibition effects compared with reference. Therefore, Tetrapanax papyriferum extract may be useful as a new antioxidant and whitening agent.

Coumarin derivatives were shown to possess valuable pharmacological properties such as anticancer/anti carcinogenic, anti-inflammatory, anti helicobacter, anti genotoxic, neuroprotective and dietary effect. In this study, novel coumarin derivatives structurally related to 7-geranyloxycoumarin were effectively synthesised in good yields by Cs2CO3/acetonitrile in mild condition. The synthesis of geranyloxycoumarin derivatives in weak base(Na2CO3, K2CO3, Cs2CO3 etc)/CH3CN at room temperature obtained in good yield. On the other hand, the reaction of geranyloxycoumarin formation in strong base(NaOH, KOH, CsOH etc)/CH3CN at reflux condition obtained in low yield.

We investigated the electrochemical properties for Langmuir-Blodgett(LB) films mixed with l-bromotetradecane(Cl4), l-bromohexadecane(Cl6), and l-bromooctadecane(Cl8). The alkyl bromides mixture was deposited by using the Langmuir-Blodgett method on the ITO glass. The electrochemical properties measured by using cyclic voltammetry with a three-electrode system(an Ag/AgCl reference electrode, a platinum wire counter electrode and LB film-coated ITO working electrode) at various concentrations(0.5, 1.0, 1.5 and 2.0 N) of NaClO4 solution. A measuring range was reduced from initial potential to -1350 m V, continuously oxidized to 1650 mV. The scan rate was 100 mV/s. As a result, LB films of Cl4, Cl6, and Cl8 mixture monolayers appeared irreversible process caused by only the oxidation current from the cyclic voltammogram. The diffusivity(D) effect of LB films decreased with increasing of alkyl bromides amount.

Core-Shell polymers of silicone dioxide-styrene system were prepared by sequential emulsion polymerization. In inorganic/organic Core-Shell composite particle polymerization, silicone dioxide adsorbed by surfactant sodium dioctyl sulfosuccinate (EU-DO133L) was prepared initially and then core silicone dioxide was encapsulated emulsion by sequential emulsion polymerization using styrene at the addition of potassium persulfate (KPS) as an initiator. We found that SiO2 core shell of SiO2/styrene structure was formed when polymerization of styrene was conducted on the surface of SiO2 particles, and the concentration sodium dioctyl sulfosuccinate (EU-DO133L) was 0.5~2.0g. The structure of core-shell polymer were investigated by measuring to the thermal decomposition of polymer composite using thermogravimetric analyzer and morphology of latex by scanning electron microscope(SEM).

Silicone dioxide absorbed polyoxyethylene alkylether sulfate (EU-S75D) surfactant was prepared. The core-shell composite of inorganic/organic were polymerized by using styrene(St) as a shell monomer and potassium persulfate(KPS) as an initiator. We studied the effect of surfactants on the core-shell structure of silicone dioxide/styrene in the presence of an anionic surfactant lauryl sulfate(SLS). The structure of core-shell polymer were investigated by measuring to the thermal decomposition of polymer composite using thermogravimetric analyzer(TGA) and morphology of latex by scanning electron microscope(SEM).

Titanium dioxide particles are used as photocatalysts, sensors, adsorbents and catalyst. Core-shell polymers of inorganic/organic pair, which have both core and shell component, were synthesized by sequential emulsion polymerization using Acrylate as a shell monomer and potassium persulfate (KPS) as an initiator. We found that when Acrylate core prepared by adding 0.5~2.0 wt% EU-S133D, Titanium dioxide / Acrylate core-shell polymerization was carried out on the surface of Titanium dioxide particle without forming the new Titanium dioxide particle during acrylate shell polymerized in the inorganic/organic core-shell polymer preparation. The structure of core-shell polymer were investigated by measuring to the thermal decomposition of polymer composite using thermogravimetric analyzer(TGA) and morphology of latex by scanning electron microscope(SEM).

Esterification reaction between succinic acid and 1,4-butanediol was kinetically investigated in the presence of monobutyl tinoxide catalysts at 150~190℃. The reaction rates measured by the amount of distilled water from the reaction vessel. The esterification reaction was carried out under the first order conditions with respect to the concentration of reactants, respectively. The overall reaction order was 2nd. The linear relationship was shown between apparent reaction rate constant and reciprocal absolute temperature. By the Arrhenius plot the activation energy have been calculated as 87.567 kJ/mol under monobutyl tinoxide catalyst and also apparent reaction rate constant, k' was found to obey first kinetics with respect to the concentration of catalyst.

Silicone dioxide absorbed polyoxyethylene alkylether sulfate (EU-S133D) surfactant was prepared. Core-shell polymers of inorganic/organic pair, which have both core and shell component, were synthesized by sequential emulsion polymerization using Acrylate as a shell monomer and potassium persulfate (KPS) as an initiator. We found that when Acrylate core prepared by adding 2.0 wt% EU-S133D, silicone dioxide/Acrylate core-shell polymerization was carried out on the surface of silicone dioxide particle without forming the new silicone dioxide particle during acrylate shell polymerization in the inorganic/organic core-shell polymer preparation. The structure of core-shell polymer were investigated by measuring to the thermal decomposition of polymer composite using thermogravimetric analyzer and morphology of latex by scanning electron microscope(SEM).

