The purpose of this study is to monitor the pesticide residues in frozen fruits and vegetables distributed and sold in online and offline markets in Korea. For the study, 107 samples of 34 types of frozen fruits and vegetables were examined, and a total of 341 pesticide residues were analyzed by using multiclass pesticide multiresidue methods of the Korean Food Code. As a result, pesticide residues were detected from 16 of 64 frozen fruits samples and 15 of 43 frozen vegetables samples. Conclusively, residues were detected from 31 samples in total, showing a detection rate of 29.0%. Specifically, pyridaben exceeded the Maximum Residue Limits (MRLs) based on the Positive list system (PLS) in one of the frozen radish leaves, and the violation rate was 0.9%. Detection on frozen fruits and vegetables was made 23 times for 11 types and 36 times for 21 types. In total, 28 types of pesticide residues were detected 59 times. Fungicides were detected the most in frozen fruits, while insecticides were detected the most in frozen vegetables. The most detected pesticides were the insecticide, acaricide chlorfenapyr (5) and the fungicide boscalid (5). Chlorfenapyr was detected only in frozen vegetables, and boscalid was detected in frozen fruits except one.

본 연구에서는 chlorogenic acid 이성질체 9종과 arbutin을 분석하기 위하여, 전처리 과정과 UHPLC-MS/MS 동시 분석 조건을 확립하였다. UHPLC-MS/MS에서 C18 column을 사용하여 15분 동안 분석할 수 있는 용매 조성을 만들고, 10가지 물질에 대한 정량, 정성 이온을 선택하여 negative 모드에서 분석하는 동시분석법을 확립하였고 과일류를 대상으로 추출, 진탕 추출, 초음파 추출, 원심분리, 농축 등의 과정을 거치는 전처리법을 개발하였다. 기기분석과 전처리법에 대한 유효성은 특이성, 직선성, 정확성, 정밀성 그리고 검출한계 및 정량한계 등을 통하여 확인하였다. 회수율은 48.1-120.3%, intraday precision (RSD)는 0.4-7.9%, interday precision (RSD)는 0.0-7.2%로 확인되었다. 따라서, 본 연구에서 확립한 동시분석방법은 과일류 중 chlorogenic acid와 arbutin을 효과적으로 분석하는데 유용할 것으로 사료된다.

PURPOSES: The purpose of this study is to examine public transport service indexes to deal with traffic congestion problems that occur in old industrial complexes during peak hours in an attempt to shift personal car-oriented transportation to public transit-oriented transportation. METHODS : We developed several models to evaluate the effect of public transport service indexes on worke’s mode choice using two primary parameters: overall bus service frequency in old industrial complexes and accessibility to bus stops. Ten old industrial complexes were selected to conduct the survey of workers’commuting patterns. From the survey, personal and travel time attributes were collected and used to estimate a binary logistic model with public transport service parameters. RESULTS : As a result, the mode choice model including the overall bus service frequency variable, better explains than other models indicating providing more frequent bus services to workers in the old industrial complexes can attract them to shift their travel mode to bus transit from personal car. Results can be applied to corresponding city’s bus transit service policy decisions. CONCLUSIONS: In this study, it was possible to estimate the variation of mode choice ratio according to the change of the public transport index, and the mode choice variation by each individual industrial complex can be expected to examine in the further study.

The purpose of this study is to examined the electrofusion and activation conditions for the production of porcine somatic cell nuclear transfer (SCNT) embryos. In this study, immature oocytes were cultured in TCM-199 with and without hormones for 22 hours. Skin fibroblasts cells of porcine were transferred into the perivitelline space of enucleated in vitro matured oocytes. Cell fusion was performed with two different pulses that each one pulse (DC) of 1.1 kV/cm or 1.5 kV/cm for 30 μsec. After fusion subsequent activation were divided into three groups; non-treatment (control) and treatment with 2 mM 6-DMAP or 7.5 μg/ml cytochalasin B for 4 hours. Transferred embryos were cultured in PZM-3 (Porcine Zygote Medium-3) in 5% CO₂ and 95% air at 39℃ for 7 day. Apoptosis-related genes (Caspase-3, BCL-2, mTOR, and MMP-2) were analyzed by immunofluorescence staining. There was no significant difference between two different electrofusion stimuli in the cleavage rate; 64.9±4.8% in 1.1 kV/cm and 62.7±4.0% in 1.5 kV/cm. However, blastocyst formation rate (%) was significantly different among three different activation groups (no treatment, 2 mM 6-DMAP or 7.5 μg/ml cytochalasin B) combined with electrofusion of 1.1 kV/cm. The blastocyst formation rate was 12.6±2.5, 20.0±5.0, and 34.9±4.3% in control, 2 mM 6-DMAP, and 7.5 μg/ml cytochalasin B, respectively. Immunofluorescence data showed that expression levels of caspase-3 in SCNT embryos undeveloped to blastocyst stage were higher than those in the blastocyst stage embryos. Expression levels of Bcl-2 in blastocyst stage embryos were higher than those in the arrested SCNT embryos. These results showed that the combination of an electric pulse (1.1 kV/cm for 30 μsec) and 7.5 μg/ml cytochalasin B treatment was effective for production of the porcine SCNT embryos.

To aid in the development of a food nutrient database that provides the dietary fiber composition of eat-out Korean foods, we determined the total dietary fiber (TDF) content in the eat-out Korean foods Muchim, Bokkeum, and Guk (Tang) using a dietary fiber autoanalysis instrument. A total of 59 samples were collected from Gangwon-do, Gyeonggi-do, Gyeongsangdo, Seoul, Jeonla-do, and Chungcheong-do. First, among 14 samples of Muchim, the TDF content of Kkaennip namul, Goguma-julgi-namul, and Dallae-namul (4.33~6.24, 3.16~5.07 and 3.70~4.99 g/100 g, respectively) was higher than the other types of Muchim. There was no significant difference in TDF content of Muchim (p>0.05) among locations. Among 13 samples of Bokkeum, the TDF content of Pyogo-beoseot-Bokkeum (4.77~6.66 g/100 g) and Miyeok-julgi-Bokkeum (4.16~7.47 g/100 g) was higher than the other types of Bokkeum. The TDF content of Pyogo-beoseot-Bokkeum in Gyeongsang-do was the lowest and the TDF content of Miyeok-julgi-Bokkeum in Gangwon-do was the highest (p<0.05). The TDF content of spinach soybean paste soup (1.34~2.21 g/100 g), Dakgogi-yukgaejang (1.61~2.45 g/100 g), duck stew (1.25~2.80 g/100 g) and spicy yellow croacker stew (1.70~2.27 g/100 g) were higher than the other types of Guk (Tang). There was no significant difference in TDF content of Guk (Tang) among locations (p>0.05).

본 연구는 히어리 개체군의 분포가 북사면에서 제한적으로 분포하는 요인을 구명하기 위하여 실내 실험 및 전라남도 순천지역의 히어리 자생지에서 생육지(남사면과 북사면) 실험를 대상으로 종자 발아실험과 미기상 환경을 조사하였다. 종자 발아실험은 실내 및 생육 현지에서 실시하였고, 미기상 요인 측정은 히어리가 분포하는 지역과 분포하지 않는 두 지점에 대하여 기온, 지온, 습도, 토양수분 및 광량자를 각각 측정하였다. 히어리의 종자는 사면에 따른 환경에는 영향을 받지 않고 발아되어 종자의 산포 및 발아단계에서 사면의 환경 차이가 제한 요소로 작용하지 않는 것으로 판단되었다. 하지만 발아한 유묘가 정착하는 단계에서 북사면이 남사면에 비해 낮은 온도와 높은 습도를 보여, 북사면이 상대적으로 건조에 취약한 유묘의 정착에 용이한 환경을 제공하는 것으로 조사되었다. 정착단계 이후 개체군의 성립 단계에서는 북사면이 남사면에 비하여 낮은 온도와 높은 습도가 유지되었지만 그 차이는 미미하게 나타나 히어리 개체군의 성립에 있어 발아단계에서 유묘로 정착하는 초기단계에서의 히어리 개체군의 사면적 분포특성에 영향을 미치는 중요한 요인으로 작용함을 파악할 수 있었다.