Behaviors of saturated fatty acid halides (CI4, C16, C18) were measured by LB method when the molecules were stimulated by pressure. The saturated fatty acid halides were deposited on the indium tin oxide(lTO) glass by the LB method. The average organic ultra thin film size and the surface roughness of the fatty acid halides thin films were investigated using AFM. It was found that AFM images show small surface roughness (2.5~5.0 nm) and the organic ultra thin film size of 2.5~12 nm. Both aggregations and pin-holes were also seen on the AFM images. However we found that the surface roughness. These effects seem to be reasonable to be related to the increase of the organic ultra thin film size of fatty acid halides.

The effects of the applied stretch and MgADP binding on the structure of the actin and myosin cross-bridges in rabbit fibers in the rigor state have been investigatedwith improved resolution by x-ray diffraction using synchrotron radiation. To clarify the structure of the ATP hydrolysis intermediates formed by actin and myosin cross-bridges,the effects of various phosphate analogs in the of MgADP on the structure of the thin and thick filaments in glycerinated rabbit muscle fibers in the rigor state investigated by x-ray diffraction with a short exposure time using synchrotron radiation. These results strongly suggest that when MgADP and phosphate analogs such as metallofluorides(BeF3 and AlF4)and vanadate(VO4(Vi)) were added the rigor fibers in the presence of the ATP-depletion backup system, the intensities of the actin-based layer lines were markedly weakened. We found that the intensity of the 14.5 nm-based meridional reflections increase by 20-50% when phosphate analogs such as metallofluorides(BeF3 and AlF4) and vanadate(VO4(Vi)) was added to the rigor muscle.

To study the relationship between elementary biochemical states and structural states of the actomyosin crossbridges in muscle, the effects of binding of MgADP to myosin heads in the rigor muscle were examined by X-ray diffraction using synchrotron radiation. X-ray diffraction studies have been made to investigate the effects of binding of ADP on the structure of glycerinated rabbit skeletal muscle in the rigor state. The intensity increase was accompanied by a slight but distinct decrease in the 5.9 am layer-line intensity close to the meridian. These results strongly suggest that myosin heads altered their attached conformation in the proximal end toward the plane perpendicular to the fiber axis when MgADP was bound to them. We found that the intensity of the 14.5 nm-based meridional reflections increase by 20-50% when MgADP was added to the rigor muscle in the presence of hexokinase and myokinase inhibitor.

X-ray diffraction studies have been made to investigate the effects of binding of ADP, ADP+Vi, ADP+AIF4, ADP+BeF3 on the structure of glycerinated rabbit skeletal muscle in the rigor state. Although these phosphate analogs are known to bind actively cycling myosin heads, it is not clear whether they can bind to the attached heads in the rigor muscle. We have found that these analogs can bind to the myosin heads attached to actin filaments in the rigor state. The present results indicate that (1) bound myosin heads altered their conformation in the proximal end toward the plane perpendicular to the fiber axis when MgADP bound to them, and (2) myosin heads were dissociated substantially (up to 50%) from actin filaments but still remained in the vicinity of actin filaments when MgADP and metallofluorides (AIF4 and BeF3) or vanadate bound to them. We detected new conformations of myosin heads attached to actin filaments when they had MgADP or ADP.Pi analogs. We report here these findings on the effects of MgADP and MgADP+phosphate analogs to the rigor crossbridges.

Core-shell polymers of methyl methacrylate-styrene system were prepared by sequential emulsion polymerization in the presence of sodium dodecyl benzene sulfonate(SDBS) as an emulsifier using ammonium persulfate(APS) in an initiator and the characteristics of these core-shell polymers were evaluated. Core-shell composite latex has the both properties of core and shell components in a particle, whereas polymer blends or copolymers show a combined physical properties of two homopolymers. This unique behavior of core-shell composite latex can be used in various industrial fields. However, in preparation of core-shell composite latex, several unexpected matters are observed, for examples, particle coagulation, low degree of polymerization, and formation of new particles during shell polymerization. To solve this matters, we study the effects of surfactant concentrations, initiator concentrations, and reaction temperature on the core-shell structure of PMMA-PSt and PSt-PMMA. Particle size and particles distribution were measured by using particle size analyzer, and the morphology of the core-shell composite latex was observed by using transmission electron microscope. Glass temperature was also measured by using differential scanning calorimeter. To identify the core-shell structure, pH of the composite latex solutions was measured.

IASL(iodo acetamide) and MSL(maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. Equatorial refiection(10,11) change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of 143a and 72a could offer information of the mass projection of population of myosin heads along the :filament axis. The slope of intensity profile of the mass projection of 143a and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of 215a reflection intensity is appeared the periodical characteristic of 143a reflection by spin label. The raise of MSL actin reflection at 51a and 59a in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we could conclude that LASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.

On contraction of the muscles, marked changes in X-ray reflections are observed, suggesting that conformational changes of contractile molecules and the movement of myosin heads during muscle contraction. Time slice requires tension peak after the onset of stimulation and the height of tension peak depends on the number of twitch cycle. The muscles were stimulated by five successive stimuli at an interval of 80 ms started while the tension was still being exerted by the muscles. The intensity of I11, I10, 143a and 215a reflection measured with 5ms time resolution and is recorded in isometric tension. The peak height of I11 and 143a intensity is changed after the onset of a stimulation Ii, and the length of twitch is shortened by successive twitches in the case of stimulation Ti. On the other hand, the peak height of In and 215a intensity starts to decrease at the 1st twitch and remains constant at low peak height without appreciable recovery during the contraction term. In the case of successive twitch stimulation, the myosin heads of muscle are once moved from their resting position and never returned to their initial position.